Neil C Talbot

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. doi request reprint Growth and Development Symposium: Development, characterization, and use of a porcine epiblast-derived liver stem cell line: ARS-PICM-19
    N C Talbot
    USDA, ARS, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
    J Anim Sci 91:66-77. 2013
  2. doi request reprint Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells
    Neil C Talbot
    USDA, ARS, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 48:1-11. 2012
  3. ncbi request reprint Characterization of two subpopulations of the PICM-19 porcine liver stem cell line for use in cell-based extracorporeal liver assistance devices
    Neil C Talbot
    US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Maryland, USA
    Int J Artif Organs 33:348-61. 2010
  4. doi request reprint Feeder-independent continuous culture of the PICM-19 pig liver stem cell line
    Neil C Talbot
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Bldg 200, Rm 13, BARC East, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 46:746-57. 2010
  5. doi request reprint Proteomic analysis of the major cellular proteins of bovine trophectoderm cell lines derived from IVP, parthenogenetic and nuclear transfer embryos: Reduced expression of annexins I and II in nuclear transfer-derived cell lines
    Neil C Talbot
    U S Department of Agriculture, Agricultural Research Service USDA, ARS, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705 2350, United States
    Anim Reprod Sci 120:187-202. 2010
  6. doi request reprint The effects of space flight and microgravity on the growth and differentiation of PICM-19 pig liver stem cells
    Neil C Talbot
    USDA, ARS, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Bldg 200, Rm 13, BARC East, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 46:502-15. 2010
  7. ncbi request reprint Comparison of the interferon-tau expression from primary trophectoderm outgrowths derived from IVP, NT, and parthenogenote bovine blastocysts
    Neil C Talbot
    USDA, ARS, ANRI, Biotechnology and Germplasm Laboratory, Beltsville, Maryland 20705 2350, USA
    Mol Reprod Dev 75:299-308. 2008
  8. ncbi request reprint Establishment of a bovine blastocyst-derived cell line collection for the comparative analysis of embryos created in vivo and by in vitro fertilization, somatic cell nuclear transfer, or parthenogenetic activation
    Neil C Talbot
    USDA, ARS, ANRI, Biotechnology and Germplasm Laboratory, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 43:59-71. 2007
  9. ncbi request reprint Isolation and characterization of a bovine visceral endoderm cell line derived from a parthenogenetic blastocyst
    Neil C Talbot
    Agricultural Research Service, Animal and Natural Resources Institute, Biotechnology and Germplasm Laboratory, U S Department of Agriculture, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 41:130-41. 2005
  10. ncbi request reprint Spontaneous differentiation of porcine and bovine embryonic stem cells (epiblast) into astrocytes or neurons
    Neil C Talbot
    Gene Evaluation and Mapping Laboratory, Beltsville Agricultural Research Center, Building 200, Room 13, Maryland 20705, USA
    In Vitro Cell Dev Biol Anim 38:191-7. 2002

Detail Information

Publications22

  1. doi request reprint Growth and Development Symposium: Development, characterization, and use of a porcine epiblast-derived liver stem cell line: ARS-PICM-19
    N C Talbot
    USDA, ARS, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
    J Anim Sci 91:66-77. 2013
    ....
  2. doi request reprint Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells
    Neil C Talbot
    USDA, ARS, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 48:1-11. 2012
    ..The results may explain some of the cell growth and maintenance responses by various types of cells co-cultured on STO or CF-1 feeder cells...
  3. ncbi request reprint Characterization of two subpopulations of the PICM-19 porcine liver stem cell line for use in cell-based extracorporeal liver assistance devices
    Neil C Talbot
    US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Maryland, USA
    Int J Artif Organs 33:348-61. 2010
    ..PICM-19B cells produced markedly less serum proteins than PICM-19H cells. These data indicated that both cell lines, either together or alone, may be useful as the cellular substrate for an ALD...
  4. doi request reprint Feeder-independent continuous culture of the PICM-19 pig liver stem cell line
    Neil C Talbot
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Bldg 200, Rm 13, BARC East, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 46:746-57. 2010
    ....
  5. doi request reprint Proteomic analysis of the major cellular proteins of bovine trophectoderm cell lines derived from IVP, parthenogenetic and nuclear transfer embryos: Reduced expression of annexins I and II in nuclear transfer-derived cell lines
    Neil C Talbot
    U S Department of Agriculture, Agricultural Research Service USDA, ARS, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705 2350, United States
    Anim Reprod Sci 120:187-202. 2010
    ....
  6. doi request reprint The effects of space flight and microgravity on the growth and differentiation of PICM-19 pig liver stem cells
    Neil C Talbot
    USDA, ARS, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Bldg 200, Rm 13, BARC East, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 46:502-15. 2010
    ....
  7. ncbi request reprint Comparison of the interferon-tau expression from primary trophectoderm outgrowths derived from IVP, NT, and parthenogenote bovine blastocysts
    Neil C Talbot
    USDA, ARS, ANRI, Biotechnology and Germplasm Laboratory, Beltsville, Maryland 20705 2350, USA
    Mol Reprod Dev 75:299-308. 2008
    ..A differential expression of IFN-tau was also observed again, but this time as measured over time in culture. Maximal IFN-tau production was found at day-14 of primary culture and diminished to a minimum by the 23rd day...
  8. ncbi request reprint Establishment of a bovine blastocyst-derived cell line collection for the comparative analysis of embryos created in vivo and by in vitro fertilization, somatic cell nuclear transfer, or parthenogenetic activation
    Neil C Talbot
    USDA, ARS, ANRI, Biotechnology and Germplasm Laboratory, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 43:59-71. 2007
    ..The use of the trophectoderm cell lines as a comparative in vitro model of bovine trophectoderm and placental function is discussed in relation to NT reprogramming...
  9. ncbi request reprint Isolation and characterization of a bovine visceral endoderm cell line derived from a parthenogenetic blastocyst
    Neil C Talbot
    Agricultural Research Service, Animal and Natural Resources Institute, Biotechnology and Germplasm Laboratory, U S Department of Agriculture, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 41:130-41. 2005
    ..The cell line will be of interest for functional comparisons with bovine endoderm cell lines derived from IVF and nuclear transfer embryos...
  10. ncbi request reprint Spontaneous differentiation of porcine and bovine embryonic stem cells (epiblast) into astrocytes or neurons
    Neil C Talbot
    Gene Evaluation and Mapping Laboratory, Beltsville Agricultural Research Center, Building 200, Room 13, Maryland 20705, USA
    In Vitro Cell Dev Biol Anim 38:191-7. 2002
    ..The results confirm and extend the demonstrated in vitro pluripotency of porcine and bovine epiblast cultures and provide evidence for an in vitro model of embryonic neuroectoderm development...
  11. doi request reprint Culture of porcine hepatocytes or bile duct epithelial cells by inductive serum-free media
    Thomas J Caperna
    US Department of Agriculture, Agricultural Research Service, ANRI, Animal Biosciences and Biotechnology Laboratory, Bldg 200, Rm 201, BARC East, 10300 Baltimore Blvd, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 47:218-33. 2011
    ..The results demonstrate an in vitro model for the study of either porcine hepatocytes or cholangiocytes with in vivo-like morphology and function...
  12. doi request reprint Comparative proteomic and regulatory network analyses of the elongating pig conceptus
    Séverine A Degrelle
    USDA Agricultural Research Service, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville, MD, USA
    Proteomics 9:2678-94. 2009
    ..This proteomic study helps to elucidate the biological mechanisms underlying the early embryonic development of the pig...
  13. ncbi request reprint Expression analysis of pluripotency factors in the undifferentiated porcine inner cell mass and epiblast during in vitro culture
    Le Ann Blomberg
    Biotechnology and Germplasm Laboratory, USDA Agricultural Research Service, Beltsville, Maryland 20705, USA
    Mol Reprod Dev 75:450-63. 2008
    ..Our data demonstrate the potential use of some pluripotency factors as markers of porcine epiblast stem cells and indicate that the in vitro environment may influence the cultured epiblast's developmental state...
  14. doi request reprint The pursuit of ES cell lines of domesticated ungulates
    Neil C Talbot
    Animal Biosciences and Biotechnology Laboratory, ANRI, ARS, USDA, BARC East, Bldg 200, Rm 22, Beltsville, MD 20705, USA
    Stem Cell Rev 4:235-54. 2008
    ....
  15. ncbi request reprint Identification and characterization of aquaporin-9 (AQP9) in porcine hepatic tissue and hepatocytes in monolayer culture
    Thomas J Caperna
    Growth Biology Laboratory, Animal and Natural Resources Institute, USDA ARS, Beltsville Agricultural Research Center, USDA, Beltsville, MD 20705, United States
    Domest Anim Endocrinol 32:273-86. 2007
    ..Insulin alone had no discernable influence on AQP9 gene expression or its cellular protein levels. These data suggest that AQP9 is a major AQP in porcine hepatic tissue and appears to be primarily responsive to glucagon induction...
  16. ncbi request reprint Isolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst
    Neil C Talbot
    USDA, ARS, Animal and Natural Resources Institute, Biotechnology and Germplasm Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland, USA
    Mol Reprod Dev 69:164-73. 2004
    ..The cell line provides an in vitro model of parthenogenote trophectoderm whose biological characteristics can be compared to trophectoderm cell lines derived from bovine embryos produced by normal fertilization or nuclear transfer...
  17. doi request reprint Cytochrome P450 expression profile of the PICM-19H pig liver cell line: potential application to rapid liver toxicity assays
    Ryan R Willard
    USDA, ARS, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Bldg 200, Rm 13, BARC East, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 46:11-9. 2010
    ..59 μM and 31 μM, respectively. These results demonstrate the potential use of PICM-19H cells in drug biotransformation and toxicity testing and further support their use in extracorporeal artificial liver device technology...
  18. ncbi request reprint Isolation and characterization of porcine visceral endoderm cell lines derived from in vivo 11-day blastocysts
    Neil C Talbot
    Biotechnology and Germplasm Laboratory, Animal and Natural Resources Institute, ARS, USDA, Bldg 200, Rm 13, BARC East, Beltsville, MD 20705, USA
    In Vitro Cell Dev Biol Anim 43:72-86. 2007
    ..The cells have been grown for over 1 yr for multiple passages at 1:10 or 1:20 split ratios on STO feeder cells. The cell lines will be of interest as an in vitro model of the porcine preimplantation yolk-sac tissue...
  19. ncbi request reprint Analysis of the expression of aquaporin-1 and aquaporin-9 in pig liver tissue: comparison with rat liver tissue
    Neil C Talbot
    Gene Evaluation and Mapping Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
    Cells Tissues Organs 174:117-28. 2003
    ..The results indicate that specific AQPs are expressed in the tissues of the pig liver and that AQP-9 expression is distinct from its expression in the rat liver...
  20. ncbi request reprint The PICM-19 cell line as an in vitro model of liver bile ductules: effects of cAMP inducers, biopeptides and pH
    Neil C Talbot
    US Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
    Cells Tissues Organs 171:99-116. 2002
    ..The results indicated that the in vitro-produced ductal structures of the PICM-19 cell line are a functional model for biliary epithelium...
  21. ncbi request reprint Macrophage population dynamics within fetal mouse fibroblast cultures derived from C57BL/6, CD-1, CF-1 mice and interleukin-6 and granulocyte colony stimulating factor knockout mice
    Neil C Talbot
    U S Department of Agriculture, Agricultural Research Service, ANRI, Biotechnology and Germplasm Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland 20705, USA
    In Vitro Cell Dev Biol Anim 40:196-210. 2004
    ..Light and electron microscopy showed that the cultured macrophages had in vivo-like morphological features, and they could be plated to high purity by differential attachment to petri dishes in serum-free medium...
  22. ncbi request reprint Comparison of colony-formation efficiency of bovine fetal fibroblast cell lines cultured with low oxygen, hydrocortisone, L-carnosine, bFGF, or different levels of FBS
    Neil C Talbot
    USDA, ARS, Animal and Natural Resources Institute, Biotechnology and Germplasm Laboratory, Beltsville Agricultural Research Center, Maryland 20705, USA
    Cloning Stem Cells 6:37-47. 2004
    ..Also, some medium supplements or culture conditions that have shown positive CFE effects on the fibroblasts of other species failed to show significant positive CFE effects on the BFF cell lines tested...