RICHARD LOPACHIN

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. pmc Synaptosomal toxicity and nucleophilic targets of 4-hydroxy-2-nonenal
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Toxicol Sci 107:171-81. 2009
  2. ncbi request reprint Intraneuronal ion distribution during experimental oxygen/glucose deprivation. Routes of ion flux as targets of neuroprotective strategies
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Ann N Y Acad Sci 890:191-203. 1999
  3. ncbi request reprint Protein adduct formation as a molecular mechanism in neurotoxicity
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467 2490, USA
    Toxicol Sci 86:214-25. 2005
  4. ncbi request reprint Gamma-Diketone central neuropathy: quantitative analyses of cytoskeletal components in myelinated axons of the rat rubrospinal tract
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Moses Research Tower 7, 111 E 2210th St, Bronx, NY 10467 2490, USA
    Neurotoxicology 26:1021-30. 2005
  5. ncbi request reprint Acrylamide inhibits dopamine uptake in rat striatal synaptic vesicles
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Toxicol Sci 89:224-34. 2006
  6. ncbi request reprint Acrylamide neurotoxicity: neurological, morhological and molecular endpoints in animal models
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, 111 E 210th St, Bronx, NY 10467, USA
    Adv Exp Med Biol 561:21-37. 2005
  7. ncbi request reprint Synaptic cysteine sulfhydryl groups as targets of electrophilic neurotoxicants
    Richard M LoPachin
    Department of Anesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York 10467 2490, USA
    Toxicol Sci 94:240-55. 2006
  8. ncbi request reprint Structure-toxicity analysis of type-2 alkenes: in vitro neurotoxicity
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Toxicol Sci 95:136-46. 2007
  9. ncbi request reprint Neurotoxic mechanisms of electrophilic type-2 alkenes: soft soft interactions described by quantum mechanical parameters
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111 E 210th St, Bronx, New York 10467, USA
    Toxicol Sci 98:561-70. 2007
  10. pmc Molecular mechanisms of the conjugated alpha,beta-unsaturated carbonyl derivatives: relevance to neurotoxicity and neurodegenerative diseases
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467 2490, USA
    Toxicol Sci 104:235-49. 2008

Research Grants

  1. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2003
  2. The Nerve Terminal as a Site of Acrylamide Action
    RICHARD LOPACHIN; Fiscal Year: 2006
  3. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2007
  4. The Nerve Terminal as the Site of Action for Type-2 Alkenes
    RICHARD LOPACHIN; Fiscal Year: 2009
  5. NERVE TERMINAL AS A SITE OF ACRYLAMIDE ACTION
    RICHARD LOPACHIN; Fiscal Year: 2002
  6. MOLECULAR MECHANISMS OF HEXACARBON INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 1999
  7. The Nerve Terminal as the Site of Action for Type-2 Alkenes
    RICHARD MICHAEL LOPACHIN; Fiscal Year: 2010
  8. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2009

Detail Information

Publications28

  1. pmc Synaptosomal toxicity and nucleophilic targets of 4-hydroxy-2-nonenal
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Toxicol Sci 107:171-81. 2009
    ..These data suggest that, like other type 2 alkenes, HNE produces nerve terminal toxicity by forming adducts with sulfhydryl thiolates on proteins involved in neurotransmission...
  2. ncbi request reprint Intraneuronal ion distribution during experimental oxygen/glucose deprivation. Routes of ion flux as targets of neuroprotective strategies
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Ann N Y Acad Sci 890:191-203. 1999
    ..These findings provide a foundation for identifying potential pharmacotherapeutic approaches and for discerning corresponding mechanisms of neuroprotection...
  3. ncbi request reprint Protein adduct formation as a molecular mechanism in neurotoxicity
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467 2490, USA
    Toxicol Sci 86:214-25. 2005
    ..This is a challenging but critical objective that will be facilitated by recent advances in proteomic methodologies...
  4. ncbi request reprint Gamma-Diketone central neuropathy: quantitative analyses of cytoskeletal components in myelinated axons of the rat rubrospinal tract
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Moses Research Tower 7, 111 E 2210th St, Bronx, NY 10467 2490, USA
    Neurotoxicology 26:1021-30. 2005
    ..Together, these findings implicate loss of mobile NF proteins as the primary mechanism of axon atrophy...
  5. ncbi request reprint Acrylamide inhibits dopamine uptake in rat striatal synaptic vesicles
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Toxicol Sci 89:224-34. 2006
    ..The resulting disruption of neurotransmitter storage might mediate defective presynaptic release...
  6. ncbi request reprint Acrylamide neurotoxicity: neurological, morhological and molecular endpoints in animal models
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, 111 E 210th St, Bronx, NY 10467, USA
    Adv Exp Med Biol 561:21-37. 2005
    ..In this publication we provide an overview of recent advances in AA research. This includes a discussion of the cumulative nature of AA neurotoxicity and the putative sites and molecular mechanisms of action...
  7. ncbi request reprint Synaptic cysteine sulfhydryl groups as targets of electrophilic neurotoxicants
    Richard M LoPachin
    Department of Anesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York 10467 2490, USA
    Toxicol Sci 94:240-55. 2006
    ..The proposed mechanism of electrophilic neurotoxicants represents a new and exciting experimental framework for mechanistic research in human neuropathological conditions associated with toxicant exposure or disease-based processes...
  8. ncbi request reprint Structure-toxicity analysis of type-2 alkenes: in vitro neurotoxicity
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467, USA
    Toxicol Sci 95:136-46. 2007
    ..This is consistent with our hypothesis that the mechanism of ACR neurotoxicity involves formation of Michael adducts with protein sulfhydryl groups...
  9. ncbi request reprint Neurotoxic mechanisms of electrophilic type-2 alkenes: soft soft interactions described by quantum mechanical parameters
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111 E 210th St, Bronx, New York 10467, USA
    Toxicol Sci 98:561-70. 2007
    ..These findings suggest that the neurotoxicity of ACR and its conjugated alkene analogs is related to electrophilic softness and that the thiolate state of cysteine residues is the corresponding adduct target...
  10. pmc Molecular mechanisms of the conjugated alpha,beta-unsaturated carbonyl derivatives: relevance to neurotoxicity and neurodegenerative diseases
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467 2490, USA
    Toxicol Sci 104:235-49. 2008
    ....
  11. doi request reprint Type-2 alkenes mediate synaptotoxicity in neurodegenerative diseases
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Moses Research Tower 7, 111 East 2210th Street, Bronx, NY 10467 2490, United States
    Neurotoxicology 29:871-82. 2008
    ..Based on a presumed common nerve terminal site of action, we propose that the onset and progression of this neuropathogenic process is accelerated by environmental exposure to other type-2 alkenes...
  12. doi request reprint Acrylamide-induced nerve terminal damage: relevance to neurotoxic and neurodegenerative mechanisms
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111 East 210th Street, Bronx, New York 10467, USA
    J Agric Food Chem 56:5994-6003. 2008
    ....
  13. doi request reprint Molecular mechanisms of 4-hydroxy-2-nonenal and acrolein toxicity: nucleophilic targets and adduct formation
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111 East 210th Street, Bronx, New York 10467, USA
    Chem Res Toxicol 22:1499-508. 2009
    ..Understanding the molecular actions of acrolein and HNE could provide insight into many pathogenic conditions that involve initial cellular oxidative stress and could, thereby, offer new efficacious avenues of pharmacological defense...
  14. ncbi request reprint 2,5-Hexanedione-induced changes in the neurofilament subunit pools of rat peripheral nerve
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Moses Research Tower 7, 111 E 210th St, Bronx, NY 10467, USA
    Neurotoxicology 26:229-40. 2005
    ..These results suggest that HD intoxication is primarily associated with depletion of soluble NF proteins, which could produce axon atrophy through disruption of cytoskeletal turnover and maintenance...
  15. ncbi request reprint gamma-Diketone neuropathy: axon atrophy and the role of cytoskeletal protein adduction
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467 2490, USA
    Toxicol Appl Pharmacol 199:20-34. 2004
    ..Evidence presented in this review suggests that "distal axonopathy" is an inaccurate classification and future nosological schemes should be based on the apparent primacy of axon atrophy...
  16. ncbi request reprint Rate of neurotoxicant exposure determines morphologic manifestations of distal axonopathy
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine Montefiore Medical Center, 111 E 210th Street, Bronx, New York, 10467 2490, USA
    Toxicol Appl Pharmacol 167:75-86. 2000
    ..In this commentary we will review the evidence for the dose-related dependency of distal axonopathies and discuss how this concept might influence our current understanding of chemical-induced neurotoxicities...
  17. ncbi request reprint Effects of ion channel blockade on the distribution of Na, K, Ca and other elements in oxygen-glucose deprived CA1 hippocampal neurons
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467, USA
    Neuroscience 103:971-83. 2001
    ..Thus, we suggest that voltage-gated tetrodotoxin-sensitive Na+ channels and glutamate-gated ionotropic NMDA or AMPA receptors are important routes of ion flux during nerve cell injury induced by oxygen-glucose deprivation/reperfusion...
  18. ncbi request reprint Nerve terminals as the primary site of acrylamide action: a hypothesis
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467, USA
    Neurotoxicology 23:43-59. 2002
    ..Our proposal suggests a reevaluation of current classification schemes and mechanistic hypotheses that regard ACR axonopathy as a primary lesion...
  19. ncbi request reprint Neurological evaluation of toxic axonopathies in rats: acrylamide and 2,5-hexanedione
    R M LoPachin
    Neurotoxicology 23:95-110. 2002
    ..These results suggest that observations of spontaneous behavior in an open field represent a practical approach to assessing temporal development and extent of neurological dysfunction induced by axonopathic toxicants such as ACR and HD...
  20. ncbi request reprint The role of fast axonal transport in acrylamide pathophysiology: mechanism or epiphenomenon?
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467, USA
    Neurotoxicology 23:253-7; discussion 265-70. 2002
  21. ncbi request reprint Acrylamide axonopathy revisited
    R M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111 E 210th St, Bronx, NY 10467, USA
    Toxicol Appl Pharmacol 188:135-53. 2003
    ..These findings have important implications for future mechanistic research, classification schemes, and assessment of neurotoxicity risk...
  22. ncbi request reprint gamma-diketone central neuropathy: quantitative morphometric analysis of axons in rat spinal cord white matter regions and nerve roots
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467 2490, USA
    Toxicol Appl Pharmacol 193:29-46. 2003
    ..Appl. Pharmacol. 135 (1995) 58; and Toxicol. Appl. Pharmacol. 165 (2000) 127) indicate that axon atrophy, and not axonal swelling, is a primary neuropathic phenomenon...
  23. ncbi request reprint Application of proteomics to the study of molecular mechanisms in neurotoxicology
    Richard M LoPachin
    Department of Anesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Moses 7, 111 E, 210th St, Bronx, NY 10467, USA
    Neurotoxicology 24:761-75. 2003
    ....
  24. ncbi request reprint In vivo and in vitro effects of acrylamide on synaptosomal neurotransmitter uptake and release
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Moses Research Tower 7, 111 E 210th Street, Bronx, NY 10467, USA
    Neurotoxicology 25:349-63. 2004
    ..These data suggest that ACR-induced synaptic dysfunction involves adduction of presynaptic protein thiol groups and subsequent reduction in neurotransmitter release...
  25. ncbi request reprint The changing view of acrylamide neurotoxicity
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Anesthesia Research, Moses 7, Montefiore Medical Center, 111 E 210th St, Bronx, NY 10467, USA
    Neurotoxicology 25:617-30. 2004
    ..Adduction of thiol groups also might be common to the reproductive and carcinogenic effects of ACR. A final goal of this review is to identify data gaps that retard a comprehensive understanding of ACR pathophysiological processes...
  26. ncbi request reprint 2,5-Hexanedione-induced changes in the monomeric neurofilament protein content of rat spinal cord fractions
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467, USA
    Toxicol Appl Pharmacol 198:61-73. 2004
    ..The selective depletion of Triton-soluble protein suggested that HD adduction of NFs interfered with the dynamic interactions of the polymeric and mobile monomeric pools...
  27. ncbi request reprint Acrylamide toxicities and food safety: Session IV summary and research needs
    Richard M LoPachin
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, 111E 210th Street, Bronx, NY 10467, USA
    Neurotoxicology 25:507-9. 2004
  28. pmc Gamma-diketone axonopathy: analyses of cytoskeletal motors and highways in CNS myelinated axons
    Lihai Zhang
    Department of Anesthesiology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York 10467 2490, USA
    Toxicol Sci 117:180-9. 2010
    ..Given their critical role in cytoskeletal physiology, MAPs could represent a relevant target for the induction of gamma-diketone axonopathy...

Research Grants25

  1. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2003
    ..In addition, our studies might suggest novel pharmacotherapies based on neurotrophin intervention or replacement. ..
  2. The Nerve Terminal as a Site of Acrylamide Action
    RICHARD LOPACHIN; Fiscal Year: 2006
    ..Determining how these chemicals work will provide a rational basis for establishing occupational exposure conditions and for development of efficacious pharmacotherapeutic approaches. ..
  3. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2007
    ..Understanding the role and mechanism of axon atrophy in solvent neurotoxicity has broad-based implications for human occupational health and risk assessment. ..
  4. The Nerve Terminal as the Site of Action for Type-2 Alkenes
    RICHARD LOPACHIN; Fiscal Year: 2009
    ....
  5. NERVE TERMINAL AS A SITE OF ACRYLAMIDE ACTION
    RICHARD LOPACHIN; Fiscal Year: 2002
    ..Results could lead to a better understanding of acquired and inherited human neuropathies and the development of efficacious pharmacotherapies. ..
  6. MOLECULAR MECHANISMS OF HEXACARBON INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 1999
    ..In addition, this proposal might have broad-based implications for other toxic chemicals (acrylamide, carbon disulfide) and disease processes (diabetes) associated with axonal atrophy. ..
  7. The Nerve Terminal as the Site of Action for Type-2 Alkenes
    RICHARD MICHAEL LOPACHIN; Fiscal Year: 2010
    ....
  8. MOLECULAR MECHANISMS OF HEXACARBON-INDUCED AXON ATROPHY
    RICHARD LOPACHIN; Fiscal Year: 2009
    ..Understanding the role and mechanism of axon atrophy in solvent neurotoxicity has broad-based implications for human occupational health and risk assessment. ..