Jitender P Dubey

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. doi request reprint Seroprevalence and isolation of Toxoplasma gondii from free-range chickens in Ghana, Indonesia, Italy, Poland, and Vietnam
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 94:68-71. 2008
  2. doi request reprint Isolation of viable Neospora caninum from brains of wild gray wolves (Canis lupus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 201:150-3. 2014
  3. doi request reprint Genotyping Toxoplasma gondii from wildlife in Pennsylvania and identification of natural recombinants virulent to mice
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 200:74-84. 2014
  4. doi request reprint Molecular and biological characterization of first isolates of Hammondia hammondi from cats from Ethiopia
    J P Dubey
    US Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
    J Parasitol 99:614-8. 2013
  5. doi request reprint Isolation and RFLP genotyping of Toxoplasma gondii from the gray wolf (Canis lupus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 197:685-90. 2013
  6. doi request reprint A new atypical genotype mouse virulent strain of Toxoplasma gondii isolated from the heart of a wild caught puma (Felis concolor) from Durango, Mexico
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 197:674-7. 2013
  7. doi request reprint Isolation and RFLP genotyping of Toxoplasma gondii from the domestic dogs (Canis familiaris) from Grenada, West Indies revealed high genetic variability
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 197:623-6. 2013
  8. doi request reprint Seropositivity of Toxoplasma gondii in domestic donkeys (Equus asinus) and isolation of T. gondii from farm cats
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705, USA Electronic address
    Vet Parasitol 199:18-23. 2014
  9. pmc Prevalence of antibodies to Leishmania infantum and Toxoplasma gondii in horses from the north of Portugal
    Ana Patrícia Lopes
    Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás os Montes e Alto Douro UTAD, Vila Real, Portugal
    Parasit Vectors 6:178. 2013
  10. pmc Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
    Alessia Possenti
    Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome 00161, Italy
    BMC Genomics 14:183. 2013

Collaborators

Detail Information

Publications152 found, 100 shown here

  1. doi request reprint Seroprevalence and isolation of Toxoplasma gondii from free-range chickens in Ghana, Indonesia, Italy, Poland, and Vietnam
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 94:68-71. 2008
    ..This is the first report of genetic characterization of T. gondii isolates from animals from these countries...
  2. doi request reprint Isolation of viable Neospora caninum from brains of wild gray wolves (Canis lupus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 201:150-3. 2014
    ..caninum tachyzoites of the two isolates were analyzed by N. caninum-specific Nc5 polymerase chain reaction and confirmed diagnosis. This is the first report of isolation of N. caninum from tissues of any wild canid host. ..
  3. doi request reprint Genotyping Toxoplasma gondii from wildlife in Pennsylvania and identification of natural recombinants virulent to mice
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 200:74-84. 2014
    ..The results showed that there is an association of genotypes of ROP5 and ROP18 with mouse-virulence, however, additional gene(s) may also contribute to virulence in distinct T. gondii genotypes. ..
  4. doi request reprint Molecular and biological characterization of first isolates of Hammondia hammondi from cats from Ethiopia
    J P Dubey
    US Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
    J Parasitol 99:614-8. 2013
    ..hammondi-specific primers, and sequences were deposited in GenBank (accession nos. JX477424, and KC223619). This is the first report of isolation of H. hammondi from cats from the African continent...
  5. doi request reprint Isolation and RFLP genotyping of Toxoplasma gondii from the gray wolf (Canis lupus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 197:685-90. 2013
    ..Four genotypes were detected. Twenty-one isolates were Type 12 (ToxoDB PCR-RFLP genotype #5), 2 were Type II clonal (ToxoDB #1), 1 was Type II variant (ToxoDB #3), and 1 was a new genotype designated as ToxoDB genotype #219...
  6. doi request reprint A new atypical genotype mouse virulent strain of Toxoplasma gondii isolated from the heart of a wild caught puma (Felis concolor) from Durango, Mexico
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 197:674-7. 2013
    ..Isolation of atypical genotype T. gondii from wild puma indicates that mouse virulent strains are circulating in wildlife in Mexico...
  7. doi request reprint Isolation and RFLP genotyping of Toxoplasma gondii from the domestic dogs (Canis familiaris) from Grenada, West Indies revealed high genetic variability
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA Electronic address
    Vet Parasitol 197:623-6. 2013
    ..The result supports previous findings that T. gondii population genetics is highly diverse in Grenada...
  8. doi request reprint Seropositivity of Toxoplasma gondii in domestic donkeys (Equus asinus) and isolation of T. gondii from farm cats
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705, USA Electronic address
    Vet Parasitol 199:18-23. 2014
    ..The role of barn cats in the transmission of T. gondii to donkeys on farms warrents further investigation...
  9. pmc Prevalence of antibodies to Leishmania infantum and Toxoplasma gondii in horses from the north of Portugal
    Ana Patrícia Lopes
    Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás os Montes e Alto Douro UTAD, Vila Real, Portugal
    Parasit Vectors 6:178. 2013
    ..Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal...
  10. pmc Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle
    Alessia Possenti
    Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome 00161, Italy
    BMC Genomics 14:183. 2013
    ..The oocyst plays a central epidemiological role, yet this stage has been scarcely investigated at the molecular level and the knowledge of its expressed proteome is very limited...
  11. doi request reprint Congenital transmission of Neospora caninum in white-tailed deer (Odocoileus virginianus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
    Vet Parasitol 196:519-22. 2013
    ..DNA obtained from culture-derived N. caninum tachyzoites of the two isolates with Nc5 PCR confirmed diagnosis. Results prove congenital transmission of N. caninum in the white tailed deer for the first time. ..
  12. doi request reprint Sarcocystis neurona infection in gamma interferon gene knockout (KO) mice: comparative infectivity of sporocysts in two strains of KO mice, effect of trypsin digestion on merozoite viability, and infectivity of bradyzoites to KO mice and cell culture
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 207 05 2350, USA
    Vet Parasitol 196:212-5. 2013
    ..S. neurona merozoites survived 120 min incubation in 0.25% trypsin, indicating that trypsin digestion can be used to recover S. neurona from tissues of acutely infected animals...
  13. doi request reprint Genetic diversity of Toxoplasma gondii isolates from Ethiopian feral cats
    J P Dubey
    United States of Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 196:206-8. 2013
    ..gondii infection. These findings are of epidemiological significance with respect to shedding of oocysts by cats. This is the first report of genotyping of T. gondii from any host in Ethiopia. ..
  14. doi request reprint Isolation and characterization of new genetic types of Toxoplasma gondii and prevalence of Trichinella murrelli from black bear (Ursus americanus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
    Vet Parasitol 196:24-30. 2013
    ..Two bears were infected with Trichinella spp.; one in 2008 and one in 2009. Genotyping of collected muscle larvae revealed that the infecting species in both cases was Trichinella murrelli...
  15. doi request reprint Development of early tissue cysts and associated pathology of Besnoitia besnoiti in a naturally infected bull (Bos taurus) from South Africa
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, BARC East, Beltsville, Maryland 20705 2350, USA
    J Parasitol 99:459-66. 2013
    ..Associated clinical findings, lesions, and histopathology are briefly presented. The bull died of nephrotic syndrome; anasarca in acute besnoitiosis due to protein-losing glomerulopathy is a finding not previously reported in cattle...
  16. doi request reprint Isolation and genetic characterization of Toxoplasma gondii from mute swan (Cygnus olor) from the USA
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 195:42-6. 2013
    ..Isolation of T. gondii from mute swan indicates that the local waters were contaminated by T. gondii oocysts, and that mouse T. gondii virulent strains are circulating in wildlife. Mute swan is a new host record for T. gondii...
  17. doi request reprint Isolation of viable Toxoplasma gondii from tissues and feces of cats from Addis Ababa, Ethiopia
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 99:56-8. 2013
    ..The high prevalence of T. gondii oocysts in feces of 8 (19.4%) of 36 cats is of high epidemiologic significance. This is the first report of isolation of viable T. gondii from any host in Ethiopia...
  18. doi request reprint Ante-mortem diagnosis, diarrhea, oocyst shedding, treatment, isolation, and genetic typing of Toxoplasma gondii associated with clinical toxoplasmosis in a naturally infected cat
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 99:158-60. 2013
    ..4, which is grouped into the Type 12 lineage that is dominant in wildlife from North America. To our knowledge, this is the first T. gondii isolate characterized genetically from a sick cat in the USA...
  19. doi request reprint High prevalence of Toxoplasma gondii antibodies in domestic pigs in Oaxaca State, Mexico
    C Alvarado-Esquivel
    Faculty of Medicine and Nutrition, Juárez University of Durango State Avenida Universidad S N 34000 Durango, Mexico
    J Parasitol 98:1248-50. 2012
    ..Because there is no national system of determining the T. gondii infection status at the time of slaughter, precautions should be taken while handling pig carcasses, and all pork should be cooked thoroughly before human consumption...
  20. doi request reprint Sarcocystis neurona retinochoroiditis in a sea otter (Enhydra lutris kenyoni)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 183:156-9. 2011
    ..Immunohistochemistry with S. neurona-specific polyclonal rabbit antibodies stained schizonts and merozoites. To our knowledge this is the first report of S. neurona-associated retinochoroiditis in any naturally infected animal...
  21. doi request reprint Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiology
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, BARC East, Beltsville, MD 20705 2350, USA
    Parasitology 139:1375-424. 2012
    ..The severity of clinical toxoplasmosis in Brazilian children may be associated with the genetic characteristics of T. gondii isolates prevailing in animals and humans in Brazil...
  22. ncbi request reprint A review of toxoplasmosis in humans and animals in Ethiopia
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705, USA
    Epidemiol Infect 140:1935-8. 2012
    ..Serological surveys indicate up to 79% of goats and sheep have T. gondii antibodies. However, there is no information on losses due to toxoplasmosis in livestock or the presence of viable T. gondii in any host in Ethiopia...
  23. doi request reprint Seroprevalence of Toxoplasma gondii infection in domestic horses in Durango State, Mexico
    C Alvarado-Esquivel
    Juárez University of Durango State, Avenida Universidad s n, 34000 Durango, Mexico
    J Parasitol 98:944-5. 2012
    ..5%) than in pastured horses (3.1%). Results indicate that infected horses represent a potential source of T. gondii infection for humans in Mexico. This is the first report of T. gondii infection in horses in Mexico...
  24. doi request reprint High prevalence and genotypes of Toxoplasma gondii isolated from organic pigs in northern USA
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 188:14-8. 2012
    ..These results revealed very high prevalence of T. gondii in organic pigs for the first time in USA, indicating potentially increased health risk of consuming organic swine products...
  25. pmc Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out-bred, in-bred) on pathogenesis and mortality
    J P Dubey
    United States Department of Agriculture, Animal Natural Resources Institute, Animal Parasitic Disease Laboratory, BARC East, Beltsville, MD 20705 2350, USA
    Parasitology 139:1-13. 2012
    ..gondii using the same preparations of oocysts which are stable for up to a year, and to have highly reproducible responses to the infection...
  26. pmc Whole genome sequencing of a natural recombinant Toxoplasma gondii strain reveals chromosome sorting and local allelic variants
    Irene Lindström Bontell
    Institute of Integrative and Comparative Biology, Clarendon Way, University of Leeds, Leeds LS2 9JT, UK
    Genome Biol 10:R53. 2009
    ..Little is known of the genotypes present in Africa despite the importance of AIDS-associated toxoplasmosis...
  27. doi request reprint Seroprevalence of Toxoplasma gondii and Bartonella spp. antibodies in cats from Pennsylvania
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 95:578-80. 2009
    ..gondii and Bartonella spp. seropositivity. Overall the seroprevalence of T. gondii antibodies in cats in this population is much lower compared with those reported in other localities in the United States and other countries...
  28. doi request reprint Fatal intestinal coccidiosis in a three week old buffalo calf (Bubalus bubalus)
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Beltsville, United States Department of Agriculture, Maryland, USA
    J Parasitol 94:1289-94. 2008
    ..No coccidian stages were seen in sections of colon. This is one of the first confirmed cases of clinical coccidiosis in water buffalo...
  29. doi request reprint Toxoplasmosis in captive dolphins (Tursiops truncatus) and walrus (Odobenus rosmarus)
    J P Dubey
    Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705 2350, USA
    J Parasitol 95:82-5. 2009
    ..1 and the walrus showed archetypal alleles consistent with infection by a Type II strain. No unique polymorphisms were detected. This is apparently the first report of isolation of T. gondii from a marine mammal in Canada...
  30. doi request reprint Endemic toxoplasmosis in pigs on a farm in Maryland: isolation and genetic characterization of Toxoplasma gondii
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 94:36-41. 2008
    ..Both genotypes 1 and 2 are considered to belong to the clonal type II lineages. Genotype 3 and 4 are nonclonal isolates. Results document high prevalence of T. gondii in pigs on a farm in Maryland...
  31. doi request reprint Toxoplasma gondii and Neospora caninum antibodies in dogs from Grenada, West Indies
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
    J Parasitol 94:750-1. 2008
    ..Two of the 107 dogs had N. caninum antibodies (IFAT titers 1:100 and 1:400); these dogs had T. gondii titers of 1:1,600 and 1:50, respectively. Results indicate that these 2 structurally similar protozoa are antigenically different...
  32. doi request reprint Seroprevalence of Neospora caninum and Toxoplasma gondii in black-tailed deer (Odocoileus hemionus columbianus) and mule deer (Odocoileus hemionus hemionus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 156:310-3. 2008
    ..T. gondii antibodies were found in 14 black-tailed deer but not in any of the mule deer. This is probably the first report of seroprevalence of N. caninum in these hosts...
  33. doi request reprint Genetic diversity of Toxoplasma gondii isolates from chickens from Brazil
    J P Dubey
    United States Department of Agriculture, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
    Vet Parasitol 157:299-305. 2008
    ..Two isolates had mixed infections. Clonal Type II strain was absent. One strain was Type II at all loci, except BTUB. The results confirm high genetic diversity of T. gondii isolates from Brazil...
  34. doi request reprint Isolation of Toxoplasma gondii from the keel-billed toucan (Ramphastos sulfuratus) from Costa Rica
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 95:467-8. 2009
    ..The isolate designated TgRsCrl was found to be non-clonal with Type I, II, and III alleles at different loci. This is the first isolation of T. gondii from this host...
  35. doi request reprint The history of Toxoplasma gondii--the first 100 years
    Jitender P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Eukaryot Microbiol 55:467-75. 2008
    ..gondii oocysts washed from land. Hygiene remains the best preventive measure because currently there is no vaccine to prevent toxoplasmosis in humans...
  36. doi request reprint Isolation of Toxoplasma gondii from animals in Durango, Mexico
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705 2350, USA
    J Parasitol 95:319-22. 2009
    ..One genotype (the 4 chicken isolates) belongs to the clonal Type III lineage, three genotypes were reported in previous reports, and 1 genotype is unique...
  37. doi request reprint Toxoplasmosis in wallabies (Macropus rufogriseus and Macropus eugenii): blindness, treatment with atovaquone, and isolation of Toxoplasma gondii
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
    J Parasitol 94:929-33. 2008
    ..gondii from wallabies in the United States...
  38. doi request reprint Isolation of Toxoplasma gondii from bottlenose dolphins (Tursiops truncatus)
    J P Dubey
    Animal Parasitic Deseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705, USA
    J Parasitol 94:821-3. 2008
    ..Two of the 3 isolates have Type II alleles at all loci and belong to the clonal Type II lineage. One isolate has a unique genotype. This is the first report of isolation of viable T. gondii from T. truncatus...
  39. doi request reprint Mouse-virulent Toxoplasma gondii isolated from feral cats on Mona Island, Puerto Rico
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705, USA
    J Parasitol 93:1365-9. 2007
    ..The latter isolates of T. gondii were different biologically and phenotypically from the feline isolates from the rest of the Americas. One isolate (TgCatPr 12) was a mixed infection with 2 genotypes...
  40. doi request reprint Toxoplasma gondii infection in Blanford's fox (Vulpes cana)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 153:147-51. 2008
    ..Protozoal tachyzoites reacted positively with T. gondii-specific polyclonal antibodies. Antibodies to T. gondii were detected in 10 of 12 V. cana assayed by the latex agglutination or the modified direct agglutination test...
  41. doi request reprint High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USA
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
    Int J Parasitol 38:999-1006. 2008
    ..gondii in lambs, which has important implications in public health. We believe this is the first in-depth genetic analysis of T. gondii isolates from sheep in the USA...
  42. doi request reprint Transplacental toxoplasmosis in naturally-infected white-tailed deer: Isolation and genetic characterisation of Toxoplasma gondii from foetuses of different gestational ages
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
    Int J Parasitol 38:1057-63. 2008
    ..The most common genotype was Type II, commonly found in humans in North America and Europe, suggesting the possible link of transmission from game animals to humans...
  43. doi request reprint Neospora caninum and Toxoplasma gondii antibodies in dogs from Durango City, Mexico
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
    J Parasitol 93:1033-5. 2007
    ..The N. caninum antibody titers of the 2 dogs were: 1:400 by IFAT and 1:200 by NAT in 1, and 1:25 by NAT and IFAT in the other. Results indicate that these 2 structurally similar protozoans are antigenically different...
  44. doi request reprint Isolation and genetic characterization of Toxoplasma gondii from raccoons (Procyon lotor), cats (Felis domesticus), striped skunk (Mephitis mephitis), black bear (Ursus americanus), and cougar (Puma concolor) from Canada
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 94:42-5. 2008
    ..These results are in contrast with the Type II genotype that is widespread in domestic animals and humans throughout the United States and Europe. This is the first genotyping of T. gondii isolates from this part of North America...
  45. doi request reprint Toxoplasma gondii infection in humans and animals in the United States
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
    Int J Parasitol 38:1257-78. 2008
    ..Seroprevalence of T. gondii in humans and pigs is declining. Modes of transmission, epidemiology and environmental contamination with oocysts on land and sea are discussed...
  46. ncbi request reprint Neosporosis in Beagle dogs: clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninum
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agricultural, Building 1001, Beltsville, MD 20705, USA
    Vet Parasitol 149:158-66. 2007
    ..caninum antibody titers were still persistent in these pups at 23 months of age. Results indicate that medication with Clindamycin can improve clinical condition but not eliminate N. caninum infection...
  47. doi request reprint A new species of Sarcocystis (Apicomplexa: Sarcocystidae) from the black bear (Ursus americanus)
    J P Dubey
    Animal Parasitic Diseases Laboratory, USDA, Agricultural Research Service, Animal and Natural Resources Institute, BARC East, Building 1001, 10300 Baltimore Avenue, Beltsville, Maryland 20705 2350, USA
    J Parasitol 94:496-9. 2008
    ..0 microm long. The sarcocyst from the third bear was structurally different from S. ursusi; its sarcocyst wall was approximately 2 microm thick and had finger-like villi on the cyst wall giving the sarcocyst wall a striated appearance...
  48. doi request reprint Characterization of Toxoplasma gondii from raccoons (Procyon lotor), coyotes (Canis latrans), and striped skunks (Mephitis mephitis) in Wisconsin identified several atypical genotypes
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705, USA
    J Parasitol 93:1524-7. 2007
    ..The results indicate the prevalence of T. gondii in wildlife mammals is high and that these animals may serve as an important reservoir for transmission of T. gondii...
  49. doi request reprint The evolution of the knowledge of cat and dog coccidia
    J P Dubey
    Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705 2350, USA
    Parasitology 136:1469-75. 2009
    ..In the present paper I review salient features of the evolution of cat and dog coccidia...
  50. doi request reprint Seroprevalence of Toxoplasma gondii and concurrent Bartonella spp., feline immunodeficiency virus, and feline leukemia virus infections in cats from Grenada, West Indies
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, Maryland 20705 2350, USA
    J Parasitol 95:1129-33. 2009
    ..4% of 101 feral cats. Antibodies to FIV were found in 6 domestic and 22 feral cats. None of the 176 cats was positive for FeLv antigen. There was no correlation among T. gondii, Bartonella spp., and FIV seropositivity...
  51. doi request reprint Two new species of Sarcocystis (Apicomplexa: Sarcocystidae) infecting the wolverine (Gulo gulo) from Nunavut, Canada
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 96:972-6. 2010
    ..Sarcocystis kalvikus was found in 14 (34.1%), S. kitikmeotensis was found in 7 (17%), and both species were found in 12 (29.2%) of 41 wolverines...
  52. doi request reprint Isolation of viable Toxoplasma gondii from feral guinea fowl (Numida meleagris) and domestic rabbits (Oryctolagus cuniculus) from Brazil
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 97:842-5. 2011
    ..This is the first genetic characterization of T. gondii isolates from the rabbits and guinea fowl in Brazil and the first host record for T. gondii in the guinea fowl...
  53. doi request reprint Seroprevalence of Toxoplasma gondii infection in domestic pigs in Durango State, Mexico
    C Alvarado-Esquivel
    Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Durango State, Mexico
    J Parasitol 97:616-9. 2011
    ..gondii infection of pigs in Mexico and the first report on pigs from Durango State, Mexico. Results indicate that infected pork is likely an important source of T. gondii infection for humans in Durango State...
  54. doi request reprint Seroprevalence of Toxoplasma gondii and concurrent Bartonella spp., feline immunodeficiency virus, feline leukemia virus, and Dirofilaria immitis infections in Egyptian cats
    Y M Al-Kappany
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350
    J Parasitol 97:256-8. 2011
    ..4%) cats, respectively. The results indicate a high prevalence of T. gondii, Bartonella spp., and FIV infections in cats from Cairo, Egypt. This is the first report of Bartonella spp., and D. immitis infection in cats in Egypt...
  55. doi request reprint High prevalence and genotypes of Toxoplasma gondii isolated from goats, from a retail meat store, destined for human consumption in the USA
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Int J Parasitol 41:827-33. 2011
    ..gondii in goats, which have important implications in public health. We believe this is the first genetic analysis of T. gondii isolates from goats in the USA...
  56. doi request reprint Sporulation and survival of Toxoplasma gondii oocysts in different types of commercial cat litter
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 97:751-4. 2011
    ..Oocysts sporulated within 2-3 days in all types of cat litters and occasionally remained viable for 14 days. Results indicate that cat litter should be changed daily to prevent sporulation and infectivity to people...
  57. doi request reprint Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 181:382-7. 2011
    ..Tissue stages in mice stained positively with N. caninum-specific polyclonal antibodies. Our observation suggests that wolves may be an important link in the sylvatic cycle of N. caninum...
  58. doi request reprint Neosporosis in animals--the last five years
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
    Vet Parasitol 180:90-108. 2011
    ..This review is focused on current status of neosporosis in animals based on papers published in the last five years. Worldwide seroprevalences are tabulated. Strategies for control and prevention are discussed...
  59. doi request reprint Genetic characterisation of Toxoplasma gondii in wildlife from North America revealed widespread and high prevalence of the fourth clonal type
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Int J Parasitol 41:1139-47. 2011
    ..No clonal Type I strain was identified among these wildlife isolates. These results suggest that T. gondii strains in wildlife from North America have limited diversity, with the occurrence of only a few major clonal types...
  60. doi request reprint Toxoplasmosis in Sand cats (Felis margarita) and other animals in the Breeding Centre for Endangered Arabian Wildlife in the United Arab Emirates and Al Wabra Wildlife Preservation, the State of Qatar
    J P Dubey
    U S Department of Agriculture, Animal Natural Resources Institute, Animal Parasitic Disease Laboratory, Beltsville, MD 20705 2350, USA
    Vet Parasitol 172:195-203. 2010
    ..The genotype from the cat from AWWP (3657) is a genetic Type II strain with a Type I allele at locus Apico. This is the first report of genetic characterization of T. gondii isolates from Middle East...
  61. doi request reprint Besnoitia neotomofelis n. sp. (Protozoa: Apicomplexa) from the southern plains woodrat ( Neotoma micropus)
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, BARC East, Beltsville, MD 20705 2350, USA
    Parasitology 137:1731-47. 2010
    ..Besnoitia neotomofelis is distinct from the 3 other species of Besnoitia, B. wallacei, B. darlingi and B. oryctofelisi that utilize cats as a definitive host...
  62. doi request reprint Morphologic and molecular characterization of the sarcocysts of Sarcocystis rileyi (Apicomplexa: Sarcocystidae) from the mallard duck ( Anas platyrhynchos )
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    J Parasitol 96:765-70. 2010
    ....
  63. doi request reprint Toxoplasmosis in sheep--the last 20 years
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 163:1-14. 2009
    ..In the present paper, information on the prevalence, transmission, and control of ovine toxoplasmosis in the last 20 years is reviewed...
  64. doi request reprint Isolation and characterization of viable Toxoplasma gondii isolates revealed possible high frequency of mixed infection in feral cats ( Felis domesticus) from St Kitts, West Indies
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
    Parasitology 136:589-94. 2009
    ..Five of the 7 cats had infection with 2 genotypes, indicating high frequency of mixed infection in the cat population on the St Kitts island...
  65. ncbi request reprint Population biology of Toxoplasma gondii: what's out and where did they come from
    J P Dubey
    Animal Parasitic Diseases Laboratory, ANRI, ARS, USDA, Beltsville, Maryland 20705 2350, USA
    Mem Inst Oswaldo Cruz 104:190-5. 2009
    ..No clonal type II lineage was found. Of the 253 isolates from animals from USA, 18 genotypes were identified, predominantly type II. These studies indicate a higher genetic diversity than previously recognized...
  66. doi request reprint Toxoplasmosis in pigs--the last 20 years
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 164:89-103. 2009
    ..Recent studies on genetic typing of T. gondii strains prevalent in pigs are discussed with respect to epidemiology. Because wild pigs are hunted for food for human consumption prevalence in wild pigs is summarized...
  67. doi request reprint Serological and parasitological prevalence of Toxoplasma gondii in wild birds from Colorado
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
    J Parasitol 96:937-9. 2010
    ..This is the first time T. gondii has been isolated from the barn owl, ferruginous hawk, rough-legged hawk, and Swainson's hawk...
  68. ncbi request reprint History of the discovery of the life cycle of Toxoplasma gondii
    J P Dubey
    United States Department of Agriculture, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
    Int J Parasitol 39:877-82. 2009
    ..The discovery of the environmentally resistant stage of the parasite, the oocyst, made it possible to explain its worldwide prevalence. In the present paper, events associated with the discovery of its life cycle are recalled...
  69. doi request reprint Toxoplasma gondii infections in chickens (Gallus domesticus): prevalence, clinical disease, diagnosis and public health significance
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705 2350, USA
    Zoonoses Public Health 57:60-73. 2010
    ..A very high prevalence of the parasite was found in chickens raised in backyards (up to 100%) and free-range organic (30-50%) establishments...
  70. doi request reprint Toxoplasmosis and other intestinal coccidial infections in cats and dogs
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Clin North Am Small Anim Pract 39:1009-34, v. 2009
    ..Much remains to be learned concerning the pathogenesis of clinical coccidiosis...
  71. doi request reprint New Toxoplasma gondii genotypes isolated from free-range chickens from the Fernando de Noronha, Brazil: unexpected findings
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, MD 20705 2350, USA
    J Parasitol 96:709-12. 2010
    ..Results in this study indicate that T. gondii on this island consists of unique genotypes as well as clonal genotypes that are dominant in Europe and North America...
  72. doi request reprint A new atypical highly mouse virulent Toxoplasma gondii genotype isolated from a wild black bear in Alaska
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, MD 20705 2350, USA
    J Parasitol 96:713-6. 2010
    ..DNA sequencing at intron loci EF1, HP2, and UPRT1 revealed that the TgBbUS1 is a divergent T. gondii strain. These results indicate that mouse-virulent atypical T. gondii genotypes are also circulating in wildlife in North America...
  73. doi request reprint Isolate designation and characterization of Toxoplasma gondii isolates from pigs in the United States
    G V Velmurugan
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, Maryland 20705 2350, USA
    J Parasitol 95:95-9. 2009
    ..In conclusion, along with the predominance of clonal Type II and III strains, a few diverse, previously unrecognized T. gondii lineages were found circulating in domestic pigs used for human consumption...
  74. ncbi request reprint Canine neosporosis: clinical signs, diagnosis, treatment and isolation of Neospora caninum in mice and cell culture
    J P Dubey
    U S Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705 2350, USA
    Int J Parasitol 28:1293-304. 1998
    ..caninum. Furthermore, N. caninum (isolates NC-4, NC-5) was isolated in mice and in cell cultures inoculated with neural tissues of these two dogs. Serological diagnosis of neosporosis using a variety of tests is discussed...
  75. ncbi request reprint Characterization of the Oregon isolate of Neospora hughesi from a horse
    J P Dubey
    United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Maryland 20705 2350, USA
    J Parasitol 87:345-53. 2001
    ..caninum from dogs and cattle indicated that the Oregon isolate of N. hughesi is distinct from N. caninum isolates from cattle and dogs...
  76. ncbi request reprint Cryptosporidium, Giardia and Enterocytozoon bieneusi in cats from Bogota (Colombia) and genotyping of isolates
    Monica Santin
    Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Vet Parasitol 141:334-9. 2006
    ..bieneusi: genotype D-like (9%), K (4%), Peru 10 (2%), and Peru 5 (2%). This is the first report on the presence of zoonotic species/genotypes of Cryptosporidium and E. bieneusi in cats in Colombia...
  77. ncbi request reprint Isolation of Neospora caninum from naturally infected white-tailed deer (Odocoileus virginianus)
    M C B Vianna
    United States Department of Agriculture, Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, Beltsville Agricultural Research Center, Building 1001, Beltsville, MD 20705, USA
    Vet Parasitol 129:253-7. 2005
    ..This is the first record of in vitro isolation of N. caninum from white-tailed deer and lends credence to the white-tailed deer as an intermediate host for this parasite...
  78. ncbi request reprint Experimental infection of sheep with Neospora caninum oocysts
    Ryan O'Handley
    Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
    J Parasitol 88:1120-3. 2002
    ..Antibodies were detected by enzyme-linked immunosorbent assay, Neospora agglutination test, or immunoblotting to either native or recombinant N. caninum antigens in sheep inoculated with oocysts...
  79. doi request reprint Modest genetic differentiation among North American populations of Sarcocystis neurona may reflect expansion in its geographic range
    N Sundar
    United States Department of Agriculture, Agricultural Research Service, Animal Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
    Vet Parasitol 152:8-15. 2008
    ..Moreover, our study confirms that diverse intermediate hosts share a common infection source, the opossum (Didelphis virginiana)...
  80. ncbi request reprint Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig
    H R Gamble
    United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Building 1040, BARC East, Beltsville, MD 20705, USA
    Vet Parasitol 128:177-81. 2005
    ..0% specificity). Good correlation was seen between MAT and ELISA results. The results suggest that the ELISA may be a good tool for epidemiological studies of Toxoplasma infection on pig farms...
  81. ncbi request reprint Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in water buffaloes (Bubalus bubalis) from Egypt
    J P Dubey
    U S Department of Agriculture, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Laboratory, Beltsville, MD 20705 2350, USA
    Int J Parasitol 28:527-9. 1998
    ..gondii as antigen, indicating specificity in the detection of antibodies to N. caninum. This is the first report of N. caninum prevalence in water buffaloes, which are economically very important domestic animals in developing countries...
  82. ncbi request reprint A review of Neospora caninum and neosporosis
    J P Dubey
    Parasite Biology and Epidemiology Laboratory, US Department of Agriculture, BARC East, Beltsville, MD 20705 2350, USA
    Vet Parasitol 67:1-59. 1996
    ..It is also an important cause of neuromuscular paralysis in dogs. This paper reviews information on parasite structure, life cycle, biology, clinical signs, diagnosis, treatment and control...
  83. doi request reprint Seroepidemiology of Toxoplasma gondii infection in human adults from three rural communities in Durango State, Mexico
    C Alvarado-Esquivel
    Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, Durango, Dgo, Mexico
    J Parasitol 94:811-6. 2008
    ..22; 95% confidence interval [CI] = 1.11-16.05) and turkey meat (adjusted OR = 4.58; 95% CI = 1.14-18.44). This is the first epidemiological study of T. gondii prevalence in rural Mexico...
  84. doi request reprint Genotyping studies of Toxoplasma gondii isolates from Africa revealed that the archetypal clonal lineages predominate as in North America and Europe
    G V Velmurugan
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 155:314-8. 2008
    ..Taken together with recent studies of T. gondii isolates from Africa, it is clear that the three clonal lineages (Types I, II and III) predominate not only in North America and Europe, but also in Africa...
  85. ncbi request reprint Evaluation of vaccination with Neospora caninum protein for prevention of fetal loss associated with experimentally induced neosporosis in sheep
    Mark C Jenkins
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
    Am J Vet Res 65:1404-8. 2004
    ..To evaluate the immunologic response of a killed tachyzoite vaccine against Neospora caninum and its effectiveness in preventing fetal loss associated with experimentally induced neosporosis in sheep...
  86. ncbi request reprint Genotyping and detection of multiple infections of Toxoplasma gondii using Pyrosequencing
    C Sreekumar
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Int J Parasitol 35:991-9. 2005
    ..We conclude that Pyrosequencing offers a simple, rapid and efficient means for diagnosis and genotyping of T. gondii, as well as detection and quantification of multiple genotype infections of T. gondii...
  87. ncbi request reprint Effect of polyclonal antisera developed against dense granule-associated Neospora caninum proteins on cell invasion and development in vitro by N. caninum tachyzoites
    P C Augustine
    Parasite Biology and Epidemiology Laboratory, U S Department of Agriculture, Beltsville, Maryland 20705, USA
    Parasitology 119:441-5. 1999
    ..Addition of anti-DG sera at the time of inoculation of tachyzoites, or to the cell cultures after the parasites had entered cells, had little effect on either the percentage or rate of their subsequent development...
  88. ncbi request reprint Differential detection of Hammondia hammondi from Toxoplasma gondii using polymerase chain reaction
    C Sreekumar
    United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
    Parasitol Int 54:267-9. 2005
    ..hammondi from T. gondii even in samples containing both parasites, thus obviating the need for other labourious techniques like mouse bioassay and in vitro cultivation...
  89. ncbi request reprint Pathogenesis of bovine neosporosis
    J P Dubey
    Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, BARC East, Building 1001, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    J Comp Pathol 134:267-89. 2006
    ..Defining these immunological mechanisms will shed light on potential methods of control of bovine neosporosis and enrich our understanding of the continuity of mammalian and protozoal survival...
  90. ncbi request reprint Vaccination of ewes for prevention of vertical transmission of Neospora caninum
    R M O'Handley
    Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
    Am J Vet Res 64:449-52. 2003
    ..To evaluate the immunologic response of a killed tachyzoite vaccine against Neospora caninum and its effectiveness in preventing vertical transmission of N caninum in sheep...
  91. ncbi request reprint Specific detection of Neospora caninum oocysts in fecal samples from experimentally-infected dogs using the polymerase chain reaction
    D E Hill
    USDA, ARS, LPSI, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705, USA
    J Parasitol 87:395-8. 2001
    ..The assay should be useful in epidemiological surveys of both domestic and wild canine hosts and in investigations of oocyst biology in experimental infections...
  92. pmc Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissues
    L H Jauregui
    Immunology and Disease Resistance Laboratory, Epidemiology and Systematics Laboratory, ANRI, ARS, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA
    J Clin Microbiol 39:2065-71. 2001
    ..gondii in animal tissues...
  93. doi request reprint Seroepidemiology of Toxoplasma gondii infection in a Mennonite community in Durango State, Mexico
    C Alvarado-Esquivel
    Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Dgo, Mexico
    J Parasitol 96:941-5. 2010
    ..gondii infection in Mennonites and of an association of the consumption of pigeon meat with T. gondii infection. Results of this study should be useful in the design of optimal preventive measures against T. gondii infection...
  94. ncbi request reprint Immunization of mice with plasmid DNA coding for NcGRA7 or NcsHSP33 confers partial protection against vertical transmission of Neospora caninum
    Susan Liddell
    Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
    J Parasitol 89:496-500. 2003
    ..caninum positive, and none of the mice immunized with tachyzoite extract contained N. caninum DNA. Thus, immunization of mice with plasmid DNA expressing N. caninum antigens conferred partial protection against congenital neosporosis...
  95. ncbi request reprint A genetically diverse but distinct North American population of Sarcocystis neurona includes an overrepresented clone described by 12 microsatellite alleles
    Ingrid M Asmundsson
    Animal Parasitic Disease Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
    Infect Genet Evol 6:352-60. 2006
    ..Isolates associated with clinical neurological disease bear no obvious distinction as measured by these presumably neutral genetic markers...
  96. ncbi request reprint Genetic diversity among sea otter isolates of Toxoplasma gondii
    N Sundar
    United States Department of Agriculture, Agricultural Research Service, Animal Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
    Vet Parasitol 151:125-32. 2008
    ..The results from this study suggest that the sea otter isolates are genetically diverse...
  97. ncbi request reprint Toxoplasma gondii recombinant antigens H4 and H11: use in ELISAs for detection of toxoplasmosis in swine
    C D Andrews
    USDA, Food Safety and Inspection Service, Athens, GA 30605, USA
    Vet Parasitol 70:1-11. 1997
    ..gondii antigen extract. Although recombinant antigen ELISAs showed a sharp rise in response with some sera very early after infection, they were relatively non-reactive with late (chronic) infection sera...
  98. doi request reprint Toxoplasma gondii infection and schizophrenia: a case control study in a low Toxoplasma seroprevalence Mexican population
    Cosme Alvarado-Esquivel
    Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Dgo, Mexico
    Parasitol Int 60:151-5. 2011
    ..6) than in those with paranoid schizophrenia (F20.0) (P=0.02). This study provides elements to clarify the controversial information on the association of T. gondii infection and schizophrenia...
  99. ncbi request reprint Placentitis associated with leishmaniasis in a dog
    J P Dubey
    Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, USDA, Bldg 1001, Beltsville, MD 20705, USA
    J Am Vet Med Assoc 227:1266-9, 1250. 2005
    ..Protozoa were not found in the fetal tissues. An indirect fluorescent antibody test yielded a serum titer of 1:100, and a recombinant K39 immunoassay of serum yielded positive results for the K39 Leishmania antigen...
  100. pmc Localized multigene expression patterns support an evolving Th1/Th2-like paradigm in response to infections with Toxoplasma gondii and Ascaris suum
    Harry D Dawson
    Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Dept of Agriculture, Bldg 307C, Room 214, BARC East, Beltsville, MD 20705, USA
    Infect Immun 73:1116-28. 2005
    ....
  101. ncbi request reprint Disseminated toxoplasmosis in magpie geese (Anseranas semipalmata) with large numbers of tissue cysts in livers
    J P Dubey
    Livestock and Poultry Sciences Institute, Agricultural Research Service, Beltsville Agricultural Research Center, Maryland 20705-2350, USA
    J Parasitol 87:219-23. 2001
    ..Antibodies to T. gondii were found in 2 of 11 other geese from the zoo examined using the modified agglutination test. This is the first report of T. gondii infection in magpie geese (Anseranas semipalmata)...