Jan Vijg

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. pmc Direct, genome-wide assessment of DNA mutations in single cells
    Michael Gundry
    Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461, USA
    Nucleic Acids Res 40:2032-40. 2012
  2. pmc Age- and temperature-dependent somatic mutation accumulation in Drosophila melanogaster
    Ana Maria Garcia
    Department of Biology, University of Texas at San Antonio, San Antonio, Texas, United States of America
    PLoS Genet 6:e1000950. 2010
  3. doi request reprint Genome instability and aging
    Jan Vijg
    Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461, USA
    Annu Rev Physiol 75:645-68. 2013
  4. pmc Effect of Ames dwarfism and caloric restriction on spontaneous DNA mutation frequency in different mouse tissues
    Ana Maria Garcia
    University of Texas at San Antonio, San Antonio, TX, USA
    Mech Ageing Dev 129:528-33. 2008
  5. pmc Myc-dependent genome instability and lifespan in Drosophila
    Christina Greer
    Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America
    PLoS ONE 8:e74641. 2013
  6. ncbi request reprint Detection and analysis of somatic mutations at a lacZ reporter locus in higher organisms: application to Mus musculus and Drosophila melanogaster
    Ana Maria Garcia
    University of Texas at San Antonio, San Antonio, TX, USA
    Methods Mol Biol 371:267-87. 2007
  7. ncbi request reprint Aging and genome maintenance: lessons from the mouse?
    Paul Hasty
    Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Science 299:1355-9. 2003
  8. ncbi request reprint Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase
    James Mele
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 3900, USA
    Antioxid Redox Signal 8:628-38. 2006
  9. ncbi request reprint Organ-specific increase in mutation accumulation and apoptosis rate in CuZn-superoxide dismutase-deficient mice
    Rita A Busuttil
    Departments of Physiology and Molecular Medicine, University of Texas Health Science Center, San Antonio, 78240, USA
    Cancer Res 65:11271-5. 2005
  10. pmc High preservation of CpG cytosine methylation patterns at imprinted gene loci in liver and brain of aged mice
    Silvia Gravina
    Department of Genetics, Albert Einstein College of Medicine, New York, New York, United States of America
    PLoS ONE 8:e73496. 2013

Collaborators

Detail Information

Publications49

  1. pmc Direct, genome-wide assessment of DNA mutations in single cells
    Michael Gundry
    Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461, USA
    Nucleic Acids Res 40:2032-40. 2012
    ..This method can be applied as a direct measure of exposure to mutagenic agents and for assessing genotypic heterogeneity within tissues or cell populations...
  2. pmc Age- and temperature-dependent somatic mutation accumulation in Drosophila melanogaster
    Ana Maria Garcia
    Department of Biology, University of Texas at San Antonio, San Antonio, Texas, United States of America
    PLoS Genet 6:e1000950. 2010
    ..These results show that somatic mutation loads in short-lived flies are much more severe than in the much longer-lived mice, with the mutation rate in flies proportional to biological rather than chronological aging...
  3. doi request reprint Genome instability and aging
    Jan Vijg
    Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461, USA
    Annu Rev Physiol 75:645-68. 2013
    ....
  4. pmc Effect of Ames dwarfism and caloric restriction on spontaneous DNA mutation frequency in different mouse tissues
    Ana Maria Garcia
    University of Texas at San Antonio, San Antonio, TX, USA
    Mech Ageing Dev 129:528-33. 2008
    ..This could be responsible, at least in part, for the enhanced longevity associated with Ames dwarfism and CR...
  5. pmc Myc-dependent genome instability and lifespan in Drosophila
    Christina Greer
    Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America
    PLoS ONE 8:e74641. 2013
    ..Our data provide the first evidence that Myc may act as a pro-aging factor, possibly through its ability to greatly increase genome instability. ..
  6. ncbi request reprint Detection and analysis of somatic mutations at a lacZ reporter locus in higher organisms: application to Mus musculus and Drosophila melanogaster
    Ana Maria Garcia
    University of Texas at San Antonio, San Antonio, TX, USA
    Methods Mol Biol 371:267-87. 2007
    ..This system is capable of detecting a broad range of mutational events, varying from small mutations in the lacZ reporter gene to large genome rearrangements with one breakpoint in lacZ and the other breakpoint elsewhere in the genome...
  7. ncbi request reprint Aging and genome maintenance: lessons from the mouse?
    Paul Hasty
    Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Science 299:1355-9. 2003
    ..Maintaining genome integrity has emerged as a major factor in longevity and cell viability. Here we discuss the use of mouse models with defects in genome maintenance for understanding the molecular basis of aging in humans...
  8. ncbi request reprint Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase
    James Mele
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 3900, USA
    Antioxid Redox Signal 8:628-38. 2006
    ..MEFs from Tg(CAT) +/o and Tg(SOD1/CAT) +/o were equally as resistant to hydrogen peroxide cytotoxicity. However, there were no significant differences in whole animal survival against either paraquat or gamma-radiation...
  9. ncbi request reprint Organ-specific increase in mutation accumulation and apoptosis rate in CuZn-superoxide dismutase-deficient mice
    Rita A Busuttil
    Departments of Physiology and Molecular Medicine, University of Texas Health Science Center, San Antonio, 78240, USA
    Cancer Res 65:11271-5. 2005
    ..5-fold was found not earlier than at 6 months. No increased mutation accumulation was observed in brain or spleen. These results support the hypothesis, that oxidative stress is an important causal factor of cancer in mammals...
  10. pmc High preservation of CpG cytosine methylation patterns at imprinted gene loci in liver and brain of aged mice
    Silvia Gravina
    Department of Genetics, Albert Einstein College of Medicine, New York, New York, United States of America
    PLoS ONE 8:e73496. 2013
    ....
  11. ncbi request reprint Mutant frequencies and spectra depend on growth state and passage number in cells cultured from transgenic lacZ-plasmid reporter mice
    Rita A Busuttil
    Sam and Ann Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center, STCBM Building, Suite 2 200, 15355 Lambda, San Antonio, TX 78245, USA
    DNA Repair (Amst) 5:52-60. 2006
    ..In long-term cultures, the locus is less suitable for studying induced mutations owing to the instability of the cell population...
  12. ncbi request reprint Maintaining genetic integrity in aging: a zero sum game
    Yousin Suh
    Department of Molecular Medicine and Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center, San Antonio, Texas 78425, USA
    Antioxid Redox Signal 8:559-71. 2006
    ..Prospects to reset the clock in this zero sum game between survival and the maintenance of phenotypic integrity will be discussed...
  13. ncbi request reprint Aging and genome maintenance
    Jan Vijg
    University of Texas Health Science Center, STCBM, 15355 Lambda Drive, Suite 2 200, San Antonio, TX 78245, USA
    Ann N Y Acad Sci 1055:35-47. 2005
    ..To directly address this question we demonstrate that it is now possible to analyze single cells, isolated from old and young tissues, for specific alterations in gene expression...
  14. pmc Ku80 deletion suppresses spontaneous tumors and induces a p53-mediated DNA damage response
    Valerie B Holcomb
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245 3207, USA
    Cancer Res 68:9497-502. 2008
    ..Thus, contrary to its assumed role as a caretaker tumor suppressor, Ku80 facilitates tumor growth most likely by dampening baseline cellular DNA damage responses...
  15. ncbi request reprint Catalase transgenic mice: characterization and sensitivity to oxidative stress
    Xinlian Chen
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
    Arch Biochem Biophys 422:197-210. 2004
    ..In addition, the Tg(CAT)(+/+) animals were more sensitive to gamma-irradiation...
  16. pmc Loss of the bloom syndrome helicase increases DNA ligase 4-independent genome rearrangements and tumorigenesis in aging Drosophila
    Ana Maria Garcia
    Department of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
    Genome Biol 12:R121. 2011
    ..To address this, we used a lacZ reporter system in wild-type and several mutant strains of Drosophila melanogaster to analyze mechanisms of mutagenesis throughout their lifespan...
  17. pmc A model system for analyzing somatic mutations in Drosophila melanogaster
    Ana Maria Garcia
    University of Texas at San Antonio, San Antonio, Texas 78249, USA
    Nat Methods 4:401-3. 2007
    ..The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements...
  18. pmc DNA damage in normally and prematurely aged mice
    Alexander Y Maslov
    Department of Genetics, Albert Einstein College of Medicine, New York, NY 10461, USA
    Aging Cell 12:467-77. 2013
    ..This suggests that factors other than DNA damage per se, for example, cellular responses to DNA damage, are responsible for the aging phenotype in mice...
  19. ncbi request reprint Age-related mutation accumulation at a lacZ reporter locus in normal and tumor tissues of Trp53-deficient mice
    Heidi Giese
    Department of Physiology, Sam and Ann Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center, Texas Research Park, 15355 Lambda Drive, Rm 2 200, San Antonio, TX 78245, USA
    Mutat Res 514:153-63. 2002
    ..The accelerated age-related accumulation of mutations in normal spleen and liver could be explained by the defect in apoptosis, which would prevent severely damaged cells from being eliminated...
  20. pmc Genome dynamics in aging mice
    Martijn E T Dollé
    Sam and Ann Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center, San Antonio, Texas 78245, USA
    Genome Res 12:1732-8. 2002
    ..These results lead us to postulate potential mechanisms for the origin of large genome rearrangements in mouse tissues and to predict their possible impact as a potential cause of aging...
  21. pmc Measuring genome instability in aging - a mini-review
    Wenge Li
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Gerontology 58:129-38. 2012
    ..A review of the current methods of mutation detection is timely in view of the lack of insight as to the magnitude of somatic mutation accumulation, the types of mutations that accumulate, and their functional consequences...
  22. doi request reprint A dual-activation, adenoviral-based system for the controlled induction of DNA double-strand breaks by the restriction endonuclease SacI
    Alexander Y Maslov
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biotechniques 47:847-54. 2009
    ..This system should be useful for studying the processing of randomly induced DSBs and their effects on cell fate, without the side effects normally associated with radiation or chemical treatment...
  23. ncbi request reprint Accelerating aging by mouse reverse genetics: a rational approach to understanding longevity
    Paul Hasty
    Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Aging Cell 3:55-65. 2004
    ..This may be in keeping with the recent discovery of a possible 'universal survival' pathway that improves antioxidant defence and genome maintenance and simultaneously extends lifespan in the mouse and several invertebrate species...
  24. pmc Homeostatic imbalance between apoptosis and cell renewal in the liver of premature aging Xpd mice
    Jung Yoon Park
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, United States of America
    PLoS ONE 3:e2346. 2008
    ..These results support a general model for premature aging in DNA repair deficient mice based on cellular responses to DNA damage that impair normal tissue homeostasis...
  25. ncbi request reprint Oxygen accelerates the accumulation of mutations during the senescence and immortalization of murine cells in culture
    Rita A Busuttil
    Sam and Ann Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center, STCBM, San Antonio, TX 78245, USA
    Aging Cell 2:287-94. 2003
    ..These findings demonstrate for the first time the impact of oxidative stress on the genomic integrity of murine cells during senescence and immortalization...
  26. pmc RAD51 mutants cause replication defects and chromosomal instability
    Tae Moon Kim
    Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas, USA
    Mol Cell Biol 32:3663-80. 2012
    ..We found very low levels of mutant protein present at these sites compared to normal protein, suggesting that low levels of mutant protein were sufficient for disruption of RAD51 activity and generation of chromosomal rearrangements...
  27. ncbi request reprint Rebuttal to Miller: 'Accelerated aging': a primrose path to insight?'
    Paul Hasty
    Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Aging Cell 3:67-9. 2004
  28. ncbi request reprint A strategy for the ubiquitous overexpression of human catalase and CuZn superoxide dismutase genes in transgenic mice
    Xinlian Chen
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
    Mech Ageing Dev 124:219-27. 2003
    ..In addition, the expression of catalase closely reflects the tissue specific pattern found in the endogenous gene. These transgenic mice will be useful in studying the role of oxidative stress/damage in aging and age-related pathologies...
  29. ncbi request reprint Impact of genome instability on transcription regulation of aging and senescence
    Jan Vijg
    University of Texas Health Science Center, STCBM Building, Suite 2 200, 15355 Lambda, San Antonio, TX 78245, USA
    Mech Ageing Dev 125:747-53. 2004
    ..To directly address this question, we demonstrate that it is now possible to analyze single cells, isolated from old and young tissues, for specific alterations in gene expression...
  30. ncbi request reprint Genetics of longevity and aging
    Jan Vijg
    University of Texas Health Science Center, San Antonio, Texas 78245, USA
    Annu Rev Med 56:193-212. 2005
    ..This should eventually unravel the genetic factors that contribute to each particular aging phenotype...
  31. doi request reprint Aging: a sirtuin shake-up?
    Jan Vijg
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell 135:797-8. 2008
    ..In this issue, Oberdoerffer et al. (2008) report that SIRT1, the mammalian ortholog of Sir2, is involved in DNA damage-induced chromatin reorganization, which promotes genome stability in mammalian cells...
  32. doi request reprint Epigenetic factors in aging and longevity
    Silvia Gravina
    Department of Genetics, Albert Einstein College of Medicine, 1301 Morris Park Ave, Bronx, New York, NY 10461, USA
    Pflugers Arch 459:247-58. 2010
    ..Increased understanding of those aging-related processes that are driven by epigenetic mechanisms will allow for the development of novel epigenetic-based diagnostic, preventive, and therapeutic strategies for age-related diseases...
  33. doi request reprint Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing
    Wilber Quispe-Tintaya
    Albert Einstein College of Medicine, Department of Genetics, New York, NY, USA
    Biotechniques 55:133-6. 2013
    ..The percentage of sequencing reads aligned to mtDNA was about 22% for non-amplified samples and greater than 99% for samples subjected to 10 cycles of long-range-PCR with mtDNA specific primers. ..
  34. ncbi request reprint Functional genomics of ageing
    Jan Vijg
    Sam and Ann Barshop Center for Longevity and Ageing Studies, University of Texas Health Science Center, 15355 Lambda Drive, STCBM 2 200, San Antonio, TX 78245, USA
    Mech Ageing Dev 124:3-8. 2003
    ..With the emergence of functional genomics, we finally have the opportunity to study ageing in a comprehensive manner, as a function of the dynamic network of genes that determines the physiology of an individual organism over time...
  35. ncbi request reprint Aging. Genomic priorities in aging
    Paul Hasty
    Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245, USA
    Science 296:1250-1. 2002
  36. pmc Mutational fingerprints of aging
    Martijn E T Dollé
    Sam and Ann Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center, 15355 Lambda Drive, STCBM 2 200, San Antonio, TX 78245, USA
    Nucleic Acids Res 30:545-9. 2002
    ..Rather, differences in organ function, possibly in association with replicative history, may explain the divergence in mutation spectra during aging...
  37. doi request reprint Genome instability, cancer and aging
    Alexander Y Maslov
    Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochim Biophys Acta 1790:963-9. 2009
    ..Here, we review age-related changes in the mammalian genome and their possible functional consequences, with special emphasis on genome instability in stem/progenitor cells...
  38. pmc Chromosome-specific accumulation of aneuploidy in the aging mouse brain
    Francesca Faggioli
    Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY 10461, USA
    Hum Mol Genet 21:5246-53. 2012
    ..Such high levels of genome instability could well be a factor in age-related neurodegeneration...
  39. ncbi request reprint Transcripts of aging
    Jan Vijg
    Department of Physiology and Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center, STCBM Building, 15355 Lambda Drive, Suite 2 200, San Antonio, TX 78245, USA
    Trends Genet 20:221-4. 2004
    ..In this article, we discuss the implications of this work for our understanding of the molecular basis of aging and the increasingly important role of microarrays for unraveling the functional pathways underlying the aging phenotype...
  40. pmc Direct mutation analysis by high-throughput sequencing: from germline to low-abundant, somatic variants
    Michael Gundry
    Albert Einstein College of Medicine, Department of Genetics, New York, NY 10461, United States
    Mutat Res 729:1-15. 2012
    ..Some possible approaches to gain access to low-abundance mutations are discussed, with a brief overview of new sequencing platforms that are currently waiting in the wings to advance this exploding field even further...
  41. ncbi request reprint SNP discovery in associating genetic variation with human disease phenotypes
    Yousin Suh
    Department of Physiology, Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mutat Res 573:41-53. 2005
    ....
  42. ncbi request reprint Large genome rearrangements as a primary cause of aging
    Jan Vijg
    Department of Physiology, Sam and Ann Barshop Center for Aging and Longevity Research, STCBM, Room 2 200, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mech Ageing Dev 123:907-15. 2002
    ....
  43. pmc Comprehensive microRNA profiling in B-cells of human centenarians by massively parallel sequencing
    Saurabh Gombar
    Department of Systems and Computational Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    BMC Genomics 13:353. 2012
    ..Despite their demonstrated roles in age-associated pathologies, little is known about the role of miRNAs in human aging and longevity...
  44. doi request reprint Four-color FISH for the detection of low-level aneuploidy in interphase cells
    Francesca Faggioli
    Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Michael F Price Center, 1301 Morris Park Avenue, Bronx, NY, 10461, USA
    Methods Mol Biol 1136:291-305. 2014
    ..It greatly reduces the enumeration of false-positive signals that are challenging in the enumeration of ploidy changes (particularly if these are complex and/or involve a significant increase of chromosome number). ..
  45. pmc Deletion of individual Ku subunits in mice causes an NHEJ-independent phenotype potentially by altering apurinic/apyrimidinic site repair
    Yong Jun Choi
    Department of Molecular Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America
    PLoS ONE 9:e86358. 2014
    ..In addition, free Ku70 and free Ku80 bound to AP sites and in the case of Ku70 inhibited APE1 activity. These observations support a novel role for free Ku70 and free Ku80 in altering BER. ..
  46. ncbi request reprint Aging and p53: getting it straight. A commentary on a recent paper by Gentry and Venkatachalam
    Jan Vijg
    Department of Physiology, University of Texas Health Science Center, San Antonio, 78245, USA
    Aging Cell 4:331-3. 2005
  47. pmc Chromosomal aneuploidy in the aging brain
    Francesca Faggioli
    Department of Genetics, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA
    Mech Ageing Dev 132:429-36. 2011
    ..Here, we will review chromosomal aneuploidy in the aging brain, its possible causes, its consequences for cellular homeostasis and its possible link to functional decline and neuropathies...
  48. ncbi request reprint Searching for genetic determinants of human aging and longevity: opportunities and challenges
    Jan Vijg
    Sam and Ann Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center, 15355 Lambda Drive, San Antonio, TX 78245, USA
    Mech Ageing Dev 123:195-205. 2002
    ..Here, we describe the use of one such method, two-dimensional gene scanning (TDGS), for screening populations of centenarians and controls for polymorphic variation in the large BRCA1 breast cancer susceptibility gene...
  49. pmc Genome-wide quantitative analysis of DNA methylation from bisulfite sequencing data
    Kemal Akman
    Tresch Group, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany, AG Haaf, Institute of Human Genetics, Julius Maximilians University, 97070 Wuerzburg, Germany and Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Bioinformatics 30:1933-4. 2014
    ..Availability and implementation: BEAT is freely available as part of Bioconductor at www.bioconductor.org/packages/devel/bioc/html/BEAT.html. The package is distributed under the GNU Lesser General Public License 3.0...