George C Paoli

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. ncbi request reprint Inactivation of avirulent pgm(+) and Δpgm Yersinia pestis by ultraviolet light (UV-C)
    George C Paoli
    Molecular Characterization of Foodborne Pathogens, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA Electronic address
    Food Microbiol 44:168-72. 2014
  2. ncbi request reprint Development of Listeria monocytogenes-specific immunomagnetic beads using a single-chain antibody fragment
    George C Paoli
    Microbial Biophysics and Residue Chemistry Research Unit, United States Department of Agriculture, Agricultural Research Service, Wyndmoor, PA 19038 8598, USA
    Foodborne Pathog Dis 4:74-83. 2007
  3. ncbi request reprint Single-chain Fv antibody with specificity for Listeria monocytogenes
    George C Paoli
    Microbial Biophysics and Residue Chemistry and Core Technologies, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Service, Wyndmoor, PA 19038, USA
    J Immunol Methods 289:147-55. 2004
  4. doi request reprint A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's
    Peter L Irwin
    Molecular Characterization of Foodborne Pathogens, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USA
    Anal Bioanal Chem 402:2713-25. 2012
  5. ncbi request reprint Automated immunomagnetic separation for the detection of Escherichia coli O157:H7 from spinach
    Jing Chen
    MOST USDA Joint Research Center for Food Safety, Department of Food Science and Technology and Bor Luh Food Safety Center, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, China USDA MOST Joint Research Center for Food Safety, Molecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center USDA ARS ERRC, 600 E Mermaid Lane, Wyndmoor, PA 19038, United States
    Int J Food Microbiol 179:33-7. 2014
  6. pmc Phenotypic and Genotypic Characterization of Biofilm Forming Capabilities in Non-O157 Shiga Toxin-Producing Escherichia coli Strains
    Chin Yi Chen
    Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, Pennsylvania, United States of America
    PLoS ONE 8:e84863. 2013
  7. doi request reprint How does Listeria monocytogenes combat acid conditions?
    James L Smith
    Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038 8598, USA
    Can J Microbiol 59:141-52. 2013
  8. doi request reprint DNA extraction protocol for rapid PCR detection of pathogenic bacteria
    Jeffrey D Brewster
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 E Mermaid Lane, Philadelphia, PA 19118, USA Electronic address
    Anal Biochem 442:107-9. 2013
  9. pmc Evidence for a bimodal distribution of Escherichia coli doubling times below a threshold initial cell concentration
    Peter L Irwin
    Molecular Characterization of Foodborne Pathogens Research Unit, Agricultural Research Service, US Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    BMC Microbiol 10:207. 2010
  10. ncbi request reprint SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody
    Viswaprakash Nanduri
    Center For Food Safety and Engineering, Department of Food Science, Purdue University, 745 Agriculture Mall Dr, West Lafayette, IN 47907, USA
    Biosens Bioelectron 23:248-52. 2007

Detail Information

Publications10

  1. ncbi request reprint Inactivation of avirulent pgm(+) and Δpgm Yersinia pestis by ultraviolet light (UV-C)
    George C Paoli
    Molecular Characterization of Foodborne Pathogens, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA Electronic address
    Food Microbiol 44:168-72. 2014
    ..Though strain variations exist, the results showing that UV-C can inactivate Y. pestis cells on agar surfaces suggest that UV-C would be effect in inactivating Y. pestis on food surfaces, particularly foods with a smooth surface. ..
  2. ncbi request reprint Development of Listeria monocytogenes-specific immunomagnetic beads using a single-chain antibody fragment
    George C Paoli
    Microbial Biophysics and Residue Chemistry Research Unit, United States Department of Agriculture, Agricultural Research Service, Wyndmoor, PA 19038 8598, USA
    Foodborne Pathog Dis 4:74-83. 2007
    ..monocytogenes than were observed for the anti-Listeria IMBs (0.05-3.35%); also, the anti-L. monocytogenes scFv-IMBs exhibited improved specificity for L. monocytogenes as determined by cell capture efficiency in pure and mixed cultures...
  3. ncbi request reprint Single-chain Fv antibody with specificity for Listeria monocytogenes
    George C Paoli
    Microbial Biophysics and Residue Chemistry and Core Technologies, US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Service, Wyndmoor, PA 19038, USA
    J Immunol Methods 289:147-55. 2004
    ..This is the first report of a species-specific antibody for viable cells of L. monocytogenes (i.e., the ability to bind to L. monocytogenes without cross-reactivity toward any other species of Listeria)...
  4. doi request reprint A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's
    Peter L Irwin
    Molecular Characterization of Foodborne Pathogens, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USA
    Anal Bioanal Chem 402:2713-25. 2012
    ..This "correction" in the standard-based [DNA](unk) determination is typically 20-60% when the difference in the standard and unknown E is >0.1...
  5. ncbi request reprint Automated immunomagnetic separation for the detection of Escherichia coli O157:H7 from spinach
    Jing Chen
    MOST USDA Joint Research Center for Food Safety, Department of Food Science and Technology and Bor Luh Food Safety Center, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, China USDA MOST Joint Research Center for Food Safety, Molecular Characterization of Foodborne Pathogens Research Unit, United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center USDA ARS ERRC, 600 E Mermaid Lane, Wyndmoor, PA 19038, United States
    Int J Food Microbiol 179:33-7. 2014
    ..Thus, despite the reduced capture efficiencies observed for the Pathatrix methods, the large increase in sample volume results in a greater number of captured cells for downstream detection resulting in improved detection sensitivity...
  6. pmc Phenotypic and Genotypic Characterization of Biofilm Forming Capabilities in Non-O157 Shiga Toxin-Producing Escherichia coli Strains
    Chin Yi Chen
    Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, Pennsylvania, United States of America
    PLoS ONE 8:e84863. 2013
    ..Understanding biofilm regulatory mechanisms will prove beneficial in reducing pathogen survival and enhancing food safety. ..
  7. doi request reprint How does Listeria monocytogenes combat acid conditions?
    James L Smith
    Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038 8598, USA
    Can J Microbiol 59:141-52. 2013
    ..There is a need to study other acid resistance mechanisms of L. monocytogenes to determine their effectiveness in protecting the organism in acidic foods or during transit through the acid stomach...
  8. doi request reprint DNA extraction protocol for rapid PCR detection of pathogenic bacteria
    Jeffrey D Brewster
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 600 E Mermaid Lane, Philadelphia, PA 19118, USA Electronic address
    Anal Biochem 442:107-9. 2013
    ..The 5× HotSHOT+Tween reagent exhibited minimal inhibition and high extraction efficiency for both test organisms, providing a 15-min single-tube DNA-extraction protocol suitable for highly sensitive quantitative PCR assays. ..
  9. pmc Evidence for a bimodal distribution of Escherichia coli doubling times below a threshold initial cell concentration
    Peter L Irwin
    Molecular Characterization of Foodborne Pathogens Research Unit, Agricultural Research Service, US Department of Agriculture, Eastern Regional Research Center, Wyndmoor, PA 19038, USA
    BMC Microbiol 10:207. 2010
    ..Below this cell concentration (<or= 100 -1,000 CFU mL-1 ; <or= 27-270 CFU well-1) we observed an obvious increase in the tau scatter...
  10. ncbi request reprint SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody
    Viswaprakash Nanduri
    Center For Food Safety and Engineering, Department of Food Science, Purdue University, 745 Agriculture Mall Dr, West Lafayette, IN 47907, USA
    Biosens Bioelectron 23:248-52. 2007
    ..monocytogenes whole cells was estimated to be 2 x 10(6)cfu/ml. The sensor was also used to determine the dissociation constant (Kd) for the interaction of phage-displayed scFv and soluble ActA in solution as 4.5 nM...