D W S Wong

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. ncbi request reprint Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library
    Charles C Lee
    USDA ARS WRRC, 800 Buchanan St, Albany, CA 94710, USA
    Extremophiles 10:295-300. 2006
  2. ncbi request reprint Synergistic action of recombinant alpha-amylase and glucoamylase on the hydrolysis of starch granules
    D W S Wong
    Western Regional Research Center, U S Department of Agriculture ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Protein J 26:159-64. 2007
  3. doi request reprint Structure and action mechanism of ligninolytic enzymes
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Biochem Biotechnol 157:174-209. 2009
  4. ncbi request reprint Microassay for rapid screening of alpha-amylase activity
    D W Wong
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Agric Food Chem 48:4540-3. 2000
  5. doi request reprint A novel xyloglucan-specific endo-beta-1,4-glucanase: biochemical properties and inhibition studies
    Dominic D W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Microbiol Biotechnol 86:1463-71. 2010
  6. ncbi request reprint Functional cloning and expression of a novel Endo-alpha-1,5-L-arabinanase from a metagenomic library
    Dominic W S Wong
    Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
    Protein Pept Lett 16:1435-41. 2009
  7. doi request reprint Cloning and characterization of a novel exo-alpha-1,5-L-arabinanase gene and the enzyme
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Microbiol Biotechnol 79:941-9. 2008
  8. ncbi request reprint Enzymatic deconstruction of backbone structures of the ramified regions in pectins
    Dominic Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Protein J 27:30-42. 2008
  9. ncbi request reprint Feruloyl esterase: a key enzyme in biomass degradation
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Biochem Biotechnol 133:87-112. 2006
  10. ncbi request reprint Characterization of active Lentinula edodes glucoamylase expressed and secreted by Saccharomyces cerevisiae
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, California 94710, USA
    Protein J 24:455-63. 2005

Detail Information

Publications26

  1. ncbi request reprint Cloning and characterization of a cold-active xylanase enzyme from an environmental DNA library
    Charles C Lee
    USDA ARS WRRC, 800 Buchanan St, Albany, CA 94710, USA
    Extremophiles 10:295-300. 2006
    ..At 4 degrees C, the apparent K (m) was 3.7 mg/ml, and the apparent k (cat) was 123/s...
  2. ncbi request reprint Synergistic action of recombinant alpha-amylase and glucoamylase on the hydrolysis of starch granules
    D W S Wong
    Western Regional Research Center, U S Department of Agriculture ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Protein J 26:159-64. 2007
    ..Ca(++) enhanced the stability of the free enzymes at 50 degrees C incubation. Inhibition was observed with the addition of 10 mM Fe(++) or Cu(++), while Mg(++ )and EDTA had lesser effect...
  3. doi request reprint Structure and action mechanism of ligninolytic enzymes
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Biochem Biotechnol 157:174-209. 2009
    ..This review covers the chemical nature of lignin substrates and focuses on the biochemical properties, molecular structures, reaction mechanisms, and related structures/functions of these enzymes...
  4. ncbi request reprint Microassay for rapid screening of alpha-amylase activity
    D W Wong
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Agric Food Chem 48:4540-3. 2000
    ..A standard curve for the conversion of absorbance to enzyme activity units was constructed. The protocol developed was applied to monitoring the production of ultralow concentrations of recombinant barley alpha-amylase in yeast cells...
  5. doi request reprint A novel xyloglucan-specific endo-beta-1,4-glucanase: biochemical properties and inhibition studies
    Dominic D W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Microbiol Biotechnol 86:1463-71. 2010
    ..The addition of XXXG resulted in linear noncompetitive inhibition on the hydrolysis of tamarind xyloglucan giving a K (i) of 1.46 +/- 0.13 mM. The enzyme was devoid of transglycosylase activities...
  6. ncbi request reprint Functional cloning and expression of a novel Endo-alpha-1,5-L-arabinanase from a metagenomic library
    Dominic W S Wong
    Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
    Protein Pept Lett 16:1435-41. 2009
    ..The pattern of degradation revealed an endo-acting mechanism with arabinotriose as the end product...
  7. doi request reprint Cloning and characterization of a novel exo-alpha-1,5-L-arabinanase gene and the enzyme
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Microbiol Biotechnol 79:941-9. 2008
    ..Multiple sequence alignment and homology modeling revealed unique short sequences of amino acids extending the loop involved in partial blocking of one end of the substrate-binding site on the surface of the molecule...
  8. ncbi request reprint Enzymatic deconstruction of backbone structures of the ramified regions in pectins
    Dominic Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Protein J 27:30-42. 2008
    ..Applications of these enzymes in fruit juice processing and in the production of bioactive compounds, as well as their technological relevance to the deconstruction of cell wall structures for biomass conversion are discussed...
  9. ncbi request reprint Feruloyl esterase: a key enzyme in biomass degradation
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Biochem Biotechnol 133:87-112. 2006
    ..The nature of the enzyme substrates and products, the role of synergistic interactions with xylanases and other accessory enzymes, as well as the sequence-structure relating to the reaction mechanism are emphasized...
  10. ncbi request reprint Characterization of active Lentinula edodes glucoamylase expressed and secreted by Saccharomyces cerevisiae
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, California 94710, USA
    Protein J 24:455-63. 2005
    ..Apparent Km and Vmax for soluble starch were estimated to be 3.0 mg/ml and 0.13 mg/ml/min (40 degrees C, pH 5.3), with an apparent kcat of 2.9 x 10(5) min(-1)...
  11. ncbi request reprint Cloning and characterization of an exo-xylogucanase from rumenal microbial metagenome
    Dominic D W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    Protein Pept Lett 17:803-8. 2010
    ..The hydrolysis of tamarind xyloglucan followed the Michaelis-Menten kinetics, yielding K(m) and V(max) of 2.12+/-0.13 mg/ml and 0.17+/-0.01 mg/ml/min (37 degrees C, pH 6.0), respectively...
  12. ncbi request reprint Isolation of a raw starch-binding fragment from barley alpha-amylase
    D W Wong
    Western Regional Research Center, USDA ARS, Albany, California 94710, USA
    J Protein Chem 19:373-7. 2000
    ..The fragment would be located at the C-terminal region, forming a majority of the antiparallel beta-sheets in domain C and the alpha7- and alpha8-helices of the (alpha/beta)8 domain...
  13. ncbi request reprint Increased expression and secretion of recombinant alpha-amylase in Saccharomyces cerevisiae by using glycerol as the carbon source
    Dominic W S Wong
    Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, California 94710, USA
    J Protein Chem 21:419-25. 2002
    ..This investigation demonstrates that the use of glycerol as a carbon source for S. cerevisiae enhances the synthesis and secretion of the recombinant enzyme while suppressing cell growth...
  14. ncbi request reprint Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch hydrolysis
    Dominic W S Wong
    Western Regional Research Center, USDA Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA
    Protein Pept Lett 10:459-68. 2003
    ..Active clones could be separated into two groups, based on high total activity or high specific activity...
  15. ncbi request reprint Applying combinatorial chemistry and biology to food research
    Dominic Wong
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
    J Agric Food Chem 52:7187-98. 2004
    ..This review describes the current techniques of combinatorial chemistry and their applications, with emphasis on examples in food science research...
  16. ncbi request reprint High-activity barley alpha-amylase by directed evolution
    Dominic W S Wong
    Western Regional Research Center, USDA Agricultural Research Service, Albany, CA 94710, USA
    Protein J 23:453-60. 2004
    ..Two of these mutations. Q44H and R303K, result in amino acids highly conserved in cereal alpha-amylases. R303K and F325Y are located in the raw starch-binding fragment of the enzyme molecule...
  17. ncbi request reprint Cloning and characterization of the xyn11A gene from Lentinula edodes
    Charles C Lee
    Western Regional Research Center, USDA ARS, Albany, CA 94710, USA
    Protein J 24:21-6. 2005
    ..5 mg/ml and a Vmax of 2.1 mmol/min/mg. Xyn11A produced primarily xylobiose, xylotriose, and xylotetraose from a birchwood xylan substrate. This is the first report on the cloning of a hemicellulase gene from L. edodes...
  18. ncbi request reprint Native or raw starch digestion: a key step in energy efficient biorefining of grain
    George H Robertson
    Western Regional Research Center, Agricultural Research Service Pacific West Area, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Agric Food Chem 54:353-65. 2006
    ..The discussion draws on resources found in enzymology, engineering, plant physiology, cereal chemistry, and kinetics...
  19. ncbi request reprint Isolation and characterization of a cold-active xylanase enzyme from Flavobacterium sp
    Charles C Lee
    United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan St, Albany, CA 94710, USA
    Curr Microbiol 52:112-6. 2006
    ..The enzyme has an apparent Km of 1.8 mg/ml and kcat of 100 sec-1 for beechwood xylan, attacks highly branched native xylan substrates, and does not have activity against glucans...
  20. doi request reprint An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening
    Charles C Lee
    USDA ARS, Albany, CA 94710, USA
    Appl Biochem Biotechnol 155:314-20. 2009
    ..We have developed an assay with commercially available reagents that can be used to search DNA libraries for alpha-glucuronidase genes in a high-throughput, solid phase activity screen...
  21. doi request reprint Purification and characterization of a glycoside hydrolase family 43 beta-xylosidase from Geobacillus thermoleovorans IT-08
    Kurt Wagschal
    Western Regional Research Center, USDA Agricultural Research Service, Albany, CA 94710, USA
    Appl Biochem Biotechnol 155:304-13. 2009
    ..5 degrees C. GbtXyl43A showed a broad substrate specificity for hydrolysis of xylooligosaccharides up to the highest degree of polymerization tested (xylopentaose), and also released xylose from birch and beechwood arabinoxylan...
  22. doi request reprint Biochemical characterization of a novel dual-function arabinofuranosidase/xylosidase isolated from a compost starter mixture
    Kurt Wagschal
    USDA Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Microbiol Biotechnol 81:855-63. 2009
    ..Thus, deAX can be classified as a dual-function xylosidase/arabinofuranosidase with respect to both artificial and natural substrate specificity...
  23. doi request reprint Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme
    Charles C Lee
    USDA ARS WRRC, 800 Buchanan St, Albany, CA 94710, USA
    Curr Microbiol 57:301-5. 2008
    ..The optimum activity of the enzyme was at pH 5-7 and 40-50 degrees C. The enzyme was stable at temperatures up to 50 degrees C. Against birchwood xylan, the enzyme had an apparent K(m) of 6.7 mg/mL and V(max) of 379 micromol/min/mg...
  24. ncbi request reprint An episomal expression vector for screening mutant gene libraries in Pichia pastoris
    Charles C Lee
    USDA ARS WRRC, 800 Buchanan St, Albany, CA 94710, USA
    Plasmid 54:80-5. 2005
    ..Expression of the genes is driven by the constitutive GAP promoter, thus eliminating the need for timed or cell density-specific inductions. The pBGP1 plasmid was used to screen a xylanase gene library to isolate higher activity mutants...
  25. doi request reprint Cloning, expression and characterization of a glycoside hydrolase family 39 xylosidase from Bacillus halodurans C-125
    Kurt Wagschal
    USDA Agricultural Research Service, Western Regional Research Center, Albany, CA 94710, USA
    Appl Biochem Biotechnol 146:69-78. 2008
    ..The enzyme was not inhibited by up to 200 mM xylose. The oligomerization state was tetrameric under the size-exclusion chromatography conditions employed...
  26. pmc Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates
    Kurt Wagschal
    Agricultural Research Service, U S Dept of Agriculture, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
    Appl Environ Microbiol 71:5318-23. 2005
    ..21 mM(-1) . s(-1). Nonlinear curve fitting to a substrate inhibition model showed that for xylotriose K(i) was 1.7 +/- 0.1 mM, k(cat) was 2.0 +/- 0.1 s(-1), K(m) was 0.144 +/- 0.011 mM, and k(cat)/K(m) was 14 +/- 1.3 mM(-1) . s(-1)...