G R Siragusa

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. pmc Real-time monitoring of Escherichia coli O157:H7 adherence to beef carcass surface tissues with a bioluminescent reporter
    G R Siragusa
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska 68933 0166, USA
    Appl Environ Microbiol 65:1738-45. 1999
  2. ncbi request reprint The incidence of Escherichia coli on beef carcasses and its association with aerobic mesophilic plate count categories during the slaughter process
    G R Siragusa
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska 68933, USA
    J Food Prot 61:1269-74. 1998
  3. ncbi request reprint Development of a multiple-step process for the microbial decontamination of beef trim
    D H Kang
    U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA
    J Food Prot 64:63-71. 2001
  4. ncbi request reprint Long-term effect of alkaline, organic acid, or hot water washes on the microbial profile of refrigerated beef contaminated with bacterial pathogens after washing
    W J Dorsa
    United States Department of Agriculture, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska68933 0166, USA
    J Food Prot 61:300-6. 1998
  5. ncbi request reprint Bacterial cross-contamination of meat during liquid nitrogen immersion freezing
    E D Berry
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska 68933 0166, USA
    J Food Prot 61:1103-8. 1998
  6. ncbi request reprint Incorporation of nisin into a meat binding system to inhibit bacteria on beef surfaces
    C N Cutter
    United States Department of Agriculture, Roman L Hruska U S Meat Animal Research Center, Clay Center, NE, USA
    Lett Appl Microbiol 27:19-23. 1998
  7. pmc Genotypic analyses of Escherichia coli O157:H7 and O157 nonmotile isolates recovered from beef cattle and carcasses at processing plants in the Midwestern states of the United States
    G A Barkocy-Gallagher
    Roman L Hruska U S Meat Animal Research Center, Agricultural Research Service, United States Department of Agriculture, Clay Center, Nebraska 68933 0166, USA
    Appl Environ Microbiol 67:3810-8. 2001
  8. ncbi request reprint Evaluation of combination treatment processes for the microbial decontamination of pork trim
    M M Castelo
    U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA
    J Food Prot 64:335-42. 2001
  9. ncbi request reprint Monitoring the microbial contamination of beef carcass tissue with a rapid chromogenic Limulus amoebocyte lysate endpoint assay
    G R Siragusa
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, NE, USA
    Lett Appl Microbiol 31:178-83. 2000
  10. doi request reprint Clostridium difficile from healthy food animals: optimized isolation and prevalence
    S N Thitaram
    Bacterial Epidemiology and Antimicrobial Resistance Research Unit, U S Department of Agriculture, Agricultural Research Service, 950 College Station Road, Athens, Georgia 30605, USA
    J Food Prot 74:130-3. 2011

Detail Information

Publications24

  1. pmc Real-time monitoring of Escherichia coli O157:H7 adherence to beef carcass surface tissues with a bioluminescent reporter
    G R Siragusa
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska 68933 0166, USA
    Appl Environ Microbiol 65:1738-45. 1999
    ..98). Real-time assessment of microbial attachment to this complex menstruum should facilitate evaluation of carcass decontamination procedures and mechanistic studies of microbial contamination of beef carcass tissues...
  2. ncbi request reprint The incidence of Escherichia coli on beef carcasses and its association with aerobic mesophilic plate count categories during the slaughter process
    G R Siragusa
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska 68933, USA
    J Food Prot 61:1269-74. 1998
    ..coli-positive sample. Using multivariate analysis to account for influences of plant and within plant processing site, the data indicated a strong positive linear trend between the presence of E. coli and the APC class...
  3. ncbi request reprint Development of a multiple-step process for the microbial decontamination of beef trim
    D H Kang
    U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA
    J Food Prot 64:63-71. 2001
    ..Based on microbial reduction and quality aspects, it was concluded that successively applied combination antimicrobial treatments for meat trim could offer potential food safety benefits...
  4. ncbi request reprint Long-term effect of alkaline, organic acid, or hot water washes on the microbial profile of refrigerated beef contaminated with bacterial pathogens after washing
    W J Dorsa
    United States Department of Agriculture, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska68933 0166, USA
    J Food Prot 61:300-6. 1998
    ....
  5. ncbi request reprint Bacterial cross-contamination of meat during liquid nitrogen immersion freezing
    E D Berry
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, Nebraska 68933 0166, USA
    J Food Prot 61:1103-8. 1998
    ..Results demonstrate that bacterial cross-contamination of meat during LN immersion freezing can occur but indicate that the use of good sanitation practices and product with low microbial numbers can limit this occurrence...
  6. ncbi request reprint Incorporation of nisin into a meat binding system to inhibit bacteria on beef surfaces
    C N Cutter
    United States Department of Agriculture, Roman L Hruska U S Meat Animal Research Center, Clay Center, NE, USA
    Lett Appl Microbiol 27:19-23. 1998
    ..These studies indicate that the addition of a bacteriocin to a meat binding system and application to meat surfaces may be useful in reducing undesirable bacteria in restructured meat products...
  7. pmc Genotypic analyses of Escherichia coli O157:H7 and O157 nonmotile isolates recovered from beef cattle and carcasses at processing plants in the Midwestern states of the United States
    G A Barkocy-Gallagher
    Roman L Hruska U S Meat Animal Research Center, Agricultural Research Service, United States Department of Agriculture, Clay Center, Nebraska 68933 0166, USA
    Appl Environ Microbiol 67:3810-8. 2001
    ..coli O157 carcass contamination originates from animals within the same lot and not from cross-contamination between lots. In addition, the data demonstrate that most carcass contamination occurs very early during processing...
  8. ncbi request reprint Evaluation of combination treatment processes for the microbial decontamination of pork trim
    M M Castelo
    U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA
    J Food Prot 64:335-42. 2001
    ..Water and water plus lactic acid were the most favorable treatments in reducing microbial populations on pork trim without affecting the quality attributes of the ground pork...
  9. ncbi request reprint Monitoring the microbial contamination of beef carcass tissue with a rapid chromogenic Limulus amoebocyte lysate endpoint assay
    G R Siragusa
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, Clay Center, NE, USA
    Lett Appl Microbiol 31:178-83. 2000
    ..For beef carcass testing, this assay can be used with no instrumentation other than the required 37 degrees C incubator and, as an option, a microplate reader...
  10. doi request reprint Clostridium difficile from healthy food animals: optimized isolation and prevalence
    S N Thitaram
    Bacterial Epidemiology and Antimicrobial Resistance Research Unit, U S Department of Agriculture, Agricultural Research Service, 950 College Station Road, Athens, Georgia 30605, USA
    J Food Prot 74:130-3. 2011
    ..difficile from dairy cattle feces. This study suggests that food animals might harbor C. difficile and it provides critical information that isolation methods might not have universal application across animal species...
  11. ncbi request reprint Reduction of Brochothrix thermosphacta on beef surfaces following immobilization of nisin in calcium alginate gels
    C N Cutter
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska US Meat Animal Research Center, Clay Center, NE 68933, USA
    Lett Appl Microbiol 23:9-12. 1996
    ..This study demonstrates that immobilization of nisin in a gel may be a more effective delivery system of a bacteriocin to the carcass surface than direct application...
  12. ncbi request reprint Screening bovine carcass sponge samples for Escherichia coli O157 using a short enrichment coupled with immunomagnetic separation and a polymerase chain reaction-based (BAX) detection stept
    D H Kang
    US Department of Agriculture, Agricultural Research Service, Roman L. Hruska US Meat Animal Research Center, Nebraska 68933, USA
    J Food Prot 64:1610-2. 2001
    ..coli O157:H7 in carcass sponge samples. Our data indicate that optimization and subsequent testing of this protocol for use as a carcass screening tool are warranted...
  13. ncbi request reprint A rapid twofold dilution method for microbial enumeration and resuscitation of uninjured and sublethally injured bacteria
    D H Kang
    United States Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE 68933-0166, USA
    Lett Appl Microbiol 33:232-6. 2001
    ..SIGNIFICANCE AND IMPACT OF THE STUDY: The twofold dilution method recovered significantly higher numbers of heat-injured coliforms compared to conventional plating methods (P < 0.05)...
  14. doi request reprint Antimicrobial activity of lupulone against Clostridium perfringens in the chicken intestinal tract jejunum and caecum
    G R Siragusa
    Agricultural Research Service, United States Department of Agriculture, Poultry Microbiological Safety Research UnitRussell Research Center, Athens, GA, USA
    J Antimicrob Chemother 61:853-8. 2008
    ..Hop (Humulus lupulus) bitter acids are known to possess potent antimicrobial activity. Lupulone was evaluated for in vivo antimicrobial activity to inhibit C. perfringens in a chick gastrointestinal colonization model...
  15. ncbi request reprint Campylobacter spp. subtype analysis using gel-based repetitive extragenic palindromic-PCR discriminates in parallel fashion to flaA short variable region DNA sequence analysis
    K L Hiett
    U S Department of Agriculture, Agricultural Research Service, Poultry Microbiological Safety Research Unit, Russell Research Center, Athens, GA 30604 5677, USA
    J Appl Microbiol 101:1249-58. 2006
    ..These data were then used for comparison with the established genotyping method of flaA short variable region (SVR) DNA sequence analysis as a tool for molecular epidemiology...
  16. ncbi request reprint Quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets
    M G Wise
    Poultry Microbiological Safety Research Unit, Russell Research Center, Agricultural Research Service, United States Department of Agriculture, Athens, GA 30605, USA
    J Appl Microbiol 102:1138-49. 2007
    ....
  17. ncbi request reprint Improved antimicrobial activity of nisin-incorporated polymer films by formulation change and addition of food grade chelator
    C N Cutter
    United States Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, PO Box 166, Clay Center, NE 68933, USA
    Lett Appl Microbiol 33:325-8. 2001
    ..The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin)...
  18. ncbi request reprint Stationary-phase acid resistance and injury of recent bovine Escherichia coli O157 and non-O157 biotype I Escherichia coli isolatest
    E D Berry
    U S Department of Agriculture, Agricultural Research Service, Roman L Hruska U S Meat Animal Research Center, P O Box 166, Spur 18D, Clay Center, Nebraska 68933 0166, USA
    J Food Prot 67:583-90. 2004
    ..coli O157 further supports the idea that the previous environment of this pathogen should be a consideration when designing microbial safety strategies for foods preserved by low pH and acid...
  19. ncbi request reprint Bifidobacterium-selective isolation and enumeration from chicken caeca by a modified oligosaccharide antibiotic-selective agar medium
    S N Thitaram
    Agricultural Research Service, United States Department of Agriculture, Russell Research Center, Athens, GA 30605, USA
    Lett Appl Microbiol 41:355-60. 2005
    ..To determine the efficacy and selectivity of an acidified, antibiotic-selective, oligosaccharide-containing media for enumerating Bifidobacterium spp. from chicken caeca samples...
  20. doi request reprint Effects of direct-fed microbials on growth performance, gut morphometry, and immune characteristics in broiler chickens
    K W Lee
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, USDA, Beltsville, MD 20705, USA
    Poult Sci 89:203-16. 2010
    ..These results provide a rational scientific basis for future studies to investigate DFM as immunomodulating agents to enhance host protective immunity against enteric pathogens in broiler chickens...
  21. pmc Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR
    G R Siragusa
    Agricultural Research Service, United States Department of Agriculture, Russell Research Center, Athens, Georgia 30604 5677, USA
    J Clin Microbiol 44:1065-73. 2006
    ..Repetitive sequence-based PCR was found to be a technically practical and reproducible means of subtyping C. perfringens libraries from specific epidemiological or production environment settings...
  22. pmc Isolation and purification of enterocin E-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria
    J E Line
    USDA ARS, Russell Research Center, Poultry Microbiological Safety Research Unit, 950 College Station Rd, Athens, GA 30604, USA
    Antimicrob Agents Chemother 52:1094-100. 2008
    ..Enterocin E-760 also significantly (P < 0.05) reduced the colonization of naturally acquired Campylobacter species in market age broiler chickens when administered in treated feed 4 days prior to analysis...
  23. ncbi request reprint Presence of naturally occurring Campylobacter and Salmonella in the mature and immature ovarian follicles of late-life broiler breeder hens
    N A Cox
    USDA ARS, PMSRU, Russell Research Center, 950 College Station Road, Athens, GA 30605, USA
    Avian Dis 49:285-7. 2005
    ....
  24. ncbi request reprint Isomaltooligosaccharide increases cecal Bifidobacterium population in young broiler chickens
    S N Thitaram
    Department of Food Science and Technology, University of Georgia, Athens, Georgia 30602, USA
    Poult Sci 84:998-1003. 2005
    ....