Research Topics
| Dolores HillSummaryAffiliation: Agricultural Research Service Country: USA Publications
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Detail Information
Publications
Toxoplasma gondii prevalence in farm animals in the United StatesD E Hill
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center Animal Parasitic Diseases Laboratory, Beltsville, MD 20705, USA
Int J Parasitol 43:107-13. 2013..Toxoplasma infection is common in food animals in the United States. Implementation of management factors such as biosecure confinement housing are important in reducing the levels of infection in animals destined for human consumption...
Identification of a sporozoite-specific antigen from Toxoplasma gondiiDolores Hill
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705, USA
J Parasitol 97:328-37. 2011..These data suggest that TgERP may be useful in detecting exposure to sporozoites in early T. gondii infection and implicates oocysts as the agent of infection...
Survival of North American genotypes of Trichinella in frozen porkD E Hill
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East, Beltsville, Maryland 20705, USA
J Food Prot 72:2565-70. 2009..These data close a gap in knowledge about the effectiveness of freezing for inactivating these parasites in pork and should alleviate concern about the safety of frozen pork products from the United States...
Cessation of Trichinella spiralis transmission among scavenging mammals after the removal of infected pigs from a poorly managed farm: implications for trichinae transmission in the USD E Hill
Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
Zoonoses Public Health 57:e116-23. 2010..Results of the study suggest that T. spiralis, typically transmitted in the peridomestic rat-pig-human cycle in the US, was not maintained in scavenging mammals in the absence of infected pigs...
Seroprevalence of and risk factors for Toxoplasma gondii in the US swine herd using sera collected during the National Animal Health Monitoring Survey (Swine 2006)D E Hill
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East, Beltsville, MD 20705, USA
Zoonoses Public Health 57:53-9. 2010..Production practices which eliminate these sources of exposure can reduce the risk of Toxoplasma infection in pigs, and reduce the likelihood of human infection from consumption of infected pork...
Isolation of Neospora caninum from naturally infected white-tailed deer (Odocoileus virginianus)M C B Vianna
United States Department of Agriculture, Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, Beltsville Agricultural Research Center, Building 1001, Beltsville, MD 20705, USA
Vet Parasitol 129:253-7. 2005..This is the first record of in vitro isolation of N. caninum from white-tailed deer and lends credence to the white-tailed deer as an intermediate host for this parasite...
Differential detection of Hammondia hammondi from Toxoplasma gondii using polymerase chain reactionC Sreekumar
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
Parasitol Int 54:267-9. 2005..hammondi from T. gondii even in samples containing both parasites, thus obviating the need for other labourious techniques like mouse bioassay and in vitro cultivation...
Specific detection of Neospora caninum oocysts in fecal samples from experimentally-infected dogs using the polymerase chain reactionD E Hill
USDA, ARS, LPSI, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705, USA
J Parasitol 87:395-8. 2001..The assay should be useful in epidemiological surveys of both domestic and wild canine hosts and in investigations of oocyst biology in experimental infections...
Trichinella pseudospiralis from a wild pig in TexasH R Gamble
U.S. Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, BARC-East, Beltsville, MD 20705, USA. rgamble2nas.edu
Vet Parasitol 132:147-50. 2005..This is the second report of T. pseudospiralis in the United States and the first report of the parasite in a food animal species in the U.S...
Genotyping and detection of multiple infections of Toxoplasma gondii using PyrosequencingC Sreekumar
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA
Int J Parasitol 35:991-9. 2005..We conclude that Pyrosequencing offers a simple, rapid and efficient means for diagnosis and genotyping of T. gondii, as well as detection and quantification of multiple genotype infections of T. gondii...
Establishment of Besnoitia darlingi from opossums (Didelphis virginiana) in experimental intermediate and definitive hosts, propagation in cell culture, and description of ultrastructural and genetic characteristicsJ P Dubey
Parasite Biology Laboratory, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, United States Department of Agriculture, Building 1001, BARC East, Beltsville, MD 20705 2350, USA
Int J Parasitol 32:1053-64. 2002..A close relationship to B. besnoiti and an even closer relationship to B. jellisoni was indicated for B. darlingi on the basis of the small subunit and ITS-1 portions of nuclear ribosomal DNA...
Redescription of Neospora caninum and its differentiation from related coccidiaJ P Dubey
Parasite Biology Laboratory, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, United States Department of Agriculture, Building 1001, BARC East, Beltsville, MD 20705 2350, USA
Int J Parasitol 32:929-46. 2002..In this article, the authors redescribe the parasite, distinguish it from related coccidia, and provide accession numbers to its type specimens deposited in museums...
Biological and genetic characterisation of Toxoplasma gondii isolates from chickens (Gallus domesticus) from São Paulo, Brazil: unexpected findingsJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Parasite Biology, Epidemiology and Systematics Laboratory, Beltsville, MD 20705 2350, USA
Int J Parasitol 32:99-105. 2002..gondii isolates. This is the first report of isolation of predominantly type I strains of T. gondii from a food animal. Epidemiological implications of these findings are discussed...
Trichinella murrelli in scavenging mammals from south-central Wisconsin, USAD E Hill
US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory BARC East, Beltsville, Maryland 20705, USA
J Wildl Dis 44:629-35. 2008..These findings extend the geographic range of T. murrelli into Wisconsin, USA...
Endemic toxoplasmosis in pigs on a farm in Maryland: isolation and genetic characterization of Toxoplasma gondiiJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 94:36-41. 2008..Both genotypes 1 and 2 are considered to belong to the clonal type II lineages. Genotype 3 and 4 are nonclonal isolates. Results document high prevalence of T. gondii in pigs on a farm in Maryland...
Neosporosis in Beagle dogs: clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninumJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agricultural, Building 1001, Beltsville, MD 20705, USA
Vet Parasitol 149:158-66. 2007..caninum antibody titers were still persistent in these pups at 23 months of age. Results indicate that medication with Clindamycin can improve clinical condition but not eliminate N. caninum infection...
Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissueD E Hill
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East Beltsville, MD 20705, USA
Vet Parasitol 146:102-6. 2007....
Larval viability and serological response in horses with long-term Trichinella spiralis infectionD E Hill
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East Beltsville, MD 20705, USA
Vet Parasitol 146:107-16. 2007..i. and the persistence of infective muscle larvae for at least 1 year, parasite recovery methods are currently the only suitable detection assays for both meat inspection and epidemiological studies of Trichinella infection in the horse...
Effects of time and temperature on the viability of Toxoplasma gondii tissue cysts in enhanced pork loinD E Hill
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East, Beltsville, Maryland 20705, USA
J Food Prot 69:1961-5. 2006..gondii tissue cysts in pork loins and that storage at meat case temperatures at or below 0 degrees C (32 degrees F) for 7 days also killed T. gondii tissue cysts in pork loins...
A Trichinella murrelli infection in a domestic dog in the United StatesJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agricultural, Building 1001, Beltsville, MD 20705, USA
Vet Parasitol 137:374-8. 2006..murrelli; the isolate was assigned the ISS code: ISS1608 by the International Trichinella Reference Centre. This is the first report of T. murrelli infection in a companion animal...
Effect of commonly used enhancement solutions on the viability of Toxoplasma gondii tissue cysts in pork loinD E Hill
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East, Beltsville, Maryland 20705, USA
J Food Prot 67:2230-3. 2004..4% potassium or sodium lactate, alone or in combination with other components, prevented transmission of T. gondii to cats...
Molecular and biological characterization of Hammondia heydorni-like oocysts from a dog fed hearts from naturally infected white-tailed deer (Odocoileus Virginianus)J P Dubey
Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
J Parasitol 90:1174-6. 2004..heydorni DNA in the samples. The oocysts were considered to be H. heydorni on the basis of their morphology, biology, and molecular characteristics. This is the first record of a H. heydorni-like parasite in the white-tailed deer...
Hammondia heydorni: evidence of genetic diversity among isolates from dogsC Sreekumar
Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
Exp Parasitol 107:65-71. 2004..heydorni. This diversity should be taken into consideration while attempting to elucidate the systematics, diagnostics, and biology of H. heydorni in relation to N. caninum...
Biologic, morphologic, and molecular characterisation of Neospora caninum isolates from littermate dogsJ P Dubey
US Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, BARC East, Building 1040, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
Int J Parasitol 34:1157-67. 2004..caninum tissue cysts from the brain of one dog. Tissue cysts were infective orally to mice and gerbils, but not to cats and dogs. The isolates were also identified as N. caninum by PCR and sequence analysis...
Trichinella nativa in a black bear from Plymouth, New HampshireD E Hill
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1044, BARC East, Beltsville, MD 20705, USA
Vet Parasitol 132:143-6. 2005..Multiplex PCR using genomic DNA from these parasite samples demonstrated that both isolates were T. nativa. This is the first report of the freeze-resistant species, T. nativa, within the continental United States...
Isolation, tissue distribution, and molecular characterization of Toxoplasma gondii from chickens in Grenada, West IndiesJ R Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 91:557-60. 2005..Phenotypically, all isolates from Grenada were avirulent for mice, whereas those from Brazil were mouse-virulent. This is the first report of isolation of T. gondii from chickens from Grenada, West Indies...
Detection of Hammondia heydorni-like organisms and their differentiation from Neospora caninum using random-amplified polymorphic DNA-polymerase chain reactionC Sreekumar
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
J Parasitol 89:1082-5. 2003..It is concluded that H. heydorni and N. caninum are genetically distinct and that such tools may be useful for more detailed characterization of the diversity of related parasites occurring in dogs...
Prevalence of viable Toxoplasma gondii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumersJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
J Parasitol 91:1082-93. 2005..Nevertheless, consumers, especially pregnant women, should be aware that they can acquire T. gondii infection from ingestion of undercooked meat, and in particular, pork. Cooking meat to an internal temperature of 66 C kills T. gondii...
Alterations in the porcine colon microbiota induced by the gastrointestinal nematode Trichuris suisRobert W Li
USDA ARS ANRI, Bovine Functional Genomics Laboratory, Beltsville, Maryland, USA
Infect Immun 80:2150-7. 2012..Our findings should facilitate development of strategies for parasitic control in pigs and humans...
Identification and characterisation of a cDNA sequence encoding a glutamic acid-rich protein specifically transcribed in Trichinella spiralis newborn larvae and recognised by infected swine serumDante S Zarlenga
Immunology and Disease Resistance Laboratory, USDA, ARS, ANRI, Beltsville, MD 20705, USA
Int J Parasitol 32:1361-70. 2002..Mouse antibodies to recombinant antigen localised the glutamic acid-rich proteins to the periphery of the developing stichocyte cells within the newborn larvae consistent with the hypothesis that the newborn larval antigens are secreted...
