Alan Finkelstein

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. pmc Proton-coupled protein transport through the anthrax toxin channel
    Alan Finkelstein
    Departments of Physiology and Biophysics and Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Philos Trans R Soc Lond B Biol Sci 364:209-15. 2009
  2. pmc TonB-dependent transporter FhuA in planar lipid bilayers: partial exit of its plug from the barrel
    Eshwar Udho
    Deptartment of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochemistry 51:6753-9. 2012
  3. pmc A kinetic analysis of protein transport through the anthrax toxin channel
    Daniel Basilio
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 137:521-31. 2011
  4. pmc Trapping a translocating protein within the anthrax toxin channel: implications for the secondary structure of permeating proteins
    Daniel Basilio
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 137:343-56. 2011
  5. pmc The colicin Ia receptor, Cir, is also the translocator for colicin Ia
    Karen S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Microbiol 75:567-78. 2010
  6. pmc Identification of channel-lining amino acid residues in the hydrophobic segment of colicin Ia
    Paul K Kienker
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 132:693-707. 2008
  7. pmc Evidence for a proton-protein symport mechanism in the anthrax toxin channel
    Daniel Basilio
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 133:307-14. 2009
  8. pmc Protein translocation by bacterial toxin channels: a comparison of diphtheria toxin and colicin Ia
    Zhengyan Wu
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biophys J 91:3249-56. 2006
  9. pmc Sizing the protein translocation pathway of colicin Ia channels
    Paul K Kienker
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Gen Physiol 122:161-76. 2003
  10. doi request reprint Anomalous proton selectivity in a large channel: colicin A
    Stephen L Slatin
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 47:1778-88. 2008

Research Grants

Detail Information

Publications12

  1. pmc Proton-coupled protein transport through the anthrax toxin channel
    Alan Finkelstein
    Departments of Physiology and Biophysics and Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Philos Trans R Soc Lond B Biol Sci 364:209-15. 2009
    ..This paper discusses how the (PA63)7 channel both participates in the unfolding of LF and OF and functions in their translocation as a proton-protein symporter...
  2. pmc TonB-dependent transporter FhuA in planar lipid bilayers: partial exit of its plug from the barrel
    Eshwar Udho
    Deptartment of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochemistry 51:6753-9. 2012
    ..Negative staining electron microscopy showed that FhuA in a detergent solution formed vesicles, thus explaining why an osmotic gradient promoted the insertion of FhuA into membranes...
  3. pmc A kinetic analysis of protein transport through the anthrax toxin channel
    Daniel Basilio
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 137:521-31. 2011
    ..The cumulative distribution of first-passage times of the rod past the end of the channel displays S-shaped kinetics with a voltage dependence in agreement with experimental data...
  4. pmc Trapping a translocating protein within the anthrax toxin channel: implications for the secondary structure of permeating proteins
    Daniel Basilio
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 137:343-56. 2011
    ..e., the channel unfolds the secondary structure of the protein. We also show that the channel not only can translocate LF(N) in the normal direction but also can, at least partially, translocate LF(N) in the opposite direction...
  5. pmc The colicin Ia receptor, Cir, is also the translocator for colicin Ia
    Karen S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Microbiol 75:567-78. 2010
    ..We show that purified T domain competes with Ia and protects E. coli from being killed by it. Thus, in addition to binding to colicin Ia's receptor-binding domain, Cir also binds weakly to its translocation domain...
  6. pmc Identification of channel-lining amino acid residues in the hydrophobic segment of colicin Ia
    Paul K Kienker
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 132:693-707. 2008
    ....
  7. pmc Evidence for a proton-protein symport mechanism in the anthrax toxin channel
    Daniel Basilio
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 133:307-14. 2009
    ..We also find that a site that disfavors the entry of negatively charged residues into the (PA(63))(7) channel resides at or near its Phi-clamp, the ring of seven phenylalanines near the channel's entrance...
  8. pmc Protein translocation by bacterial toxin channels: a comparison of diphtheria toxin and colicin Ia
    Zhengyan Wu
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biophys J 91:3249-56. 2006
    ..This difference cannot be attributed to specific recognition of the A chain by diphtheria toxin's translocation pathway because the translocation pathway also accommodates folded myoglobin...
  9. pmc Sizing the protein translocation pathway of colicin Ia channels
    Paul K Kienker
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Gen Physiol 122:161-76. 2003
    ..Thus, the pathway for translocation is >/=26 A in diameter, or can stretch to this value. This is large enough for an alpha-helical hairpin to fit through...
  10. doi request reprint Anomalous proton selectivity in a large channel: colicin A
    Stephen L Slatin
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 47:1778-88. 2008
    ..We are thus compelled to propose that an unorthodox mechanism is at work in this protein...
  11. pmc Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes
    Eshwar Udho
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 106:21990-5. 2009
    ..We hypothesize that 4 M urea reversibly unfolds the FhuA and Cir plugs, thereby opening an ion-conducting pathway through these channels, and that this mimics to some extent the in vivo action of TonB on these plugs...
  12. ncbi request reprint PA63 channel of anthrax toxin: an extended beta-barrel
    Shilla Nassi
    Department of Neuroscience and Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 41:1445-50. 2002
    ..The implication is that major rearrangement of domains in the prepore cap region is required for membrane insertion of the beta-barrel stem...

Research Grants19

  1. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 2007
    ..In addition, the development of agents that can block the PA63 channel of anthrax toxin could prove useful as a defense against this potential terrorist weapon. ..
  2. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 1993
    ....
  3. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 1990
    ..An understanding of the structure of these channels and the mechanism(s) of their voltage control can lead to a more rational understanding and treatment of the myriad of neurological and cardiac maladies that aflict mankind...
  4. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 2010
    ....