Research Topics
Genomes and Genes | Simon KwokSummaryAffiliation: Albert Einstein Medical Center Location: Philadelphia, USA Summary: Basic science research in the areas of Alzheimers Disease, prostate cancer and reproduction. Publications
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Publications
Evaluation of elution and mechanical properties of two injectable chemotherapeutic bone cementsJohn A Handal
Department of Orthopedic Surgery, Albert Einstein Medical Center, Philadelphia, PA, USA
Chemotherapy 57:268-74. 2011..However, the elution and mechanical properties of each bone cement-drug amount combination should be thoroughly quantified in vitro before using such a combination in a clinical setting...
Placental lactogen-I variant utilizes the prolactin receptor signaling pathwayC B Cohick
Department of Physiology, University of Kansas Medical Center, Kansas City 66160 7401, USA
Mol Cell Endocrinol 116:49-58. 1996..Each of these actions is consistent with PL-Iv utilizing the PRL receptor signal transduction pathway...- Recombinant porcine prorelaxin produced in Chinese hamster ovary cells is biologically activeA L Vu
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160
Life Sci 52:1055-61. 1993..No conversion of the 19 kDa prorelaxin into the 6 kDa relaxin was observed. Unlike most prohormones which are biologically inactive, the recombinant prorelaxin was found to be biologically active in an in vitro bioassay... - Heterologous expression and characterization of prolactin-like protein-A. Identification of serum binding proteinsS Deb
Department of Physiology, University of Kansas Medical Center, Kansas City 66160
J Biol Chem 268:3298-305. 1993..Binding of PLP-A to serum proteins was specific for PLP-A and not effectively competed by other members of the prolactin/growth hormone family. The PLP-A binding species were also found in serum from non-pregnant female and male rats... - Molecular cloning and sequence analysis of two porcine seminal proteins, PSP-I and PSP-II: new members of the spermadhesin familyS C Kwok
Department of Obstetrics and Gynecology, Albert Einstein Medical Center, Philadelphia, PA 19141
DNA Cell Biol 12:605-10. 1993..This suggests that these two genes evolved by duplication and divergence from a common ancestral gene. They share about 50% amino acid sequence homology and a similar overall structure with three members of the spermadhesin family... - Binding characteristics and immunolocalization of porcine seminal protein, PSP-IS C Kwok
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City
Mol Reprod Dev 35:244-50. 1993.... - Transcriptional activation of cytochrome P450 side chain cleavage enzyme expression during trophoblast cell differentiationT Yamamoto
Department of Physiology, University of Kansas Medical Center, Kansas City 66160
J Biol Chem 269:6517-23. 1994..In conclusion, transcriptional activation of the P450scc gene accompanies trophoblast giant cell differentiation... - Molecular cloning and sequence analysis of the cDNA encoding porcine acrosin inhibitorS C Kwok
Department of Obstetrics and Gynecology, Albert Einstein Medical Center, Philadelphia, PA 19141
DNA Cell Biol 13:389-94. 1994..Southern blot analysis shows only a single hybridizing band with three different restriction endonucleases, suggesting the presence of a single copy of the acrosin inhibitor gene in the porcine genome... - Decidual prolactin-related protein. Identification, molecular cloning, and characterizationK F Roby
Department of Physiology, University of Kansas Medical Center, Kansas City 66160
J Biol Chem 268:3136-42. 1993..0-kilobase mRNA. The relationship of dPRP with other members of the PRL-GH family and its putative role(s) in the physiology of pregnancy are discussed... - Characterization of multiple prohormone convertase PC1/3 transcripts in porcine ovaryG Dai
Department of Obstetrics and Gynecology, Albert Einstein Medical Center, Philadelphia, PA 19141 3098, USA
Biochim Biophys Acta 1264:1-6. 1995..Furthermore, five different polyadenylation sites have been observed. The utilization of these polyadenylation sites results in a length difference of 40-440 bp in the 3' untranslated regions of the transcripts... - Relaxin purification from human placental basal platesS Yamamoto
J Clin Endocrinol Metab 52:601-4. 1981.... - Prolactin-like protein-C variant: complementary deoxyribonucleic acid, unique six exon gene structure, and trophoblast cell-specific expressionG Dai
Department of Physiology, University of Kansas Medical Center, Kansas City 66160, USA
Endocrinology 137:5009-19. 1996..In conclusion, we have identified a new member of the PRL family possessing considerable homology to PLP-C, a unique gene structure, and displaying a trophoblast-specific pattern of transcriptional activation... - Rcho-1 trophoblast cell placental lactogens: complementary deoxyribonucleic acids, heterologous expression, and biological activitiesG Dai
Department of Physiology, University of Kansas Medical Center, Kansas City 66160, USA
Endocrinology 137:5020-7. 1996..Additionally, both recombinant Rcho-1 PL-I and PL-II proteins are biologically active... - Atorvastatin activates heme oxygenase-1 at the stress response elementsSimon C M Kwok
ORTD, Albert Einstein Medical Center, Philadelphia, PA 19141 3098, USA
J Cell Mol Med 16:394-400. 2012..Because these StRE sites are present in clusters in HO-1 promoter, up-regulation of HO-1 by atorvastatin may involve multiple StRE sites. The role of HO-1 in atorvastatin-induced apoptosis in PC-3 and MCF-7 remains to be studied... - Brefeldin A activates CHOP promoter at the AARE, ERSE and AP-1 elementsSimon C M Kwok
Department of Pathology and Laboratory Medicine, Albert Einstein Medical Center, Philadelphia, PA 19141 3098, USA
Mol Cell Biochem 319:203-8. 2008..Our results suggest that although CHOP is up-regulated by brefeldin A, it is not a major mediator of brefeldin A-induced apoptosis... - PTX1(ERGIC2)-VP22 fusion protein upregulates interferon-beta in prostate cancer cell line PC-3Simon C M Kwok
Department of Pathology and Laboratory Medicine, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141 3098, USA
DNA Cell Biol 25:523-9. 2006..Upregulation of IFN-beta was confirmed by RTPCR and promoter-reporter assay. However, the upregulation of IFN-beta by the PTX1-VP22 fusion protein was not due to nuclear translocation of the PTX1 luminal domain... - Effects of PTX1 expression on growth and tumorigenicity of the prostate cancer cell line PC-3Xianhong Liu
Department of Pathology and Laboratory Medicine, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141 3098, USA
DNA Cell Biol 22:469-74. 2003..In nude mice, cells expressing antisense PTX1 grew sixfold faster than the control. These results suggest that PTX1 may play an important role in the growth and tumorigenicity of PC-3 cells... - Molecular cloning and sequence analysis of a human untranslated Alu-containing RNAS C Kwok
Department of Pathology and Laboratory Medicine, Albert Einstein Medical Center, 5501 Old York Road, Korman 214, Philadelphia, PA 19141 3098, USA
DNA Seq 12:85-9. 2001..Hence, it belongs to a class of untranslated Alu-containing RNAs. LNX1 is expressed in most tissues. It is encoded by a single copy gene, which is localized on human chromosome 14... - Molecular cloning, expression, localization, and gene organization of PTX1, a human nuclear protein that is downregulated in prostate cancerS C Kwok
Department of Pathology and Laboratory Medicine, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141 3098, USA
DNA Cell Biol 20:349-57. 2001..The nuclear staining was greatly reduced in prostate carcinoma. The gene organization of PTX1 was established by comparing the cDNA sequence with the published human genomic sequence... - Expression and localization of prohormone convertase 1/3 (SPC3) in porcine ovaryR H Renegar
Department of Anatomy and Cell Biology, Brody School of Medicine at East Carolina University, Greenville, North Carolina, USA
Mol Reprod Dev 57:361-5. 2000..Levels of PC1/3 transcripts in corpora lutea increased as gestation advanced, parallel with an observed increase in relaxin transcripts. A role for PC1/3 in proprotein processing in the ovary is discussed... - Immunostimulatory effects of pig seminal proteins on pig lymphocytesL S Leshin
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA
J Reprod Fertil 114:77-84. 1998.... - Purified porcine seminal plasma protein enhances in vitro immune activities of porcine peripheral lymphocytesW C Yang
Department of Health and Human Services, Food and Drug Administration, Rockville, Maryland 20857, USA
Biol Reprod 59:202-7. 1998..The high potency of PSP on lymphocyte activities and the abundance of PSP in seminal plasma have suggested that PSP may play an important role in regulating immune responses in the porcine uterine environment... 
Extensive restriction site polymorphism at the human phenylalanine hydroxylase locus and application in prenatal diagnosis of phenylketonuriaA S Lidsky
Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA
Am J Hum Genet 37:619-34. 1985..5%. Thus, most families with a history of classical phenylketonuria can take advantage of the genetic analysis for prenatal diagnosis and carrier detection of the hereditary disorder...- Isolation and characterization of high molecular weight forms of relaxinS C Kwok
Adv Exp Med Biol 143:209-13. 1982 - The biosynthesis of insulin: some genetic and evolutionary aspectsS J Chan
Diabetes Care 4:4-10. 1981 - Evidence for proteolysis during purification of relaxin from pregnant sow ovariesS C Kwok
Endocr Res Commun 7:1-12. 1980..However the ratio of the three forms of relaxin remain unchanged... - Transplantable rat choriocarcinoma cells express placental lactogen: identification of placental lactogen-I immunoreactive protein and messenger ribonucleic acidT N Faria
Department of Physiology, University of Kansas Medical Center, Kansas City 66103
Endocrinology 127:3131-7. 1990..The Rcho tumor resembles rat trophoblast tissue at early postimplantation stages (days 6-10 of gestation) and may be a useful tool for studying placental PRL expression during trophoblast differentiation... - Ontogeny of placental lactogen-I and placental lactogen-II expression in the developing rat placentaT N Faria
Department of Physiology, University of Kansas Medical Center, Kansas City 66103
Dev Biol 141:279-91. 1990.... - Identification and characterization of a new member of the prolactin family, placental lactogen-I variantS Deb
Department of Physiology, University of Kansas Medical Center, Kansas City 66103
J Biol Chem 266:1605-10. 1991..In summary, PL-Iv shares structural similarities with PL-I; however, it shows other structural differences in addition to unique cell- and temporal-specific patterns of expression in the rat chorioallantoic placenta... - Expression of alkaline phosphatase in differentiated rat labyrinthine trophoblast tissueW J Campbell
Department of Physiology, Ralph L Smith Mental Retardation Research Center, University of Kansas Medical Center, Kansas City 66103
Placenta 12:227-37. 1991..Enzymatic deglycosylation of the 75- and 95-kDa alkaline phosphatase species resulted in the generation of a 55-kDa species. In summary, alkaline phosphatase expression is a useful indicator of trophoblast differentiation... - Molecular cloning and characterization of prolactin-like protein C complementary deoxyribonucleic acidS Deb
Department of Physiology, University of Kansas Medical Center, Kansas City 66103
J Biol Chem 266:23027-32. 1991..In situ hybridization analysis indicated that PLP-C mRNA was specifically expressed by spongiotrophoblast cells and some trophoblast giant cells in the junctional zone region of rat chorioallantoic placenta... - Purification and characterization of PSP-I and PSP-II, two major proteins from porcine seminal plasmaK J Rutherfurd
Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010 0269
Arch Biochem Biophys 295:352-9. 1992..PSP-II shares 50% sequence homology with a family of zona pellucida-binding glycoproteins at the N-terminus... - Purification and characterization of recombinant porcine prorelaxin expressed in Escherichia coliG K Reddy
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160
Arch Biochem Biophys 294:579-85. 1992..Both the recombinant prorelaxin and converted relaxin were found to be biologically active in an in vitro bioassay for relaxin... - High molecular weight forms of relaxin in pregnant sow ovariesS C Kwok
Biochem Biophys Res Commun 82:997-1005. 1978 - GABA receptor agonists: relationship between structure and biological activity in vivo and in vitroP Krogsgaard-Larsen
Adv Exp Med Biol 123:303-21. 1979 - What happens with palatal dysfunction?P Mayoral
J Clin Orthod 12:561-5. 1978 - Cloning and nucleotide sequence analysis of the human beta-microseminoprotein geneC B Green
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66103
Biochem Biophys Res Commun 167:1184-90. 1990..Restriction endonuclease mapping and sequence analysis of this clone revealed the human MSP gene of approximately 15 Kb in length. The gene contains four exons and three large introns of approximately 6, 1, and 7 Kb... - Homology between phenylalanine and tyrosine hydroxylases reveals common structural and functional domainsF D Ledley
Biochemistry 24:3389-94. 1985..The degree of homology also suggests that these two proteins evolved from a common ancestor... - Familial hyperinsulinemia due to a structurally abnormal insulin. Definition of an emerging new clinical syndromeM Haneda
N Engl J Med 310:1288-94. 1984..Glucose tolerance may range from relatively normal to overtly diabetic... - Identification of a point mutation in the human insulin gene giving rise to a structurally abnormal insulin (insulin Chicago)S C Kwok
Diabetes 32:872-5. 1983..This point mutation leads to the substitution of a leucine for phenylalanine accompanied by the loss of a restriction endonuclease Mboll recognition site and the creation of a new Rsal cleavage site at this position... - Studies on mutant human insulin genes: identification and sequence analysis of a gene encoding [SerB24]insulinM Haneda
Proc Natl Acad Sci U S A 80:6366-70. 1983..Both insulin alleles were of the alpha type and, aside from a single nucleotide deletion in the 5' region of the normal allele, their sequences were identical to those previously determined... - Cloning and nucleotide sequence analysis of the dog insulin gene. Coded amino acid sequence of canine preproinsulin predicts an additional C-peptide fragmentS C Kwok
J Biol Chem 258:2357-63. 1983..The revised C-peptide sequence suggests that the proinsulin C-peptide is more highly conserved in length and overall sequence than was previously supposed... - Messenger RNA sequence and primary structure of preproinsulin in a primitive vertebrate, the Atlantic hagfishS J Chan
J Biol Chem 256:7595-602. 1981 - Loss of a restriction endonuclease cleavage site in the gene of a structurally abnormal human insulinS C Kwok
Biochem Biophys Res Commun 98:844-9. 1981 - Molecular structure and polymorphic map of the human phenylalanine hydroxylase geneA G DiLella
Biochemistry 25:743-9. 1986.... - The human phenylalanine hydroxylase geneS L Woo
Prog Clin Biol Res 177:123-35. 1985 - Nucleotide sequence of a full-length complementary DNA clone and amino acid sequence of human phenylalanine hydroxylaseS C Kwok
Biochemistry 24:556-61. 1985..The human protein shows a 96% amino acid sequence homology with the corresponding rat enzyme. The determination of the complete primary structure for phenylalanine hydroxylase represents the first among mixed-function oxidases... - Differential expression of placental lactogen-II and prolactin-like protein-A in the rat chorioallantoic placentaW J Campbell
Department of Physiology, Ralph L Smith, Mental Retardation Research Center, University of Kansas Medical Center, Kansas City 66103
Endocrinology 125:1565-74. 1989..PLP-A is also expressed by spongiotrophoblast cells of the junctional zone. These findings provide insights into the process of placental morphogenesis... - Target tissues for relaxin identified in vitro with 125I-labelled porcine relaxinJ P McMurtry
J Reprod Fertil 53:209-16. 1978..Under the conditions used, no relaxin receptors were noted in the liver, spleen or heart from any of the species investigated...