Research Topics
| Clifton K FagerquistSummaryAffiliation: Agricultural Research Service Country: USA Publications
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Detail Information
Publications
Possible evidence of amide bond formation between sinapinic acid and lysine-containing bacterial proteins by matrix-assisted laser desorption/ionization (MALDI) at 355 nmClifton K Fagerquist
Agricultural Research Service, US Department of Agriculture, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
J Am Soc Mass Spectrom 23:2102-14. 2012....
A new calibrant for matrix-assisted laser desorption/ionization time-of-flight-time-of-flight post-source decay tandem mass spectrometry of non-digested proteins for top-down proteomic analysisClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA
Rapid Commun Mass Spectrom 26:1241-8. 2012..However, there is no commonly accepted calibrant for this purpose beyond the use of peptide calibrants whose fragment ions span a lower mass-to-charge (m/z) range...
Top-down proteomic identification of furin-cleaved α-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MSClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
J Biomed Biotechnol 2010:123460. 2010..The peak at m/z 5286 was definitively identified by MALDI-TOF-TOF-MS/MS and top-down proteomics as the A2 fragment of α-Stx2...
Rapid identification of protein biomarkers of Escherichia coli O157:H7 by matrix-assisted laser desorption ionization-time-of-flight-time-of-flight mass spectrometry and top-down proteomicsClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
Anal Chem 82:2717-25. 2010..coli O157:H7 strains was identified by top-down analysis to be the HdeB acid stress chaperone-like protein consistent with previous identifications by gene sequencing and bottom-up proteomics...
Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometryClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, California 94710, USA
J Am Soc Mass Spectrom 21:819-32. 2010....
Web-based software for rapid top-down proteomic identification of protein biomarkers, with implications for bacterial identificationClifton K Fagerquist
Western Regional Research Center, U S Department of Agriculture, Albany, CA 94710, USA
Appl Environ Microbiol 75:4341-53. 2009....
Induction and identification of disulfide-intact and disulfide-reduced β-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS and top-down proteomicsClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
Analyst 136:1739-46. 2011..Easier to interpret MS/MS data were obtained for the disulfide-reduced β-Stx2 which resulted in an improved top-down identification...
Sub-speciating Campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modificationsClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
J Proteome Res 5:2527-38. 2006....
RcsB contributes to the distinct stress fitness among Escherichia coli O157:H7 curli variants of the 1993 hamburger-associated outbreak strainsMichelle Q Carter
Produce Safety and Microbiology Unit, Western Regional Research Center, Agricultural Research Service, USDA, Albany, California, USA
Appl Environ Microbiol 78:7706-19. 2012....
Confirmatory and quantitative analysis of beta-lactam antibiotics in bovine kidney tissue by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometryClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, USA
Anal Chem 77:1473-82. 2005....
Evolutionary silence of the acid chaperone protein HdeB in enterohemorrhagic Escherichia coli O157:H7Michelle Q Carter
Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, California, USA
Appl Environ Microbiol 78:1004-14. 2012..These results suggest a divergent evolution of acid resistance mechanisms within E. coli...
Genomic and proteomic identification of a DNA-binding protein used in the "fingerprinting" of campylobacter species and strains by MALDI-TOF-MS protein biomarker analysisClifton K Fagerquist
Western Regional Research Service, Agricultural Research Service, U S Department of Agriculture, Albany, California 94710, USA
Anal Chem 77:4897-907. 2005..upsaliensis strains. We have also tentatively assigned a approximately 10.5-kDa protein biomarker of a C. concisus strain as an HU protein...
Composite sequence proteomic analysis of protein biomarkers of Campylobacter coli, C. lari and C. concisus for bacterial identificationClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
Analyst 132:1010-23. 2007..The sequence obtained could then be used as a strain-specific biomarker for analysis by 'top-down' proteomics techniques...
Amino acid sequence determination of protein biomarkers of Campylobacter upsaliensis and C. helveticus by "composite" sequence proteomic analysisClifton K Fagerquist
Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
J Proteome Res 6:2539-49. 2007....
Speciation of Campylobacter coli, C. jejuni, C. helveticus, C. lari, C. sputorum, and C. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometryRobert E Mandrell
USDA, ARS, WRRC, 800 Buchanan St, Albany, CA 94710, USA
Appl Environ Microbiol 71:6292-307. 2005..MALDI-TOF MS provides an accurate, sensitive, and rapid method for identification of multiple Campylobacter species relevant to public health and food safety...
Collision-activated cleavage of a peptide/antibiotic disulfide linkage: possible evidence for intramolecular disulfide bond rearrangement upon collisional activationClifton K Fagerquist
Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, PA, USA
Rapid Commun Mass Spectrom 18:685-700. 2004..These results demonstrate the facile dissociative loss by CAD of DFC moieties covalently attached to peptides through disulfide bonds. Published in 2004 by John Wiley & Sons, Ltd...
Identification of (12-ricinoleoylricinoleoyl)diricinoleoylglycerol, an acylglycerol containing four acyl chains, in castor (Ricinus communis L.) oil by LC-ESI-MSJiann Tsyh Lin
Western Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA
J Agric Food Chem 54:3498-504. 2006..This newly identified and biosynthesized RRRR represents a new AG subclass of tetra-acylglycerols (or acylacyldiacylglycerol)...
An antibiotic linked to peptides and proteins is released by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometryClifton K Fagerquist
United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, Pennsylvania 19038, USA
J Am Soc Mass Spectrom 14:302-10. 2003..ECD of [insulin + DFC + 4H](4+) shows preferential dissociative loss of DFC. Combined with HPLC, ECD FT-ICR-MS may be an efficient screening method for detection of drug-biomolecule binding...
Confirmatory analysis of beta-lactam antibiotics in kidney tissue by liquid chromatography/electrospray ionization selective reaction monitoring ion trap tandem mass spectrometryClifton K Fagerquist
Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
Rapid Commun Mass Spectrom 17:660-71. 2003..We also found that SRM MS(n) was superior in the analysis of unknown incurred tissue than full spectrum MS(n). We also obtained an MS/MS spectrum of DCCD that is significantly at variance with previously published fragmentation spectra...
Implication of C-terminal deletion on the structure and stability of bovine beta-caseinPhoebe X Qi
U S Department of Agriculture, Eastern Regional Research Center, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA, 19038, USA
Protein J 24:431-44. 2005..It has been demonstrated clearly that the tail peptide beta-CN-(f193-209) is important in maintaining the hydrophobic core of beta-CN but the residual association observed argues for a minor role for other sites as well...
