Ian Willis

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. ncbi Genetic interactions of MAF1 identify a role for Med20 in transcriptional repression of ribosomal protein genes
    Ian M Willis
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, United States of America
    PLoS Genet 4:e1000112. 2008
  2. ncbi Integration of nutritional and stress signaling pathways by Maf1
    Ian M Willis
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Trends Biochem Sci 32:51-3. 2007
  3. ncbi Regulation of RNA polymerase III transcription involves SCH9-dependent and SCH9-independent branches of the target of rapamycin (TOR) pathway
    Jaehoon Lee
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Biol Chem 284:12604-8. 2009
  4. ncbi Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription
    Rajendra Upadhya
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, NY, USA
    Mol Cell 10:1489-94. 2002
  5. ncbi Protein kinase A regulates RNA polymerase III transcription through the nuclear localization of Maf1
    Robyn D Moir
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 103:15044-9. 2006
  6. ncbi Sub1 functions in osmoregulation and in transcription by both RNA polymerases II and III
    Emanuel Rosonina
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Mol Cell Biol 29:2308-21. 2009
  7. ncbi Two steps in Maf1-dependent repression of transcription by RNA polymerase III
    Neelam Desai
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:6455-62. 2005
  8. ncbi Signaling repression of transcription by RNA polymerase III in yeast
    Ian M Willis
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 USA
    Prog Nucleic Acid Res Mol Biol 77:323-53. 2004
  9. ncbi The Brf1 and Bdp1 subunits of transcription factor TFIIIB bind to overlapping sites in the tetratricopeptide repeats of Tfc4
    Yanling Liao
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:44467-74. 2003
  10. ncbi A gain-of-function mutation in the second tetratricopeptide repeat of TFIIIC131 relieves autoinhibition of Brf1 binding
    Robyn D Moir
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Biol 22:6131-41. 2002

Research Grants

  1. STRUCTURE/FUNCTION OF POL III TRANSCRIPTION FACTORS
    Ian Willis; Fiscal Year: 2002
  2. SIGNALING PATHWAYS & TRANSCRIPTIONAL REGULATION
    Ian Willis; Fiscal Year: 2003
  3. STRUCTURAL & FUNCTION OF POL III TRANSCRIPTION FACTORS
    Ian Willis; Fiscal Year: 2007
  4. Transcriptional Repression by Maf1 in Yeast
    Ian Willis; Fiscal Year: 2009
  5. STRUCTURE & FUNCTION OF POL III TRANSCRIPTION FACTORS
    Ian Willis; Fiscal Year: 1993
  6. Transcriptional Repression by Maf1 in Yeast
    Ian Willis; Fiscal Year: 2009

Collaborators

  • Charles Boone
  • Gordon Chua
  • T R Hughes
  • Jonathan Warner
  • Pavel Cabart
  • Daniel Kornitzer
  • Deborah L Johnson
  • Robyn D Moir
  • Jaehoon Lee
  • Yanling Liao
  • Neelam Desai
  • Arpita Bhattacharya
  • Rajendra Upadhya
  • Emanuel Rosonina
  • Avrom J Caplan
  • Sandra S Johnson
  • Anthony A Sauve
  • Liping Wu
  • Karen V Puglia
  • Kerri B McIntosh
  • James L Manley
  • Jody Fromm
  • Cheng Zhang
  • Rebecca A Haeusler
  • David R Engelke
  • Vern L Schramm
  • Yaya Chu
  • Vala Thoroddsen
  • Patrick Errada
  • Christine E Bulawa
  • Tony Wood
  • Alexandra E Gould
  • Deborah R Wysong
  • Patrick K Dorr
  • Lawrence R Dick
  • Jing Pan
  • Timothy D Ocain
  • Karen McGovern
  • Tanya Parkinson
  • Ziva Weissman
  • Ronald K Blackman

Detail Information

Publications20

  1. ncbi Genetic interactions of MAF1 identify a role for Med20 in transcriptional repression of ribosomal protein genes
    Ian M Willis
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, United States of America
    PLoS Genet 4:e1000112. 2008
    ..The data suggest that Mediator and Maf1 function in parallel pathways to negatively regulate RP mRNA and tRNA synthesis...
  2. ncbi Integration of nutritional and stress signaling pathways by Maf1
    Ian M Willis
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Trends Biochem Sci 32:51-3. 2007
    ..These new findings indicate that the opposing actions of protein kinase A and protein phosphatase 2A alter the phosphorylation state of Maf1 and thereby regulate its localization and repressing activity...
  3. ncbi Regulation of RNA polymerase III transcription involves SCH9-dependent and SCH9-independent branches of the target of rapamycin (TOR) pathway
    Jaehoon Lee
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Biol Chem 284:12604-8. 2009
    ....
  4. ncbi Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription
    Rajendra Upadhya
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, NY, USA
    Mol Cell 10:1489-94. 2002
    ..Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions...
  5. ncbi Protein kinase A regulates RNA polymerase III transcription through the nuclear localization of Maf1
    Robyn D Moir
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 103:15044-9. 2006
    ..Finally, we report a previously undescribed phenotype for Maf1 in tRNA gene-mediated silencing of nearby RNA pol II transcription...
  6. ncbi Sub1 functions in osmoregulation and in transcription by both RNA polymerases II and III
    Emanuel Rosonina
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Mol Cell Biol 29:2308-21. 2009
    ....
  7. ncbi Two steps in Maf1-dependent repression of transcription by RNA polymerase III
    Neelam Desai
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:6455-62. 2005
    ..The results suggest that Maf1 functions by a non-stoichiometric mechanism to repress pol III transcription...
  8. ncbi Signaling repression of transcription by RNA polymerase III in yeast
    Ian M Willis
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 USA
    Prog Nucleic Acid Res Mol Biol 77:323-53. 2004
  9. ncbi The Brf1 and Bdp1 subunits of transcription factor TFIIIB bind to overlapping sites in the tetratricopeptide repeats of Tfc4
    Yanling Liao
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:44467-74. 2003
    ..The properties of the L469K mutation identify both Brf1 and Bdp1 as ligands for the second TPR array...
  10. ncbi A gain-of-function mutation in the second tetratricopeptide repeat of TFIIIC131 relieves autoinhibition of Brf1 binding
    Robyn D Moir
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Biol 22:6131-41. 2002
    ....
  11. ncbi Tetratricopeptide repeats of Tfc4 and a limiting step in the assembly of the initiation factor TFIIIB
    Robyn D Moir
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Adv Protein Chem 67:93-121. 2004
  12. ncbi Chemical activation of Sir2-dependent silencing by relief of nicotinamide inhibition
    Anthony A Sauve
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cell 17:595-601. 2005
    ..Thus, a nicotinamide antagonist is a Sir2 agonist in vitro and in vivo...
  13. ncbi Facilitated recycling protects human RNA polymerase III from repression by Maf1 in vitro
    Pavel Cabart
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 283:36108-17. 2008
    ..This indicates that recombinant Maf1 is unable to inhibit facilitated recycling. The data suggest that additional biochemical steps may be necessary for rapid Maf1-dependent repression of RNA pol III transcription...
  14. ncbi Interactions of Brf1 peptides with the tetratricopeptide repeat-containing subunit of TFIIIC inhibit and promote preinitiation complex assembly
    Yanling Liao
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Mol Cell Biol 26:5946-56. 2006
    ..The data are consistent with this peptide causing a conformational change in TFIIIC that overcomes Tfc4 autoinhibition of Brf1 binding and suggest a structural model for the Brf1-Tfc4 interaction...
  15. ncbi Autoinhibition of TFIIIB70 binding by the tetratricopeptide repeat-containing subunit of TFIIIC
    Robyn D Moir
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:694-701. 2002
    ..The results demonstrate that the TFIIIB70 binding sites in TFIIIC131 are subject to autoinhibition. We propose that the binding of TFIIIB70 to these sites within the TFIIIC complex may proceed in an ordered fashion...
  16. ncbi Why Dom34 stimulates growth of cells with defects of 40S ribosomal subunit biosynthesis
    Arpita Bhattacharya
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Avenue, Bronx, NY 10461, USA
    Mol Cell Biol 30:5562-71. 2010
    ....
  17. ncbi Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases
    Sandra S Johnson
    Department of Biochemistry and Molecular Biology, Keck School of Medicine and the Norris Comprehensive Cancer Center, University of Southern California, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    Mol Cell 26:367-79. 2007
    ..Together with the ability of Maf1 to reduce biosynthetic capacity, these findings support the idea that Maf1 regulates the transformation state of cells...
  18. ncbi Novel small-molecule inhibitors of RNA polymerase III
    Liping Wu
    Millennium Pharmaceuticals, Inc, Cambridge, Massachusetts. Pfizer Global Research and Development, Sandwich, United Kingdom
    Eukaryot Cell 2:256-64. 2003
    ..The identification of these inhibitors demonstrates that RNA Pol III can be targeted by small synthetic molecules...
  19. ncbi A universal nomenclature for subunits of the RNA polymerase III transcription initiation factor TFIIIB
    Ian M Willis
    Genes Dev 16:1337-8. 2002
  20. ncbi Multiple kinases and system robustness: a link between Cdc37 and genome integrity
    Avrom J Caplan
    Cell Cycle 6:3145-7. 2007
    ..A network analysis approach related these machines to a small group of cell cycle checkpoint kinases...

Research Grants22

  1. STRUCTURE/FUNCTION OF POL III TRANSCRIPTION FACTORS
    Ian Willis; Fiscal Year: 2002
    ..In addition, the findings will establish aparadigm for considering the function and regulation of the diverse TPR family of proteins. ..
  2. SIGNALING PATHWAYS & TRANSCRIPTIONAL REGULATION
    Ian Willis; Fiscal Year: 2003
    ..And prevent their energetically costly synthesis at inappropriate times. The long term goal of the proposed application is to elucidate the relationship between cell proliferation and transcription of the protein synthetic machinery. ..
  3. STRUCTURAL & FUNCTION OF POL III TRANSCRIPTION FACTORS
    Ian Willis; Fiscal Year: 2007
    ..The hypotheses developed in course of these studies will be explicitly tested by a combination of in vivo and in vitro assays. ..
  4. Transcriptional Repression by Maf1 in Yeast
    Ian Willis; Fiscal Year: 2009
    ..Our genetic, biochemical and structural studies on on Maf1 will enhance understanding of fundamental cellular processes that are likely to impact cancer biology. ..
  5. STRUCTURE & FUNCTION OF POL III TRANSCRIPTION FACTORS
    Ian Willis; Fiscal Year: 1993
    ..Studies on transcription factor structure- function relationships and the regulation of transcription factor activity will be conducted using these in vitro systems together with in vivo and in vitro mutagenesis strategies...
  6. Transcriptional Repression by Maf1 in Yeast
    Ian Willis; Fiscal Year: 2009
    ..Our genetic, biochemical and structural studies on on Maf1 will enhance understanding of fundamental cellular processes that are likely to impact cancer biology. ..