Lloyd Fricker

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. pmc Neuropeptide-processing enzymes: applications for drug discovery
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    AAPS J 7:E449-55. 2005
  2. ncbi request reprint Tissue distribution and characterization of soluble and membrane-bound forms of metallocarboxypeptidase D
    L Song
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 271:28884-9. 1996
  3. ncbi request reprint Carboxypeptidase E activity is deficient in mice with the fat mutation. Effect on peptide processing
    L D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 271:30619-24. 1996
  4. ncbi request reprint Peptides, enzymes and obesity: new insights from a 'dead' enzyme
    L D Fricker
    Dept of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
    Trends Biochem Sci 24:390-3. 1999
  5. ncbi request reprint Identification and characterization of proSAAS, a granin-like neuroendocrine peptide precursor that inhibits prohormone processing
    L D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 20:639-48. 2000
  6. ncbi request reprint Protein phosphatase 2A binds to the cytoplasmic tail of carboxypeptidase D and regulates post-trans-Golgi network trafficking
    O Varlamov
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Sci 114:311-22. 2001
  7. ncbi request reprint Neuropeptidomics to study peptide processing in animal models of obesity
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Endocrinology 148:4185-90. 2007
  8. ncbi request reprint Peptidomics: identification and quantification of endogenous peptides in neuroendocrine tissues
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mass Spectrom Rev 25:327-44. 2006
  9. pmc Analysis of mouse brain peptides using mass spectrometry-based peptidomics: implications for novel functions ranging from non-classical neuropeptides to microproteins
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Biosyst 6:1355-65. 2010
  10. pmc Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding
    Peter J Lyons
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, United States of America
    PLoS ONE 5:e12967. 2010

Research Grants

  1. Neuropeptides, Processing Enzymes, and Drug Abuse
    Lloyd Fricker; Fiscal Year: 2009
  2. STRUCTURE/FUNCTION OF CARBOXYPEPTIDASE D
    Lloyd Fricker; Fiscal Year: 2003
  3. CELL BIOLOGY OF CARBOXYPEPTIDASE D
    Lloyd Fricker; Fiscal Year: 2003
  4. Quantitative Peptidomics of Food Deprivation
    Lloyd Fricker; Fiscal Year: 2005
  5. Neuropeptides, Processing Enzymes, and Drug Abuse
    Lloyd Fricker; Fiscal Year: 2007
  6. Function of Carboxypeptidase D
    Lloyd Fricker; Fiscal Year: 2007
  7. ProSAAS,A Novel Peptide Substrate of Carboxypeptidase E
    Lloyd Fricker; Fiscal Year: 2005
  8. Peptidomics of Cocaine and Amphetamine Abuse
    Lloyd Fricker; Fiscal Year: 2005
  9. REGULATION OF NEUROPEPTIDE PROCESSING ENZYMES
    Lloyd Fricker; Fiscal Year: 2002
  10. CELL BIOLOGY OF CARBOXYPEPTIDASE E/ENKEPHALIN CONVERTASE
    Lloyd Fricker; Fiscal Year: 1999

Collaborators

  • L A Devi
  • Jihyeon Lim
  • J Sun
  • Peter Arvan
  • L Song
  • S D Patterson
  • Jonathan Sweedler
  • Sean M J McBride
  • Sandra E Reznik
  • E H Leiter
  • N E Baker
  • William Wetsel
  • Min Li
  • Nino Mzhavia
  • Fa Yun Che
  • Elena Kalinina
  • Xin Zhang
  • Hui Pan
  • Galyna Sidyelyeva
  • Yun Feng
  • Iryna Berezniuk
  • Julia S Gelman
  • Suwen Wei
  • Peter J Lyons
  • Donald F Steiner
  • Francesc X Aviles
  • Leandro M Castro
  • Jose D Fontenele-Neto
  • Quan Yuan
  • Reeta Biswas
  • Emer S Ferro
  • Juan Sironi
  • Jonathan H Wardman
  • Myrasol B Callaway
  • John E Pintar
  • Bonnie Peng
  • Xiaorong Zhu
  • Monica Rodriguez de la Vega
  • Joan L Arolas
  • Antoni Hermoso
  • Robert Day
  • Fabien M Décaillot
  • Josep Vendrell
  • Sudha Srinivasan
  • Regina Kuliawat
  • Elena V Kalinina
  • O Varlamov
  • Brian P Schoenfeld
  • Sandra Gagnon
  • Christian Wegener
  • Leung Hang Ma
  • Aaron J Bell
  • Izaura Y Hirata
  • Robert Baker
  • Kemal Sonmez
  • Lawrence Toll
  • Jose M Bautista
  • Myrasol Callaway
  • Amalia Diez
  • Rafael G Sevilla
  • Julia Lorenzo
  • Sebastian Tanco
  • Ilona Vathy
  • Stephen R Salton
  • Daniela Nanno
  • Donna O Bunch
  • William G Richards
  • Edward M Eddy
  • Guo X Luo
  • Audra A McKinzie
  • Nancy Levin
  • Timothy E McGraw
  • Akira Arimura
  • Ramona M Rodriguiz
  • Roneka L Ravenell
  • Richard Scheller
  • Se Ryoung Kim
  • Jason Bock
  • Jiayu Zhong
  • Edith Lanoue
  • Sonia Segura
  • Oleg Varlamov
  • E Kalinina
  • F Y Che

Detail Information

Publications49

  1. pmc Neuropeptide-processing enzymes: applications for drug discovery
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    AAPS J 7:E449-55. 2005
    ..However, studies with mutant animals that lack functional peptide-processing enzymes have facilitated the discovery of novel neuropeptides, many of which may be appropriate targets for therapeutics...
  2. ncbi request reprint Tissue distribution and characterization of soluble and membrane-bound forms of metallocarboxypeptidase D
    L Song
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 271:28884-9. 1996
    ..The finding that CPD is widely distributed suggests a broad role for this enzyme in the processing of proteins that transit the secretory pathway...
  3. ncbi request reprint Carboxypeptidase E activity is deficient in mice with the fat mutation. Effect on peptide processing
    L D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 271:30619-24. 1996
    ..Furthermore, the increase in levels of high molecular weight enkephalin peptides in the Cpefat/Cpefat mouse suggests that CPE is required for efficient peptide processing by the endopeptidases...
  4. ncbi request reprint Peptides, enzymes and obesity: new insights from a 'dead' enzyme
    L D Fricker
    Dept of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, NY 10461, USA
    Trends Biochem Sci 24:390-3. 1999
    ..Obesity due to the Cpe(fat) mutation is not caused by increased food consumption but, rather, is a result of defective nutrient partitioning, the exact mechanism of which remains to be elucidated...
  5. ncbi request reprint Identification and characterization of proSAAS, a granin-like neuroendocrine peptide precursor that inhibits prohormone processing
    L D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 20:639-48. 2000
    ..Purified proSAAS inhibits prohormone convertase 1 activity with an IC(50) of 590 nM but does not inhibit prohormone convertase 2. Taken together, proSAAS may represent an endogenous inhibitor of prohormone convertase 1...
  6. ncbi request reprint Protein phosphatase 2A binds to the cytoplasmic tail of carboxypeptidase D and regulates post-trans-Golgi network trafficking
    O Varlamov
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Sci 114:311-22. 2001
    ..These results are consistent with the model that PP2A is involved in the trafficking of proteins between a TGN recycling loop and a cell-surface recycling loop, but is not involved in the individual recycling loops...
  7. ncbi request reprint Neuropeptidomics to study peptide processing in animal models of obesity
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Endocrinology 148:4185-90. 2007
    ..The quantitative peptidomics approach has provided some insights toward the answer to this question...
  8. ncbi request reprint Peptidomics: identification and quantification of endogenous peptides in neuroendocrine tissues
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mass Spectrom Rev 25:327-44. 2006
    ..In this review, peptidomics techniques are discussed along with the major findings of many recent studies and future directions for the field...
  9. pmc Analysis of mouse brain peptides using mass spectrometry-based peptidomics: implications for novel functions ranging from non-classical neuropeptides to microproteins
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Biosyst 6:1355-65. 2010
    ....
  10. pmc Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding
    Peter J Lyons
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, United States of America
    PLoS ONE 5:e12967. 2010
    ..If mutations in CPA6 contribute to Duane syndrome, our results also suggest that Duane syndrome can be a chondrogenic rather than a myogenic or neurogenic developmental disorder...
  11. pmc Fishing for the hidden peptidome in health and disease (drug abuse)
    Lloyd D Fricker
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    AAPS J 12:679-82. 2010
    ..The overarching goals of this special issue involve defining the peptidome, identifying the current state of the field, and discussing methods to characterize the peptides, their receptors, and future needs for such measurements...
  12. pmc Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells
    Regina Kuliawat
    Division of Endocrinology and Department of Developmental Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Biol Cell 15:1690-701. 2004
    ....
  13. ncbi request reprint Peptidomics of Cpe fat/fat mouse hypothalamus: effect of food deprivation and exercise on peptide levels
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:4451-61. 2005
    ..Taken together, the differential regulation of these peptides in the two paradigms suggests that the regulation is not due to the lower body weight but to the manner in which the paradigms achieved this lower body weight...
  14. ncbi request reprint Quantitative neuropeptidomics of microwave-irradiated mouse brain and pituitary
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell Proteomics 4:1391-405. 2005
    ....
  15. ncbi request reprint The role of prohormone convertase-2 in hypothalamic neuropeptide processing: a quantitative neuropeptidomic study
    Hui Pan
    Department of Pharmacology, Albert Einstein College of Medicine, Bronx, New York, USA
    J Neurochem 98:1763-77. 2006
    ....
  16. ncbi request reprint Peptidomics of Cpefat/fat mouse hypothalamus and striatum: effect of chronic morphine administration
    Fabien M Décaillot
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY, USA
    J Mol Neurosci 28:277-84. 2006
    ..Taken together, these results provide interesting insights into endogenous neuropeptide systems that are modulated by morphine and suggest further experiments to link candidate peptides with long-term effects of morphine...
  17. ncbi request reprint Optimization of neuropeptide extraction from the mouse hypothalamus
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Proteome Res 6:4667-76. 2007
    ..The hot water/cold acid extraction procedure resulted in the reproducible recovery of many hypothalamic peptides, including several novel peptides...
  18. pmc Peptidomics of Cpe(fat/fat) mouse brain regions: implications for neuropeptide processing
    Xin Zhang
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Neurochem 107:1596-613. 2008
    ..Taken together, these results indicate that CPE contributes, either directly or indirectly, to the production of the majority of neuropeptides...
  19. pmc Neuropeptidomic analysis establishes a major role for prohormone convertase-2 in neuropeptide biosynthesis
    Xin Zhang
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Neurochem 112:1168-79. 2010
    ....
  20. pmc Hemopressin and other bioactive peptides from cytosolic proteins: are these non-classical neuropeptides?
    Julia S Gelman
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    AAPS J 12:279-89. 2010
    ....
  21. ncbi request reprint Effect of voluntary exercise on genetically obese Cpefat/fat mice: quantitative proteomics of serum
    Quan Yuan
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Obes Res 12:1179-88. 2004
    ..To compare the effect of voluntary exercise on body weight, food consumption, and levels of serum proteins between wild-type and carboxypeptidase E-deficient (Cpefat/fat) mice...
  22. ncbi request reprint Relative quantitation of peptides in wild-type and Cpe(fat/fat) mouse pituitary using stable isotopic tags and mass spectrometry
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Mass Spectrom 40:227-37. 2005
    ..The finding that many peptides are substantially decreased in Cpe(fat/fat) pituitary is consistent with the broad role for carboxypeptidase E in the biosynthesis of numerous neuropeptides...
  23. ncbi request reprint Quantitative peptidomics of mouse pituitary: comparison of different stable isotopic tags
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Mass Spectrom 40:238-49. 2005
    ..In conclusion, both succinyl and TMAB isotopic labels are useful for quantitative peptidomics, and together these two labels provide more complete coverage of the endogenous peptides...
  24. pmc Hemopressins and other hemoglobin-derived peptides in mouse brain: comparison between brain, blood, and heart peptidome and regulation in Cpefat/fat mice
    Julia S Gelman
    Dominic P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurochem 113:871-80. 2010
    ..Taken together, these findings suggest that the post-translational processing of alpha and beta hemoglobin into the hemopressins, as well as other peptides, is up-regulated in some but not all Cpe(fat/fat) mouse brain regions...
  25. pmc CCP1/Nna1 functions in protein turnover in mouse brain: Implications for cell death in Purkinje cell degeneration mice
    Iryna Berezniuk
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    FASEB J 24:1813-23. 2010
    ....
  26. ncbi request reprint Neuropeptide-processing carboxypeptidases
    Suwen Wei
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Life Sci 73:655-62. 2003
    ..In the AtT-20 corticotroph cell line, CPA-5 has a perinuclear distribution. Taken together, these results are consistent with a role for CPA-5 in the intracellular processing of proopiomelanocortin-derived peptides at non-basic sites...
  27. ncbi request reprint Quantitation of neuropeptides in Cpe(fat)/Cpe(fat) mice using differential isotopic tags and mass spectrometry
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Anal Chem 74:3190-8. 2002
    ..The general method described in this report should be widely applicable to a large number of neuroendocrine peptides, known and novel, in a variety of regulatory paradigms...
  28. ncbi request reprint Obesity and diabetes in transgenic mice expressing proSAAS
    Suwen Wei
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    J Endocrinol 180:357-68. 2004
    ..Taken together, these results are consistent with a role for proSAAS-derived peptides as neuropeptides that influence body weight independently of their function as inhibitors of prohormone convertase 1...
  29. pmc Individual carboxypeptidase D domains have both redundant and unique functions in Drosophila development and behavior
    Galyna Sidyelyeva
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cell Mol Life Sci 67:2991-3004. 2010
    ..These findings suggest that CPD domains-1 and -2 have largely redundant functions in the processing of growth factors, hormones, and neuropeptides...
  30. pmc Analysis of peptides in prohormone convertase 1/3 null mouse brain using quantitative peptidomics
    Jonathan H Wardman
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurochem 114:215-25. 2010
    ..Analysis of these sites showed that while PC1/3 and PC2 have overlapping substrate preferences, there are particular cleavage site residues that distinguish peptides preferred by each PC...
  31. ncbi request reprint Characterization of the molecular basis of the Drosophila mutations in carboxypeptidase D. Effect on enzyme activity and expression
    Galyna Sidyelyeva
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 281:13844-52. 2006
    ..Flies that retain the short form are viable, whereas flies that are missing all forms of CPD do not survive past the early larval stages...
  32. ncbi request reprint Altered neuropeptide processing in prefrontal cortex of Cpe (fat/fat) mice: implications for neuropeptide discovery
    Jihyeon Lim
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurochem 96:1169-81. 2006
    ....
  33. ncbi request reprint ProSAAS and prohormone convertase 1 are broadly expressed during mouse development
    Yun Feng
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Brain Res Gene Expr Patterns 1:135-40. 2002
    ..In general, the expression pattern of PC1 overlaps with that of SAAS and other proSAAS-derived peptides, consistent with the hypothesis that proSAAS functions as an endogenous PC1 inhibitor...
  34. ncbi request reprint Examination of the rate of peptide biosynthesis in neuroendocrine cell lines using a stable isotopic label and mass spectrometry
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurochem 90:585-94. 2004
    ..These results are consistent with the acidic pH optima for peptide processing enzymes. Stable isotopic labeling with d10-Leu provides a sensitive method to examine the rate of peptide formation in neuroendocrine cell lines...
  35. pmc Processing of proSAAS in neuroendocrine cell lines
    Nino Mzhavia
    Department of Pharmacology, New York University School of Medicine, New York, NY 10016, U S A
    Biochem J 361:67-76. 2002
    ..In addition, the finding that the peptides are secreted via the regulated pathway is consistent with the proposal that they may function as neuropeptides...
  36. ncbi request reprint Characterization of Drosophila carboxypeptidase D
    Galyna Sidyelyeva
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Biol Chem 277:49613-20. 2002
    ..The differences in pH optima and substrate specificity between Drosophila domains 1B and 2 are similar to the differences between duck CPD domains 1 and 2, suggesting that these properties are essential to CPD function...
  37. ncbi request reprint Palmitoylation of carboxypeptidase D. Implications for intracellular trafficking
    Elena V Kalinina
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:9244-9. 2003
    ..Thus, the covalent attachment of palmitic acid to the Cys residues of CPD has a functional significance in the trafficking of the protein...
  38. ncbi request reprint Neuropeptide processing profile in mice lacking prohormone convertase-1
    Hui Pan
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, One Gustave L Levy Place, New York 10029, USA
    Biochemistry 44:4939-48. 2005
    ....
  39. ncbi request reprint Deficits in reproduction and pro-gonadotropin-releasing hormone processing in male Cpefat mice
    Sudha Srinivasan
    Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, North Carolina 27710, USA
    Endocrinology 145:2023-34. 2004
    ..Together, these findings show that CPE is a key enzyme for pro-GnRH processing in vivo; however, the reproductive deficits in Cpe(fat/fat) males appear to be due primarily to abnormal sexual behavior...
  40. ncbi request reprint Nna1-like proteins are active metallocarboxypeptidases of a new and diverse M14 subfamily
    Monica Rodriguez de la Vega
    Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 08193 Bellaterra Barcelona, Spain
    FASEB J 21:851-65. 2007
    ....
  41. ncbi request reprint A novel subfamily of mouse cytosolic carboxypeptidases
    Elena Kalinina
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    FASEB J 21:836-50. 2007
    ..Taken together, these results are consistent with a role for Nna1/CCP1 and the related CCPs in the processing of tubulin...
  42. ncbi request reprint Metallocarboxypeptidases: emerging drug targets in biomedicine
    Joan L Arolas
    Institut de Biotecnologia i Biomedicina and Dept de Bioquímica i Biologia Molecular, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
    Curr Pharm Des 13:349-66. 2007
    ..Future perspectives for the regulation of these enzymes through the use of more selective and potent inhibitors are also discussed in this review and combined with earlier observations in the field...
  43. ncbi request reprint Characterization of endothelin-converting enzyme-2. Implication for a role in the nonclassical processing of regulatory peptides
    Nino Mzhavia
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA
    J Biol Chem 278:14704-11. 2003
    ..Taken together, these results are consistent with an important role for ECE-2 in the processing of regulatory peptides at nonclassical sites...
  44. doi request reprint Characterization of carboxypeptidase A6, an extracellular matrix peptidase
    Peter J Lyons
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 283:7054-63. 2008
    ..Taken together, these data suggest a role for CPA6 in the regulation of neuropeptides in the extracellular environment within the olfactory bulb and other parts of the brain...
  45. ncbi request reprint Quantitative peptidomics in mice: effect of cocaine treatment
    Fa Yun Che
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Mol Neurosci 28:265-75. 2006
    ..Thirty other peptides were not substantially affected by cocaine treatment in both replicates. Taken together, the quantitative peptidomics approach provides an efficient method to screen for changes in a large number of peptides...
  46. ncbi request reprint Drosophila S2 cells produce multiple forms of carboxypeptidase D with different intracellular distributions
    Elena Kalinina
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Cell Biochem 99:770-83. 2006
    ....
  47. ncbi request reprint Analysis of the carboxypeptidase D cytoplasmic domain: Implications in intracellular trafficking
    Elena Kalinina
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Cell Biochem 85:101-11. 2002
    ..Taken together, these results suggest that CPD localization to the TGN requires both static retention involving the C-terminal domain and phosphorylation at a CK2 site, which regulates the binding of adaptor proteins...
  48. ncbi request reprint Identification and distribution of mouse carboxypeptidase A-6
    Jose D Fontenele-Neto
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Brain Res Mol Brain Res 137:132-42. 2005
    ..Taken together, the distribution of CPA6 suggests a specific role in a limited number of tissues, and it is possible that this role involves an aspect of cell migration...
  49. ncbi request reprint Identification and characterization of three members of the human metallocarboxypeptidase gene family
    Suwen Wei
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:14954-64. 2002
    ..The CP on chromosome 8 is predicted to have a CPA-like specificity for C-terminal hydrophobic residues and was named CPA6. The CP on chromosome 2 is predicted to cleave substrates with C-terminal acidic residues and was named CPO...

Research Grants41

  1. Neuropeptides, Processing Enzymes, and Drug Abuse
    Lloyd Fricker; Fiscal Year: 2009
    ....
  2. STRUCTURE/FUNCTION OF CARBOXYPEPTIDASE D
    Lloyd Fricker; Fiscal Year: 2003
    ..Together, this concerted approach will provide important information regarding the role of CPD in the production of neuroendocrine peptides. ..
  3. CELL BIOLOGY OF CARBOXYPEPTIDASE D
    Lloyd Fricker; Fiscal Year: 2003
    ..This will provide a better understanding of the proteins, either known or novel, that interact with CPD. These studies on CPD will advance our knowledge of protein trafficking in neuroendocrine cells. ..
  4. Quantitative Peptidomics of Food Deprivation
    Lloyd Fricker; Fiscal Year: 2005
    ..The studies in this initial R21 will provide an unbiased survey of neuropeptides that are altered by food deprivation, and which may therefore be involved in regulating food intake and/or body weight. ..
  5. Neuropeptides, Processing Enzymes, and Drug Abuse
    Lloyd Fricker; Fiscal Year: 2007
    ....
  6. Function of Carboxypeptidase D
    Lloyd Fricker; Fiscal Year: 2007
    ..The overall goal of this proposal is to understand the role of each domain within CPD, using a simple organism for which CPD mutants exist and which is amenable to transgenic analysis. ..
  7. ProSAAS,A Novel Peptide Substrate of Carboxypeptidase E
    Lloyd Fricker; Fiscal Year: 2005
    ....
  8. Peptidomics of Cocaine and Amphetamine Abuse
    Lloyd Fricker; Fiscal Year: 2005
    ..Taken together, these studies will provide a better understanding of the regulation of neuropeptides, both known and novel, in drug abuse. ..
  9. REGULATION OF NEUROPEPTIDE PROCESSING ENZYMES
    Lloyd Fricker; Fiscal Year: 2002
    ..The results of these studies will provide a better understanding of the enzymes involved with peptide biosynthesis. ..
  10. CELL BIOLOGY OF CARBOXYPEPTIDASE E/ENKEPHALIN CONVERTASE
    Lloyd Fricker; Fiscal Year: 1999
    ..Together, these complementary studies will provide important information on CPE, a key enzyme in the production of enkephalins and other molecules that are relevant to the biochemistry of drug abuse. ..
  11. REGULATION OF CARBOXYPEPTIDASE E (ENKEPHALIN CONVERTASE)
    Lloyd Fricker; Fiscal Year: 1993
    ..The results of this multi-level analysis will provide a better understanding of the mechanisms responsible for the regulation of CPE expression and activity...