Robert W Li

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. pmc Worm burden-dependent disruption of the porcine colon microbiota by Trichuris suis infection
    Sitao Wu
    Center for Research in Biological Systems, University of California San Diego, San Diego, California, United States of America
    PLoS ONE 7:e35470. 2012
  2. pmc Alternative splicing regulated by butyrate in bovine epithelial cells
    Sitao Wu
    Center for Research in Biological Systems, University of California San Diego, San Diego, California, United States of America
    PLoS ONE 7:e39182. 2012
  3. doi request reprint Exploring the host transcriptome for mechanisms underlying protective immunity and resistance to nematode infections in ruminants
    Robert W Li
    USDA ARS, Bovine Functional Genomics Laboratory, Beltsville, MD, USA
    Vet Parasitol 190:1-11. 2012
  4. pmc Alterations in the porcine colon microbiota induced by the gastrointestinal nematode Trichuris suis
    Robert W Li
    USDA ARS ANRI, Bovine Functional Genomics Laboratory, Beltsville, Maryland, USA
    Infect Immun 80:2150-7. 2012
  5. doi request reprint Cytoskeleton remodeling and alterations in smooth muscle contractility in the bovine jejunum during nematode infection
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, USDA ARS, Beltsville, MD 20705, USA
    Funct Integr Genomics 12:35-44. 2012
  6. pmc Perturbation dynamics of the rumen microbiota in response to exogenous butyrate
    Robert W Li
    United States Department of Agriculture Agricultural Research Service, Bovine Functional Genomics Laboratory, Beltsville, Maryland, United States of America
    PLoS ONE 7:e29392. 2012
  7. doi request reprint Characterization of the rumen microbiota of pre-ruminant calves using metagenomic tools
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
    Environ Microbiol 14:129-39. 2012
  8. pmc Infection with the gastrointestinal nematode Ostertagia ostertagi in cattle affects mucus biosynthesis in the abomasum
    Manuela Rinaldi
    Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Vet Res 42:61. 2011
  9. pmc Genome wide analysis of the bovine mucin genes and their gastrointestinal transcription profile
    Prisca R Hoorens
    Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    BMC Genomics 12:140. 2011
  10. doi request reprint Localized complement activation in the development of protective immunity against Ostertagia ostertagi infections in cattle
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Beltsville, MD 20705, USA
    Vet Parasitol 174:247-56. 2010

Detail Information

Publications40

  1. pmc Worm burden-dependent disruption of the porcine colon microbiota by Trichuris suis infection
    Sitao Wu
    Center for Research in Biological Systems, University of California San Diego, San Diego, California, United States of America
    PLoS ONE 7:e35470. 2012
    ..Our findings provided insight into the dynamics of the proximal colon microbiota in pigs in response to T. suis infection...
  2. pmc Alternative splicing regulated by butyrate in bovine epithelial cells
    Sitao Wu
    Center for Research in Biological Systems, University of California San Diego, San Diego, California, United States of America
    PLoS ONE 7:e39182. 2012
    ..Our results provided a snapshot into complex transcriptome dynamics regulated by butyrate, which will facilitate our understanding of the biological effects of butyrate and other HDAC inhibitors...
  3. doi request reprint Exploring the host transcriptome for mechanisms underlying protective immunity and resistance to nematode infections in ruminants
    Robert W Li
    USDA ARS, Bovine Functional Genomics Laboratory, Beltsville, MD, USA
    Vet Parasitol 190:1-11. 2012
    ..This review summarizes recent progress and limitations of understanding regulatory genetic networks and biological pathways that affect host resistance and susceptibility to nematode infection in ruminants...
  4. pmc Alterations in the porcine colon microbiota induced by the gastrointestinal nematode Trichuris suis
    Robert W Li
    USDA ARS ANRI, Bovine Functional Genomics Laboratory, Beltsville, Maryland, USA
    Infect Immun 80:2150-7. 2012
    ..Our findings should facilitate development of strategies for parasitic control in pigs and humans...
  5. doi request reprint Cytoskeleton remodeling and alterations in smooth muscle contractility in the bovine jejunum during nematode infection
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, USDA ARS, Beltsville, MD 20705, USA
    Funct Integr Genomics 12:35-44. 2012
    ..Together, these data suggest that smooth muscle hypercontractility may be initiated as a result of a primary C. oncophora infection, which may represent a mechanism for host responses in the jejunum during nematode infection...
  6. pmc Perturbation dynamics of the rumen microbiota in response to exogenous butyrate
    Robert W Li
    United States Department of Agriculture Agricultural Research Service, Bovine Functional Genomics Laboratory, Beltsville, Maryland, United States of America
    PLoS ONE 7:e29392. 2012
    ..Our findings provide insight into perturbation dynamics of the rumen microbial ecosystem and should guide efforts in formulating optimal uses of probiotic bacteria treating human diseases...
  7. doi request reprint Characterization of the rumen microbiota of pre-ruminant calves using metagenomic tools
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
    Environ Microbiol 14:129-39. 2012
    ..Our results provide insight into rumen microbiota dynamics and will facilitate efforts in formulating optimal early-weaning strategies...
  8. pmc Infection with the gastrointestinal nematode Ostertagia ostertagi in cattle affects mucus biosynthesis in the abomasum
    Manuela Rinaldi
    Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Vet Res 42:61. 2011
    ....
  9. pmc Genome wide analysis of the bovine mucin genes and their gastrointestinal transcription profile
    Prisca R Hoorens
    Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    BMC Genomics 12:140. 2011
    ..The aim of the current study was to identify all members of the bovine mucin family by genome mining and subsequently investigate the transcription pattern of these mucins in the GI tract...
  10. doi request reprint Localized complement activation in the development of protective immunity against Ostertagia ostertagi infections in cattle
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Beltsville, MD 20705, USA
    Vet Parasitol 174:247-56. 2010
    ..These results are the first evidence that local complement activation may be involved in the development of long-term protective immunity and provide a novel mechanistic insight into resistance against O. ostertagi in cattle...
  11. pmc Characterization of the abomasal transcriptome for mechanisms of resistance to gastrointestinal nematodes in cattle
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, USDA ARS, Beltsville, MD 20705, USA
    Vet Res 42:114. 2011
    ..Our results provide insights into the development of host immunity to gastrointestinal nematode infection and will facilitate understanding of mechanism underlying host resistance...
  12. pmc Metagenome plasticity of the bovine abomasal microbiota in immune animals in response to Ostertagia ostertagi infection
    Robert W Li
    Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, United States of America
    PLoS ONE 6:e24417. 2011
    ..A minimal disruption in the bovine abomasal microbiota by reinfection may contribute equally to the restoration of gastric function in immune animals...
  13. pmc The vitamin D receptor and inducible nitric oxide synthase associated pathways in acquired resistance to Cooperia oncophora infection in cattle
    Robert W Li
    Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
    Vet Res 42:48. 2011
    ..Our findings facilitate the understanding of molecular mechanisms underlying the development of acquired resistance, which could have an important implication in vaccine design...
  14. ncbi request reprint Canonical pathways and networks regulated by estrogen in the bovine mammary gland
    Robert W Li
    Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA
    Funct Integr Genomics 8:55-68. 2008
    ....
  15. doi request reprint A temporal shift in regulatory networks and pathways in the bovine small intestine during Cooperia oncophora infection
    Robert W Li
    Bovine Functional Genomics Laboratory, U S Department of Agriculture Agricultural Research Service, BARC East, Beltsville, MD 20705, USA
    Int J Parasitol 39:813-24. 2009
    ..oncophora. These results provide a potential molecular roadmap for future studies aimed at defining host immune responses and understanding protective immunity against gastrointestinal nematodes...
  16. ncbi request reprint Mucin biosynthesis in the bovine goblet cell induced by Cooperia oncophora infection
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, USDA ARS, Beltsville, MD 20705, USA
    Vet Parasitol 165:281-9. 2009
    ....
  17. doi request reprint A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis
    Manuela Rinaldi
    USDA ARS, Beltsville, MD 20705, USA
    Funct Integr Genomics 10:21-38. 2010
    ..Data support an important sentinel function for the teat, as these tissues responded rapidly and intensely, with production of cytokines and antimicrobial peptides...
  18. doi request reprint Enhanced mitochondrial complex gene function and reduced liver size may mediate improved feed efficiency of beef cattle during compensatory growth
    Erin E Connor
    Beltsville Agricultural Research Center, ARS, USDA, MD 20705, USA
    Funct Integr Genomics 10:39-51. 2010
    ....
  19. pmc Transcriptome characterization by RNA-seq unravels the mechanisms of butyrate-induced epigenomic regulation in bovine cells
    Sitao Wu
    Center for Research in Biological Systems, University of California San Diego, San Diego, California, United States of America
    PLoS ONE 7:e36940. 2012
    ..Our results provided insight into the transcriptome alterations induced by butyrate, which will undoubtedly facilitate our understanding of the molecular mechanisms underlying butyrate-induced epigenomic regulation in bovine cells...
  20. ncbi request reprint Identification of estrogen-responsive genes in the parenchyma and fat pad of the bovine mammary gland by microarray analysis
    Robert W Li
    Bovine Functional Genomics Laboratory, United States Department of Agriculture Agricultural Research Service, Beltsville, Maryland 20705, USA
    Physiol Genomics 27:42-53. 2006
    ..The distinctive expression patterns regulated by estrogen in parenchyma and fat pad shed light on the need for both tissues to obtain normal mammary development...
  21. doi request reprint Gene expression in bovine rumen epithelium during weaning identifies molecular regulators of rumen development and growth
    Erin E Connor
    Bovine Functional Genomics Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 306, BARC East, Beltsville, MD 20705, USA
    Funct Integr Genomics 13:133-42. 2013
    ....
  22. doi request reprint Genome-wide ChIP-seq mapping and analysis reveal butyrate-induced acetylation of H3K9 and H3K27 correlated with transcription activity in bovine cells
    Joo Heon Shin
    Lieber Institute for Brain Development, Johns Hopkins University, 855 North Wolfe Street, Suite 102, Baltimore, MD 21205, USA
    Funct Integr Genomics 12:119-30. 2012
    ..Our results reveal that butyrate-induced acetylation in H3K9 and H3K27 changes the sequence-based binding preference of histone H3 and underlies the potential mechanisms of gene expression regulation induced by butyrate...
  23. ncbi request reprint Local inflammation as a possible mechanism of resistance to gastrointestinal nematodes in Angus heifers
    Robert W Li
    Bovine Functional Genomics Laboratory, USDA ARS, Beltsville, MD 20705, USA
    Vet Parasitol 145:100-7. 2007
    ..These data indicate that resistant animals can better maintain inflammatory responses at the site of infection, suggesting a possible novel mechanism of resistance...
  24. pmc Butyrate induces profound changes in gene expression related to multiple signal pathways in bovine kidney epithelial cells
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture Agricultural Research Service, Beltsville, MD 20705, USA
    BMC Genomics 7:234. 2006
    ..These oligonucleotides represent approximately 45,383 unique cattle sequences...
  25. pmc Production and utilization of a high-density oligonucleotide microarray in channel catfish, Ictalurus punctatus
    Robert W Li
    USDA, Agricultural Research Service, Catfish Genetics Research Unit, Stoneville, MS 38776, USA
    BMC Genomics 7:134. 2006
    ..This platform was used to profile gene expression in catfish spleens 2 h, 4 h, 8 h and 24 h after injection of lipopolysaccharide (LPS)...
  26. ncbi request reprint Lipopolysaccharide-induced early response genes in bovine peripheral blood mononuclear cells implicate GLG1/E-selectin as a key ligand-receptor interaction
    Cong jun Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U S Department of Agriculture, BARC East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Funct Integr Genomics 9:335-49. 2009
    ..In particular, gene expression data identified Golgi complex-localized glycoprotein 1/endothelial-selectin as a key ligand-receptor interaction in the early response of cells...
  27. pmc Quantification of Transcriptome Responses of the Rumen Epithelium to Butyrate Infusion using RNA-seq Technology
    Ransom L Baldwin
    USDA ARS, Bovine Functional Genomics Laboratory, Beltsville, MD, USA
    Gene Regul Syst Bio 6:67-80. 2012
    ....
  28. pmc Analysis of copy number variations among diverse cattle breeds
    George E Liu
    USDA ARS, ANRI, Bovine Functional Genomics Laboratory, Beltsville, Maryland 20705, USA
    Genome Res 20:693-703. 2010
    ..Our results provide a valuable resource beyond microsatellites and single nucleotide polymorphisms to explore the full dimension of genetic variability for future cattle genomic research...
  29. doi request reprint Identification of conserved regulatory elements in mammalian promoter regions: a case study using the PCK1 promoter
    George E Liu
    Bovine Functional Genomics Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA
    Genomics Proteomics Bioinformatics 6:129-43. 2008
    ..Our full set of TFBS predictions is freely available at http://bfgl.anri.barc.usda.gov/tfbsConsSites...
  30. ncbi request reprint Pathway analysis identifies perturbation of genetic networks induced by butyrate in a bovine kidney epithelial cell line
    Cong jun Li
    Growth Biology Laboratory, Animal and Natural Resources Institute ARS, U S Derpartment of Agriculture, 10300 Baltimore Avenue, BARC East, Beltsville, MD 20705, USA
    Funct Integr Genomics 7:193-205. 2007
    ..The present findings provide a basis for understanding the full range of the biological roles and the molecular mechanisms that butyrate may play in animal cell growth, proliferation, and energy metabolisms...
  31. doi request reprint Characteristics of peri-implantation porcine concepti population and maternal milieu influence the transcriptome profile
    Le Ann Blomberg
    Animal Biosciences and Biotechnology Laboratory, USDA Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, MD 20705, USA
    Mol Reprod Dev 77:978-89. 2010
    ..Also highlighted were ways by which the conceptus' microenvironment might be affected and developmental factors that may be of interest to interrogate further to determine if, and how, they affect embryo competency/elongation...
  32. pmc Butyrate Induced IGF2 Activation Correlated with Distinct Chromatin Signatures Due to Histone Modification
    Joo Heon Shin
    Lieber Institute for Brain Development, Johns Hopkins University, Baltimore, Maryland, United States of America
    Gene Regul Syst Bio 7:57-70. 2013
    ..Our results provided insights into the mechanism of butyrate-induced loss of imprinting (LOI) of IGF2 and regulation of gene expression by histone modification...
  33. doi request reprint Characterization of the longissimus lumborum transcriptome response to adding propionate to the diet of growing Angus beef steers
    Ransom L Baldwin
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture Agricultural Research Service, Beltsville, Maryland 20705, USA
    Physiol Genomics 44:543-50. 2012
    ..lipolytic activities)...
  34. doi request reprint Comparative proteomic and regulatory network analyses of the elongating pig conceptus
    Séverine A Degrelle
    USDA Agricultural Research Service, Animal and Natural Resources Institute, Animal Biosciences and Biotechnology Laboratory, Beltsville, MD, USA
    Proteomics 9:2678-94. 2009
    ..This proteomic study helps to elucidate the biological mechanisms underlying the early embryonic development of the pig...
  35. pmc Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells
    Cong jun Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, ARS, USDA, 10300 Baltimore Ave, Beltsville, MD 20705
    Gene Regul Syst Bio 6:1-14. 2012
    ..We concluded that α-tocopherol pre-treatment has a significant antagonistic effect that modulates the cell's response to the TNF-α challenge by altering the gene expression activities of some important signaling molecules...
  36. doi request reprint Transcriptomic alterations in human prostate cancer cell LNCaP tumor xenograft modulated by dietary phenethyl isothiocyanate
    Robert W Li
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Bovine Functional Genomics Laboratory, Beltsville, MD, USA
    Mol Carcinog 52:426-37. 2013
    ..This study represents the first use of RNAseq to analyze tumors from animals consuming dietary phenethyl isothiocyanate and to identify potential molecular signatures that may explain the cancer protective effect of this compound...
  37. pmc Comparison of the transcriptomes of long-term label retaining-cells and control cells microdissected from mammary epithelium: an initial study to characterize potential stem/progenitor cells
    Ratan K Choudhary
    Department of Animal and Avian Sciences, University of Maryland College Park, MD, USA
    Front Oncol 3:21. 2013
    ..Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study...
  38. ncbi request reprint Genomic organisation and expression of the natural killer cell enhancing factor (NKEF) gene in channel catfish, Ictalurus punctatus (Rafinesque)
    Robert W Li
    USDA, Agricultural Research Service, Catfish Genetics Research Unit, Stoneville, MS 38776, USA
    Fish Shellfish Immunol 20:72-82. 2006
    ..1 cM from locus IpCG0177 (r=0.05; LOD=45.8), 6.0 cM from a novel immune type receptor, syntenic (40 cM) with T-cell receptor alpha, and not linked with MHC loci...
  39. doi request reprint Neospora caninum: comparative gene expression profiling of Neospora caninum wild type and a temperature sensitive clone
    Robert W Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, USDA ARS, Beltsville, MD 20705, United States
    Exp Parasitol 129:346-54. 2011
    ....
  40. pmc Alpha-Tocopherol Modulates Transcriptional Activities that Affect Essential Biological Processes in Bovine Cells
    Cong jun Li
    Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, ARS, USDA, 10300 Baltimore Ave, Beltsville, MD, 20705, USA
    Gene Regul Syst Bio 4:109-24. 2010
    ..The data also provided direct evidence that α-tocopherol is involved in maintaining immuno-homeostasis through targeting the C3 (Complement Component 3) gene...