Robert Singer

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. pmc A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons
    Wei Gu
    Department of Anatomy, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 156:41-51. 2002
  2. pmc Two ZBP1 KH domains facilitate beta-actin mRNA localization, granule formation, and cytoskeletal attachment
    Kim L Farina
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 160:77-87. 2003
  3. ncbi request reprint Imaging of gene expression in living cells and tissues
    Robert H Singer
    Albert Einstein College of Medicine, Department of Anatomy and Structural Biology and Biochemistry, Biophotonics Center, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    J Biomed Opt 10:051406. 2005
  4. ncbi request reprint Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 62:6278-88. 2002
  5. ncbi request reprint Dynamics of single mRNPs in nuclei of living cells
    Yaron Shav-Tal
    Departments of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 304:1797-800. 2004
  6. ncbi request reprint ZBP1 enhances cell polarity and reduces chemotaxis
    Kyle Lapidus
    Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461 1975, USA
    J Cell Sci 120:3173-8. 2007
  7. ncbi request reprint Measurement of barbed ends, actin polymerization, and motility in live carcinoma cells after growth factor stimulation
    Mike Lorenz
    Albert Einstein College of Medicine, Department of Anatomy and Structural Biology, Bronx, NY 10461, USA
    Cell Motil Cytoskeleton 57:207-17. 2004
  8. pmc ZBP2 facilitates binding of ZBP1 to beta-actin mRNA during transcription
    Feng Pan
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Mol Cell Biol 27:8340-51. 2007
  9. ncbi request reprint Localization of a beta-actin messenger ribonucleoprotein complex with zipcode-binding protein modulates the density of dendritic filopodia and filopodial synapses
    Taesun Eom
    Department of Neuroscience, Rose F Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 23:10433-44. 2003
  10. ncbi request reprint Activity-dependent trafficking and dynamic localization of zipcode binding protein 1 and beta-actin mRNA in dendrites and spines of hippocampal neurons
    Dhanrajan M Tiruchinapalli
    Department of Neuroscience, Rose F Kennedy Center for Research in Mental Retardation and Human Development, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 23:3251-61. 2003

Detail Information

Publications74

  1. pmc A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons
    Wei Gu
    Department of Anatomy, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 156:41-51. 2002
    ..These data suggest that ZBP2, although predominantly a nuclear protein, has a role in the cytoplasmic localization of beta-actin mRNA...
  2. pmc Two ZBP1 KH domains facilitate beta-actin mRNA localization, granule formation, and cytoskeletal attachment
    Kim L Farina
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 160:77-87. 2003
    ....
  3. ncbi request reprint Imaging of gene expression in living cells and tissues
    Robert H Singer
    Albert Einstein College of Medicine, Department of Anatomy and Structural Biology and Biochemistry, Biophotonics Center, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    J Biomed Opt 10:051406. 2005
    ....
  4. ncbi request reprint Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 62:6278-88. 2002
    ..We conclude that aligning cell behavior in vivo with patterns of gene expression can lead to new insights into the microenvironment of carcinoma cells in the primary tumor and the molecular mechanisms behind cell behavior...
  5. ncbi request reprint Dynamics of single mRNPs in nuclei of living cells
    Yaron Shav-Tal
    Departments of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 304:1797-800. 2004
    ..This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model...
  6. ncbi request reprint ZBP1 enhances cell polarity and reduces chemotaxis
    Kyle Lapidus
    Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461 1975, USA
    J Cell Sci 120:3173-8. 2007
    ..We found that ZBP1 expression actually reduced tumor cell motility and chemotaxis, presumably mediating their decreased metastatic potential by reducing their ability to respond to signals necessary for invasion...
  7. ncbi request reprint Measurement of barbed ends, actin polymerization, and motility in live carcinoma cells after growth factor stimulation
    Mike Lorenz
    Albert Einstein College of Medicine, Department of Anatomy and Structural Biology, Bronx, NY 10461, USA
    Cell Motil Cytoskeleton 57:207-17. 2004
    ..This assay has a high potential for applications where rapid changes in the dynamic filament population are to be measured...
  8. pmc ZBP2 facilitates binding of ZBP1 to beta-actin mRNA during transcription
    Feng Pan
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Mol Cell Biol 27:8340-51. 2007
    ..ZBP1 then associates with the RNA throughout the nuclear export and cytoplasmic localization process...
  9. ncbi request reprint Localization of a beta-actin messenger ribonucleoprotein complex with zipcode-binding protein modulates the density of dendritic filopodia and filopodial synapses
    Taesun Eom
    Department of Neuroscience, Rose F Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 23:10433-44. 2003
    ..These results reveal a novel function for an mRNA localization element and its binding protein in the regulation of dendritic morphology and synaptic growth via dendritic filopodia...
  10. ncbi request reprint Activity-dependent trafficking and dynamic localization of zipcode binding protein 1 and beta-actin mRNA in dendrites and spines of hippocampal neurons
    Dhanrajan M Tiruchinapalli
    Department of Neuroscience, Rose F Kennedy Center for Research in Mental Retardation and Human Development, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 23:3251-61. 2003
    ..The activity-dependent trafficking of ZBP1 and its dynamic movements within dendritic spines provide new evidence to implicate RNA binding proteins as regulators of mRNA transport to activated synapses in response to synaptic activity...
  11. ncbi request reprint The great escape: when cancer cells hijack the genes for chemotaxis and motility
    John Condeelis
    Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461 1975, USA
    Annu Rev Cell Dev Biol 21:695-718. 2005
    ..We discuss how these new insights may lead to a better understanding of the molecular basis of the invasive behavior of tumor cells in vivo, which may result in new strategies for the diagnosis and treatment of metastasis...
  12. pmc Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)
    Olivier Loudig
    Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, NY, USA
    Nucleic Acids Res 35:e94. 2007
    ..Our results indicate that CT-RT allows microarray profiling of severely degraded RNA that could not be analyzed by previous methods...
  13. ncbi request reprint Single-cell gene expression profiling
    Jeffrey M Levsky
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Science 297:836-40. 2002
    ..Using the nucleus as the substrate for parallel gene analysis, we provide a platform for the fusion of genomics and cell biology: "cellular genomics."..
  14. pmc Visualization of mRNA translation in living cells
    Alexis J Rodriguez
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 175:67-76. 2006
    ..Additionally, localization of beta-actin mRNA and translation activity was enhanced at cell contacts and facilitated the formation of intercellular junctions...
  15. ncbi request reprint Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 64:8585-94. 2004
    ..ZBP1 reexpression also inhibited metastasis from tumors. These experiments support the involvement in metastasis of the pathways identified in invasive cells, which are regulated by ZBP1...
  16. pmc Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibition
    Yaron Shav-Tal
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100 Israel
    Mol Biol Cell 16:2395-413. 2005
    ....
  17. ncbi request reprint Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1
    Stefan Huttelmaier
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nature 438:512-5. 2005
    ..These sequential events provide both temporal and spatial control over beta-actin mRNA translation, which is important for cell migration and neurite outgrowth...
  18. ncbi request reprint Imaging sites of N-wasp activity in lamellipodia and invadopodia of carcinoma cells
    Mike Lorenz
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Curr Biol 14:697-703. 2004
    ..This is the first time that the activity of full-length N-WASP has been visualized in vivo, and this has lead to new insights for N-WASP function...
  19. ncbi request reprint Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization
    Yuri Oleynikov
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Curr Biol 13:199-207. 2003
    ..The ZBP1 protein contains four KH RNA binding domains and a classic RBD RNA binding domain. It also contains a putative nuclear import and export sequence, suggesting a nuclear phase in this protein's function...
  20. pmc Blocking beta-catenin binding to the ZBP1 promoter represses ZBP1 expression, leading to increased proliferation and migration of metastatic breast-cancer cells
    Wei Gu
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Cell Sci 122:1895-905. 2009
    ..These data suggest that repression of ZBP1 deregulates its associated mRNAs, leading to the phenotypic changes of breast cancers...
  21. ncbi request reprint Single mRNA molecules demonstrate probabilistic movement in living mammalian cells
    Dahlene Fusco
    Departments of Anatomy, Albert Einstein College of Medicine, 10461, Bronx, NY, USA
    Curr Biol 13:161-7. 2003
    ..Individual mRNA molecules switched frequently among these movements, suggesting that mRNAs undergo continuous cycles of anchoring, diffusion, and active transport...
  22. pmc Feedback regulation between zipcode binding protein 1 and beta-catenin mRNAs in breast cancer cells
    Wei Gu
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Mol Cell Biol 28:4963-74. 2008
    ..beta-Catenin mediates the transcription of the ZBP1 gene, while ZBP1 promotes the stability of beta-catenin mRNA...
  23. ncbi request reprint The spatial order of transcription in mammalian cells
    Jeffrey M Levsky
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    J Cell Biochem 102:609-17. 2007
    ..The approach has sufficient spatial resolution to be applied to the detection of chromosomal translocations or the identification of cancer cells...
  24. pmc Nuclear microenvironment in cancer diagnosis and treatment
    Rossanna C Pezo
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, USA
    J Cell Biochem 104:1953-63. 2008
    ....
  25. ncbi request reprint Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization
    Honglai L Zhang
    Department of Neuroscience, Rose F Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 23:6627-37. 2003
    ..Such observations provide motivation to investigate possible transport defects or inefficiency of SMN associated RNPs in motor neuron axons in SMA...
  26. ncbi request reprint In vivo dynamics of RNA polymerase II transcription
    Xavier Darzacq
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Struct Mol Biol 14:796-806. 2007
    ..Our systems approach, quantifying both polymerase and mRNA kinetics on a defined DNA template in vivo with high temporal resolution, opens new avenues for studying regulation of transcriptional processes in vivo...
  27. pmc Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells
    Sandra Maier
    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10467, USA
    J Cell Physiol 218:638-42. 2009
    ....
  28. pmc ZBP1 recognition of beta-actin zipcode induces RNA looping
    Jeffrey A Chao
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genes Dev 24:148-58. 2010
    ..The RNA looping induced by ZBP1 binding provides a mechanism for specific recognition and may facilitate the assembly of post-transcriptional regulatory complexes by remodeling the bound transcript...
  29. ncbi request reprint Developmental timing in Dictyostelium is regulated by the Set1 histone methyltransferase
    Jonathan R Chubb
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, The Bronx, NY 10461, USA
    Dev Biol 292:519-32. 2006
    ..Microarray analysis of the set1 mutants reveals genomic clustering of mis-expressed genes, suggesting a requirement for Set1 in the regulation of chromatin-mediated events at gene clusters...
  30. pmc Dynamic association and localization of human H/ACA RNP proteins
    Nupur Kittur
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, New York 10461, USA
    RNA 12:2057-62. 2006
    ....
  31. ncbi request reprint Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons
    Honglai Zhang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 26:8622-32. 2006
    ..These findings provide new insight into the molecular composition of distinct SMN multiprotein complexes in neurons and motivation to investigate deficiencies of localized RNPs in SMA...
  32. pmc A direct role for FMRP in activity-dependent dendritic mRNA transport links filopodial-spine morphogenesis to fragile X syndrome
    Jason B Dictenberg
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Dev Cell 14:926-39. 2008
    ..These findings highlight a mechanism for stimulus-induced dendritic mRNA transport and link its impairment in a mouse model of FXS to altered developmental morphologic plasticity...
  33. pmc Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation
    Yingfeng Deng
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genes Dev 22:1037-50. 2008
    ....
  34. pmc The cytoplasmic fate of an mRNP is determined cotranscriptionally: exception or rule?
    Tatjana Trcek
    Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genes Dev 24:1827-31. 2010
    ..This demonstrates yet another case where the mRNA's cytoplasmic fate is determined by the RNAPII complex...
  35. pmc Interactions of elongation factor 1alpha with F-actin and beta-actin mRNA: implications for anchoring mRNA in cell protrusions
    Gang Liu
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, New York, New York 10461, USA
    Mol Biol Cell 13:579-92. 2002
    ..Taken together, these results implicate EF1alpha in the anchoring of beta-actin mRNA to the protrusion in crawling cells...
  36. ncbi request reprint How and why does beta-actin mRNA target?
    John Condeelis
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Biol Cell 97:97-110. 2005
    ..Hence the physiological consequences of beta-actin mRNA delocalization affect the stability of the cell phenotype...
  37. pmc A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization
    Wei Gu
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genes Dev 18:1452-65. 2004
    ..We propose that Puf6p is a protein that functions in the translational control of ASH1 mRNA, and this translational inhibition is necessary before localization can proceed...
  38. ncbi request reprint Imaging mRNA movement from transcription sites to translation sites
    Alexis J Rodriguez
    Gruss Lipper Biophotonics Center, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States
    Semin Cell Dev Biol 18:202-8. 2007
    ..We review advances in these methods with the goal of identifying the particular strengths and weaknesses of the various approaches in their ability to follow the movements of mRNAs from transcription sites to translation sites...
  39. ncbi request reprint QNQKE targeting motif for the SMN-Gemin multiprotein complexin neurons
    Honglai Zhang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA
    J Neurosci Res 85:2657-67. 2007
    ..These findings provide further evidence in support of a neuronal function for SMN and motivation to investigate for impaired assembly and/or localization of mRNP complexes as an underlying cause of SMA...
  40. ncbi request reprint Imaging gene expression in single living cells
    Yaron Shav-Tal
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nat Rev Mol Cell Biol 5:855-61. 2004
    ..This allows the visualization and quantification of gene expression and intracellular trafficking events of the studied molecules and the associated enzymatic processes in individual cells, in real time...
  41. pmc Single-cell transcription site activation predicts chemotherapy response in human colorectal tumors
    Rossanna C Pezo
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Albert Einstein Cancer Center, Bronx, New York 10461, USA
    Cancer Res 68:4977-82. 2008
    ..This approach provides a novel ability to identify and characterize unique minor cell populations in the tumor that may exhibit relative resistance to chemotherapy...
  42. ncbi request reprint Imaging of single mRNAs in the cytoplasm of living cells
    Dahlene Fusco
    Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Prog Mol Subcell Biol 35:135-50. 2004
  43. ncbi request reprint Asymmetric sorting of ash1p in yeast results from inhibition of translation by localization elements in the mRNA
    Pascal Chartrand
    Department of Anatomy and Structural Biology, Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Cell 10:1319-30. 2002
    ..In support of this, Ash1p asymmetry could be rescued by slowing its translation...
  44. ncbi request reprint RNA localization: visualization in real-time
    Robert H Singer
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Curr Biol 13:R673-5. 2003
    ..RNA localization during development is required for proper sorting of developmental determinants. Direct visualization of endogenous transcribed RNA is now possible and should provide new insights into how this process occurs...
  45. ncbi request reprint Fluorescence in situ hybridization: past, present and future
    Jeffrey M Levsky
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Cell Sci 116:2833-8. 2003
    ..In the future, this technique is likely to have significant further impact on live-cell imaging and on medical diagnostics...
  46. pmc Single-RNA counting reveals alternative modes of gene expression in yeast
    Daniel Zenklusen
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Struct Mol Biol 15:1263-71. 2008
    ..These data directly demonstrate the existence of multiple expression modes used to modulate the transcriptome...
  47. ncbi request reprint The nuclear connection in RNA transport and localization
    Kim L Farina
    Dept of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
    Trends Cell Biol 12:466-72. 2002
    ..These proteins might link nuclear events, such as RNA splicing, with the subsequent cytoplasmic localization of specific transcripts...
  48. pmc Cell biology of mRNA decay
    DAVID GRUNWALD
    Albert Einstein College of Medicine, Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Bronx, Albert Einstein College of Medicine, New York, USA
    Methods Enzymol 448:553-77. 2008
    ..For this reason, understanding of spatial relationships between mRNA and mRNA turnover factors should enrich our understanding of this process...
  49. pmc Single mRNA tracking in live cells
    Hye Yoon Park
    Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, USA
    Methods Enzymol 472:387-406. 2010
    ..This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection...
  50. ncbi request reprint Dynamics of transcription and mRNA export
    Xavier Darzacq
    Department of Anatomy, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Curr Opin Cell Biol 17:332-9. 2005
    ..By contrast, an understanding of the dynamics of an individual gene's expression in real time is just beginning to emerge following recent technological developments...
  51. pmc Promotion of importin alpha-mediated nuclear import by the phosphorylation-dependent binding of cargo protein to 14-3-3
    Christian Faul
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 169:415-24. 2005
    ..They provide a molecular explanation for the phosphorylation-dependent nuclear import of nuclear localization signal-containing cargo proteins...
  52. pmc A single molecule view of gene expression
    Daniel R Larson
    Department of Anatomy and Structural Biology and The Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Trends Cell Biol 19:630-7. 2009
    ..Thus, the close coupling between imaging and mathematical theory has established single-cell analysis as an essential branch of systems biology...
  53. ncbi request reprint Novel detection and differential utilization of a c-myc transcriptional block in colon cancer chemoprevention
    Andrew J Wilson
    Department of Oncology, Albert Einstein Cancer Center, Montefiore Medical Center, Bronx, New York 10467, USA
    Cancer Res 62:6006-10. 2002
    ..We have described a novel approach for evaluating the effects of chemopreventive agents on the expression of a gene critical in colonic tumorigenesis...
  54. ncbi request reprint Pathways for mRNA localization in the cytoplasm
    Kevin Czaplinski
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461718 430 8646, USA
    Trends Biochem Sci 31:687-93. 2006
    ..Different mechanisms have been identified that enable localized mRNAs to target different subcellular compartments, and recent advances in understanding these pathways is reviewed here...
  55. pmc Mechanisms and cellular roles of local protein synthesis in mammalian cells
    Alexis J Rodriguez
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States
    Curr Opin Cell Biol 20:144-9. 2008
    ..Identifying regulatory sites on transport factors as well as novel physiological functions for well-known translation factors has provided significant advances in how spatially controlled translation impacts cell function...
  56. ncbi request reprint Transcriptional pulsing of a developmental gene
    Jonathan R Chubb
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, The Bronx, New York 10461, USA
    Curr Biol 16:1018-25. 2006
    ..This study represents the first direct visualization of transcriptional pulsing in eukaryotes. Discontinuity of transcription may allow greater flexibility in the gene-expression decisions of a cell...
  57. pmc Altered dynamics of intestinal cell maturation in Apc1638N/+ mice
    Donghai Wang
    Department of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Cancer Res 70:5348-57. 2010
    ....
  58. ncbi request reprint Visualization of single molecules of mRNA in situ
    Andrea M Femino
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Methods Enzymol 361:245-304. 2003
  59. pmc Structural basis for the coevolution of a viral RNA-protein complex
    Jeffrey A Chao
    Department of Anatomy, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, New York 10461, USA
    Nat Struct Mol Biol 15:103-5. 2008
    ....
  60. ncbi request reprint Role of the parafusin orthologue, PRP1, in microneme exocytosis and cell invasion in Toxoplasma gondii
    Steen H Matthiesen
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cell Microbiol 5:613-24. 2003
    ..These studies suggest that PRP1 associated with the secretory vesicle scaffold serves an important role in Ca2+-regulated exocytosis and cell invasion...
  61. ncbi request reprint Yeast mitochondrial transcriptomics
    Mathilde Garcia
    Laboratoire de Génétique Moléculaire CNRS ENS, Paris, France
    Methods Mol Biol 372:505-28. 2007
    ..Finally, a fluorescence microscopy approach on a single living cell is described to visualize the in vivo localization of mRNAs involved in mitochondria biogenesis...
  62. pmc Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts
    Lisa A Mingle
    Center for Cell Biology and Cancer Research, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208, USA
    J Cell Sci 118:2425-33. 2005
    ..In addition, myosin inhibitors significantly inhibit Arp2 mRNA localization in chicken embryo fibroblasts, suggesting a myosin motor dependent mechanism for Arp2/3-complex mRNA localization...
  63. pmc Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells
    Xavier Darzacq
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine Bronx, NY 10461, USA
    J Cell Biol 173:207-18. 2006
    ..Our analyses indicate that NAF1 binds NAP57 and escorts it to the nascent H/ACA RNA and that GAR1 then replaces NAF1 to yield mature H/ACA RNPs in Cajal bodies and nucleoli...
  64. ncbi request reprint A peptide motif in Raver1 mediates splicing repression by interaction with the PTB RRM2 domain
    Alexis P Rideau
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA, UK
    Nat Struct Mol Biol 13:839-48. 2006
    ..The requirement for the PTB-Raver1 interaction that we have characterized may serve to bring the additional repressor regions of both proteins into a configuration that allows them to synergistically effect exon skipping...
  65. pmc Gene expression profiling in single cells within tissue
    Paola Capodieci
    Aureon Laboratories, 28 Wells Avenue, Yonkers, New York 10701, USA
    Nat Methods 2:663-5. 2005
    ..This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures...
  66. pmc YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA
    Shuyun Dong
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mol Cell 25:559-73. 2007
    ....
  67. ncbi request reprint From silencing to gene expression: real-time analysis in single cells
    Susan M Janicki
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 116:683-98. 2004
    ..Using this system, we are able to correlate changes in chromatin structure with the progression of transcriptional activation allowing us to obtain a real-time integrative view of gene expression...
  68. pmc The PTB interacting protein raver1 regulates alpha-tropomyosin alternative splicing
    Natalia Gromak
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    EMBO J 22:6356-64. 2003
    ..This suggests a novel mechanism for PTB-mediated splicing repression involving recruitment of raver1 as a potent splicing co-repressor...
  69. pmc Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body
    Wakam Chang
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    RNA 14:491-502. 2008
    ..P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body...
  70. pmc Assembling an intermediate filament network by dynamic cotranslation
    Lynne Chang
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    J Cell Biol 172:747-58. 2006
    ..The results show that the motility and targeting of peripherin mRNPs, their translational control, and the assembly of an IF cytoskeletal system are linked together in a process we have termed dynamic cotranslation...
  71. ncbi request reprint She2p is a novel RNA binding protein with a basic helical hairpin motif
    Dierk Niessing
    Laboratories of Molecular Biophysics and The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Cell 119:491-502. 2004
    ....
  72. pmc Gene expression within a dynamic nuclear landscape
    Yaron Shav-Tal
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel
    EMBO J 25:3469-79. 2006
    ..We also describe the efforts being made towards physiological approaches within the context of living organisms...
  73. pmc The dynamic range of transcription
    Xavier Darzacq
    Ecole Normale Superieure, CNRS UMR 8541, 46 rue d Ulm 75230, Paris Cedex 05, France
    Mol Cell 30:545-6. 2008
    ..In a recent issue of Molecular Cell, Gorski et al. (2008) demonstrate directly that polymerase assembly kinetics regulate Pol I transcription...
  74. pmc ZBP1 regulates mRNA stability during cellular stress
    Nadine Stöhr
    NBL3 Research Group, Zentrum für Angewandte Medizinische und Humanbiologische Forschung, Department of Medicine, Martin Luther University, 06120 Halle, Germany
    J Cell Biol 175:527-34. 2006
    ..These retain mRNAs in SGs and prevent premature decay in PBs. Hence, mRNA-binding proteins are essential for translational adaptation during cellular stress by modulating mRNA turnover...

Research Grants50

  1. PROBES FOR MULTIPLEXING SINGLE RNA MOLECULE DETECTIION IN LIVING CELLS
    Robert H Singer; Fiscal Year: 2010
    ..4. To multiplex the probes with different fluorochromes so that we will be able to detect multiple RNA species in the same cell. Ultimately, the approach can be formatted for use in living tissue. ..
  2. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2000
    ..abstract_text> ..
  3. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2006
    ..Using these approaches, we will have the opportunity to identify where in the pathway the components of localization and regulation of protein expression operate, with high spatial and temporal resolution. ..
  4. MECHANISM OF ACTIN MRNA LOCALIZATION
    Robert Singer; Fiscal Year: 2006
    ..We will investigate the pathway using high-resolution microscopy combined with FRET to visualize the spatial distribution of these protein interactions in real time. ..
  5. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2006
    ....
  6. FISH & CHIPS: SINGLE CELL EXPRESSION OF CANCER GENES
    Robert Singer; Fiscal Year: 2006
    ..The expression patterns obtained from tumor cells in pathological samples will allow a correlation of specific genes with disease progression. ..
  7. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2009
    ....
  8. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2001
    ..abstract_text> ..
  9. LIGHT-ACTIVATED GENE EXPRESSION IN SINGLE CELLS(RMI)
    Robert Singer; Fiscal Year: 2007
    ..3. Intravitally imaging of this expression in a single cell within tumor tissue. ..
  10. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 1999
    ..In the final specific aim, the mechanism of nuclear export will be assessed to determine points in the pathway where rate-limitations may exist. ..
  11. MECHANISM OF ACTIN MRNA LOCALIZATION
    Robert Singer; Fiscal Year: 1999
    ....
  12. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2000
    ..In the final specific aim, the mechanism of nuclear export will be assessed to determine points in the pathway where rate-limitations may exist. ..
  13. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2001
    ..In the final specific aim, the mechanism of nuclear export will be assessed to determine points in the pathway where rate-limitations may exist. ..
  14. LIGHT-ACTIVATED GENE EXPRESSION IN SINGLE CELLS WITHIN TISSUE
    Robert Singer; Fiscal Year: 2009
    ..that controls how and where other proteins are made in the cell. We are particularly interested in proteins that cause cancer, oncogenes, and in determining how these proteins can induce cells to produce tumors. ..
  15. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2007
    ..at the top of each printed page and each continuation page. RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1-2 Description, ..
  16. MECHANISM OF ACTIN MRNA LOCALIZATION
    Robert H Singer; Fiscal Year: 2010
    ..We are investigating how this protein works to localize protein synthesis in specific regions of the cell by using a super-sensitive microscope to watch the process occur in living cells and tissues from genetically engineered mice. ..
  17. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert H Singer; Fiscal Year: 2010
    ....
  18. MECHANISM OF ACTIN MRNA LOCALIZATION
    Robert Singer; Fiscal Year: 2009
    ..We are investigating how this protein works to localize protein synthesis in specific regions of the cell by using a super-sensitive microscope to watch the process occur in living cells and tissues from genetically engineered mice. ..
  19. LIGHT-ACTIVATED GENE EXPRESSION IN SINGLE CELLS WITHIN TISSUE
    Robert H Singer; Fiscal Year: 2010
    ..that controls how and where other proteins are made in the cell. We are particularly interested in proteins that cause cancer, oncogenes, and in determining how these proteins can induce cells to produce tumors. ..
  20. PROBES FOR MULTIPLEXING SINGLE RNA MOLECULE DETECTIION IN LIVING CELLS
    Robert Singer; Fiscal Year: 2009
    ..4. To multiplex the probes with different fluorochromes so that we will be able to detect multiple RNA species in the same cell. Ultimately, the approach can be formatted for use in living tissue. ..
  21. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2007
    ....
  22. PROBES FOR MULTIPLEXING SINGLE RNA MOLECULE DETECTIION IN LIVING CELLS
    Robert Singer; Fiscal Year: 2007
    ..4. To multiplex the probes with different fluorochromes so that we will be able to detect multiple RNA species in the same cell. Ultimately, the approach can be formatted for use in living tissue. ..
  23. MECHANISM OF ACTIN MRNA LOCALIZATION
    Robert Singer; Fiscal Year: 2000
    ....
  24. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2002
    ..abstract_text> ..
  25. RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU
    Robert Singer; Fiscal Year: 2003
    ..Using these approaches, we will have the opportunity to identify where in the pathway the components of localization and regulation of protein expression operate, with high spatial and temporal resolution. ..
  26. MECHANISM OF ACTIN MRNA LOCALIZATION
    Robert Singer; Fiscal Year: 2003
    ..We will investigate the pathway using high-resolution microscopy combined with FRET to visualize the spatial distribution of these protein interactions in real time. ..
  27. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2003
    ..abstract_text> ..
  28. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2004
    ....
  29. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2005
    ....
  30. High Speed Confocal Photomanipulation Microscopyfor use in multi-user facility
    Robert Singer; Fiscal Year: 2007
    ..The high speed microscope described in this proposal will fulfill this need for our research groups. ..
  31. SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS
    Robert Singer; Fiscal Year: 2007
    ....