Jonathan Warner

..Komili et al. (2007) now report that these paralogous genes are not functionally equivalent, suggesting the possible existence of a "ribosome code."..

..Is this due to a lack of imaginative evolution by cells and viruses, or to a lack of imaginative experiments by molecular biologists?..

..This is entrusted to a number of signal transduction pathways that can abruptly induce or silence the ribosomal genes, leading to major implications for the expression of other genes as well...

..Two papers published recently in Molecular Cell provide first looks at the association of these processing factors with the intermediates in ribosome synthesis (Harnpicharnchai et al., 2001; Bassler et al., 2001)...

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..In this issue of Molecular Cell, Kos and Tollervey (2010) report their findings from rapid-labeling kinetics and quantitative analyses to address this issue...

..Both of these topics have recently seen important advances that enlighten how almost 200 proteins cooperate to produce a ribosome and how the cell responds to a malfunction in this process...

..We propose a model in which the CURI complex is a key mediator between the two parallel pathways necessary for ribosome synthesis: the transcription and processing of pre-rRNA and the transcription of ribosomal protein genes...

..Fine-structure chromatin mapping of several ribosomal protein promoters revealed that histones are essentially absent from the Rap1 sites, while Fhl1 and Ifh1 are coincident with each other but distinct from Rap1...

..Homeostatic mechanisms within the cell respond by reducing the transcription of rRNA to match the output of RPs, and by reducing the global transcription of mRNA to match the capacity of the translational apparatus...

..cerevisiae and, en passant, raise interesting questions about the relationship between RP genes and telomeres...

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..Our hope, and expectation, is that understanding the circuits that underlie the regulation of ribosome synthesis will lead to more effective intervention when an imbalance occurs. ..

..We will examine the several parts of both the protein degradation pathway and the RNA degradation pathway to determine which is involved in this important task. We propose a mutant screen to identify key players. ..

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..Finally, we will analyze the mechanism for translational control of ribosomal protein synthesis, with emphasis on testing the hypothesis that a regulatory molecule interacts with the m7G cap and its adjacent sequences...

..REB1 appears to share an unusual DNA binding domain with the oncogene, myb. By targeted mutation of the yeast protein we expect to learn more about the characteristics of such DNA-protein interactions...

..region and not much else? (d) eventually, by sequencing the regions adjacent to the 5' end of the mRNA for several ribosomal protein genes, can we identify a sequence which is responsible for coordinate transcription of the genes? ..

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