STEVEN ALMO

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. ncbi request reprint Structural genomics of protein phosphatases
    Steven C Almo
    Albert Einstein College of Medicine, Bronx, NY, USA
    J Struct Funct Genomics 8:121-40. 2007
  2. ncbi request reprint Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches
    Boris V Strokopytov
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Acta Crystallogr D Biol Crystallogr 61:285-93. 2005
  3. ncbi request reprint Structure of the actin crosslinking core of fimbrin
    Michael G Klein
    Department of Biochemistry, Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Structure 12:999-1013. 2004
  4. pmc Antibodies against immunodominant antigens of Mycobacterium tuberculosis in subjects with suspected tuberculosis in the United States compared by HIV status
    Jacqueline M Achkar
    Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Clin Vaccine Immunol 17:384-92. 2010
  5. pmc Structural genomics: a pipeline for providing structures for the biologist
    Mark R Chance
    Center for Synchrotron Biosciences, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Protein Sci 11:723-38. 2002
  6. ncbi request reprint Picomolar transition state analogue inhibitors of human 5'-methylthioadenosine phosphorylase and X-ray structure with MT-immucillin-A
    Vipender Singh
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochemistry 43:9-18. 2004
  7. ncbi request reprint Crystal structure of the Yersinia protein-tyrosine phosphatase YopH complexed with a specific small molecule inhibitor
    Jin Peng Sun
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:33392-9. 2003
  8. ncbi request reprint Anopheles gambiae purine nucleoside phosphorylase: catalysis, structure, and inhibition
    Erika A Taylor
    Department of Biochemistry, Albert Einstein College of Medicine at Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 46:12405-15. 2007
  9. ncbi request reprint Crystal structure of the actin binding domain of the cyclase-associated protein
    Tetyana Dodatko
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 43:10628-41. 2004
  10. ncbi request reprint T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface
    Erhu Cao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Immunity 26:311-21. 2007

Research Grants

  1. STRUCTURAL BIOLOGY OF THE ALLERGIC RESPONSE
    STEVEN ALMO; Fiscal Year: 2002
  2. PROFILIN--STRUCTURE/FUNCTION RELATIONSHIPS
    STEVEN ALMO; Fiscal Year: 1999
  3. STRUCTURAL BIOLOGY OF CYTOSKELETAL DYNAMICS
    STEVEN ALMO; Fiscal Year: 2003

Collaborators

Detail Information

Publications82

  1. ncbi request reprint Structural genomics of protein phosphatases
    Steven C Almo
    Albert Einstein College of Medicine, Bronx, NY, USA
    J Struct Funct Genomics 8:121-40. 2007
    ....
  2. ncbi request reprint Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches
    Boris V Strokopytov
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Acta Crystallogr D Biol Crystallogr 61:285-93. 2005
    ..The fully automated web-based implementation of this phased translation function is described...
  3. ncbi request reprint Structure of the actin crosslinking core of fimbrin
    Michael G Klein
    Department of Biochemistry, Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Structure 12:999-1013. 2004
    ..These results underscore the power of jointly considering structural and genetic suppressor data for obtaining unexpected and biologically relevant mechanistic information...
  4. pmc Antibodies against immunodominant antigens of Mycobacterium tuberculosis in subjects with suspected tuberculosis in the United States compared by HIV status
    Jacqueline M Achkar
    Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Clin Vaccine Immunol 17:384-92. 2010
    ..0; 95% confidence interval, 2.3 to 51.2; P = 0.002). These findings have implications for the adjunctive use of TB serodiagnosis with these antigens in HIV(+) subjects...
  5. pmc Structural genomics: a pipeline for providing structures for the biologist
    Mark R Chance
    Center for Synchrotron Biosciences, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Protein Sci 11:723-38. 2002
  6. ncbi request reprint Picomolar transition state analogue inhibitors of human 5'-methylthioadenosine phosphorylase and X-ray structure with MT-immucillin-A
    Vipender Singh
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Biochemistry 43:9-18. 2004
    ..These slow-onset, tight-binding transition state analogue inhibitors are the most powerful reported for MTAP and have sufficient affinity to be useful in inhibiting the polyamine pathway...
  7. ncbi request reprint Crystal structure of the Yersinia protein-tyrosine phosphatase YopH complexed with a specific small molecule inhibitor
    Jin Peng Sun
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:33392-9. 2003
    ....
  8. ncbi request reprint Anopheles gambiae purine nucleoside phosphorylase: catalysis, structure, and inhibition
    Erika A Taylor
    Department of Biochemistry, Albert Einstein College of Medicine at Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 46:12405-15. 2007
    ..The distance from the N1' cation to the phosphate O4 anion is shorter in the AgPNP.DADMe-ImmH.PO4 complex than in HsPNP.DADMe-ImmH.SO4, offering one explanation for the stronger inhibitory effect of DADMe-ImmH for AgPNP...
  9. ncbi request reprint Crystal structure of the actin binding domain of the cyclase-associated protein
    Tetyana Dodatko
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 43:10628-41. 2004
    ..Thus, the unusual right-handed beta-helical fold present in C-CAP appears to support a wide range of biological functions...
  10. ncbi request reprint T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface
    Erhu Cao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Immunity 26:311-21. 2007
    ....
  11. ncbi request reprint Over-the-barrier transition state analogues and crystal structure with Mycobacterium tuberculosis purine nucleoside phosphorylase
    Andrzej Lewandowicz
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 42:6057-66. 2003
    ..This approach has resulted in the highest affinity transition state analogues known for MtPNP...
  12. pmc Crystal structure of the receptor-binding domain of human B7-2: insights into organization and signaling
    Xuewu Zhang
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 100:2586-91. 2003
    ....
  13. pmc Evolution of GITRL immune function: murine GITRL exhibits unique structural and biochemical properties within the TNF superfamily
    Kausik Chattopadhyay
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 105:635-40. 2008
    ..Taken together, the unique structural and biochemical behavior of mouse GITRL, along with the unusual domain organization of murine GITR, support a previously unrecognized mechanism for signaling within the TNF superfamily...
  14. pmc Sequence, structure, function, immunity: structural genomics of costimulation
    Kausik Chattopadhyay
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA
    Immunol Rev 229:356-86. 2009
    ..This integrated 'atoms-to-animals' paradigm provides a comprehensive approach for defining atomic and molecular mechanisms...
  15. ncbi request reprint Structure and biochemical properties of PRL-1, a phosphatase implicated in cell growth, differentiation, and tumor invasion
    Jin Peng Sun
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 44:12009-21. 2005
    ..Biochemical studies in solution and in the cell support a potential regulatory role of this intramolecular disulfide bond formation in response to reactive oxygen species such as H2O2...
  16. ncbi request reprint Closed site complexes of adenine phosphoribosyltransferase from Giardia lamblia reveal a mechanism of ribosyl migration
    Wuxian Shi
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:39981-8. 2002
    ..1-A excursion of the ribosyl anomeric carbon, whereas the adenine ring and the 5-phosphoryl group remained fixed. G. lamblia APRTase therefore provides another example of nucleophilic displacement by electrophile migration...
  17. ncbi request reprint Radiation-induced site-specific damage of mercury derivatives: phasing and implications
    Udupi A Ramagopal
    Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY 10461, USA
    Acta Crystallogr D Biol Crystallogr 61:1289-98. 2005
    ..These results indicate that historically recalcitrant and newly emerging difficulties associated with Hg phasing should be revisited...
  18. pmc Crystal structure of the complex between programmed death-1 (PD-1) and its ligand PD-L2
    Eszter Lazar-Molnar
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 105:10483-8. 2008
    ..These studies define spatial and organizational constraints that control the localization and signaling of PD-1/PD-L complexes within the immunological synapse and provide a basis for manipulating the PD-1 pathways for immunotherapy...
  19. pmc Structure of CD84 provides insight into SLAM family function
    Qingrong Yan
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 104:10583-8. 2007
    ..This common molecular dimension provides an opportunity for all two-domain SLAM family receptors to colocalize within the immunological synapse and bridge the T cell and antigen-presenting cell...
  20. pmc Structural basis for the coevolution of a viral RNA-protein complex
    Jeffrey A Chao
    Department of Anatomy, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, New York 10461, USA
    Nat Struct Mol Biol 15:103-5. 2008
    ....
  21. pmc Neighboring group participation in the transition state of human purine nucleoside phosphorylase
    Andrew S Murkin
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 46:5038-49. 2007
    ..These surprising results establish that forces in the Michaelis complex, reported by the BIEs, can be reversed or enhanced at the transition state...
  22. ncbi request reprint Acid-induced equilibrium folding intermediate of human platelet profilin
    Glendon D McLachlan
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 46:6931-43. 2007
    ..Our findings suggest that a novel mechanism for pH induced disruption of the profilin-G-actin complex involve a nativelike unfolding intermediate of profilin...
  23. pmc Inhibition and structure of Trichomonas vaginalis purine nucleoside phosphorylase with picomolar transition state analogues
    Agnes Rinaldo-Matthis
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 46:659-68. 2007
    ..And this difference is explained by isotope-edited difference infrared spectroscopy with [6-18O]ImmH to establish that O6 is the keto tautomer in TvPNP x ImmH x PO4, causing an unfavorable leaving-group interaction...
  24. pmc Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470
    John Jeff Alvarado
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Biochem Parasitol 168:158-67. 2009
    ..bieneusi MetAP2. Comparison of microsporidian MetAP2 structures with human MetAP2 provides insights into the design of inhibitors that might exhibit specificity for microsporidian MetAP2...
  25. ncbi request reprint NTB-A receptor crystal structure: insights into homophilic interactions in the signaling lymphocytic activation molecule receptor family
    Erhu Cao
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Immunity 25:559-70. 2006
    ....
  26. pmc Structure and inhibition of a quorum sensing target from Streptococcus pneumoniae
    Vipender Singh
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 45:12929-41. 2006
    ..coli enzyme. Catalytic site efficiency is primarily responsible for this difference since k(cat)/K(m) for S. pneumoniae MTAN is decreased 845-fold relative to that of E. coli MTAN...
  27. pmc Assembly and structural properties of glucocorticoid-induced TNF receptor ligand: Implications for function
    Kausik Chattopadhyay
    Departments of Microbiology and Immunology, Cell Biology, Biochemistry, Physiology and Biophysics, Medicine Division of Endocrinology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 104:19452-7. 2007
    ....
  28. pmc Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins
    Oksana M Subach
    Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY10461, USA
    Chem Biol 17:333-41. 2010
    ..We propose a chemical mechanism in which the DsRed-like chromophore is formed via the mTagBFP-like blue intermediate...
  29. pmc L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase
    Agnes Rinaldo-Matthis
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Am Chem Soc 130:842-4. 2008
    ....
  30. pmc Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large Stokes shift
    Kiryl D Piatkevich
    Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    J Am Chem Soc 132:10762-70. 2010
    ..These data suggest that different chromophores formed by distinct tripeptides in different environments can be rationally modified to yield RFPs with novel photochemical properties...
  31. pmc Structure of diethyl phosphate bound to the binuclear metal center of phosphotriesterase
    Jungwook Kim
    Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 47:9497-504. 2008
    ..Density functional theory (DFT) calculations support a reaction mechanism that utilizes the bridging hydroxide as the direct nucleophile in the hydrolysis of organophosphate esters by PTE...
  32. pmc 1.8 A structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cells
    Kausik Chattopadhyay
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Acta Crystallogr D Biol Crystallogr 65:434-9. 2009
    ..coli-expressed protein, thus conclusively demonstrating the novel quaternary structure assembly behavior of murine GITRL...
  33. pmc Biochemical and structural characterization of the human TL1A ectodomain
    Chenyang Zhan
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 48:7636-45. 2009
    ..In summary, these studies provide insights into the structure and function of TL1A and provide the basis for the rational manipulation of its interactions with cognate receptors...
  34. pmc Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states
    Fedor V Subach
    Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 106:21097-102. 2009
    ..The double bond extends the pi-conjugation between the phenolic ring of Tyr-67, the imidazolone, and the N-acylimine, resulting in the red fluorescent chromophore...
  35. ncbi request reprint Transition state model and mechanism of nucleophilic aromatic substitution reactions catalyzed by human glutathione S-transferase M1a-1a
    Yury Patskovsky
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 45:3852-62. 2006
    ....
  36. ncbi request reprint Structural analysis of H2-Db class I molecules containing two different allelic forms of the type 1 diabetes susceptibility factor beta-2 microglobulin: implications for the mechanism underlying variations in antigen presentation
    Matthew M Roden
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Immunol 43:1370-8. 2006
    ..These data suggest that other mechanisms, such as differential association of MHC-I in multiprotein complexes, are likely responsible for the effect of beta2m on T1D development...
  37. ncbi request reprint Mapping the G-actin binding surface of cofilin using synchrotron protein footprinting
    Jing Qu Guan
    Center for Synchrotron Biosciences, Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 41:5765-75. 2002
    ..These results are generally consistent with, and complementary to, the results of previous site-directed mutagenesis studies and extend our understanding of the G-actin binding surface of cofilin...
  38. pmc Visualizing the Ca2+-dependent activation of gelsolin by using synchrotron footprinting
    Janna G Kiselar
    Department of Physiology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 1602, USA
    Proc Natl Acad Sci U S A 100:3942-7. 2003
    ....
  39. ncbi request reprint Structural analysis of gelsolin using synchrotron protein footprinting
    Janna G Kiselar
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York, 10461 1602, USA
    Mol Cell Proteomics 2:1120-32. 2003
    ..The results demonstrate that radiolytic protein footprinting can provide detailed structural information on the conformational dynamics of ligand-induced structural changes, and the data provide a detailed model for gelsolin activation...
  40. ncbi request reprint Expression, refolding, purification, molecular characterization, crystallization, and preliminary X-ray analysis of the receptor binding domain of human B7-2
    Xuewu Zhang
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Protein Expr Purif 25:105-13. 2002
    ..Refolded and purified B7-2 was crystallized by the hanging-drop vapor diffusion method, allowing for the initiation of an X-ray crystallographic study...
  41. ncbi request reprint COHbC and COHbS crystallize in the R2 quaternary state at neutral pH in the presence of PEG 4000
    Larysa N Patskovska
    Department of Medicine Division of Hematology, Albert Einstein College of Medicine, Bronx, New York, USA
    Acta Crystallogr D Biol Crystallogr 61:566-73. 2005
    ..Solubility studies and modeling suggest that at neutral pH and low ionic strength the beta6 mutant hemoglobins crystallize (betaK6 > betaV6) as a result of more favorable lattice contacts...
  42. ncbi request reprint Structural mechanisms of costimulation
    Jean Claude D Schwartz
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Nat Immunol 3:427-34. 2002
    ..An examination of the signaling mechanisms within the costimulatory receptor-ligand family provides an excellent framework to consider the general principles that are relevant to cell surface receptor-mediated signaling events...
  43. ncbi request reprint How H13 histocompatibility peptides differing by a single methyl group and lacking conventional MHC binding anchor motifs determine self-nonself discrimination
    David A Ostrov
    Department of Biochemistry, Albert Einstein College of Medicine, 1600 Morris Boulevard, Bronx, NY 10461, USA
    J Immunol 168:283-9. 2002
    ..These results provide a structural explanation for a classical non-MHC-encoded H Ag, and they call into question the requirement for contact between anchor residues and the major MHC binding pockets in vaccine design...
  44. ncbi request reprint Structural reorganization of proteins revealed by radiolysis and mass spectrometry: G-actin solution structure is divalent cation dependent
    Jing Qu Guan
    Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 42:11992-2000. 2003
    ..These conformational variations for isolated Mg(2+)-G-actin provide a structural basis for its greater tendency to polymerize into filaments as compared to Ca(2+)-G-actin...
  45. pmc Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins
    Meng Chiao Ho
    Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 106:20276-81. 2009
    ..Catalytic forces originate primarily from leaving group activation evident in both RTA and SAP in complex with transition state analogues...
  46. ncbi request reprint Structural basis of inducible costimulator ligand costimulatory function: determination of the cell surface oligomeric state and functional mapping of the receptor binding site of the protein
    Kausik Chattopadhyay
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Immunol 177:3920-9. 2006
    ....
  47. ncbi request reprint Structural and kinetic characterization of Escherichia coli TadA, the wobble-specific tRNA deaminase
    Jungwook Kim
    Department of Biochemistry, Center for Synchrotron Biosciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 45:6407-16. 2006
    ..This work begins to define the chemical and structural determinants responsible for catalysis and substrate recognition and lays the foundation for detailed mechanistic analysis of this essential enzyme...
  48. ncbi request reprint Crystal structure of PTP1B complexed with a potent and selective bidentate inhibitor
    Jin Peng Sun
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 278:12406-14. 2003
    ....
  49. pmc A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT
    Emmanuel S Burgos
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 106:13748-53. 2009
    ....
  50. pmc Structure of Ca2+-bound S100A4 and its interaction with peptides derived from nonmuscle myosin-IIA
    Vladimir N Malashkevich
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 47:5111-26. 2008
    ..These studies provide the foundation for understanding S100A4 target recognition and may support the development of reagents that interfere with S100A4 function...
  51. pmc Programmed death-1 (PD-1)-deficient mice are extraordinarily sensitive to tuberculosis
    Eszter Lazar-Molnar
    Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 107:13402-7. 2010
    ..Overall, our studies show that the PD-1 pathway is required to control excessive inflammatory responses after M. tuberculosis infection in the lungs...
  52. ncbi request reprint Structure and dynamics of the actin filament
    Jing Qu Guan
    Center for Synchrotron Biosciences, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 44:3166-75. 2005
    ..K., and Schriemer, D.C. (2003) J. Mol. Biol. 334, 373-385], which also did not detect any burial of the H plug upon formation of filaments...
  53. pmc ZBP1 recognition of beta-actin zipcode induces RNA looping
    Jeffrey A Chao
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Genes Dev 24:148-58. 2010
    ..The RNA looping induced by ZBP1 binding provides a mechanism for specific recognition and may facilitate the assembly of post-transcriptional regulatory complexes by remodeling the bound transcript...
  54. ncbi request reprint Regulation of myosin-IIA assembly and Mts1 binding by heavy chain phosphorylation
    Natalya G Dulyaninova
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 44:6867-76. 2005
    ....
  55. ncbi request reprint Structure of mutant human carbonmonoxyhemoglobin C (betaE6K) at 2.0 A resolution
    John C Dewan
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Acta Crystallogr D Biol Crystallogr 58:2038-42. 2002
    ..BetaK6 NZ might also interact with the main-chain carbonyl O atom of betaH117 and the carboxylate group of betaE22 from a symmetry-related tetramer...
  56. pmc Phenothiazines inhibit S100A4 function by inducing protein oligomerization
    Vladimir N Malashkevich
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 107:8605-10. 2010
    ..Together these studies support a unique mode of inhibition in which phenothiazines disrupt the S100A4/myosin-IIA interaction by sequestering S100A4 via small molecule-induced oligomerization...
  57. ncbi request reprint Atomic dissection of the hydrogen bond network for transition-state analogue binding to purine nucleoside phosphorylase
    Greg A Kicska
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 41:14489-98. 2002
    ..Groups involved in leaving group activation and ribooxacarbenium ion stabilization are central to the H-bond network that provides transition-state stabilization and tight binding of the immucillins...
  58. pmc Four generations of transition-state analogues for human purine nucleoside phosphorylase
    Meng Chiao Ho
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 107:4805-12. 2010
    ..Multiple solutions in transition-state analogue design are available to convert the energy of catalytic rate enhancement to binding energy in human PNP...
  59. pmc Evolution of enzymatic activities in the enolase superfamily: L-rhamnonate dehydratase
    John F Rakus
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 47:9944-54. 2008
    ....
  60. ncbi request reprint Evolution of enzymatic activities in the enolase superfamily: D-tartrate dehydratase from Bradyrhizobium japonicum
    Wen Shan Yew
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 45:14598-608. 2006
    ....
  61. ncbi request reprint Identification of functional residues on Caenorhabditis elegans actin-interacting protein 1 (UNC-78) for disassembly of actin depolymerizing factor/cofilin-bound actin filaments
    Kurato Mohri
    Department of Pathology, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 279:31697-707. 2004
    ..These data support a model in which this conserved surface of AIP1 plays a direct role in enhancing fragmentation/depolymerization of ADF/cofilin-bound actin filaments but not in barbed end capping...
  62. ncbi request reprint Evolution of enzymatic activities in the enolase superfamily: D-Mannonate dehydratase from Novosphingobium aromaticivorans
    John F Rakus
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 S Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 46:12896-908. 2007
    ....
  63. pmc A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein
    Pieta K Mattila
    Program in Cellular Biotechnology, Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
    Mol Biol Cell 15:5158-71. 2004
    ....
  64. ncbi request reprint Evolution of enzymatic activities in the enolase superfamily: L-talarate/galactarate dehydratase from Salmonella typhimurium LT2
    Wen Shan Yew
    Departments of Biochemistry and Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 46:9564-77. 2007
    ..The promiscuous catalytic activities discovered for STM3697 highlight the evolutionary potential of a "conserved" active site architecture...
  65. pmc Structural and functional dissection of the Abp1 ADFH actin-binding domain reveals versatile in vivo adapter functions
    Omar Quintero-Monzon
    Department of Biology and Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, MA 02454, USA
    Mol Biol Cell 16:3128-39. 2005
    ..Together, our data provide the first structural and functional view of the Abp1-actin interaction and show that Abp1 has distinct cellular roles as an adapter, linking different sets of ligands for each function...
  66. ncbi request reprint Structural diversity within the mononuclear and binuclear active sites of N-acetyl-D-glucosamine-6-phosphate deacetylase
    Richard S Hall
    Department of Chemistry, P O Box 30012, Texas A and M University, College Station, Texas 77842 3012, USA
    Biochemistry 46:7953-62. 2007
    ..The phosphate group of the inhibitor attached to the hydroxyl at C-6 is ion paired with Arg-227 from the adjacent subunit. NagA from Thermotoga maritima was shown to require a single divalent cation for full catalytic activity...
  67. ncbi request reprint Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution
    Michael R Bubb
    Research Service, Malcom Randall Department of Veterans Affairs Medical Center and the University of Florida College of Medicine, Gainesville, Florida 32608, USA
    J Biol Chem 277:20999-1006. 2002
    ..These results provide a potential explanation for the paradoxical nucleation of actin filaments that have exclusively parallel subunits by a dimer containing antiparallel subunits...
  68. doi request reprint At the periphery of the amidohydrolase superfamily: Bh0493 from Bacillus halodurans catalyzes the isomerization of D-galacturonate to D-tagaturonate
    Tinh T Nguyen
    Department of Chemistry, P O Box 30012, Texas A and M University, College Station, Texas 77842 3012, USA
    Biochemistry 47:1194-206. 2008
    ....
  69. ncbi request reprint D-Ribulose 5-phosphate 3-epimerase: functional and structural relationships to members of the ribulose-phosphate binding (beta/alpha)8-barrel superfamily
    Julie Akana
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 S Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 45:2493-503. 2006
    ..Instead, this "superfamily" may result from assembly from smaller modules, including the conserved phosphate binding motif associated with the C-terminal (beta/alpha)(2)-quarter barrel...
  70. ncbi request reprint Functional synergy of actin filament cross-linking proteins
    Yiider Tseng
    Department of Chemical Engineering and Graduate Program in Molecular Biophysics, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 277:25609-16. 2002
    ..Our studies support a re-evaluation of the notion of functional redundancy among cytoskeletal regulatory proteins...
  71. ncbi request reprint Structural effects of cofilin on longitudinal contacts in F-actin
    Andrey A Bobkov
    Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    J Mol Biol 323:739-50. 2002
    ..This suggests that cofilin binding and the conformational effect on F-actin are not coupled tightly. Overall, this study provides solution evidence for the weakening of longitudinal, subdomain 2/1 contacts in F-actin by cofilin...
  72. pmc A CapG gain-of-function mutant reveals critical structural and functional determinants for actin filament severing
    Y Zhang
    Department of Medicine, University of Florida, Gainesville, 32610, USA
    EMBO J 25:4458-67. 2006
    ..We conclude that efficient severing utilizes two actin monomer-binding sites, and that the length of the WH2-containing segment is a critical functional determinant for severing...
  73. ncbi request reprint New York-Structural GenomiX Research Consortium (NYSGXRC): a large scale center for the protein structure initiative
    Jeffrey B Bonanno
    Structural GenomiX, Inc, 10505 Roselle Street, San Diego, CA 92121, USA
    J Struct Funct Genomics 6:225-32. 2005
    ..An analysis of current and projected consortium metrics reflects progress to date and delineates opportunities for further technology development...
  74. ncbi request reprint Evolution of enzymatic activities in the enolase superfamily: L-fuconate dehydratase from Xanthomonas campestris
    Wen Shan Yew
    Department of Biochemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 45:14582-97. 2006
    ..The functional promiscuity discovered for FucD highlights possible structural mechanisms for evolution of function in the enolase superfamily...
  75. ncbi request reprint Mapping the interaction of cofilin with subdomain 2 on actin
    Sabrina A Benchaar
    Department of Chemistry and Biochemistry, Molecular Biology Institute and Department of Biological Chemistry, University of California, Los Angeles, California 90095, USA
    Biochemistry 46:225-33. 2007
    ..Overall, these results demonstrate that the region around Gln41 (subdomain 2) is involved in a weak binding of cofilin to G-actin...
  76. ncbi request reprint How actin crosslinking and bundling proteins cooperate to generate an enhanced cell mechanical response
    Yiider Tseng
    Department of Chemical and Biomolecular Engineering and Program in Molecular Biophysics, The Johns Hopkins University, Baltimore, MD 21218, USA
    Biochem Biophys Res Commun 334:183-92. 2005
    ..Thus the combination of structural proteins with similar function can provide the cell with unique properties that are required for biologically optimal responses...
  77. pmc Visualizing Arp2/3 complex activation mediated by binding of ATP and WASp using structural mass spectrometry
    Janna G Kiselar
    Case Center for Proteomics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
    Proc Natl Acad Sci U S A 104:1552-7. 2007
    ....
  78. ncbi request reprint Mechanistic diversity in the RuBisCO superfamily: the "enolase" in the methionine salvage pathway in Geobacillus kaustophilus
    Heidi J Imker
    Departments of Biochemistry and Chemistry, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 46:4077-89. 2007
    ..e., coordination to an essential Mg2+, but the proton abstraction is catalyzed by a different general base...
  79. ncbi request reprint Cofilin (ADF) affects lateral contacts in F-actin
    Andrey A Bobkov
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 337:93-104. 2004
    ..Overall, this study provides solution evidence for the weakening of lateral interactions in F-actin by cofilin...
  80. pmc Structure-based activity prediction for an enzyme of unknown function
    Johannes C Hermann
    Department of Pharmaceutical Chemistry, University of California, San Francisco, MC 2550 1700 4th Street, San Francisco, California 94158 2330, USA
    Nature 448:775-9. 2007
    ..maritima in a previously uncharacterized SAH degradation pathway. Structure-based docking with high-energy forms of potential substrates may be a useful tool to annotate enzymes for function...
  81. pmc Structural basis for substrate specificity in phosphate binding (beta/alpha)8-barrels: D-allulose 6-phosphate 3-epimerase from Escherichia coli K-12
    Kui K Chan
    Departments of Biochemistry and Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA
    Biochemistry 47:9608-17. 2008
    ....
  82. ncbi request reprint Structural biology of cellular machines
    Wah Chiu
    National Center for Macromolecular Imaging and Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Trends Cell Biol 16:144-50. 2006
    ..The establishment of a database describing the structural and dynamic properties of protein assemblies will provide novel opportunities to define the molecular and atomic mechanisms controlling overall cell physiology...

Research Grants15

  1. STRUCTURAL BIOLOGY OF THE ALLERGIC RESPONSE
    STEVEN ALMO; Fiscal Year: 2002
    ....
  2. PROFILIN--STRUCTURE/FUNCTION RELATIONSHIPS
    STEVEN ALMO; Fiscal Year: 1999
    ..The expression studies will test the importance of specific profilin activities in vivo, and identify species-specific differences in cytoskeletal regulatory mechanisms. ..
  3. STRUCTURAL BIOLOGY OF CYTOSKELETAL DYNAMICS
    STEVEN ALMO; Fiscal Year: 2003
    ..3) The domain organization of the Ena/VASP/WASP family and the ligand interactions that control site specific actin filament assembly and the integration of these activities into the overall physiology of the cell. ..