J R Stabel

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. pmc Immunoreactivity of the Mycobacterium avium subsp. paratuberculosis 19-kDa lipoprotein
    Jason F J Huntley
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    BMC Microbiol 5:3. 2005
  2. pmc Killing of Mycobacterium avium subspecies paratuberculosis within macrophages
    John P Bannantine
    National Animal Disease Center, ARS USDA, Ames, Iowa, USA
    BMC Microbiol 2:2. 2002
  3. pmc Heat inactivation of Mycobacterium paratuberculosis in raw milk: are current pasteurization conditions effective?
    J R Stabel
    National Animal Disease Center, U S Department of Agriculture, Ames, Iowa 50010, USA
    Appl Environ Microbiol 63:4975-7. 1997
  4. ncbi request reprint Immune responses after oral inoculation of weanling bison or beef calves with a bison or cattle isolate of Mycobacterium avium subsp. paratuberculosis
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, 2300 Dayton Road, Ames, Iowa 50010, USA
    J Wildl Dis 39:545-55. 2003
  5. ncbi request reprint Destruction of Mycobacterium paratuberculosis, Salmonella spp., and Mycoplasma spp. in raw milk by a commercial on-farm high-temperature, short-time pasteurizer
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50014, USA
    J Dairy Sci 87:2177-83. 2004
  6. ncbi request reprint Temporal Mycobacterium paratuberculosis infection in T-cell receptor (TCR)-alpha and TCR-delta-deficient mice
    J R Stabel
    National Disease of Livestock Research Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    Vet Immunol Immunopathol 89:127-32. 2002
  7. ncbi request reprint Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne's disease in US dairy herds
    J R Stabel
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    J Dairy Sci 85:525-31. 2002
  8. pmc Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50010, USA
    J Clin Microbiol 43:4744-50. 2005
  9. ncbi request reprint An evaluation of a modified interferon-gamma assay for the detection of paratuberculosis in dairy herds
    J R Stabel
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, 2300 Dayton Road, Ames, IA 50010, USA
    Vet Immunol Immunopathol 79:69-81. 2001
  10. ncbi request reprint Effects of supplemental energy on metabolic and immune measurements in periparturient dairy cows with Johne's disease
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50010, USA
    J Dairy Sci 86:3527-35. 2003

Detail Information

Publications67

  1. pmc Immunoreactivity of the Mycobacterium avium subsp. paratuberculosis 19-kDa lipoprotein
    Jason F J Huntley
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    BMC Microbiol 5:3. 2005
    ..paratuberculosis (M. avium subsp. paratuberculosis) 19-kDa lipoprotein as an immunomodulator in cattle with Johne's disease, the gene encoding the 19-kDa protein (MAP0261c) was analyzed...
  2. pmc Killing of Mycobacterium avium subspecies paratuberculosis within macrophages
    John P Bannantine
    National Animal Disease Center, ARS USDA, Ames, Iowa, USA
    BMC Microbiol 2:2. 2002
    ..We examined survival of M. paratuberculosis infections within cultured macrophages to better understand the interplay between bacterium and host...
  3. pmc Heat inactivation of Mycobacterium paratuberculosis in raw milk: are current pasteurization conditions effective?
    J R Stabel
    National Animal Disease Center, U S Department of Agriculture, Ames, Iowa 50010, USA
    Appl Environ Microbiol 63:4975-7. 1997
    ..Use of a laboratory-scale pasteurizer unit demonstrated that treatment of raw milk at 72 degrees C for 15 s effectively killed all M. paratuberculosis...
  4. ncbi request reprint Immune responses after oral inoculation of weanling bison or beef calves with a bison or cattle isolate of Mycobacterium avium subsp. paratuberculosis
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, 2300 Dayton Road, Ames, Iowa 50010, USA
    J Wildl Dis 39:545-55. 2003
    ..Antibody was not detected in either beef or bison calves during the 6 mo infection period. These results indicate that the host response to strains of M. avium subsp. paratuberculosis may differ between ruminant species...
  5. ncbi request reprint Destruction of Mycobacterium paratuberculosis, Salmonella spp., and Mycoplasma spp. in raw milk by a commercial on-farm high-temperature, short-time pasteurizer
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50014, USA
    J Dairy Sci 87:2177-83. 2004
    ..paratuberculosis but resulted in an average 25% reduction in colostral immunoglobulin. These results suggest that HTST pasteurization is effective in generating a safer product to feed to young calves...
  6. ncbi request reprint Temporal Mycobacterium paratuberculosis infection in T-cell receptor (TCR)-alpha and TCR-delta-deficient mice
    J R Stabel
    National Disease of Livestock Research Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    Vet Immunol Immunopathol 89:127-32. 2002
    ..These results suggest that alphabeta T cells play a major role in resistance to infection with M. paratuberculosis and that gammadelta T cells may play a lesser role and potentially confound protective immune responses...
  7. ncbi request reprint Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne's disease in US dairy herds
    J R Stabel
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    J Dairy Sci 85:525-31. 2002
    ..In addition, contamination of bulk tank milk samples with M. paratuberculosis does occur in seropositive herds, even in some with negative fecal cultures...
  8. pmc Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50010, USA
    J Clin Microbiol 43:4744-50. 2005
    ..In conclusion, the ISMap02 element provides a very sensitive and specific alternative as a diagnostic reagent for use in PCR assays for the detection of paratuberculosis...
  9. ncbi request reprint An evaluation of a modified interferon-gamma assay for the detection of paratuberculosis in dairy herds
    J R Stabel
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, 2300 Dayton Road, Ames, IA 50010, USA
    Vet Immunol Immunopathol 79:69-81. 2001
    ....
  10. ncbi request reprint Effects of supplemental energy on metabolic and immune measurements in periparturient dairy cows with Johne's disease
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50010, USA
    J Dairy Sci 86:3527-35. 2003
    ..These data demonstrate that energy balance is improved by providing additional energy in this manner and suggest energy supplementation can improve some aspects of immune function during the periparturient period...
  11. ncbi request reprint Augmentation of secreted and intracellular gamma interferon following johnin purified protein derivative sensitization of cows naturally infected with Mycobacterium avium subsp. paratuberculosis
    Judith R Stabel
    USDA ARS, National Animal Disease Center, 2300 Dayton Rd, Ames, IA 50010, USA
    J Vet Diagn Invest 19:43-51. 2007
    ..In addition, CD8+ lymphocytes appear to play an important role as a mediator of M. paratuberculosis infection in naturally exposed cattle...
  12. ncbi request reprint Dietary calcium modulates Mycobacterium paratuberculosis infection in beige mice
    J R Stabel
    Zoonotic Diseases Research Unit, NADC, ARS, USDA, Ames, IA 50010, USA
    Vet Immunol Immunopathol 66:377-90. 1998
    ..Numbers of viable bacteria cultured from the ileum and MLN after 6 months of infection were also significantly reduced in 1.0% Ca mice. These results suggest that Ca metabolism is an important modulator of M. paratuberculosis infection...
  13. ncbi request reprint Host responses to Mycobacterium avium subsp. paratuberculosis: a complex arsenal
    J R Stabel
    USDA ARS NADC, Bacterial Diseases of Livestock Research Unit, 2300 Dayton Rd, Ames, IA 50010, USA
    Anim Health Res Rev 7:61-70. 2006
    ..paratuberculosis (M. paratuberculosis), in an attempt to understand the dialogue between immune cells and their mediators during infection and what causes this discourse to go awry...
  14. ncbi request reprint Johne's disease and milk: do consumers need to worry?
    J R Stabel
    National Animal Disease Center, Ames, IA 50010, USA
    J Dairy Sci 83:1659-63. 2000
    ..A summary of past and current studies concerning this issue along with a discussion of methodologies used to recover M. paratuberculosis from experimentally inoculated milk will be presented in this paper...
  15. ncbi request reprint Efficacy of pasteurization conditions for the inactivation of Mycobacterium avium subsp. paratuberculosis in milk
    J R Stabel
    US Department of Agriculture, Animal Research Service, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, Iowa 50010, USA
    J Food Prot 67:2719-26. 2004
    ..paratuberculosis, but some bacteria survived subpasteurization heat treatment of milk used for cheese manufacture...
  16. ncbi request reprint Transitions in immune responses to Mycobacterium paratuberculosis
    J R Stabel
    USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    Vet Microbiol 77:465-73. 2000
    ..This paper reviews the current knowledge of T cell immune responses in M. paratuberculosis infection based upon clinical studies and research using mouse models...
  17. ncbi request reprint On-farm batch pasteurization destroys Mycobacterium paratuberculosis in waste milk
    J R Stabel
    USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    J Dairy Sci 84:524-7. 2001
    ..No viable bacteria were recovered after 28 wk of incubation. These results suggest that batch pasteurization of waste milk contaminated with M. paratuberculosis was effective at generating a clean product to feed to young calves...
  18. doi request reprint Pathogenesis of Mycobacterium avium subsp. paratuberculosis in neonatal calves after oral or intraperitoneal experimental infection
    J R Stabel
    USDA ARS, National Animal Disease Center, Ames, IA 50010, United States
    Vet Microbiol 136:306-13. 2009
    ..These data suggest that a variety of experimental infection regimes can be effective but oral inoculation with a clinical isolate may result in greater colonization of tissues and fecal shedding of M. avium subsp. paratuberculosis...
  19. doi request reprint Osteopontin: a novel cytokine involved in the regulation of Mycobacterium avium subspecies paratuberculosis infection in periparturient dairy cattle
    E L Karcher
    Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
    J Dairy Sci 91:3079-91. 2008
    ..This is the first study to evaluate the role of Opn on the immune response of dairy cows naturally infected with MAP, and results suggest Opn may be a key regulator against MAP infection...
  20. ncbi request reprint HspX is present within Mycobacterium paratuberculosis-infected macrophages and is recognized by sera from some infected cattle
    J P Bannantine
    National Animal Disease Center, ARS USDA, Ames, IA 50010, USA
    Vet Microbiol 76:343-58. 2000
    ..Collectively, these data give a preliminary characterization of the first described M. paratuberculosis protein identified within infected macrophages...
  21. pmc Early induction of humoral and cellular immune responses during experimental Mycobacterium avium subsp. paratuberculosis infection of calves
    W R Waters
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA
    Infect Immun 71:5130-8. 2003
    ..Additionally, this study confirms that mycobacteria-specific antibody is detectable early in the course of experimental Johne's disease, even preceding the development of specific cell-mediated responses...
  22. pmc Antigen-specific B-cell unresponsiveness induced by chronic Mycobacterium avium subsp. paratuberculosis infection of cattle
    W R Waters
    Veterinary Medical Research Institute, Iowa State University, Ames, Iowa 50011, USA
    Infect Immun 67:1593-8. 1999
    ..Furthermore, the immunologic mechanisms responsible for the antigen-specific unresponsiveness of peripheral blood B cells may be significant in the eventual progression from subclinical to clinical Johne's disease in cattle...
  23. doi request reprint Modulation of cytokine gene expression and secretion during the periparturient period in dairy cows naturally infected with Mycobacterium avium subsp. paratuberculosis
    E L Karcher
    Department of Animal Science, Iowa State University, Ames, IA 50010, USA
    Vet Immunol Immunopathol 123:277-88. 2008
    ..These data suggest that parturition is a very dynamic time period for host immunity, with potential for altered immunity to hinder the ability of dairy cows to thwart infectious diseases...
  24. ncbi request reprint Comparison of diagnostic detection methods for Mycobacterium avium subsp. paratuberculosis in North American bison
    J F J Huntley
    Department of Veterinary Pathology, Iowa State University, Ames, IA, USA
    Vet Pathol 42:42-51. 2005
    ..paratuberculosis was more sensitive than bacterial culture or staining, identified infection in all the bison, and detected the greatest number of positive tissues within each animal...
  25. ncbi request reprint Upregulation of transforming growth factor-beta and interleukin-10 in cows with clinical Johne's disease
    M S Khalifeh
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, 2300 Dayton Rd, Ames, IA 50010, USA
    Vet Immunol Immunopathol 99:39-46. 2004
    ..These results suggest that a change in the balance of cytokines at the site of infection may contribute to the ability of the host to control Mycobacterium avium subsp. paratuberculosis infection...
  26. ncbi request reprint Time delay, temperature effects and assessment of positive controls on whole blood for the gamma interferon ELISA to detect paratuberculosis
    S Robbe-Austerman
    National Animal Disease Center, Agricultural Research Service, USDA, 2300 Dayton Ames, IA 50010, USA
    J Vet Med B Infect Dis Vet Public Health 53:213-7. 2006
    ..The PPDs were more potent at stimulating IFN-gamma production than the whole cell sonicate. In conclusion, whole blood should be stored/transported at ambient room temperature and stimulated within 12 h of collection...
  27. doi request reprint Activation of immune cells in bovine mammary gland secretions by zymosan-treated bovine serum
    K Kimura
    USDA, Agricultural Research Service, National Animal Disease Center, Periparturient Diseases of Cattle Research Unit, Ames, IA 50010 0070, USA
    J Dairy Sci 91:1852-64. 2008
    ..The ZTS can stimulate PMN and lymphocyte-mediated immune defense mechanisms in the mammary gland, which may provide a useful means of preventing new intramammary infections during the dry period as well as at the initiation of lactation...
  28. ncbi request reprint Efficacy of immunologic assays for the detection of Johne's disease in dairy cows fed additional energy during the periparturient period
    Judith R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, Ames, IA 50010, USA
    J Vet Diagn Invest 16:412-20. 2004
    ..Furthermore, providing additional energy to cows with Johne's disease did not preclude immunosuppressive effects during the periparturient period...
  29. doi request reprint Osteopontin immunoreactivity in the ileum and ileocecal lymph node of dairy cows naturally infected with Mycobacterium avium subsp. paratuberculosis
    E L Karcher
    Department of Animal Science, Iowa State University, Ames, IA 50010, USA
    Vet Immunol Immunopathol 126:142-8. 2008
    ..The frequency of staining for Opn in the ICN was not affected by MAP infection. Results of this study confirm for the first time, the expression of Opn in the ileum and ICN of MAP-infected cattle...
  30. ncbi request reprint A simple, rapid, and effective method for the extraction of Mycobacterium paratuberculosis DNA from fecal samples for polymerase chain reaction
    J R Stabel
    USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    J Vet Diagn Invest 16:22-30. 2004
    ..The method described here is rapid, simple, and inexpensive compared with other techniques. In addition, this method can detect animals that are shedding less than 1 cfu/g...
  31. ncbi request reprint Cytokine gene expression in ileal tissues of cattle infected with Mycobacterium paratuberculosis
    H Lee
    Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, USDA-ARS, Ames, IA 50010, USA
    Vet Immunol Immunopathol 82:73-85. 2001
    ..paratuberculosis. These data demonstrate that localized ileal cytokine production is different between cows chronically infected with M. paratuberculosis and noninfected control cows...
  32. pmc Mediation of host immune responses after immunization of neonatal calves with a heat-killed Mycobacterium avium subsp. paratuberculosis vaccine
    J R Stabel
    USDA ARS, National Animal Disease Center, 1920 Dayton Ave, Building 20, Ames, IA 50010, USA
    Clin Vaccine Immunol 18:2079-89. 2011
    ..These results suggest that the Mycopar vaccine will interfere with diagnostic tools for paratuberculosis but result in low interference with the comparative cervical skin test and emerging serologic tests for M. bovis...
  33. doi request reprint Differential expression of CD5 on B lymphocytes in cattle infected with Mycobacterium avium subsp. paratuberculosis
    J R Stabel
    USDA ARS, National Animal Disease Center, Bacterial Diseases of Livestock Research Unit, 2300 Dayton Road, Ames, IA 50010, United States
    Vet Immunol Immunopathol 126:211-9. 2008
    ..These results suggest that differential expression of CD5bright and CD5dim subpopulations on B cells in animals with paratuberculosis may reflect a shift in host immunity during the disease process...
  34. ncbi request reprint Identification of a gene unique to Mycobacterium avium subspecies paratuberculosis and application to diagnosis of paratuberculosis
    J L Ellingson
    Zoonotics Disease Research Unit, National Animal Disease Center, USDA, Ames, Iowa 50010, USA
    Mol Cell Probes 12:133-42. 1998
    ..paratuberculosis. The dOH method could be a good alternative to existing assays and will be adapted for specific identification of M. paratuberculosis from faecal samples, mixed bacteriologic cultures, tissue specimens and whole blood...
  35. doi request reprint Pasteurization of colostrum reduces the incidence of paratuberculosis in neonatal dairy calves
    J R Stabel
    USDA ARS, National Animal Disease Center, 2300 Dayton Rd, Ames, IA 50010, USA
    J Dairy Sci 91:3600-6. 2008
    ..avium ssp. paratuberculosis, perhaps decreasing dissemination of infection over time...
  36. pmc Use of recombinant ESAT-6:CFP-10 fusion protein for differentiation of infections of cattle by Mycobacterium bovis and by M. avium subsp. avium and M. avium subsp. paratuberculosis
    W R Waters
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, Iowa 50010 0070
    Clin Diagn Lab Immunol 11:729-35. 2004
    ..avium subsp. paratuberculosis, it appears that use of the rESAT-6:CFP-10 fusion protein will be useful for the detection of tuberculous cattle in herds with pre-existing sensitization to M. avium and/or M. avium subsp. paratuberculosis...
  37. pmc Development and evaluation of an enzyme-linked immunosorbent assay for use in the detection of bovine tuberculosis in cattle
    W R Waters
    USDA, ARS, National Animal Disease Center, P O Box 70, Ames, IA 50010 0070, USA
    Clin Vaccine Immunol 18:1882-8. 2011
    ..bovis-infected dairy herds. A high-throughput ELISA could be adapted as a movement, border, or slaughter surveillance test, as well as a supplemental test to tuberculin skin testing...
  38. pmc Expression library immunization confers protection against Mycobacterium avium subsp. paratuberculosis infection
    J F Huntley
    College of Veterinary Medicine, Iowa State University, Ames, 50010, USA
    Infect Immun 73:6877-84. 2005
    ..This study is the first study to demonstrate protection against M. avium subsp. paratuberculosis infection with expression library immunization...
  39. ncbi request reprint Identification of two Mycobacterium avium subspecies paratuberculosis gene products differentially recognised by sera from rabbits immunised with live mycobacteria but not heat-killed mycobacteria
    J P Bannantine
    National Animal Disease Center, ARS USDA, Ames, IA 50010, USA
    J Med Microbiol 50:795-804. 2001
    ..These purified antibodies demonstrated that Csp1 was present within infected macrophages. Collectively, these data identify novel M. paratuberculosis antigens that may be important in pathogenesis...
  40. pmc Induction of B cell responses upon experimental infection of neonatal calves with Mycobacterium avium subsp. paratuberculosis
    J R Stabel
    USDA ARS, National Animal Disease Center, 1920 Dayton Ave, Building 20, Ames, IA 50010, USA
    Clin Vaccine Immunol 18:1139-49. 2011
    ..In summary, an induction of B cell responses was noted after experimental infection with M. avium subsp. paratuberculosis, with differences in responses noted according to the method of experimental inoculation...
  41. doi request reprint Parturition invokes changes in peripheral blood mononuclear cell populations in Holstein dairy cows naturally infected with Mycobacterium avium subsp. paratuberculosis
    E L Karcher
    Department of Animal Science, Iowa State University, Ames, IA 50010, United States
    Vet Immunol Immunopathol 124:50-62. 2008
    ..These results suggest changes in the percentages of lymphocyte subsets, monocytes, and CD5 markers are modulated by both infection status and the periparturient period...
  42. pmc Effects of gamma interferon, interleukin-10, and transforming growth factor beta on the survival of Mycobacterium avium subsp. paratuberculosis in monocyte-derived macrophages from naturally infected cattle
    M S Khalifeh
    National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, Iowa 50010, USA
    Infect Immun 72:1974-82. 2004
    ..avium subsp. paratuberculosis that may be directly related to their effects on macrophage activation and killing of M. avium subsp. paratuberculosis...
  43. ncbi request reprint Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR
    J L E Ellingson
    Zoonotics Disease Research Unit, National Animal Disease Center, USDA, Agriculture Research Service, P O Box 70, Ames, IA 50010, USA
    Mol Cell Probes 19:219-25. 2005
    ..Using these criteria, 25 of 25 bison tested were positive for MAP. The data indicate that these free-ranging bison have been infected by MAP...
  44. pmc Early immune markers associated with Mycobacterium avium subsp. paratuberculosis infection in a neonatal calf model
    J R Stabel
    USDA ARS, National Animal Disease Center, 1920 Dayton Ave, Building 20, Ames, IA 50010, USA
    Clin Vaccine Immunol 18:393-405. 2011
    ..These were followed by antigen-specific lymphocyte proliferation, iNOS secretion, and expression of CD26 and CD5(bright) markers in the latter part of the 12-month study...
  45. doi request reprint Characteristics of an extensive Mycobacterium avium subspecies paratuberculosis recombinant protein set
    John P Bannantine
    National Animal Disease Center, USDA ARS, Ames, IA 50010, United States
    Protein Expr Purif 72:223-33. 2010
    ..This extensive recombinant protein repository provides a powerful tool for proteome- and genome-scale research of this organism...
  46. doi request reprint Development and use of a partial Mycobacterium avium subspecies paratuberculosis protein array
    John P Bannantine
    National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    Proteomics 8:463-74. 2008
    ..paratuberculosis surface proteins for immune profiling experiments in cattle...
  47. pmc Profiling bovine antibody responses to Mycobacterium avium subsp. paratuberculosis infection by using protein arrays
    John P Bannantine
    National Animal Disease Center, ARS USDA, 2300 North Dayton Avenue, Ames, IA 50010, USA
    Infect Immun 76:739-49. 2008
    ..This powerful combination of genomic information, molecular tools, and immunological assays has enabled the identification of previously unknown antigens of M. avium subsp. paratuberculosis...
  48. ncbi request reprint Experimental infection of white-tailed deer (Odocoileus virginianus) with Mycobacterium avium subsp. paratuberculosis
    Mitchell V Palmer
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2300 Dayton Avenue, Ames, Iowa 50010, USA
    J Wildl Dis 43:597-608. 2007
    ..These findings suggest that experimental oral inoculation of white-tailed deer fawns may mimic all aspects of subclinical and clinical paratuberculosis...
  49. pmc Expression and immunogenicity of proteins encoded by sequences specific to Mycobacterium avium subsp. paratuberculosis
    John P Bannantine
    National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 50010, USA
    J Clin Microbiol 42:106-14. 2004
    ..avium subsp. paratuberculosis antigens that are not present in any other mycobacteria. These findings may have a major impact on improved diagnostics for Johne's disease...
  50. ncbi request reprint Application of the genome sequence to address concerns that Mycobacterium avium subspecies paratuberculosis might be a foodborne pathogen
    John P Bannantine
    USDA ARS National Animal Disease Center, Ames, Iowa 50010, USA
    Foodborne Pathog Dis 1:3-15. 2004
    ..Application of these new tools in addressing foodborne related issues of M. paratuberculosis is discussed...
  51. ncbi request reprint Isolation of Mycobacterium avium subsp paratuberculosis (Map) from feral cats on a dairy farm with Map-infected cattle
    Mitchell V Palmer
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010, USA
    J Wildl Dis 41:629-35. 2005
    ..Shedding of Map from infected cats was not evaluated. The epidemiologic role of Map-infected feral cats on dairy farms requires further investigation...
  52. ncbi request reprint Evaluation of the gamma interferon ELISA in sheep subclinically infected with Mycobacterium avium subspecies paratuberculosis using a whole-cell sonicate or a johnin purified-protein derivative
    Suelee Robbe-Austerman
    Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA 50010, USA
    J Vet Diagn Invest 18:189-94. 2006
    ..25 for sensitivity of 66.7% (47.2-82.7) and specificity of 93.5% (85.5-97.9) or 98.3% (96.4-99.4), respectively. The PPDj was more accurate at identifying MAP-infected sheep than was the MpS (P = 0.034)...
  53. pmc Proteome and differential expression analysis of membrane and cytosolic proteins from Mycobacterium avium subsp. paratuberculosis strains K-10 and 187
    Thomas J Radosevich
    USDA ARS, National Animal Disease Center, 2300 North Dayton Avenue, Ames, IA 50010, USA
    J Bacteriol 189:1109-17. 2007
    ..Results from these studies will provide tools for developing a better understanding of M. avium subsp. paratuberculosis infection in the host and offer potential as diagnostic reagents and vaccine candidates...
  54. pmc Production and characterization of monoclonal antibodies against a major membrane protein of Mycobacterium avium subsp. paratuberculosis
    John P Bannantine
    National Animal Disease Center, ARS USDA, 2300 North Dayton Ave, Ames, IA 50010, USA
    Clin Vaccine Immunol 14:312-7. 2007
    ..In this study, purified recombinant MMP was used to produce two stable monoclonal antibodies, termed 8G2 and 13E1, which were characterized by immunoblotting, epitope mapping, and immunofluorescence microscopy...
  55. pmc Development and characterization of monoclonal antibodies and aptamers against major antigens of Mycobacterium avium subsp. paratuberculosis
    John P Bannantine
    National Animal Disease Center, ARS USDA, 2300 North Dayton Avenue, Ames, IA 50010, USA
    Clin Vaccine Immunol 14:518-26. 2007
    ..avium subsp. paratuberculosis as well as other mycobacteria. These detection reagents may be beneficial in many JD research applications...
  56. ncbi request reprint Skin test and gamma interferon enzyme-linked immunosorbent assay results in sheep exposed to dead Mycobacterium avium subspecies paratuberculosis organisms
    Suelee Robbe-Austerman
    VDPAM ISU, 1717 Vet Med, Ames, IA 50011, USA
    J Vet Diagn Invest 19:88-90. 2007
    ..These results suggest that infection/invasion of MAP organisms must occur in order to have a positive skin test or IFN-gamma ELISA beyond the false-positive rate. Simple exposure is not enough to elicit a detectable CMI response...
  57. ncbi request reprint The Mycobacterium avium subsp. paratuberculosis 35 kDa protein plays a role in invasion of bovine epithelial cells
    John P Bannantine
    National Animal Disease Center, USDA ARS, 2300 North Dayton Avenue, Ames, IA 50010, USA
    Microbiology 149:2061-9. 2003
    ..The authors suggest that the MMP is a virulence factor of M. paratuberculosis that may be important in the initiation of infection in vivo...
  58. ncbi request reprint Expression of adhesion molecules on milk and blood lymphocytes from periparturient dairy cattle with Johne's disease
    James A Harp
    Periparturient Diseases of Cattle Research Unit, Ames, IA 50010, USA
    Vet Immunol Immunopathol 98:69-76. 2004
    ..Increased percentages of T lymphocytes expressing adhesion molecules in milk compared to blood suggest that a migratory population of cells is being selectively recruited to the mammary gland from the circulation...
  59. pmc Disparate host immunity to Mycobacterium avium subsp. paratuberculosis antigens in calves inoculated with M. avium subsp. paratuberculosis, M. avium subsp. avium, M. kansasii, and M. bovis
    J R Stabel
    USDA ARS, National Animal Disease Center, Ames, IA, USA
    Clin Vaccine Immunol 20:848-57. 2013
    ..paratuberculosis and those inoculated with M. avium that were somewhat disparate from the responses in calves infected with M. bovis, suggesting that the biology of mycobacterial infection plays an important role in diagnosis...
  60. pmc Chemical decontamination with N-acetyl-L-cysteine-sodium hydroxide improves recovery of viable Mycobacterium avium subsp. paratuberculosis organisms from cultured milk
    L Bradner
    Roy J Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa, USA
    J Clin Microbiol 51:2139-46. 2013
    ..Therefore, it is important to optimize milk decontamination protocols to ensure that low concentrations of M. avium subsp. paratuberculosis can be detected...
  61. doi request reprint Evaluation of protection in a mouse model after vaccination with Mycobacterium avium subsp. paratuberculois protein cocktails
    J R Stabel
    USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    Vaccine 31:127-34. 2012
    ..The present study demonstrated that MAP proteins are useful as vaccine candidates to reduce MAP tissue burden...
  62. pmc Optimization of hexadecylpyridinium chloride decontamination for culture of Mycobacterium avium subsp. paratuberculosis from milk
    L Bradner
    Roy J Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa, USA
    J Clin Microbiol 51:1575-7. 2013
    ..This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the culturing protocol, and such protocols should be optimized to ensure that low concentrations of MAP in milk can be detected...
  63. ncbi request reprint Short communication: Allele, genotype, and haplotype data for bovine spongiform encephalopathy-resistance polymorphisms from healthy US Holstein cattle
    B W Brunelle
    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA
    J Dairy Sci 91:338-42. 2008
    ..Based on the frequencies of these regulatory region polymorphisms, the US Holstein population is not at a greater risk for BSE than Holsteins worldwide...
  64. ncbi request reprint Experimental challenge models for Johne's disease: a review and proposed international guidelines
    Murray E Hines
    Tifton Veterinary Diagnostic and Investigational Laboratory, University of Georgia, Tifton, GA 31793, USA
    Vet Microbiol 122:197-222. 2007
    ..paratuberculosis used, challenge dose, dose frequency, age of challenge, route of challenge, preparation of inoculum, experimental animal selection, quality control, minimal experimental endpoints and other parameters...
  65. pmc Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis
    Alifiya S Motiwala
    Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, 44691, USA
    J Clin Microbiol 41:2015-26. 2003
    ..Further, the results of our analyses reveal a relatively higher degree of genetic heterogeneity among M. avium subsp. paratuberculosis isolates recovered from human and ovine sources...
  66. pmc Application of the C(18)-carboxypropylbetaine specimen processing method to recovery of Mycobacterium avium subsp. paratuberculosis from ruminant tissue specimens
    Charles G Thornton
    Integrated Research Technology, LLC, c o Quest Diagnostics Incorporated, 1901 Sulfur Spring Road, Baltimore, MD 21227, USA
    J Clin Microbiol 40:1783-90. 2002
    ..avium subsp. paratuberculosis from 26 and 16 specimens, respectively. The average times to positive were 7.4 +/- 8.3, 29.9 +/- 2.6, and 24 +/- 0 days, respectively. The contamination rates were 4.8, 22.6, and 20.0%, respectively...
  67. ncbi request reprint Johne's disease in cattle is associated with enhanced expression of genes encoding IL-5, GATA-3, tissue inhibitors of matrix metalloproteinases 1 and 2, and factors promoting apoptosis in peripheral blood mononuclear cells
    Paul M Coussens
    Center for Animal Functional Genomics, Department of Animal Science, Michigan State University, 1205H Anthony Hall, East Lansing, MI 4882, USA
    Vet Immunol Immunopathol 105:221-34. 2005
    ..paratuberculosis-infected cows are likely poised for rapid apoptosis (upregulation of CIDE-A, Bad, TNFRI, and Fas)...