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Species | Todd J WardSummaryAffiliation: Agricultural Research Service Country: USA Publications
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Publications
An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North AmericaTodd J Ward
Microbial Genomics and Bioprocessing Research Unit, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA
Fungal Genet Biol 45:473-84. 2008..The discovery of this previously unrecognized pathogen diversity has significant implications for food safety and cereal production in North America...
Multilocus genotyping assays for single nucleotide polymorphism-based subtyping of Listeria monocytogenes isolatesTodd J Ward
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, Peoria, IL 61604, USA
Appl Environ Microbiol 74:7629-42. 2008..These mutations were restricted to serogroup 1/2 isolates, which may explain the overrepresentation of serotype 4b isolates in human listeriosis cases...
Molecular and phenotypic characterization of Listeria monocytogenes from U.S. Department of Agriculture Food Safety and Inspection Service surveillance of ready-to-eat foods and processing facilitiesTodd J Ward
Bacterial Foodborne Pathogens and Mycology Research Unit, U S Department of Agriculture, Agricultural Research Service, Peoria, Illinois 61604, USA
J Food Prot 73:861-9. 2010..These results indicated that integration of PFGE and DNA sequence-based subtyping provides an improved framework for prediction of relative risk associated with L. monocytogenes strains from RTE foods...
A targeted multilocus genotyping assay for lineage, serogroup, and epidemic clone typing of Listeria monocytogenesTodd J Ward
Bacterial Foodborne Pathogens and Mycology Research Unit, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA
Appl Environ Microbiol 76:6680-4. 2010..The assay was designed to facilitate rapid strain characterization and the integration of subtype data into risk-based inspection programs...
A single-nucleotide-polymorphism-based multilocus genotyping assay for subtyping lineage I isolates of Listeria monocytogenesThomas F Ducey
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U S Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA
Appl Environ Microbiol 73:133-47. 2007..DNA sequences were deposited in the GenBank database under accession numbers DQ 812146 to DQ 812517, DQ 843664 to DQ 844598, and AY 512391 to AY 512502...
Multilocus genotyping and molecular phylogenetics resolve a novel head blight pathogen within the Fusarium graminearum species complex from EthiopiaKerry O'Donnell
Microbial Genomics Research Unit, National Center for Agricultural Utilization Research, U S Department of Agriculture, Agricultural Research Service, 1815 N University St, Peoria, IL 61604, USA
Fungal Genet Biol 45:1514-22. 2008..Herein the FHB pathogen from Ethiopia is formally described as a novel species...
Intraspecific phylogeny and lineage group identification based on the prfA virulence gene cluster of Listeria monocytogenesTodd J Ward
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U S Department of Agriculture, 1815 North University St, Peoria, IL 61604, USA
J Bacteriol 186:4994-5002. 2004..monocytogenes lineages may represent distinct species within the framework of the cohesion species concept...
Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum cladeKerry O'Donnell
National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA
Fungal Genet Biol 41:600-23. 2004..Species rank is formally proposed for the eight unnamed species within the Fg clade using fixed nucleotide characters...
Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversityDavid E Starkey
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, U S Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA
Fungal Genet Biol 44:1191-204. 2007..S. In addition, we describe a highly divergent population of F. graminearum from the Gulf Coast of the U.S., and divergent isolates of F. acaciae-mearnsii from Australia and South Africa...
Evolution of a large ribosomal RNA multigene family in filamentous fungi: birth and death of a concerted evolution paradigmAlejandro P Rooney
Microbial Genomics and Bioprocessing Research Unit, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA
Proc Natl Acad Sci U S A 102:5084-9. 2005..As such, we hypothesize that a mechanism resembling retroposition controls 5S rRNA gene amplification, dispersal, and integration in the genomes of filamentous fungi...
Birth-and-death evolution of the internalin multigene family in ListeriaAlejandro P Rooney
National Center for Agricultural Utilization Research, Agricultural Research Service, U S Department of Agriculture, 1815 N University Street, Peoria, IL 61604, USA
Gene 427:124-8. 2008....
Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytopathogenic FusariumTodd J Ward
Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, U S Department of Agriculture, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
Proc Natl Acad Sci U S A 99:9278-83. 2002..Chemotype-specific differences in selective constraint and evidence of adaptive evolution within trichothecene genes are also reported...
Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusariaKerry O'Donnell
Bacterial Foodborne Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
Fungal Genet Biol 52:20-31. 2013..Our results support two competing hypotheses for the circumscription of Fusarium and provide a framework for future comparative phylogenetic and genomic analyses of this agronomically and medically important genus...
Phylogenetic diversity and microsphere array-based genotyping of human pathogenic Fusaria, including isolates from the multistate contact lens-associated U.S. keratitis outbreaks of 2005 and 2006Kerry O'Donnell
Microbial Genomics and Bioprocessing Research Unit, Agricultural Research Service, U S Department of Agriculture, 1815 North University Street, Peoria, IL 61604 3999, USA
J Clin Microbiol 45:2235-48. 2007..The high degree of phylogenetic diversity represented among the corneal isolates is consistent with multiple sources of contamination...
The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specializationChristina A Cuomo
Broad Institute of the Massachusetts Institute of Technology and Harvard, Cambridge, MA 02142, USA
Science 317:1400-2. 2007..These regions of genome innovation may result from selection due to interactions of F. graminearum with its plant hosts...
The presence of GC-AG introns in Neurospora crassa and other euascomycetes determined from analyses of complete genomes: implications for automated gene predictionMartijn Rep
Plant Pathology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
Genomics 87:338-47. 2006..These findings have important implications for fungal genome annotation, as the automated annotations of euascomycete genomes incorrectly identified intron boundaries for all of the confirmed and probable GC-AG introns reported here...
Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from genomic DNAMonica K Borucki
USDA ARS, Animal Disease Research Unit, Pullman, WA 99164 6630, USA
J Clin Microbiol 43:3255-9. 2005..The ability to subtype genomic DNA without PCR amplification allows probes to be designed for many different regions within the bacterial genome and should allow high-resolution subtyping not possible with multiplex PCR...
The trichothecene biosynthesis gene cluster of Fusarium graminearum F15 contains a limited number of essential pathway genes and expressed non-essential genesMakoto Kimura
Laboratory for Remediation Research, Plant Science Center, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
FEBS Lett 539:105-10. 2003..In contrast, recombinant TRI8 protein displayed no C-3 deacetylase activity, suggesting that the loss or alteration of function contribute directly to the chemotype difference...
Conservation genomics: disequilibrium mapping of domestic cattle chromosomal segments in North American bison populationsNatalie D Halbert
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biochemical Sciences, Texas A and M University, College Station, TX 77843, USA
Mol Ecol 14:2343-62. 2005....
