Larry H Stanker

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. doi request reprint Development and partial characterization of high-affinity monoclonal antibodies for botulinum toxin type A and their use in analysis of milk by sandwich ELISA
    Larry H Stanker
    United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, United States
    J Immunol Methods 336:1-8. 2008
  2. doi request reprint Characterization of the epitope region of F1-2 and F1-5, two monoclonal antibodies to Botulinum neurotoxin type A
    Miles C Scotcher
    U S Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California 94710, USA
    Hybridoma (Larchmt) 28:315-25. 2009
  3. pmc Detection of botulinum neurotoxin serotype B at sub mouse LD(50) levels by a sandwich immunoassay and its application to toxin detection in milk
    Miles C Scotcher
    Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, California, USA
    PLoS ONE 5:e11047. 2010
  4. pmc Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain)
    Miles C Scotcher
    United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California, United States of America
    PLoS ONE 4:e4924. 2009
  5. doi request reprint Rapid and selective detection of botulinum neurotoxin serotype-A and -B with a single immunochromatographic test strip
    Kathryn H Ching
    United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan St, RM 2124, Albany, CA 94710, USA
    J Immunol Methods 380:23-9. 2012
  6. pmc Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum
    Luisa W Cheng
    Foodborne Contaminants Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, California 94710, United States
    J Agric Food Chem 61:755-60. 2013
  7. doi request reprint Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk
    Xiaohua He
    Western Regional Research Center, U S Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, United States
    J Immunol Methods 389:18-28. 2013
  8. doi request reprint Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay
    Reuven Rasooly
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, United States
    Int J Food Microbiol 126:135-9. 2008
  9. pmc The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri
    William G Miller
    Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, California, United States of America
    PLoS ONE 2:e1358. 2007
  10. ncbi request reprint Real-time quantitative PCR detection of Mycobacterium avium subsp. paratuberculosis and differentiation from other mycobacteria using SYBR Green and TaqMan assays
    Subbarao V Ravva
    United States Department of Agriculture, Agriculture Research Service, Western Regional Research Center, Foodborne Contaminants Research Unit, 800 Buchanan St, Albany, CA 94710, USA
    J Microbiol Methods 63:305-17. 2005

Detail Information

Publications16

  1. doi request reprint Development and partial characterization of high-affinity monoclonal antibodies for botulinum toxin type A and their use in analysis of milk by sandwich ELISA
    Larry H Stanker
    United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, United States
    J Immunol Methods 336:1-8. 2008
    ..A highly sensitive sandwich ELISA, capable of detecting as little as 2 pg/mL BoNT/A was developed using mAbs F1-2 and F1-40. Such an assay represents a realistic, high sensitivity alternative to the mouse bioassay...
  2. doi request reprint Characterization of the epitope region of F1-2 and F1-5, two monoclonal antibodies to Botulinum neurotoxin type A
    Miles C Scotcher
    U S Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California 94710, USA
    Hybridoma (Larchmt) 28:315-25. 2009
    ..The complementarity determining regions (CDRs) of the heavy chains were found to be 78% identical. It is possible that F1-2 and F1-5 bind the same epitope via the common amino acids within their CDRs...
  3. pmc Detection of botulinum neurotoxin serotype B at sub mouse LD(50) levels by a sandwich immunoassay and its application to toxin detection in milk
    Miles C Scotcher
    Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, California, USA
    PLoS ONE 5:e11047. 2010
    ..We previously reported production of high-affinity monoclonal antibodies to BoNT serotype A...
  4. pmc Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain)
    Miles C Scotcher
    United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, California, United States of America
    PLoS ONE 4:e4924. 2009
    ..We report the fine epitope mapping of F1-40 and the deduced amino acid sequence of the variable regions of the heavy and light chains of the antibody...
  5. doi request reprint Rapid and selective detection of botulinum neurotoxin serotype-A and -B with a single immunochromatographic test strip
    Kathryn H Ching
    United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan St, RM 2124, Albany, CA 94710, USA
    J Immunol Methods 380:23-9. 2012
    ..These attributes make this rapid diagnostic device a potentially valuable tool in the fields of food safety and homeland security...
  6. pmc Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum
    Luisa W Cheng
    Foodborne Contaminants Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, California 94710, United States
    J Agric Food Chem 61:755-60. 2013
    ..Both the ELISA and the ECL immunoassay platforms are fast, simple alternatives for use in the routine detection of BoNTs in food and animal sera...
  7. doi request reprint Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk
    Xiaohua He
    Western Regional Research Center, U S Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, United States
    J Immunol Methods 389:18-28. 2013
    ..01 pg/mL of Stx2a in milk, were developed using mAb pair Stx2-1 and Stx2-2. Such assays are useful for routine diagnosis of Stx2 contamination in milk production process, thus reducing the risk of STEC outbreaks...
  8. doi request reprint Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay
    Reuven Rasooly
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, United States
    Int J Food Microbiol 126:135-9. 2008
    ..This fast and effective assay system could be used for large scale screening to detect BoNT-A...
  9. pmc The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri
    William G Miller
    Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, California, United States of America
    PLoS ONE 2:e1358. 2007
    ..Here we present the complete genome sequence of the human clinical isolate, A. butzleri strain RM4018...
  10. ncbi request reprint Real-time quantitative PCR detection of Mycobacterium avium subsp. paratuberculosis and differentiation from other mycobacteria using SYBR Green and TaqMan assays
    Subbarao V Ravva
    United States Department of Agriculture, Agriculture Research Service, Western Regional Research Center, Foodborne Contaminants Research Unit, 800 Buchanan St, Albany, CA 94710, USA
    J Microbiol Methods 63:305-17. 2005
    ..No reporter signal was detected during TaqMan assays performed with 100 pg of template DNA from the non-Map organisms...
  11. pmc Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies
    Luisa W Cheng
    Western Regional Research Center, U S Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA
    Toxins (Basel) 5:1845-58. 2013
    ..The advent of better diagnositic methods and the availability of a greater arsenal of therapeutic mAbs against Stx2 would greatly enhance treatment outcomes of life threatening E. coli infections...
  12. pmc Conformation-dependent high-affinity monoclonal antibodies to prion proteins
    Larry H Stanker
    Foodborne Contaminants Research Unit, U S Department of Agriculture Agricultural Research Service, Albany, CA 94710, USA
    J Immunol 185:729-37. 2010
    ..These new conformation-dependent mAbs were found to be particularly useful in histoblotting studies, in which the low backgrounds after treatment with 2-ME created unusually high signal-to-noise ratios...
  13. pmc A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food
    Larry H Stanker
    Foodborne Toxin Detection and Prevention Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94510, USA
    Toxins (Basel) 5:2212-26. 2013
    ..Thus, the sandwich ELISA described here uses mAb for both the capture and detector antibodies (binding different epitopes on the toxin molecule) and readily detects toxin in those food samples tested. ..
  14. doi request reprint A rapid method to improve protein detection by indirect ELISA
    Robert Hnasko
    800 Buchanan St, USDA ARS, Western Regional Research Center, Albany, CA 94710, USA
    Biochem Biophys Res Commun 410:726-31. 2011
    ....
  15. pmc Antibody protection against botulinum neurotoxin intoxication in mice
    Luisa W Cheng
    Foodborne Contaminants Research Unit, Western Regional Research Center, USDA Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA
    Infect Immun 77:4305-13. 2009
    ..Knowledge of BoNT intoxication and antibody clearance in these mouse models and understanding of the pharmacokinetics of BoNT are invaluable for future development of antibodies and therapeutics against intoxication by BoNT...
  16. doi request reprint Effects of purification on the bioavailability of botulinum neurotoxin type A
    Luisa W Cheng
    Foodborne Contaminants Research Unit, Western Regional Research Center, USDA Agricultural Research Service, Albany, CA 94710, USA
    Toxicology 249:123-9. 2008
    ..Analysis of crude toxin composition permitted assessment of the impact of accessory proteins on the oral bioavailability of BoNT/A toxin in food matrices...