Timothy A Reinhardt

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. doi request reprint The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1)
    Timothy A Reinhardt
    Ruminant Diseases and Immunology Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010, USA Electronic address
    Biochem Biophys Res Commun 445:417-21. 2014
  2. doi request reprint Bovine milk proteome: quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis
    Timothy A Reinhardt
    Ruminant Diseases and Immunology Unit, National Animal Disease Center, USDA ARS, Ames IA 50010, USA
    J Proteomics 82:141-54. 2013
  3. doi request reprint Bovine milk exosome proteome
    Timothy A Reinhardt
    Ruminant Diseases and Immunology Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    J Proteomics 75:1486-92. 2012
  4. doi request reprint Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk
    T A Reinhardt
    Periparturient Diseases of Cattle Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA
    J Dairy Sci 91:2307-18. 2008
  5. doi request reprint Mammary gland involution is associated with rapid down regulation of major mammary Ca2+-ATPases
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, Agricultural Research Service USDA, National Animal Disease Center, 2300 Dayton Ave, Ames, IA 50010, USA
    Biochem Biophys Res Commun 378:99-102. 2009
  6. doi request reprint Prevalence of subclinical hypocalcemia in dairy herds
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA
    Vet J 188:122-4. 2011
  7. pmc Neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants
    Randy E Sacco
    Ruminant Diseases and Immunology Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA
    Viruses 4:3731-53. 2012
  8. ncbi request reprint Bovine milk fat globule membrane proteome
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010 USA
    J Dairy Res 73:406-16. 2006
  9. doi request reprint Proteomic changes in Escherichia coli when grown in fresh milk versus laboratory media
    John D Lippolis
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, Iowa 50010, USA
    J Proteome Res 8:149-58. 2009
  10. pmc Differential chemokine and cytokine production by neonatal bovine γδ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection
    Jodi L McGill
    Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, Ames, IA 50010, USA
    Immunology 139:227-44. 2013

Detail Information

Publications38

  1. doi request reprint The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca(2+) ATPase (SPCA1)
    Timothy A Reinhardt
    Ruminant Diseases and Immunology Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010, USA Electronic address
    Biochem Biophys Res Commun 445:417-21. 2014
    ....
  2. doi request reprint Bovine milk proteome: quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis
    Timothy A Reinhardt
    Ruminant Diseases and Immunology Unit, National Animal Disease Center, USDA ARS, Ames IA 50010, USA
    J Proteomics 82:141-54. 2013
    ..Protein composition changes found in milk exosomes, MFGM and whey during an infection provides new and comprehensive information on milk protein composition in general as well as changes occurring during an infection...
  3. doi request reprint Bovine milk exosome proteome
    Timothy A Reinhardt
    Ruminant Diseases and Immunology Unit, National Animal Disease Center, USDA ARS, Ames, IA 50010, USA
    J Proteomics 75:1486-92. 2012
    ..The protein composition of milk exosomes provides new information on milk protein composition and the potential physiological significance of exosomes to mammary physiology...
  4. doi request reprint Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk
    T A Reinhardt
    Periparturient Diseases of Cattle Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA
    J Dairy Sci 91:2307-18. 2008
    ..6- to 4.3-fold in d 7 MFGM compared with colostrum MFGM. These data demonstrate that quantitative shotgun proteomics has great potential to provide new insights into mammary development...
  5. doi request reprint Mammary gland involution is associated with rapid down regulation of major mammary Ca2+-ATPases
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, Agricultural Research Service USDA, National Animal Disease Center, 2300 Dayton Ave, Ames, IA 50010, USA
    Biochem Biophys Res Commun 378:99-102. 2009
    ....
  6. doi request reprint Prevalence of subclinical hypocalcemia in dairy herds
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA
    Vet J 188:122-4. 2011
    ..Subclinical hypocalcemia may make cows more susceptible to secondary diseases but more research will be required to determine if this is true...
  7. pmc Neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants
    Randy E Sacco
    Ruminant Diseases and Immunology Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA
    Viruses 4:3731-53. 2012
    ..This review will summarize available data that provide parallels between bovine RSV infection in neonatal calves and human RSV in infants...
  8. ncbi request reprint Bovine milk fat globule membrane proteome
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010 USA
    J Dairy Res 73:406-16. 2006
    ..The finding of CD14, toll like receptor (TLR2), and TLR4 on MFGM suggests a direct role for the mammary gland in detecting an infection...
  9. doi request reprint Proteomic changes in Escherichia coli when grown in fresh milk versus laboratory media
    John D Lippolis
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, Iowa 50010, USA
    J Proteome Res 8:149-58. 2009
    ..Growth of a mammary pathogenic E. coli in milk results in the up- and down-regulation of hundreds of proteins, some of which may be important to the pathogenesis of this organism...
  10. pmc Differential chemokine and cytokine production by neonatal bovine γδ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection
    Jodi L McGill
    Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, Ames, IA 50010, USA
    Immunology 139:227-44. 2013
    ..Based on our results, we propose that circulating γδ T cells are poised to rapidly respond to viral infection and suggest an important role for γδ T cells in the innate immune response of the bovine neonate...
  11. pmc Treatment of an intramammary bacterial infection with 25-hydroxyvitamin D(3)
    John D Lippolis
    Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, United States of America
    PLoS ONE 6:e25479. 2011
    ....
  12. ncbi request reprint Null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2 impairs calcium transport into milk
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, United States Department of Agriculture Agriculture Research Service, National Animal Disease Center, Ames, Iowa 50010, USA
    J Biol Chem 279:42369-73. 2004
    ..This major secretory function represents a novel biological role for the plasma membrane Ca2+-ATPases, which are generally regarded as premier regulators of intracellular Ca2+...
  13. pmc Differential expression of cytokines in response to respiratory syncytial virus infection of calves with high or low circulating 25-hydroxyvitamin D3
    Randy E Sacco
    Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, United States Department of Agriculture, Agricultural Research Service, Ames, Iowa, United States of America
    PLoS ONE 7:e33074. 2012
    ..Further examination of the potential effects of vitamin D status on RSV disease resolution will require longer-term studies with immunologically sufficient and deficient vitamin D levels...
  14. ncbi request reprint Adapting to the transition between gestation and lactation: differences between rat, human and dairy cow
    Ronald L Horst
    National Animal Disease Center, United States Department of Agriculture, Ames, Iowa, 50010, USA
    J Mammary Gland Biol Neoplasia 10:141-56. 2005
    ....
  15. ncbi request reprint Neutrophil extracellular trap formation by bovine neutrophils is not inhibited by milk
    John D Lippolis
    Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, Ames, IA 50010, USA
    Vet Immunol Immunopathol 113:248-55. 2006
    ..Therefore, being unaffected by incubation in milk may indicate an important role for neutrophil extracellular traps in defense against mastitis...
  16. pmc Regulation of Mycobacterium-specific mononuclear cell responses by 25-hydroxyvitamin D3
    Corwin D Nelson
    Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, United States of America
    PLoS ONE 6:e21674. 2011
    ....
  17. ncbi request reprint Short communication: Ca2+-adenosine triphosphatase protein expression in the mammary gland of periparturient cows
    S Prapong
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    J Dairy Sci 88:1741-4. 2005
    ..The secretory pathway Ca2+-ATPase was elevated prepartum in mammary tissue from cows that developed MF compared with non-MF cows...
  18. pmc Proteome and differential expression analysis of membrane and cytosolic proteins from Mycobacterium avium subsp. paratuberculosis strains K-10 and 187
    Thomas J Radosevich
    USDA ARS, National Animal Disease Center, 2300 North Dayton Avenue, Ames, IA 50010, USA
    J Bacteriol 189:1109-17. 2007
    ..Results from these studies will provide tools for developing a better understanding of M. avium subsp. paratuberculosis infection in the host and offer potential as diagnostic reagents and vaccine candidates...
  19. ncbi request reprint Role of vitamin D in calcium homeostasis and its use in prevention of bovine periparturient paresis
    R L Horst
    United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Periparturient Diseases of Cattle Research Unit, Ames, Iowa 50010, USA
    Acta Vet Scand Suppl 97:35-50. 2003
    ..Vitamin D sterols have played a significant role in efforts to avoid parturient hypocalcemia and this report will summarize advantages and disadvantages associated with their use...
  20. doi request reprint Serum 25-hydroxyvitamin D concentrations in captive and free-ranging, white-tailed deer (Odocoileus virginianus)
    W Ray Waters
    National Animal Disease Center, Ames, Iowa 50010, USA
    Int J Vitam Nutr Res 79:180-7. 2009
    ..Additional research is necessary to determine if low serum 25(OH)D concentrations during the winter or pre-weaning period are associated with increased rates of infectious and metabolic disease...
  21. pmc In vivo activation of the intracrine vitamin D pathway in innate immune cells and mammary tissue during a bacterial infection
    Corwin D Nelson
    Diseases and Immunology Research Unit, National Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Ames, Iowa, USA
    PLoS ONE 5:e15469. 2010
    ....
  22. ncbi request reprint Differential expression analysis of proteins from neutrophils in the periparturient period and neutrophils from dexamethasone-treated dairy cows
    John D Lippolis
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    Vet Immunol Immunopathol 111:149-64. 2006
    ....
  23. ncbi request reprint Proteomic survey of bovine neutrophils
    John D Lippolis
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    Vet Immunol Immunopathol 103:53-65. 2005
    ..Proteins important for cell signaling, vesicular transport, control of apoptosis and other functions were identified giving an overview of the bovine neutrophil proteome...
  24. ncbi request reprint Ca(2+)-ATPase protein expression in mammary tissue
    T A Reinhardt
    Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA
    Am J Physiol Cell Physiol 279:C1595-602. 2000
    ..The abundance and cell location of PMCA2b suggest that it is important for macro-Ca(2+) homeostasis in lactating tissue. The pattern of expression and abundance of SPCA suggest that it is a candidate for the Golgi Ca(2+)-ATPase...
  25. doi request reprint Short communication: the preruminant calf as a model for characterizing the effects of vitamin D status in the neonate
    B J Nonnecke
    Periparturient Diseases of Cattle Research Unit, USDA ARS National Animal Disease Center, Ames, IA 50010 0070, USA
    J Dairy Sci 92:5692-6. 2009
    ....
  26. ncbi request reprint 1,25-Dihydroxyvitamin D(3) and 9-cis-retinoids are synergistic regulators of 24-hydroxylase activity in the rat and 1, 25-dihydroxyvitamin D(3) alters retinoic acid metabolism in vivo
    T A Reinhardt
    Agricultural Research Service, United States Department of Agriculture, Ames, Iowa, 50010, USA
    Arch Biochem Biophys 368:244-8. 1999
    ..These results show that 9-cis-RA can act synergistically with 1,25(OH)(2)D(3) in the regulation of 24-hydroxylase in vivo. Additionally, 1,25(OH)(2)D(3) regulates 9, 13-di-cis-RA metabolism in vivo...
  27. ncbi request reprint Ca2+-ATPases and their expression in the mammary gland of pregnant and lactating rats
    T A Reinhardt
    Metabolic Diseases and Immunology Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA
    Am J Physiol 276:C796-802. 1999
    ..The pattern of expression and abundance of RS-10 suggest that it is a candidate for the Golgi Ca2+-ATPase shown to be important in maintaining the Golgi Ca2+ concentration required for casein synthesis and micelle formation...
  28. ncbi request reprint Parturition and hypocalcemia blunts calcium signals in immune cells of dairy cattle
    K Kimura
    USDA, Agricultural Research Service, National Animal Disease Center, Periparturient Diseases of Cattle Research Unit, Ames, IA 50010 0070, USA
    J Dairy Sci 89:2588-95. 2006
    ..This decrease in PBMC intracellular Ca stores before parturition and the development of hypocalcemia contributes to periparturient immune suppression...
  29. ncbi request reprint Calcium and vitamin D metabolism during lactation
    R L Horst
    U S Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Metabolic Diseases and Immunology Research Unit, Ames, Iowa 50010 0070, USA
    J Mammary Gland Biol Neoplasia 2:253-63. 1997
    ..Finally, we will review what is known about the regulation of calcium transfer from the plasma to the milk...
  30. ncbi request reprint Characterization of Cos-7 cells overexpressing the rat secretory pathway Ca2+-ATPase
    Timothy A Reinhardt
    Periparturient Diseases of Cattle Research Unit, U S Department of Agriculture Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA
    Am J Physiol Cell Physiol 286:C164-9. 2004
    ..These data demonstrate that overexpression of the rat SPCA results in significant changes in the expression of calcium transport and storage proteins in COS-7 cells...
  31. ncbi request reprint Characterization of a factor from bovine intestine that protects against Cryptosporidium parvum infection
    Dhuha Akili
    Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, IA 50010, United States
    Vet Parasitol 142:168-72. 2006
    ..parvum at 9 days were significantly less infected than controls upon necropsy at 15 days of age. These data suggest that a protein with N-terminal sequence homology to LAP may reduce susceptibility of infant rats to C. parvum infection...
  32. ncbi request reprint Calcitroic acid is a major catabolic metabolite in the metabolism of 1 alpha-dihydroxyvitamin D(2)
    D R Zimmerman
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Arch Biochem Biophys 392:14-22. 2001
    ..Intermediates leading to the formation of the calcitroic acid in the 1 alpha,25-dihydroxyvitamin D(2) metabolism pathway are currently being studied...
  33. ncbi request reprint Decreased neutrophil function as a cause of retained placenta in dairy cattle
    Kayoko Kimura
    USDA, Agricultural Research Service, National Animal Disease Center, Periparturient Disease of Cattle Research Unit, Ames, IA 50010 0070, USA
    J Dairy Sci 85:544-50. 2002
    ..From these data, we suggest that neutrophil function is a determining factor for the development of RP in dairy cattle. Also, depressed production of IL-8 may be a factor affecting neutrophil function in cows developing RP...
  34. doi request reprint Utility, limitations, and promise of proteomics in animal science
    John D Lippolis
    Ruminant Diseases and Immunology Research Unit, USDA ARS, National Animal Disease Center, 1920 Dayton Avenue, Ames, IA 50010, United States
    Vet Immunol Immunopathol 138:241-51. 2010
    ..In addition, challenges and limitations of proteomics are presented, stressing those that are unique to veterinary/animal sciences...
  35. doi request reprint Modulation of the bovine innate immune response by production of 1alpha,25-dihydroxyvitamin D(3) in bovine monocytes
    C D Nelson
    Periparturient Diseases of Cattle Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, IA 50010, USA
    J Dairy Sci 93:1041-9. 2010
    ....
  36. doi request reprint Centennial paper: Proteomics in animal science
    J D Lippolis
    Periparturient Diseases of Cattle Research Unit, USDA ARS, National Animal Disease Center, Ames, IA 50010, USA
    J Anim Sci 86:2430-41. 2008
    ..The current proteomic research in animal sciences will be discussed, and the potential uses for this technology will be highlighted...
  37. ncbi request reprint Nucleotide sequence of the bovine vitamin D3 receptor
    H L Neibergs
    USDA, National Animal Disease Center, Ames, IA 50010 0070, USA
    J Dairy Sci 79:1313-5. 1996
  38. ncbi request reprint Cloning and sequencing of a cDNA encoding bovine intercellular adhesion molecule 3 (ICAM-3)
    E K Lee
    Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames 50010, USA
    Gene 174:311-3. 1996
    ..The overall identities of the deduced aa sequence with those of human ICAM-3 and ICAM-1 are 61% and 58%, respectively. The predicted number and positions of Cys residues are all conserved between the bovine and human ICAM 3 aa sequences...