K B Register

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. pmc Genomic features of Bordetella parapertussis clades with distinct host species specificity
    Mary M Brinig
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Genome Biol 7:R81. 2006
  2. ncbi request reprint Use of ribotyping to distinguish Bordetella bronchiseptica isolates
    K B Register
    Avian and Swine Respiratory Diseases Research Unit, USDA Agricultural Research Service, Ames, Iowa, USA
    Int J Syst Bacteriol 47:678-83. 1997
  3. ncbi request reprint Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described real-time PCR targeting the pertactin gene
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    J Med Microbiol 56:1608-10. 2007
  4. ncbi request reprint Immune response in mice and swine to DNA vaccines derived from the Pasteurella multocida toxin gene
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research, Service National Animal Disease Center, P O Box 70, Ames, IA 50010, United States
    Vaccine 25:6118-28. 2007
  5. pmc Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA ARS National Animal Disease Center, P O Box 70, 2300 Dayton Road, Ames, IA 50010, USA
    J Clin Microbiol 44:4577-83. 2006
  6. ncbi request reprint Analytical verification of a multiplex PCR for identification of Bordetella bronchiseptica and Pasteurella multocida from swine
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    Vet Microbiol 117:201-10. 2006
  7. pmc Analytical verification of a PCR assay for identification of Bordetella avium
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA ARS National Animal Disease Center, P O Box 70, 2300 Dayton Road, Ames, Iowa 50010, USA
    J Clin Microbiol 43:5567-73. 2005
  8. ncbi request reprint Comparative sequence analysis of Bordetella bronchiseptica pertactin gene (prn) repeat region variants in swine vaccines and field isolates
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, P O Box 70, Ames, IA 50010, USA
    Vaccine 23:48-57. 2004
  9. ncbi request reprint Optimized ribotyping protocol applied to Hungarian Bordetella bronchiseptica isolates: identification of two novel ribotypes
    K B Register
    Avian and Swine Respiratory Diseases Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    Vet Microbiol 69:277-85. 1999
  10. pmc A highly adherent phenotype associated with virulent Bvg+-phase swine isolates of Bordetella bronchiseptica grown under modulating conditions
    K B Register
    National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, Iowa 50010, USA
    Infect Immun 65:5295-300. 1997

Detail Information

Publications25

  1. pmc Genomic features of Bordetella parapertussis clades with distinct host species specificity
    Mary M Brinig
    Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305, USA
    Genome Biol 7:R81. 2006
    ..The disease process in sheep is not well understood, nor are the genetic and transcriptional differences that might provide the basis for host specificity among ovine and human strains...
  2. ncbi request reprint Use of ribotyping to distinguish Bordetella bronchiseptica isolates
    K B Register
    Avian and Swine Respiratory Diseases Research Unit, USDA Agricultural Research Service, Ames, Iowa, USA
    Int J Syst Bacteriol 47:678-83. 1997
    ..There was no association between geographic location and ribotype. The technique which we used may be useful for epidemiologic studies in which the transmission of B. bronchiseptica, both within and between species, is investigated...
  3. ncbi request reprint Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described real-time PCR targeting the pertactin gene
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    J Med Microbiol 56:1608-10. 2007
    ..pertussis using the RT-PCR assay. These data suggest that the use of the assay without confirmatory testing may result in erroneous identification of a significant proportion of human isolates of B. bronchiseptica as B. pertussis...
  4. ncbi request reprint Immune response in mice and swine to DNA vaccines derived from the Pasteurella multocida toxin gene
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research, Service National Animal Disease Center, P O Box 70, Ames, IA 50010, United States
    Vaccine 25:6118-28. 2007
    ..This report documents, for the first time, the ability of a DNA vaccine to elicit immune responses to the P. multocida toxin in both mice and swine...
  5. pmc Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA ARS National Animal Disease Center, P O Box 70, 2300 Dayton Road, Ames, IA 50010, USA
    J Clin Microbiol 44:4577-83. 2006
    ..However, PCR amplicons of the predicted size were detectable in 78% of IS481-negative strains. Our results suggest that PCR targeting IS481 may not be sufficiently specific for reliable identification of B. pertussis...
  6. ncbi request reprint Analytical verification of a multiplex PCR for identification of Bordetella bronchiseptica and Pasteurella multocida from swine
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    Vet Microbiol 117:201-10. 2006
    ..The data presented establish this multiplex PCR as a reliable method for identification of B. bronchiseptica and both toxigenic and nontoxigenic P. multocida that may greatly simplify investigations of swine PAR and bronchopneumonia...
  7. pmc Analytical verification of a PCR assay for identification of Bordetella avium
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA ARS National Animal Disease Center, P O Box 70, 2300 Dayton Road, Ames, Iowa 50010, USA
    J Clin Microbiol 43:5567-73. 2005
    ..Sequence analysis of the B. avium PCR amplicons identified only three occasional sequence polymorphisms. These data indicate the B. avium PCR assay can provide clinically significant results...
  8. ncbi request reprint Comparative sequence analysis of Bordetella bronchiseptica pertactin gene (prn) repeat region variants in swine vaccines and field isolates
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, P O Box 70, Ames, IA 50010, USA
    Vaccine 23:48-57. 2004
    ..No relationship was observed between pertactin type and ribotype...
  9. ncbi request reprint Optimized ribotyping protocol applied to Hungarian Bordetella bronchiseptica isolates: identification of two novel ribotypes
    K B Register
    Avian and Swine Respiratory Diseases Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    Vet Microbiol 69:277-85. 1999
    ..bronchiseptica isolates display limited heterogeneity. The ability to detect additional ribotypes under the modified conditions described in this study strengthens the usefulness of ribotyping as an epidemiologic tool...
  10. pmc A highly adherent phenotype associated with virulent Bvg+-phase swine isolates of Bordetella bronchiseptica grown under modulating conditions
    K B Register
    National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, Iowa 50010, USA
    Infect Immun 65:5295-300. 1997
    ..We propose that a Bvg-repressed adhesin, expressed only by modulated bvg+ strains of B. bronchiseptica, may play a key role in the initial colonization of naturally infected swine...
  11. pmc Reduced virulence of a Bordetella bronchiseptica siderophore mutant in neonatal swine
    K B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, Iowa 50010, USA
    Infect Immun 69:2137-43. 2001
    ..B. bronchiseptica mutants with nonrevertible defects in genes required for alcaligin synthesis may be candidates for evaluation as attenuated, live vaccine strains in conventionally reared pigs...
  12. pmc Two-color hybridization assay for simultaneous detection of Bordetella bronchiseptica and toxigenic Pasteurella multocida from swine
    K B Register
    Avian and Swine Respiratory Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, Iowa 50010, USA
    J Clin Microbiol 36:3342-6. 1998
    ..This technique has wide application for diagnostic and experimental studies...
  13. ncbi request reprint Expression of the dermonecrotic toxin by Bordetella bronchiseptica is not necessary for predisposing to infection with toxigenic Pasteurella multocida
    S L Brockmeier
    Respiratory Diseases of Livestock Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA 50010, USA
    Vet Microbiol 125:284-9. 2007
    ..bronchiseptica by itself, infection with non-DNT-producing strains can still predispose to secondary respiratory infections with P. multocida...
  14. ncbi request reprint Effect of temperature modulation and bvg mutation of Bordetella bronchiseptica on adhesion, intracellular survival and cytotoxicity for swine alveolar macrophages
    S L Brockmeier
    Avian and Swine Respiratory Diseases Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    Vet Microbiol 73:1-12. 2000
    ....
  15. pmc Restriction endonuclease analysis discriminates Bordetella bronchiseptica isolates
    R E Sacco
    USDA Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010, USA
    J Clin Microbiol 38:4387-93. 2000
    ..Based on the results presented herein, the combination of REA and ribotyping should provide valuable information in understanding the molecular epidemiology of B. bronchiseptica infections...
  16. pmc Novel genetic and phenotypic heterogeneity in Bordetella bronchiseptica pertactin
    K B Register
    Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010, USA
    Infect Immun 69:1917-21. 2001
    ..Immunoblotting demonstrated phenotypic heterogeneity in pertactin consistent with the predicted combined sizes of the repeat regions. A revised system for classifying B. bronchiseptica pertactin variants is proposed...
  17. pmc Comparison of ribotyping and restriction enzyme analysis for inter- and intraspecies discrimination of Bordetella avium and Bordetella hinzii
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U S Department of Agriculture, Ames, Iowa 50010, USA
    J Clin Microbiol 41:1512-9. 2003
    ..At least some of the newly recognized B. hinzii isolates have been previously reported to cause disease in poults, suggesting that the pathogenicity of this agent for poultry should be more rigorously examined...
  18. pmc Bordetella bronchiseptica in a paediatric cystic fibrosis patient: possible transmission from a household cat
    K B Register
    USDA Agricultural Research Service National Animal Disease Center, Ames, IA, USA
    Zoonoses Public Health 59:246-50. 2012
    ..Genetic characterization of the isolate and comparison with other isolates of human or feline origin strongly suggest that the kitten was the source of infection...
  19. ncbi request reprint Strain-specific virulence of Bordetella hinzii in poultry
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, P O Box 70, Ames, IA 50010, USA
    Avian Dis 53:50-4. 2009
    ..These results demonstrate that some strains of B. hinzii are virulent in turkey poults and may asymptomatically colonize chicks, and suggest this agent may be of concern to poultry producers...
  20. pmc Evaluation of specificity of BP3385 for Bordetella pertussis detection
    Karen B Register
    Respiratory Diseases of Livestock Research Unit, USDA ARS National Animal Disease Center, P O Box 70, 1920 Dayton Road, Ames, IA 50010, USA
    J Clin Microbiol 48:3334-7. 2010
    ..Our results demonstrate that this gene is also present in some strains of Bordetella hinzii and Bordetella bronchiseptica...
  21. pmc Role of the dermonecrotic toxin of Bordetella bronchiseptica in the pathogenesis of respiratory disease in swine
    Susan L Brockmeier
    Respiratory Diseases of Livestock Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010, USA
    Infect Immun 70:481-90. 2002
    ..Therefore, production of DNT by B. bronchiseptica is necessary to produce the lesions of turbinate atrophy and bronchopneumonia in pigs infected with this organism...
  22. pmc Characterization and comparative analysis of the genes encoding Haemophilus parasuis outer membrane proteins P2 and P5
    Michael A Mullins
    Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, IA 50010, USA
    J Bacteriol 191:5988-6002. 2009
    ..Real-time reverse transcription-PCR indicated that both genes are expressed in all of the strains...
  23. ncbi request reprint Molecular and antigenic characterization of Bordetella bronchiseptica isolated from a wild southern sea otter (Enhydra lutris nereis) with severe suppurative bronchopneumonia
    Clare M Staveley
    California Department of Fish and Game, Marine Wildlife Veterinary Care and Research Center, Santa Cruz, CA 95060, USA
    J Vet Diagn Invest 15:570-4. 2003
    ..bronchiseptica isolate. Ribotype analysis using Pvu II restriction digests indicated that this isolate is most similar to strains commonly obtained in domestic dogs and cats...
  24. ncbi request reprint Prevalence of Bordetella avium infection in selected wild and domesticated birds in the eastern USA
    Thomas R Raffel
    Ohio Wesleyan University, Delaware, Ohio 43015, USA
    J Wildl Dis 38:40-6. 2002
    ..Bordetella avium is present in wild bird populations of multiple species. Transmission from free-living avian populations to domesticated poultry populations may be possible and should be examined...
  25. doi request reprint Persistent Bordetella bronchiseptica pneumonia in an immunocompetent infant and genetic comparison of clinical isolates with kennel cough vaccine strains
    Barbara A Rath
    Department of Pediatrics, Division of Infectious Diseases, Tulane University Health Sciences Center, New Orleans, Louisiana, USA
    Clin Infect Dis 46:905-8. 2008
    ..Genetic analysis demonstrated that the child was not infected with a kennel cough vaccine strain, although the family's dog had recently been vaccinated. The infection cleared with imipenem therapy...