MARGARET C KIELIAN

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. pmc Domain III from class II fusion proteins functions as a dominant-negative inhibitor of virus membrane fusion
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 171:111-20. 2005
  2. ncbi request reprint Class II virus membrane fusion proteins
    Margaret Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Virology 344:38-47. 2006
  3. ncbi request reprint Virus membrane-fusion proteins: more than one way to make a hairpin
    Margaret Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Rev Microbiol 4:67-76. 2006
  4. pmc Mechanisms of mutations inhibiting fusion and infection by Semliki Forest virus
    M Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Cell Biol 134:863-72. 1996
  5. pmc Dealing with low pH: entry and exit of alphaviruses and flaviviruses
    Claudia Sánchez-San Martín
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Trends Microbiol 17:514-21. 2009
  6. pmc In vitro reconstitution reveals key intermediate states of trimer formation by the dengue virus membrane fusion protein
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 84:5730-40. 2010
  7. pmc Multistep regulation of membrane insertion of the fusion peptide of Semliki Forest virus
    Don L Gibbons
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 78:3312-8. 2004
  8. pmc E1 mutants identify a critical region in the trimer interface of the Semliki forest virus fusion protein
    Catherine Y Liu
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 83:11298-306. 2009
  9. pmc Differential cholesterol binding by class II fusion proteins determines membrane fusion properties
    M Umashankar
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 82:9245-53. 2008
  10. pmc Second-site revertants of a Semliki Forest virus fusion-block mutation reveal the dynamics of a class II membrane fusion protein
    Chantal Chanel-Vos
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Virol 80:6115-22. 2006

Collaborators

  • Felix A Rey
  • S Ghosh
  • Maofu Liao
  • Don L Gibbons
  • Claudia Sánchez-San Martín
  • Catherine Y Liu
  • Xinyong Zhang
  • Chantal Chanel-Vos
  • Anna Ahn
  • Brigid Reilly
  • Zhao Ling Qin
  • M Umashankar
  • Gwen M Taylor
  • Prodyot K Chatterjee
  • Yifan Song
  • Christen Besanceney
  • Aihua Zheng
  • Yan Zheng
  • Gwen Taylor
  • Alice Guo
  • Hernando Sosa
  • Phyllis I Hanson
  • Lena Hammar
  • Armelle Vigouroux
  • R Holland Cheng
  • Marie Christine Vaney
  • Robert Day
  • Jorge Navaza
  • Jean Lepault
  • Martin Fugere
  • Inge Erk
  • Christina H Eng

Detail Information

Publications27

  1. pmc Domain III from class II fusion proteins functions as a dominant-negative inhibitor of virus membrane fusion
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 171:111-20. 2005
    ..These novel inhibitors of the class II fusion proteins show cross-inhibition within the virus genus and suggest that the domain III-core trimer interaction can serve as a new target for the development of antiviral reagents...
  2. ncbi request reprint Class II virus membrane fusion proteins
    Margaret Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Virology 344:38-47. 2006
    ..Inhibition of the fusion protein refolding reaction confirms its importance in fusion and suggests new antiviral strategies for these medically important viruses...
  3. ncbi request reprint Virus membrane-fusion proteins: more than one way to make a hairpin
    Margaret Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Rev Microbiol 4:67-76. 2006
    ..This review will focus in particular on the properties of the more recently described class II proteins...
  4. pmc Mechanisms of mutations inhibiting fusion and infection by Semliki Forest virus
    M Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Cell Biol 134:863-72. 1996
    ....
  5. pmc Dealing with low pH: entry and exit of alphaviruses and flaviviruses
    Claudia Sánchez-San Martín
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Trends Microbiol 17:514-21. 2009
    ..We discuss results using truncated proteins to dissect the fusion reaction, and future research directions including the development of antiviral therapies against these medically important viruses...
  6. pmc In vitro reconstitution reveals key intermediate states of trimer formation by the dengue virus membrane fusion protein
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 84:5730-40. 2010
    ..Truncated DV E proteins thus reconstitute hairpin formation and define properties of key domain interactions during DV fusion...
  7. pmc Multistep regulation of membrane insertion of the fusion peptide of Semliki Forest virus
    Don L Gibbons
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 78:3312-8. 2004
    ..Thus, the membrane insertion of the E1 fusion peptide is regulated by additional low-pH-dependent steps after exposure, perhaps involving an E1-cholesterol interaction...
  8. pmc E1 mutants identify a critical region in the trimer interface of the Semliki forest virus fusion protein
    Catherine Y Liu
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 83:11298-306. 2009
    ..While there are extensive contacts between E1 subunits in the homotrimer, the D188K mutant identifies an important "hot spot" for protein-protein interactions within the core trimer...
  9. pmc Differential cholesterol binding by class II fusion proteins determines membrane fusion properties
    M Umashankar
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 82:9245-53. 2008
    ..SFV E1 is the first virus fusion protein demonstrated to directly bind cholesterol. Taken together, our results reveal important functional differences conferred by the cholesterol-binding properties of class II fusion proteins...
  10. pmc Second-site revertants of a Semliki Forest virus fusion-block mutation reveal the dynamics of a class II membrane fusion protein
    Chantal Chanel-Vos
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Virol 80:6115-22. 2006
    ..Together the revertants reveal specific and interconnected aspects of the fusion protein refolding reaction...
  11. pmc Site-directed antibodies against the stem region reveal low pH-induced conformational changes of the Semliki Forest virus fusion protein
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 80:9599-607. 2006
    ..Our data suggest that E1 hairpin formation occurs by the sequential packing of domain III and the stem onto the trimer core and indicate a tight correlation between stem packing and membrane merger...
  12. pmc A conserved histidine in the ij loop of the Semliki Forest virus E1 protein plays an important role in membrane fusion
    Chantal Chanel-Vos
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 78:13543-52. 2004
    ..Our results indicate that the E1 ij loop and the conserved H230 residue play a critical role in alphavirus-membrane fusion and suggest the presence of a previously undescribed late intermediate in the fusion reaction...
  13. pmc A stable prefusion intermediate of the alphavirus fusion protein reveals critical features of class II membrane fusion
    Claudia Sánchez-San Martín
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell Host Microbe 4:600-8. 2008
    ..Even in the absence of DIII, DI/II trimers interacted to form hexagonal lattices and to cause membrane deformation and tubulation. These studies identify a prefusion intermediate in class II membrane fusion...
  14. pmc Role of conserved histidine residues in the low-pH dependence of the Semliki Forest virus fusion protein
    Zhao Ling Qin
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 83:4670-7. 2009
    ..Together, these results and the location of H3 suggest that this residue acts to regulate the low-pH-dependent refolding of E1 during membrane fusion...
  15. pmc Novel mutations that control the sphingolipid and cholesterol dependence of the Semliki Forest virus fusion protein
    Prodyot K Chatterjee
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Virol 76:12712-22. 2002
    ..Thus, the srf-4 and srf-5 mutations identify novel regions of E1 that are distinct from the fusion peptide and srf-3 region and modulate the requirements for both sphingolipid and cholesterol in virus-membrane fusion...
  16. pmc Pseudorevertants of a Semliki forest virus fusion-blocking mutation reveal a critical interchain interaction in the core trimer
    Catherine Y Liu
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Virol 84:11624-33. 2010
    ..Together, our data support a model in which a ring of three salt bridges formed by D188 and K176 stabilize the core trimer, a key intermediate of the alphavirus fusion protein...
  17. pmc The fusion peptide of Semliki Forest virus associates with sterol-rich membrane domains
    Anna Ahn
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 76:3267-75. 2002
    ....
  18. pmc Furin processing and proteolytic activation of Semliki Forest virus
    Xinyong Zhang
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 77:2981-9. 2003
    ..However, the in vivo infectivity of mutant L was more strongly inhibited than that of wt/p62, due to additional effects of the mutation on virus-cell binding...
  19. ncbi request reprint Visualization of the target-membrane-inserted fusion protein of Semliki Forest virus by combined electron microscopy and crystallography
    Don L Gibbons
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell 114:573-83. 2003
    ..Our data allow the visualization of a viral fusion protein inserted in its target membrane and demonstrate that insertion is a cooperative process, resulting in rings composed of five to six homotrimers...
  20. pmc Purification and crystallization reveal two types of interactions of the fusion protein homotrimer of Semliki Forest virus
    Don L Gibbons
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 78:3514-23. 2004
    ..Determination of the structure will provide our first high-resolution views of both the low-pH-induced trimeric conformation and the target membrane-interacting region of the alphavirus fusion protein...
  21. ncbi request reprint The conserved glycine residues in the transmembrane domain of the Semliki Forest virus fusion protein are not required for assembly and fusion
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Virology 332:430-7. 2005
    ..TM domain glycine residues thus are not required for efficient SFV fusion or assembly but can cause subtle effects on the properties of membrane fusion...
  22. ncbi request reprint An interaction site of the envelope proteins of Semliki Forest virus that is preserved after proteolytic activation
    Xinyong Zhang
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Virology 337:344-52. 2005
    ..Our data suggest that there is an important p62/E1 dimer interaction site identified by an E2 R250G mutation and that this interaction is maintained after processing to the mature E2 protein...
  23. ncbi request reprint Structural surprises from the flaviviruses and alphaviruses
    Margaret Kielian
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cell 9:454-6. 2002
    ..These differences raise many interesting questions about virus assembly and fusion activity...
  24. ncbi request reprint Mutations that promote furin-independent growth of Semliki Forest virus affect p62-E1 interactions and membrane fusion
    Xinyong Zhang
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Virology 327:287-96. 2004
    ..Sequence analysis of the pci mutants identified mutations primarily on the E2 protein, and suggested sites important in the interaction of p62 with E1 and the regulation of fusion...
  25. pmc Ubiquitin depletion and dominant-negative VPS4 inhibit rhabdovirus budding without affecting alphavirus budding
    Gwen M Taylor
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 81:13631-9. 2007
    ..In contrast, SFV budding was independent of both ubiquitin and the activity of VPS4, perhaps reflecting the important role of the highly organized envelope protein lattice during alphavirus budding...
  26. pmc Functions of the stem region of the Semliki Forest virus fusion protein during virus fusion and assembly
    Maofu Liao
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 80:11362-9. 2006
    ..The lack of a specific stem sequence requirement during SFV fusion suggests that the interaction of domain III with the trimer core can provide sufficient driving force to mediate membrane merger...
  27. pmc Molecular dissection of the Semliki Forest virus homotrimer reveals two functionally distinct regions of the fusion protein
    Don L Gibbons
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Virol 76:1194-205. 2002
    ..Together, our data identify two separate regions of the SFV E1 ectodomain, one responsible for target membrane association and one necessary for trimer interactions...

Research Grants5

  1. Training Program in Cellular and Molecular Biology and Genetics
    Margaret Kielian; Fiscal Year: 2007
    ..Ph. D. graduates develop the expertise, knowledge and critical abilities essential for a successful career in science. ..
  2. Inhibition of the Membrane Fusion Proteins of Flaviviruses and Alphaviruses
    Margaret Kielian; Fiscal Year: 2007
    ..This application focuses on developing new antiviral strategies for the flaviviruses and alphaviruses, based on blocking the activity of the proteins involved in the initial entry of the virus into the cell. ..