Robert L Harrison

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. doi request reprint Classification, genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia, Europe, and North America
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, MD 20705, USA Electronic address
    J Invertebr Pathol 116:27-35. 2014
  2. doi request reprint Concentration- and time-response characteristics of plaque isolates of Agrotis ipsilon multiple nucleopolyhedrovirus derived from a field isolate
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA Agricultural Research Service, Beltsville, MD 20705, USA
    J Invertebr Pathol 112:159-61. 2013
  3. pmc Proteases as insecticidal agents
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, Beltsville, Maryland 20705, USA
    Toxins (Basel) 2:935-53. 2010
  4. doi request reprint Genetic variation and virulence of Autographa californica multiple nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus isolates
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA Agricultural Research Service, Beltsville, MD 20705, USA
    J Invertebr Pathol 110:33-47. 2012
  5. doi request reprint Genomic sequence analysis of a granulovirus isolated from the Old World bollworm, Helicoverpa armigera
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA, Agricultural Research Service, Building 011A, Room 214, BARC W, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Virus Genes 36:565-81. 2008
  6. doi request reprint Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, Building 011A, Room 214, BARC W, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA
    Virus Genes 38:155-70. 2009
  7. doi request reprint Structural divergence among genomes of closely related baculoviruses and its implications for baculovirus evolution
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, PSI, Building 011A, Room 214, BARC W, Beltsville, MD 20705, USA
    J Invertebr Pathol 101:181-6. 2009
  8. doi request reprint Autographa californica multiple nucleopolyhedrovirus ODV-E56 envelope protein is required for oral infectivity and can be substituted functionally by Rachiplusia ou multiple nucleopolyhedrovirus ODV-E56
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service USDA ARS, Plant Sciences Institute, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    J Gen Virol 91:1173-82. 2010
  9. doi request reprint New cell lines derived from the black cutworm, Agrotis ipsilon, that support replication of the A. ipsilon multiple nucleopolyhedrovirus and several group I nucleopolyhedroviruses
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA, Agricultural Research Service, BARC W, Beltsville, MD 20705, USA
    J Invertebr Pathol 99:28-34. 2008
  10. ncbi request reprint Genomic sequence analysis of a nucleopolyhedrovirus isolated from the diamondback moth, Plutella xylostella
    Robert L Harrison
    Insect Biocontrol Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, Building 011A, Room 214, BARC W, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Virus Genes 35:857-73. 2007

Detail Information

Publications25

  1. doi request reprint Classification, genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia, Europe, and North America
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, MD 20705, USA Electronic address
    J Invertebr Pathol 116:27-35. 2014
    ..This study expands our knowledge about genetic variation among LdMNPV isolates and provides novel information on the distinct groups in which these NPVs occur. ..
  2. doi request reprint Concentration- and time-response characteristics of plaque isolates of Agrotis ipsilon multiple nucleopolyhedrovirus derived from a field isolate
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA Agricultural Research Service, Beltsville, MD 20705, USA
    J Invertebr Pathol 112:159-61. 2013
    ..Mixed infections with isolates representing the two different egt genotypes caused the same level of mortality as the field isolate and a plaque isolate with a wild-type egt gene...
  3. pmc Proteases as insecticidal agents
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, Beltsville, Maryland 20705, USA
    Toxins (Basel) 2:935-53. 2010
    ..The sites of protease toxic activity range from the insect midgut to the hemocoel (body cavity) to the cuticle. This review discusses these insecticidal proteases along with their evaluation and use as potential pesticides...
  4. doi request reprint Genetic variation and virulence of Autographa californica multiple nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus isolates
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA Agricultural Research Service, Beltsville, MD 20705, USA
    J Invertebr Pathol 110:33-47. 2012
    ....
  5. doi request reprint Genomic sequence analysis of a granulovirus isolated from the Old World bollworm, Helicoverpa armigera
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA, Agricultural Research Service, Building 011A, Room 214, BARC W, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Virus Genes 36:565-81. 2008
    ..Alignment of the HearGV and XecnGV genome sequences revealed that HearGV is missing approximately 16.6 kbp of XecnGV-homologous sequence and contains approximately 8.2 kbp of sequence not found in the XecnGV genome...
  6. doi request reprint Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, Building 011A, Room 214, BARC W, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA
    Virus Genes 38:155-70. 2009
    ..The deletion in egt was not associated with differences in budded virus or occluded virus production among AgipMNPV clones in cell culture...
  7. doi request reprint Structural divergence among genomes of closely related baculoviruses and its implications for baculovirus evolution
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, PSI, Building 011A, Room 214, BARC W, Beltsville, MD 20705, USA
    J Invertebr Pathol 101:181-6. 2009
    ..These observations suggest an important role for recombination in the early evolution and biological characteristics of baculoviruses of these two groups...
  8. doi request reprint Autographa californica multiple nucleopolyhedrovirus ODV-E56 envelope protein is required for oral infectivity and can be substituted functionally by Rachiplusia ou multiple nucleopolyhedrovirus ODV-E56
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service USDA ARS, Plant Sciences Institute, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    J Gen Virol 91:1173-82. 2010
    ..This result indicated that replacement of the AcMNPV odv-e56 gene with the RoMNPV orthologue did not increase virulence against these two species...
  9. doi request reprint New cell lines derived from the black cutworm, Agrotis ipsilon, that support replication of the A. ipsilon multiple nucleopolyhedrovirus and several group I nucleopolyhedroviruses
    Robert L Harrison
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA, Agricultural Research Service, BARC W, Beltsville, MD 20705, USA
    J Invertebr Pathol 99:28-34. 2008
    ..Our results suggest these new lines can be useful in the study of AgipMNPV and A. ipsilon cellular and molecular biology...
  10. ncbi request reprint Genomic sequence analysis of a nucleopolyhedrovirus isolated from the diamondback moth, Plutella xylostella
    Robert L Harrison
    Insect Biocontrol Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, Building 011A, Room 214, BARC W, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Virus Genes 35:857-73. 2007
    ..3%) of amino acid sequence identity with the AcMNPV-C6 ie-2 product. PlxyMNPV-CL3 apparently acquired this variant ie2 gene by recombination with an undescribed nucleopolyhedrovirus...
  11. doi request reprint Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus
    Robert L Harrison
    Agricultural Research Service, Plant Sciences Institute, Beltsville, MD 20705, USA
    J Gen Virol 89:775-90. 2008
    ..Variant genotypes with deletions extending around the egt gene have been reported previously from a Nicaraguan field isolate of SfMNPV, suggesting that the presence of such variants is a common feature of SfMNPV populations...
  12. doi request reprint Genetic and biological variation among nucleopolyhedrovirus isolates from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)
    Daniel L Rowley
    Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Virus Genes 40:458-68. 2010
    ....
  13. doi request reprint Determination and analysis of the genome sequence of Spodoptera littoralis multiple nucleopolyhedrovirus
    Jonathan E Breitenbach
    Biological Control of Insects Research Laboratory, USDA Agricultural Research Service, Columbia, MO, USA
    Virus Res 171:194-208. 2013
    ....
  14. doi request reprint Genetic variation and virulence of nucleopolyhedroviruses isolated worldwide from the heliothine pests Helicoverpa armigera, Helicoverpa zea, and Heliothis virescens
    Daniel L Rowley
    Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, USDA Agricultural Research Service, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    J Invertebr Pathol 107:112-26. 2011
    ....
  15. ncbi request reprint Transforming lepidopteran insect cells for improved protein processing
    Robert L Harrison
    USDA ARS Insect Biocontrol Laboratory, Beltsville, MD, USA
    Methods Mol Biol 388:341-56. 2007
    ..Examples of lepidopteran insect cell lines engineered for improved protein N-glycosylation and trafficking are described...
  16. ncbi request reprint Transforming lepidopteran insect cells for continuous recombinant protein expression
    Robert L Harrison
    USDA ARS Insect Biocontrol Laboratory, Beltsville, MD, USA
    Methods Mol Biol 388:299-316. 2007
    ..Techniques for the insertion of genes into continuous expression vectors, transfection of cells, and the selection and isolation of stably transformed Sf-9 clones by either colony formation or end-point dilution are described in detail...
  17. doi request reprint Autographa californica multiple nucleopolyhedrovirus ODV-E56 is a per os infectivity factor, but is not essential for binding and fusion of occlusion-derived virus to the host midgut
    Wendy O Sparks
    Genetics Program, Department of Entomology, Iowa State University, Ames, IA 50011, USA
    Virology 409:69-76. 2011
    ..These results indicate that ODV-E56 is a per os infectivity factor (pif-5) required for primary midgut infection at a point before or after virion binding and fusion...
  18. ncbi request reprint Protein N-glycosylation in the baculovirus-insect cell expression system and engineering of insect cells to produce "mammalianized" recombinant glycoproteins
    Robert L Harrison
    Insect Biocontrol Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
    Adv Virus Res 68:159-91. 2006
    ..In addition, we consider other possible strategies for improving glycosylation in insect cells...
  19. ncbi request reprint Impact of a basement membrane-degrading protease on dissemination and secondary infection of Autographa californica multiple nucleopolyhedrovirus in Heliothis virescens (Fabricus)
    Huarong Li
    Department of Entomology, Iowa State University, Ames, IA 50011, USA
    J Gen Virol 88:1109-19. 2007
    ..Implications for the role of the BM as a barrier to baculovirus dissemination within the host insect are discussed...
  20. doi request reprint Insecticidal activity of a basement membrane-degrading protease against Heliothis virescens (Fabricius) and Acyrthosiphon pisum (Harris)
    Huarong Li
    Department of Entomology, Iowa State University, 418 Science II, Ames, IA 50011 3222, USA
    J Insect Physiol 54:777-89. 2008
    ..These results illustrate the potent insecticidal effects of ScathL cysteine protease activity and the potential for use of ScathL in development of insect resistant transgenic plants when combined with an appropriate delivery system...
  21. ncbi request reprint Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses
    Robert L Harrison
    Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
    J Gen Virol 84:1827-42. 2003
    ..Maximum-likelihood analysis of selection pressures on AcMNPV and RoMNPV ORFs indicate that ORFs ro18/ac20-ac21 (arif-1) and ro135/ac143 (odv-e18) have undergone positive selection...
  22. ncbi request reprint Characterisation of functional and insecticidal properties of a recombinant cathepsin L-like proteinase from flesh fly (Sarcophaga peregrina), which plays a role in differentiation of imaginal discs
    Judith M D Philip
    School of Biological and Biomedical Sciences, Durham University, South Road, Durham, UK
    Insect Biochem Mol Biol 37:589-600. 2007
    ..Production of a inactive mutant form of ScathL showed that enzyme activity was necessary for the complete proprotein processing observed during production as a recombinant protein, and for insecticidal activity...
  23. ncbi request reprint Tissue specificity of a baculovirus-expressed, basement membrane-degrading protease in larvae of Heliothis virescens
    Hailin Tang
    Department of Entomology, Iowa State University, 418 Science II, Ames, IA 50011 3222, USA
    Tissue Cell 39:431-43. 2007
    ..Insect Biochem. Mol. Biol. 37, 589-600) of the remarkable specificity of this protease...
  24. ncbi request reprint Application of maximum-likelihood models to selection pressure analysis of group I nucleopolyhedrovirus genes
    Robert L Harrison
    Department of Entomology and Interdepartmental Program in Genetics, Iowa State University, Ames, IA 50011, USA
    J Gen Virol 85:197-210. 2004
    ....