Clifton K Fagerquist

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. doi request reprint Possible evidence of amide bond formation between sinapinic acid and lysine-containing bacterial proteins by matrix-assisted laser desorption/ionization (MALDI) at 355 nm
    Clifton K Fagerquist
    Agricultural Research Service, US Department of Agriculture, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
    J Am Soc Mass Spectrom 23:2102-14. 2012
  2. doi request reprint A new calibrant for matrix-assisted laser desorption/ionization time-of-flight-time-of-flight post-source decay tandem mass spectrometry of non-digested proteins for top-down proteomic analysis
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA
    Rapid Commun Mass Spectrom 26:1241-8. 2012
  3. pmc Web-based software for rapid top-down proteomic identification of protein biomarkers, with implications for bacterial identification
    Clifton K Fagerquist
    Western Regional Research Center, U S Department of Agriculture, Albany, CA 94710, USA
    Appl Environ Microbiol 75:4341-53. 2009
  4. pmc Top-down proteomic identification of furin-cleaved α-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
    J Biomed Biotechnol 2010:123460. 2010
  5. doi request reprint Rapid identification of protein biomarkers of Escherichia coli O157:H7 by matrix-assisted laser desorption ionization-time-of-flight-time-of-flight mass spectrometry and top-down proteomics
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    Anal Chem 82:2717-25. 2010
  6. doi request reprint Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, California 94710, USA
    J Am Soc Mass Spectrom 21:819-32. 2010
  7. doi request reprint Induction and identification of disulfide-intact and disulfide-reduced β-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS and top-down proteomics
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
    Analyst 136:1739-46. 2011
  8. ncbi request reprint Sub-speciating Campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Proteome Res 5:2527-38. 2006
  9. pmc RcsB contributes to the distinct stress fitness among Escherichia coli O157:H7 curli variants of the 1993 hamburger-associated outbreak strains
    Michelle Q Carter
    Produce Safety and Microbiology Unit, Western Regional Research Center, Agricultural Research Service, USDA, Albany, California, USA
    Appl Environ Microbiol 78:7706-19. 2012
  10. ncbi request reprint Confirmatory and quantitative analysis of beta-lactam antibiotics in bovine kidney tissue by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, USA
    Anal Chem 77:1473-82. 2005

Collaborators

Detail Information

Publications22

  1. doi request reprint Possible evidence of amide bond formation between sinapinic acid and lysine-containing bacterial proteins by matrix-assisted laser desorption/ionization (MALDI) at 355 nm
    Clifton K Fagerquist
    Agricultural Research Service, US Department of Agriculture, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
    J Am Soc Mass Spectrom 23:2102-14. 2012
    ....
  2. doi request reprint A new calibrant for matrix-assisted laser desorption/ionization time-of-flight-time-of-flight post-source decay tandem mass spectrometry of non-digested proteins for top-down proteomic analysis
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA
    Rapid Commun Mass Spectrom 26:1241-8. 2012
    ..However, there is no commonly accepted calibrant for this purpose beyond the use of peptide calibrants whose fragment ions span a lower mass-to-charge (m/z) range...
  3. pmc Web-based software for rapid top-down proteomic identification of protein biomarkers, with implications for bacterial identification
    Clifton K Fagerquist
    Western Regional Research Center, U S Department of Agriculture, Albany, CA 94710, USA
    Appl Environ Microbiol 75:4341-53. 2009
    ....
  4. pmc Top-down proteomic identification of furin-cleaved α-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
    J Biomed Biotechnol 2010:123460. 2010
    ..The peak at m/z 5286 was definitively identified by MALDI-TOF-TOF-MS/MS and top-down proteomics as the A2 fragment of α-Stx2...
  5. doi request reprint Rapid identification of protein biomarkers of Escherichia coli O157:H7 by matrix-assisted laser desorption ionization-time-of-flight-time-of-flight mass spectrometry and top-down proteomics
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    Anal Chem 82:2717-25. 2010
    ..coli O157:H7 strains was identified by top-down analysis to be the HdeB acid stress chaperone-like protein consistent with previous identifications by gene sequencing and bottom-up proteomics...
  6. doi request reprint Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, California 94710, USA
    J Am Soc Mass Spectrom 21:819-32. 2010
    ....
  7. doi request reprint Induction and identification of disulfide-intact and disulfide-reduced β-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS and top-down proteomics
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
    Analyst 136:1739-46. 2011
    ..Easier to interpret MS/MS data were obtained for the disulfide-reduced β-Stx2 which resulted in an improved top-down identification...
  8. ncbi request reprint Sub-speciating Campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Proteome Res 5:2527-38. 2006
    ....
  9. pmc RcsB contributes to the distinct stress fitness among Escherichia coli O157:H7 curli variants of the 1993 hamburger-associated outbreak strains
    Michelle Q Carter
    Produce Safety and Microbiology Unit, Western Regional Research Center, Agricultural Research Service, USDA, Albany, California, USA
    Appl Environ Microbiol 78:7706-19. 2012
    ....
  10. ncbi request reprint Confirmatory and quantitative analysis of beta-lactam antibiotics in bovine kidney tissue by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, USA
    Anal Chem 77:1473-82. 2005
    ....
  11. pmc Evolutionary silence of the acid chaperone protein HdeB in enterohemorrhagic Escherichia coli O157:H7
    Michelle Q Carter
    Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, California, USA
    Appl Environ Microbiol 78:1004-14. 2012
    ..These results suggest a divergent evolution of acid resistance mechanisms within E. coli...
  12. ncbi request reprint Genomic and proteomic identification of a DNA-binding protein used in the "fingerprinting" of campylobacter species and strains by MALDI-TOF-MS protein biomarker analysis
    Clifton K Fagerquist
    Western Regional Research Service, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, USA
    Anal Chem 77:4897-907. 2005
    ..upsaliensis strains. We have also tentatively assigned a approximately 10.5-kDa protein biomarker of a C. concisus strain as an HU protein...
  13. ncbi request reprint Composite sequence proteomic analysis of protein biomarkers of Campylobacter coli, C. lari and C. concisus for bacterial identification
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    Analyst 132:1010-23. 2007
    ..The sequence obtained could then be used as a strain-specific biomarker for analysis by 'top-down' proteomics techniques...
  14. ncbi request reprint Amino acid sequence determination of protein biomarkers of Campylobacter upsaliensis and C. helveticus by "composite" sequence proteomic analysis
    Clifton K Fagerquist
    Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Proteome Res 6:2539-49. 2007
    ....
  15. ncbi request reprint Quantification and characterization of mucosa-associated and intracellular Escherichia coli in inflammatory bowel disease
    Timothy R Elliott
    Diabetes and Nutritional Sciences Division, King s College London, London, United Kingdom Department of Gastroenterology, Guy s and St Thomas NHS Foundation Trust, St Thomas Hospital, London, United Kingdom Department of Medicine, University of Melbourne, Melbourne, Australia Animal Health and Veterinary Laboratories Agency, Surrey, United Kingdom and US Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Produce Safety and Microbiology Research Unit, Albany, California
    Inflamm Bowel Dis 19:2326-38. 2013
    ..This study aimed to quantify and characterize mucosa-associated and intracellular E. coli in patients with IBD and in healthy control subjects (HC)...
  16. pmc Speciation of Campylobacter coli, C. jejuni, C. helveticus, C. lari, C. sputorum, and C. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Robert E Mandrell
    USDA, ARS, WRRC, 800 Buchanan St, Albany, CA 94710, USA
    Appl Environ Microbiol 71:6292-307. 2005
    ..MALDI-TOF MS provides an accurate, sensitive, and rapid method for identification of multiple Campylobacter species relevant to public health and food safety...
  17. ncbi request reprint Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization-Tandem Time of Flight Mass Spectrometry
    Clifton K Fagerquist
    Produce Safety Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Albany, California, USA
    Appl Environ Microbiol 80:2928-40. 2014
    ..Top-down results were also compared to a bioassay using a Vero-d2EGFP cell line. Our results suggest that top-down proteomic identification is a rapid, highly specific technique for distinguishing Stx2 subtypes. ..
  18. ncbi request reprint Collision-activated cleavage of a peptide/antibiotic disulfide linkage: possible evidence for intramolecular disulfide bond rearrangement upon collisional activation
    Clifton K Fagerquist
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, PA, USA
    Rapid Commun Mass Spectrom 18:685-700. 2004
    ..These results demonstrate the facile dissociative loss by CAD of DFC moieties covalently attached to peptides through disulfide bonds. Published in 2004 by John Wiley & Sons, Ltd...
  19. ncbi request reprint Identification of (12-ricinoleoylricinoleoyl)diricinoleoylglycerol, an acylglycerol containing four acyl chains, in castor (Ricinus communis L.) oil by LC-ESI-MS
    Jiann Tsyh Lin
    Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
    J Agric Food Chem 54:3498-504. 2006
    ..This newly identified and biosynthesized RRRR represents a new AG subclass of tetra-acylglycerols (or acylacyldiacylglycerol)...
  20. ncbi request reprint An antibiotic linked to peptides and proteins is released by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry
    Clifton K Fagerquist
    United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA
    J Am Soc Mass Spectrom 14:302-10. 2003
    ..ECD of [insulin + DFC + 4H](4+) shows preferential dissociative loss of DFC. Combined with HPLC, ECD FT-ICR-MS may be an efficient screening method for detection of drug-biomolecule binding...
  21. ncbi request reprint Confirmatory analysis of beta-lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometry
    Clifton K Fagerquist
    Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
    Rapid Commun Mass Spectrom 17:660-71. 2003
    ..We also found that SRM MS(n) was superior in the analysis of unknown incurred tissue than full spectrum MS(n). We also obtained an MS/MS spectrum of DCCD that is significantly at variance with previously published fragmentation spectra...
  22. ncbi request reprint Implication of C-terminal deletion on the structure and stability of bovine beta-casein
    Phoebe X Qi
    U S Department of Agriculture, Eastern Regional Research Center, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA, 19038, USA
    Protein J 24:431-44. 2005
    ..It has been demonstrated clearly that the tail peptide beta-CN-(f193-209) is important in maintaining the hydrophobic core of beta-CN but the residual association observed argues for a minor role for other sites as well...