Amy L Vincent

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. ncbi request reprint Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA 50010, USA
    Vet Microbiol 118:212-22. 2006
  2. doi request reprint A brief introduction to influenza A virus in swine
    Amy L Vincent
    Virus and Prion Research Unit, National Animal Disease Center, US Department of Agriculture, Agricultural Research Service, 1920 Dayton Avenue, Ames, IA, 50010, USA
    Methods Mol Biol 1161:243-58. 2014
  3. pmc Comparison of Human-Like H1 (δ-Cluster) Influenza A Viruses in the Swine Host
    Janice R Ciacci Zanella
    Virus and Prion Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA 50010, USA Labex USA, Brazilian Agriculture Research Corporation EMBRAPA, 70770 901 Brasília, DF, Brazil Embrapa Swine and Poultry Research Center, 89700 000 Concórdia, SC, Brazil
    Influenza Res Treat 2012:329029. 2012
  4. doi request reprint Kinetics of lung lesion development and pro-inflammatory cytokine response in pigs with vaccine-associated enhanced respiratory disease induced by challenge with pandemic (2009) A/H1N1 influenza virus
    P C Gauger
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    Vet Pathol 49:900-12. 2012
  5. pmc Live attenuated influenza vaccine provides superior protection from heterologous infection in pigs with maternal antibodies without inducing vaccine-associated enhanced respiratory disease
    Amy L Vincent
    Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa, USA
    J Virol 86:10597-605. 2012
  6. pmc Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, United States
    Vaccine 25:7999-8009. 2007
  7. doi request reprint Experimental inoculation of pigs with pandemic H1N1 2009 virus and HI cross-reactivity with contemporary swine influenza virus antisera
    Amy L Vincent
    Virus and Prion Diseases Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA, USA
    Influenza Other Respir Viruses 4:53-60. 2010
  8. ncbi request reprint Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccine
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2300 Dayton Road, Ames, IA 50010, USA
    Vet Microbiol 126:310-23. 2008
  9. doi request reprint Efficacy of inactivated swine influenza virus vaccines against the 2009 A/H1N1 influenza virus in pigs
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, 1920 Dayton Road, Ames, IA 50010, USA
    Vaccine 28:2782-7. 2010
  10. doi request reprint Characterization of an influenza A virus isolated from pigs during an outbreak of respiratory disease in swine and people during a county fair in the United States
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA
    Vet Microbiol 137:51-9. 2009

Detail Information

Publications51

  1. ncbi request reprint Evaluation of hemagglutinin subtype 1 swine influenza viruses from the United States
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA 50010, USA
    Vet Microbiol 118:212-22. 2006
    ..S. pig population exhibit considerable variability in pathogenicity. There appears to be an increase in genetic and antigenic diversity coincident with the emergence of the swine triple reassortant H3N2 in 1998...
  2. doi request reprint A brief introduction to influenza A virus in swine
    Amy L Vincent
    Virus and Prion Research Unit, National Animal Disease Center, US Department of Agriculture, Agricultural Research Service, 1920 Dayton Avenue, Ames, IA, 50010, USA
    Methods Mol Biol 1161:243-58. 2014
    ..The diversity of IAV in swine remains one of the critical challenges in diagnosis and control of this important pathogen for swine health, and in turn contributes to a significant public health risk. ..
  3. pmc Comparison of Human-Like H1 (δ-Cluster) Influenza A Viruses in the Swine Host
    Janice R Ciacci Zanella
    Virus and Prion Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA 50010, USA Labex USA, Brazilian Agriculture Research Corporation EMBRAPA, 70770 901 Brasília, DF, Brazil Embrapa Swine and Poultry Research Center, 89700 000 Concórdia, SC, Brazil
    Influenza Res Treat 2012:329029. 2012
    ..Greater serological cross-reactivity was observed for viruses from the same HA phylogenetic sub-cluster; however, antigenic differences between the sub-clusters may have implications for disease control strategies for pigs...
  4. doi request reprint Kinetics of lung lesion development and pro-inflammatory cytokine response in pigs with vaccine-associated enhanced respiratory disease induced by challenge with pandemic (2009) A/H1N1 influenza virus
    P C Gauger
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
    Vet Pathol 49:900-12. 2012
    ..The lung alterations and host immune response are consistent with the vaccine-associated enhanced respiratory disease (VAERD) clinical outcome observed reproducibly in this swine model...
  5. pmc Live attenuated influenza vaccine provides superior protection from heterologous infection in pigs with maternal antibodies without inducing vaccine-associated enhanced respiratory disease
    Amy L Vincent
    Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa, USA
    J Virol 86:10597-605. 2012
    ..These results have implications not only for pigs but also for other influenza virus host species...
  6. pmc Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, United States
    Vaccine 25:7999-8009. 2007
    ..However, there appeared to be substantial cross-reactivity in antibodies at the mucosal level with the CO99 virus in MLV vaccinated pigs...
  7. doi request reprint Experimental inoculation of pigs with pandemic H1N1 2009 virus and HI cross-reactivity with contemporary swine influenza virus antisera
    Amy L Vincent
    Virus and Prion Diseases Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA, USA
    Influenza Other Respir Viruses 4:53-60. 2010
    ..The gene constellation of the virus was a combination from swine influenza A viruses (SIV) of North American and Eurasian lineages that had never before been identified in swine or other species...
  8. ncbi request reprint Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccine
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2300 Dayton Road, Ames, IA 50010, USA
    Vet Microbiol 126:310-23. 2008
    ..In addition, live virus infection conferred protection against heterologous challenge...
  9. doi request reprint Efficacy of inactivated swine influenza virus vaccines against the 2009 A/H1N1 influenza virus in pigs
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, 1920 Dayton Road, Ames, IA 50010, USA
    Vaccine 28:2782-7. 2010
    ..However, consideration should be given for development of monovalent homologous vaccines to best protect the swine population thus limiting shedding and the potential transmission of 2009 A/H1N1 from pigs to people...
  10. doi request reprint Characterization of an influenza A virus isolated from pigs during an outbreak of respiratory disease in swine and people during a county fair in the United States
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA
    Vet Microbiol 137:51-9. 2009
    ..The characteristics of the OH07 virus in our pig experimental model as well as the documented human transmission warrant close monitoring of the spread of this virus in pig and human populations...
  11. pmc Absence of 2009 pandemic H1N1 influenza A virus in fresh pork
    Amy L Vincent
    Virus and Prion Diseases Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America
    PLoS ONE 4:e8367. 2009
    ..These findings support the WHO recommendation that pork harvested from pandemic influenza A H1N1 infected swine is safe to consume when following standard meat hygiene practices...
  12. doi request reprint Characterization of a newly emerged genetic cluster of H1N1 and H1N2 swine influenza virus in the United States
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, 2300 Dayton Road, Ames, IA 50010, USA
    Virus Genes 39:176-85. 2009
    ..This study indicates that the human-like H1 SIV can efficiently replicate and transmit in the swine host and now co-circulates with contemporary SIVs as a distinct genetic cluster of H1 SIV...
  13. doi request reprint Vaccine-associated enhanced respiratory disease does not interfere with the adaptive immune response following challenge with pandemic A/H1N1 2009
    Phillip C Gauger
    1 Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa
    Viral Immunol 26:314-21. 2013
    ..Thus, original antigenic sin does not appear to be a component of VAERD...
  14. pmc Efficacy in pigs of inactivated and live attenuated influenza virus vaccines against infection and transmission of an emerging H3N2 similar to the 2011-2012 H3N2v
    Crystal L Loving
    Virus and Prion Diseases Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, Iowa, USA
    J Virol 87:9895-903. 2013
    ....
  15. pmc Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine
    Jürgen A Richt
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2300 Dayton Ave B 15, Ames, IA 50010, USA
    J Virol 80:11009-18. 2006
    ..Vaccinated pigs were seronegative for NS1, indicating the potential use of the TX98 NS1black triangle126 MLV as a vaccine to differentiate infected from vaccinated animals...
  16. doi request reprint Enhanced pneumonia and disease in pigs vaccinated with an inactivated human-like (δ-cluster) H1N2 vaccine and challenged with pandemic 2009 H1N1 influenza virus
    Phillip C Gauger
    National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA
    Vaccine 29:2712-9. 2011
    ..Overall, these results suggest that inactivated SIV vaccines may potentiate clinical signs, inflammation and pneumonia following challenge with divergent homosubtypic viruses that do not share cross-reacting HI or SN antibodies...
  17. pmc Pathogenicity and transmission in pigs of the novel A(H3N2)v influenza virus isolated from humans and characterization of swine H3N2 viruses isolated in 2010-2011
    Pravina Kitikoon
    Virus and Prion Disease Research Unit, National Animal Disease Center, USDA ARS, Ames, Iowa, USA
    J Virol 86:6804-14. 2012
    ..Continued monitoring of these H3N2 viruses is necessary to evaluate the evolution and potential loss of population immunity in swine and humans...
  18. doi request reprint Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs
    Ratree Platt
    Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United States
    Vet Immunol Immunopathol 142:252-7. 2011
    ..Overall, the adjuvanted UV-inactivated A/SW/MN/02011/08 H1N2 SIV vaccine stimulated both humoral and cellular immune responses including the CD4-CD8+ T cell subset...
  19. pmc Identification of H2N3 influenza A viruses from swine in the United States
    Wenjun Ma
    Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Proc Natl Acad Sci U S A 104:20949-54. 2007
    ..Taken together, these findings suggest that the H2N3 virus has undergone some adaptation to the mammalian host and that their spread should be very closely monitored...
  20. doi request reprint Swine influenza virus vaccine serologic cross-reactivity to contemporary US swine H3N2 and efficacy in pigs infected with an H3N2 similar to 2011-2012 H3N2v
    Pravina Kitikoon
    Virus and Prion Research Unit, National Animal Disease Center, USDA ARS, Ames, IA, USA
    Influenza Other Respir Viruses 7:32-41. 2013
    ..An H3N2 variant (H3N2v) virus with the H1N1pdm09 matrix gene and the remaining genes of swine triple reassortant H3N2 caused outbreaks at agricultural fairs in 2011-2012...
  21. doi request reprint Genotype patterns of contemporary reassorted H3N2 virus in US swine
    Pravina Kitikoon
    Virus and Prion Disease Research Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    J Gen Virol 94:1236-41. 2013
    ..Mutations at putative antigenic sites as well as reduced serological cross-reactivity among the H3 subclusters suggest antigenic drift of these contemporary viruses...
  22. doi request reprint Identification and characterization of a highly virulent triple reassortant H1N1 swine influenza virus in the United States
    Wenjun Ma
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA
    Virus Genes 40:28-36. 2010
    ..The highly virulent H1N1 swine influenza virus is further evidence of reassortment among avian, human and swine influenza viruses and justifies the need for continued surveillance of influenza viruses in swine...
  23. doi request reprint Swine influenza viruses a North American perspective
    Amy L Vincent
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, Ames, Iowa 50010, USA
    Adv Virus Res 72:127-54. 2008
    ....
  24. doi request reprint Vaccination with NS1-truncated H3N2 swine influenza virus primes T cells and confers cross-protection against an H1N1 heterosubtypic challenge in pigs
    Matthew A Kappes
    Virus and Prion Diseases Research Unit, National Animal Disease Center, USDA ARS, 1920 Dayton Ave, PO Box 70, Ames, IA 50010, USA
    Vaccine 30:280-8. 2012
    ..These results demonstrate that NS1Δ SIV vaccines can elicit cell-mediated cross-protection against antigenically divergent strains...
  25. doi request reprint Polymorphisms in the haemagglutinin gene influenced the viral shedding of pandemic 2009 influenza virus in swine
    Alessio Lorusso
    Virus and Prion Research Unit, National Animal Disease Center, USDA ARS, Ames, IA, USA
    J Gen Virol 95:2618-26. 2014
    ..Relative increases of P186 in publicly available sequences from swine H1N1pdm09 viruses supported the experimental data, indicating this amino acid substitution conferred an advantage in swine. ..
  26. doi request reprint Differences in clinical disease and immune response of pigs challenged with a high-dose versus low-dose inoculum of porcine reproductive and respiratory syndrome virus
    Crystal L Loving
    Respiratory Diseases of Livestock Research Unit, U S Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010, USA
    Viral Immunol 21:315-25. 2008
    ..IFN-gamma may play a role in PRRSV pathogenesis, as serum levels increased significantly in pigs challenged with the high dose of PRRSV...
  27. pmc Intranasal vaccination with replication-defective adenovirus type 5 encoding influenza virus hemagglutinin elicits protective immunity to homologous challenge and partial protection to heterologous challenge in pigs
    Douglas R Braucher
    Virus and Prion Research Unit, National Animal Disease Center, United States Department of Agriculture Agricultural Research Service, Ames, Iowa, USA
    Clin Vaccine Immunol 19:1722-9. 2012
    ....
  28. pmc Utility of a panviral microarray for detection of swine respiratory viruses in clinical samples
    Tracy L Nicholson
    Virus and Prion Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA 50010, USA
    J Clin Microbiol 49:1542-8. 2011
    ....
  29. doi request reprint Vaccine efficacy and immune response to swine influenza virus challenge in pigs infected with porcine reproductive and respiratory syndrome virus at the time of SIV vaccination
    Pravina Kitikoon
    Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, P O Box 3020, Iowa State University, Ames, IA 50010, USA
    Vet Microbiol 139:235-44. 2009
    ..These findings demonstrate that PRRSV infection has a significant impact on SIV vaccine efficacy that may be important for disease control...
  30. doi request reprint Population dynamics of cocirculating swine influenza A viruses in the United States from 2009 to 2012
    Tavis K Anderson
    Virus and Prion Research Unit, National Animal Disease Center, USDA ARS, Ames, IA, USA
    Influenza Other Respir Viruses 7:42-51. 2013
    ..Recent advances in influenza surveillance in US swine populations allow for timely epidemiological, phylogenetic, and virological analyses that monitor emergence of novel viruses and assess changes in viral population dynamics...
  31. ncbi request reprint Contemporary epidemiology of North American lineage triple reassortant influenza A viruses in pigs
    Alessio Lorusso
    Virus and Prion Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA, USA
    Curr Top Microbiol Immunol 370:113-32. 2013
    ..The recent epidemiological data regarding the current state of influenza A virus subtypes circulating in the Canadian and American swine population is discussed in this review...
  32. pmc Genetic and antigenic characterization of H1 influenza viruses from United States swine from 2008
    Alessio Lorusso
    Virus and Prion Disease Research Unit, USDA ARS NADC, Ames, IA, USA
    J Gen Virol 92:919-30. 2011
    ..Serological cross-reactivity paired with antigenic cartography demonstrated that the viruses in the different phylogenetic clusters are also antigenically divergent...
  33. pmc Heightened adaptive immune responses following vaccination with a temperature-sensitive, live-attenuated influenza virus compared to adjuvanted, whole-inactivated virus in pigs
    Crystal L Loving
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, Ames, IA, United States
    Vaccine 30:5830-8. 2012
    ..Taken together, these results indicate the ts LAIV vaccine, generated from a triple reassortant IAV, elicits greater cell-mediated and humoral immune responses in pigs...
  34. doi request reprint Influenza virus coinfection with Bordetella bronchiseptica enhances bacterial colonization and host responses exacerbating pulmonary lesions
    Crystal L Loving
    Respiratory Diseases of Livestock Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA
    Microb Pathog 49:237-45. 2010
    ..Overall, Flu infection increased Bb colonization and enhanced production of proinflammatory mediators that likely contribute to exacerbated pulmonary lesions...
  35. ncbi request reprint Detection of anti-influenza A nucleoprotein antibodies in pigs using a commercial influenza epitope-blocking enzyme-linked immunosorbent assay developed for avian species
    Janice R Ciacci-Zanella
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, 1920 Dayton Road, Ames, IA 50010, USA
    J Vet Diagn Invest 22:3-9. 2010
    ..Overall, the assay showed excellent diagnostic performance against the range of influenza subtypes investigated and could serve as a useful screening assay for swine...
  36. doi request reprint Divergent immune responses and disease outcomes in piglets immunized with inactivated and attenuated H3N2 swine influenza vaccines in the presence of maternally-derived antibodies
    Matthew R Sandbulte
    Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA
    Virology 464:45-54. 2014
    ..Based on the data, one LAIV dose can induce cell-mediated immunity against antigenically divergent H3N2 influenza virus despite passive antibody interference with humoral immune responses. ..
  37. doi request reprint Live attenuated influenza A virus vaccine protects against A(H1N1)pdm09 heterologous challenge without vaccine associated enhanced respiratory disease
    Phillip C Gauger
    Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    Virology 471:93-104. 2014
    ..In contrast, infection-enhancing antibodies were not detected in the serum of LAIV vaccinates and VAERD was not observed. ..
  38. doi request reprint Comparative virulence of wild-type H1N1pdm09 influenza A isolates in swine
    Jamie N Henningson
    Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, Ames, IA 50010, USA Department of Diagnostic Medicine Pathobiology, College of Veterinary Medicine, Kansas State University, K 221 Mosier Hall, Manhattan, KS 66506, USA
    Vet Microbiol 176:40-9. 2015
    ..Importantly, the limited clinical signs in pigs could result in continued circulation of these viruses with other endemic swine IAVs providing opportunities for reassortment. ..
  39. doi request reprint Cross-fostering to prevent maternal cell transfer did not prevent vaccine-associated enhanced respiratory disease that occurred following heterologous influenza challenge of pigs vaccinated in the presence of maternal immunity
    Crystal L Loving
    1 Virus and Prion Diseases Research Unit, National Animal Disease Center, USDA ARS, Ames, Iowa
    Viral Immunol 27:334-42. 2014
    ..Future work is aimed at understanding how MDA alters WIV vaccine immunogenicity...
  40. pmc Novel swine influenza virus subtype H3N1, United States
    Porntippa Lekcharoensuk
    Virus and Prion Disease of Livestock Research Unit, National Animal Disease Center, US Department of Agriculture ARS, 2300 Dayton Avenue, Ames, IA 50010, USA
    Emerg Infect Dis 12:787-94. 2006
    ..Further surveillance will help determine whether this novel subtype will continue to circulate in swine populations...
  41. doi request reprint One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples
    Alessio Lorusso
    Virus and Prion Diseases Research Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    J Virol Methods 164:83-7. 2010
    ....
  42. doi request reprint Swine influenza matrix 2 (M2) protein contributes to protection against infection with different H1 swine influenza virus (SIV) isolates
    Pravina Kitikoon
    College of Veterinary Medicine, Iowa State University, Ames, IA, USA
    Vaccine 28:523-31. 2009
    ..This study found that the M2 protein has potential as a vaccine for SIV-associated disease prevention. However, development of an immune response towards the major envelope HA protein was required to reduce SIV shedding...
  43. doi request reprint Identification and molecular cloning of a novel porcine parvovirus
    Andrew K Cheung
    Virus and Prion Research Unit, National Animal Disease Center, PO Box 70, Ames, IA 50010, USA
    Arch Virol 155:801-6. 2010
    ..Whereas the ORF3-encoded proteins of bocaviruses share significant similarity with each other, the PPV4 ORF3 encoded protein does not exhibit homology with any protein in the GenBank non-redundant database...
  44. doi request reprint Influenza A virus hemagglutinin protein subunit vaccine elicits vaccine-associated enhanced respiratory disease in pigs
    Daniela S Rajão
    Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, PO Box 70, Ames, IA 50010, USA
    Vaccine 32:5170-6. 2014
    ..These results demonstrate that the immune response against the HA protein alone is sufficient to cause VAERD following heterologous challenge. ..
  45. doi request reprint Pathogenesis and vaccination of influenza A virus in swine
    Daniela S Rajão
    Virus and Prion Diseases of Livestock Research Unit, USDA ARS, 1920 Dayton Avenue, PO Box 70, Ames, IA, 50010, USA
    Curr Top Microbiol Immunol 385:307-26. 2014
    ....
  46. doi request reprint Antibody secreting cell assay for influenza A virus in swine
    Pravina Kitikoon
    Virus and Prion Research Unit, National Animal Disease Center, U S Department of Agriculture, Agricultural Research Service, 1920 Dayton Avenue, Ames, IA, 50010, USA
    Methods Mol Biol 1161:347-53. 2014
    ..The spot-forming cells are detected by enzyme labeled antibodies directed to the swine immunoglobulin (Ig) class of interest and visualized with the addition of substrate. ..
  47. doi request reprint Hemagglutinin inhibition assay with swine sera
    Pravina Kitikoon
    Virus and Prion Research Unit, US Department of Agriculture, National Animal Disease Center, Agricultural Research Service, Ames, IA, USA
    Methods Mol Biol 1161:295-301. 2014
    ..The hemagglutination inhibition (HI) test is a serological assay that measures the titer of specific antibodies in the sera and is the most common serological assay used to detect anti-influenza antibodies in swine sera. ..
  48. doi request reprint Enzyme-linked immunosorbent assay for detection of serum or mucosal isotype-specific IgG and IgA whole-virus antibody to influenza A virus in swine
    Phillip C Gauger
    Veterinary Diagnostic Laboratory, Iowa State University, 1600 South 16th Street, Ames, IA, 50011, USA
    Methods Mol Biol 1161:303-12. 2014
    ..The assay is useful to characterize the IgG or the IgA response to challenge or vaccination against specific influenza virus isolates in different compartments of the immune system. ..
  49. doi request reprint In vivo models for pathotyping and vaccine efficacy for swine influenza
    Kelly M Lager
    Virus and Prion Research Unit, National Animal Disease Center, U S Department of Agriculture, Agricultural Research Service, 1920 Dayton Avenue, Ames, IA, 50010, USA
    Methods Mol Biol 1161:355-61. 2014
    ..This chapter covers methods for experimental IAV infection of pigs and collection of specific samples to study the pathogenesis of swine influenza and vaccine efficacy. ..
  50. doi request reprint Microneutralization assay for swine influenza virus in swine serum
    Pravina Kitikoon
    Virus and Prion Research Unit, US Department of Agriculture, National Animal Disease Center, Agricultural Research Service, Ames, IA, USA
    Methods Mol Biol 1161:325-35. 2014
    ..The MN assay can be used to detect antibody from pigs with natural exposure or vaccination and can potentially be used to predict cross-protection between strains of influenza A virus. ..
  51. doi request reprint Serum virus neutralization assay for detection and quantitation of serum-neutralizing antibodies to influenza A virus in swine
    Phillip C Gauger
    Veterinary Diagnostic Laboratory, Iowa State University, 1600 South 16th Street, Ames, IA, 50011, USA
    Methods Mol Biol 1161:313-24. 2014
    ..The SVN test is useful to evaluate the level of serological cross-reactivity between vaccine antisera and variant influenza viruses that may correlate with cross-protection in the host. ..