Research Topics
| Jitender P DubeySummaryAffiliation: Agricultural Research Service Country: USA Publications
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Publications
Seroprevalence and isolation of Toxoplasma gondii from free-range chickens in Ghana, Indonesia, Italy, Poland, and VietnamJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 94:68-71. 2008..This is the first report of genetic characterization of T. gondii isolates from animals from these countries...
Ante-mortem diagnosis, diarrhea, oocyst shedding, treatment, isolation, and genetic typing of Toxoplasma gondii associated with clinical toxoplasmosis in a naturally infected catJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 99:158-60. 2013..4, which is grouped into the Type 12 lineage that is dominant in wildlife from North America. To our knowledge, this is the first T. gondii isolate characterized genetically from a sick cat in the USA...
High prevalence of Toxoplasma gondii antibodies in domestic pigs in Oaxaca State, MexicoC Alvarado-Esquivel
Faculty of Medicine and Nutrition, Juárez University of Durango State Avenida Universidad S N 34000 Durango, Mexico
J Parasitol 98:1248-50. 2012..Because there is no national system of determining the T. gondii infection status at the time of slaughter, precautions should be taken while handling pig carcasses, and all pork should be cooked thoroughly before human consumption...
Isolation of viable Toxoplasma gondii from tissues and feces of cats from Addis Ababa, EthiopiaJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 99:56-8. 2013..The high prevalence of T. gondii oocysts in feces of 8 (19.4%) of 36 cats is of high epidemiologic significance. This is the first report of isolation of viable T. gondii from any host in Ethiopia...
Sarcocystis neurona retinochoroiditis in a sea otter (Enhydra lutris kenyoni)J P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 183:156-9. 2011..Immunohistochemistry with S. neurona-specific polyclonal rabbit antibodies stained schizonts and merozoites. To our knowledge this is the first report of S. neurona-associated retinochoroiditis in any naturally infected animal...
Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiologyJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, BARC East, Beltsville, MD 20705 2350, USA
Parasitology 139:1375-424. 2012..The severity of clinical toxoplasmosis in Brazilian children may be associated with the genetic characteristics of T. gondii isolates prevailing in animals and humans in Brazil...
A review of toxoplasmosis in humans and animals in EthiopiaJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705, USA
Epidemiol Infect 140:1935-8. 2012..Serological surveys indicate up to 79% of goats and sheep have T. gondii antibodies. However, there is no information on losses due to toxoplasmosis in livestock or the presence of viable T. gondii in any host in Ethiopia...
Seroprevalence of Toxoplasma gondii Infection in Domestic Horses in Durango State, MexicoC Alvarado-Esquivel
Faculty of Medicine and Nutrition, Juárez University of Durango State, Avenida Universidad s n, 34000 Durango, Mexico E mail
J Parasitol 98:944-5. 2012..5%) than in pastured horses (3.1%). Results indicate that infected horses represent a potential source of T. gondii infection for humans in Mexico. This is the first report of T. gondii infection in horses in Mexico...
High prevalence and genotypes of Toxoplasma gondii isolated from organic pigs in northern USAJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 188:14-8. 2012..These results revealed very high prevalence of T. gondii in organic pigs for the first time in USA, indicating potentially increased health risk of consuming organic swine products...
Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out-bred, in-bred) on pathogenesis and mortalityJ P Dubey
United States Department of Agriculture, Animal Natural Resources Institute, Animal Parasitic Disease Laboratory, BARC East, Beltsville, MD 20705 2350, USA
Parasitology 139:1-13. 2012..gondii using the same preparations of oocysts which are stable for up to a year, and to have highly reproducible responses to the infection...
Whole genome sequencing of a natural recombinant Toxoplasma gondii strain reveals chromosome sorting and local allelic variantsIrene Lindström Bontell
Institute of Integrative and Comparative Biology, Clarendon Way, University of Leeds, Leeds LS2 9JT, UK
Genome Biol 10:R53. 2009..Little is known of the genotypes present in Africa despite the importance of AIDS-associated toxoplasmosis...
Mouse-virulent Toxoplasma gondii isolated from feral cats on Mona Island, Puerto RicoJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705, USA
J Parasitol 93:1365-9. 2007..The latter isolates of T. gondii were different biologically and phenotypically from the feline isolates from the rest of the Americas. One isolate (TgCatPr 12) was a mixed infection with 2 genotypes...
Toxoplasma gondii and Neospora caninum antibodies in dogs from Grenada, West IndiesJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
J Parasitol 94:750-1. 2008..Two of the 107 dogs had N. caninum antibodies (IFAT titers 1:100 and 1:400); these dogs had T. gondii titers of 1:1,600 and 1:50, respectively. Results indicate that these 2 structurally similar protozoa are antigenically different...
Seroprevalence of Neospora caninum and Toxoplasma gondii in black-tailed deer (Odocoileus hemionus columbianus) and mule deer (Odocoileus hemionus hemionus)J P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 156:310-3. 2008..T. gondii antibodies were found in 14 black-tailed deer but not in any of the mule deer. This is probably the first report of seroprevalence of N. caninum in these hosts...
Genetic diversity of Toxoplasma gondii isolates from chickens from BrazilJ P Dubey
United States Department of Agriculture, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
Vet Parasitol 157:299-305. 2008..Two isolates had mixed infections. Clonal Type II strain was absent. One strain was Type II at all loci, except BTUB. The results confirm high genetic diversity of T. gondii isolates from Brazil...
Isolation of Toxoplasma gondii from the keel-billed toucan (Ramphastos sulfuratus) from Costa RicaJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 95:467-8. 2009..The isolate designated TgRsCrl was found to be non-clonal with Type I, II, and III alleles at different loci. This is the first isolation of T. gondii from this host...
Isolation of Toxoplasma gondii from animals in Durango, MexicoJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705 2350, USA
J Parasitol 95:319-22. 2009..One genotype (the 4 chicken isolates) belongs to the clonal Type III lineage, three genotypes were reported in previous reports, and 1 genotype is unique...
Seroprevalence of Toxoplasma gondii and Bartonella spp. antibodies in cats from PennsylvaniaJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 95:578-80. 2009..gondii and Bartonella spp. seropositivity. Overall the seroprevalence of T. gondii antibodies in cats in this population is much lower compared with those reported in other localities in the United States and other countries...
The history of Toxoplasma gondii--the first 100 yearsJitender P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Eukaryot Microbiol 55:467-75. 2008..gondii oocysts washed from land. Hygiene remains the best preventive measure because currently there is no vaccine to prevent toxoplasmosis in humans...
Neospora caninum and Toxoplasma gondii antibodies in dogs from Durango City, MexicoJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
J Parasitol 93:1033-5. 2007..The N. caninum antibody titers of the 2 dogs were: 1:400 by IFAT and 1:200 by NAT in 1, and 1:25 by NAT and IFAT in the other. Results indicate that these 2 structurally similar protozoans are antigenically different...
Toxoplasmosis in captive dolphins (Tursiops truncatus) and walrus (Odobenus rosmarus)J P Dubey
Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705 2350, USA
J Parasitol 95:82-5. 2009..1 and the walrus showed archetypal alleles consistent with infection by a Type II strain. No unique polymorphisms were detected. This is apparently the first report of isolation of T. gondii from a marine mammal in Canada...
The evolution of the knowledge of cat and dog coccidiaJ P Dubey
Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705 2350, USA
Parasitology 136:1469-75. 2009..In the present paper I review salient features of the evolution of cat and dog coccidia...
Fatal intestinal coccidiosis in a three week old buffalo calf (Bubalus bubalus)J P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Beltsville, United States Department of Agriculture, Maryland, USA
J Parasitol 94:1289-94. 2008..No coccidian stages were seen in sections of colon. This is one of the first confirmed cases of clinical coccidiosis in water buffalo...
Toxoplasmosis in wallabies (Macropus rufogriseus and Macropus eugenii): blindness, treatment with atovaquone, and isolation of Toxoplasma gondiiJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705 2350, USA
J Parasitol 94:929-33. 2008..gondii from wallabies in the United States...
Isolation of Toxoplasma gondii from bottlenose dolphins (Tursiops truncatus)J P Dubey
Animal Parasitic Deseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705, USA
J Parasitol 94:821-3. 2008..Two of the 3 isolates have Type II alleles at all loci and belong to the clonal Type II lineage. One isolate has a unique genotype. This is the first report of isolation of viable T. gondii from T. truncatus...
Neosporosis in Beagle dogs: clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninumJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agricultural, Building 1001, Beltsville, MD 20705, USA
Vet Parasitol 149:158-66. 2007..caninum antibody titers were still persistent in these pups at 23 months of age. Results indicate that medication with Clindamycin can improve clinical condition but not eliminate N. caninum infection...
Characterization of Toxoplasma gondii from raccoons (Procyon lotor), coyotes (Canis latrans), and striped skunks (Mephitis mephitis) in Wisconsin identified several atypical genotypesJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705, USA
J Parasitol 93:1524-7. 2007..The results indicate the prevalence of T. gondii in wildlife mammals is high and that these animals may serve as an important reservoir for transmission of T. gondii...
Toxoplasma gondii infection in Blanford's fox (Vulpes cana)J P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 153:147-51. 2008..Protozoal tachyzoites reacted positively with T. gondii-specific polyclonal antibodies. Antibodies to T. gondii were detected in 10 of 12 V. cana assayed by the latex agglutination or the modified direct agglutination test...
Endemic toxoplasmosis in pigs on a farm in Maryland: isolation and genetic characterization of Toxoplasma gondiiJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 94:36-41. 2008..Both genotypes 1 and 2 are considered to belong to the clonal type II lineages. Genotype 3 and 4 are nonclonal isolates. Results document high prevalence of T. gondii in pigs on a farm in Maryland...
Isolation and genetic characterization of Toxoplasma gondii from raccoons (Procyon lotor), cats (Felis domesticus), striped skunk (Mephitis mephitis), black bear (Ursus americanus), and cougar (Puma concolor) from CanadaJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 94:42-5. 2008..These results are in contrast with the Type II genotype that is widespread in domestic animals and humans throughout the United States and Europe. This is the first genotyping of T. gondii isolates from this part of North America...
High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USAJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
Int J Parasitol 38:999-1006. 2008..gondii in lambs, which has important implications in public health. We believe this is the first in-depth genetic analysis of T. gondii isolates from sheep in the USA...
Toxoplasma gondii infection in humans and animals in the United StatesJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
Int J Parasitol 38:1257-78. 2008..Seroprevalence of T. gondii in humans and pigs is declining. Modes of transmission, epidemiology and environmental contamination with oocysts on land and sea are discussed...
A new species of Sarcocystis (Apicomplexa: Sarcocystidae) from the black bear (Ursus americanus)J P Dubey
Animal Parasitic Diseases Laboratory, USDA, Agricultural Research Service, Animal and Natural Resources Institute, BARC East, Building 1001, 10300 Baltimore Avenue, Beltsville, Maryland 20705 2350, USA
J Parasitol 94:496-9. 2008..0 microm long. The sarcocyst from the third bear was structurally different from S. ursusi; its sarcocyst wall was approximately 2 microm thick and had finger-like villi on the cyst wall giving the sarcocyst wall a striated appearance...
Transplacental toxoplasmosis in naturally-infected white-tailed deer: Isolation and genetic characterisation of Toxoplasma gondii from foetuses of different gestational agesJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
Int J Parasitol 38:1057-63. 2008..The most common genotype was Type II, commonly found in humans in North America and Europe, suggesting the possible link of transmission from game animals to humans...
Seroprevalence of Toxoplasma gondii and concurrent Bartonella spp., feline immunodeficiency virus, and feline leukemia virus infections in cats from Grenada, West IndiesJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, Maryland 20705 2350, USA
J Parasitol 95:1129-33. 2009..4% of 101 feral cats. Antibodies to FIV were found in 6 domestic and 22 feral cats. None of the 176 cats was positive for FeLv antigen. There was no correlation among T. gondii, Bartonella spp., and FIV seropositivity...
Toxoplasmosis in sheep--the last 20 yearsJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 163:1-14. 2009..In the present paper, information on the prevalence, transmission, and control of ovine toxoplasmosis in the last 20 years is reviewed...
Serological and parasitological prevalence of Toxoplasma gondii in wild birds from ColoradoJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 96:937-9. 2010..This is the first time T. gondii has been isolated from the barn owl, ferruginous hawk, rough-legged hawk, and Swainson's hawk...
Two new species of Sarcocystis (Apicomplexa: Sarcocystidae) infecting the wolverine (Gulo gulo) from Nunavut, CanadaJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 96:972-6. 2010..Sarcocystis kalvikus was found in 14 (34.1%), S. kitikmeotensis was found in 7 (17%), and both species were found in 12 (29.2%) of 41 wolverines...
Seroprevalence of Toxoplasma gondii infection in domestic pigs in Durango State, MexicoC Alvarado-Esquivel
Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Durango State, Mexico
J Parasitol 97:616-9. 2011..gondii infection of pigs in Mexico and the first report on pigs from Durango State, Mexico. Results indicate that infected pork is likely an important source of T. gondii infection for humans in Durango State...
Seroprevalence of Toxoplasma gondii and concurrent Bartonella spp., feline immunodeficiency virus, feline leukemia virus, and Dirofilaria immitis infections in Egyptian catsY M Al-Kappany
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350
J Parasitol 97:256-8. 2011..4%) cats, respectively. The results indicate a high prevalence of T. gondii, Bartonella spp., and FIV infections in cats from Cairo, Egypt. This is the first report of Bartonella spp., and D. immitis infection in cats in Egypt...
High prevalence and genotypes of Toxoplasma gondii isolated from goats, from a retail meat store, destined for human consumption in the USAJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Int J Parasitol 41:827-33. 2011..gondii in goats, which have important implications in public health. We believe this is the first genetic analysis of T. gondii isolates from goats in the USA...
Sporulation and survival of Toxoplasma gondii oocysts in different types of commercial cat litterJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 97:751-4. 2011..Oocysts sporulated within 2-3 days in all types of cat litters and occasionally remained viable for 14 days. Results indicate that cat litter should be changed daily to prevent sporulation and infectivity to people...
Gray wolf (Canis lupus) is a natural definitive host for Neospora caninumJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 181:382-7. 2011..Tissue stages in mice stained positively with N. caninum-specific polyclonal antibodies. Our observation suggests that wolves may be an important link in the sylvatic cycle of N. caninum...
Neosporosis in animals--the last five yearsJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
Vet Parasitol 180:90-108. 2011..This review is focused on current status of neosporosis in animals based on papers published in the last five years. Worldwide seroprevalences are tabulated. Strategies for control and prevention are discussed...
Genetic characterisation of Toxoplasma gondii in wildlife from North America revealed widespread and high prevalence of the fourth clonal typeJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Int J Parasitol 41:1139-47. 2011..No clonal Type I strain was identified among these wildlife isolates. These results suggest that T. gondii strains in wildlife from North America have limited diversity, with the occurrence of only a few major clonal types...
Isolation of viable Toxoplasma gondii from feral guinea fowl (Numida meleagris) and domestic rabbits (Oryctolagus cuniculus) from BrazilJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 97:842-5. 2011..This is the first genetic characterization of T. gondii isolates from the rabbits and guinea fowl in Brazil and the first host record for T. gondii in the guinea fowl...
Toxoplasmosis in Sand cats (Felis margarita) and other animals in the Breeding Centre for Endangered Arabian Wildlife in the United Arab Emirates and Al Wabra Wildlife Preservation, the State of QatarJ P Dubey
U S Department of Agriculture, Animal Natural Resources Institute, Animal Parasitic Disease Laboratory, Beltsville, MD 20705 2350, USA
Vet Parasitol 172:195-203. 2010..The genotype from the cat from AWWP (3657) is a genetic Type II strain with a Type I allele at locus Apico. This is the first report of genetic characterization of T. gondii isolates from Middle East...
Besnoitia neotomofelis n. sp. (Protozoa: Apicomplexa) from the southern plains woodrat ( Neotoma micropus)J P Dubey
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, BARC East, Beltsville, MD 20705 2350, USA
Parasitology 137:1731-47. 2010..Besnoitia neotomofelis is distinct from the 3 other species of Besnoitia, B. wallacei, B. darlingi and B. oryctofelisi that utilize cats as a definitive host...
Toxoplasmosis and other intestinal coccidial infections in cats and dogsJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Building 1001, Beltsville, MD 20705 2350, USA
Vet Clin North Am Small Anim Pract 39:1009-34, v. 2009..Much remains to be learned concerning the pathogenesis of clinical coccidiosis...
Morphologic and molecular characterization of the sarcocysts of Sarcocystis rileyi (Apicomplexa: Sarcocystidae) from the mallard duck ( Anas platyrhynchos )J P Dubey
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
J Parasitol 96:765-70. 2010....
A new atypical highly mouse virulent Toxoplasma gondii genotype isolated from a wild black bear in AlaskaJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, MD 20705 2350, USA
J Parasitol 96:713-6. 2010..DNA sequencing at intron loci EF1, HP2, and UPRT1 revealed that the TgBbUS1 is a divergent T. gondii strain. These results indicate that mouse-virulent atypical T. gondii genotypes are also circulating in wildlife in North America...
Isolation and characterization of viable Toxoplasma gondii isolates revealed possible high frequency of mixed infection in feral cats ( Felis domesticus) from St Kitts, West IndiesJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
Parasitology 136:589-94. 2009..Five of the 7 cats had infection with 2 genotypes, indicating high frequency of mixed infection in the cat population on the St Kitts island...
New Toxoplasma gondii genotypes isolated from free-range chickens from the Fernando de Noronha, Brazil: unexpected findingsJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, MD 20705 2350, USA
J Parasitol 96:709-12. 2010..Results in this study indicate that T. gondii on this island consists of unique genotypes as well as clonal genotypes that are dominant in Europe and North America...
Toxoplasmosis in pigs--the last 20 yearsJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 164:89-103. 2009..Recent studies on genetic typing of T. gondii strains prevalent in pigs are discussed with respect to epidemiology. Because wild pigs are hunted for food for human consumption prevalence in wild pigs is summarized...
Population biology of Toxoplasma gondii: what's out and where did they come fromJ P Dubey
Animal Parasitic Diseases Laboratory, ANRI, ARS, USDA, Beltsville, Maryland 20705 2350, USA
Mem Inst Oswaldo Cruz 104:190-5. 2009..No clonal type II lineage was found. Of the 253 isolates from animals from USA, 18 genotypes were identified, predominantly type II. These studies indicate a higher genetic diversity than previously recognized...
Toxoplasma gondii infections in chickens (Gallus domesticus): prevalence, clinical disease, diagnosis and public health significanceJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705 2350, USA
Zoonoses Public Health 57:60-73. 2010..A very high prevalence of the parasite was found in chickens raised in backyards (up to 100%) and free-range organic (30-50%) establishments...
History of the discovery of the life cycle of Toxoplasma gondiiJ P Dubey
United States Department of Agriculture, Animal and Natural Resources Institute, Beltsville, MD 20705 2350, USA
Int J Parasitol 39:877-82. 2009..The discovery of the environmentally resistant stage of the parasite, the oocyst, made it possible to explain its worldwide prevalence. In the present paper, events associated with the discovery of its life cycle are recalled...
Canine neosporosis: clinical signs, diagnosis, treatment and isolation of Neospora caninum in mice and cell cultureJ P Dubey
U S Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705 2350, USA
Int J Parasitol 28:1293-304. 1998..caninum. Furthermore, N. caninum (isolates NC-4, NC-5) was isolated in mice and in cell cultures inoculated with neural tissues of these two dogs. Serological diagnosis of neosporosis using a variety of tests is discussed...
Isolate designation and characterization of Toxoplasma gondii isolates from pigs in the United StatesG V Velmurugan
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, Maryland 20705 2350, USA
J Parasitol 95:95-9. 2009..In conclusion, along with the predominance of clonal Type II and III strains, a few diverse, previously unrecognized T. gondii lineages were found circulating in domestic pigs used for human consumption...
Characterization of the Oregon isolate of Neospora hughesi from a horseJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Maryland 20705 2350, USA
J Parasitol 87:345-53. 2001..caninum from dogs and cattle indicated that the Oregon isolate of N. hughesi is distinct from N. caninum isolates from cattle and dogs...
Isolation of Neospora caninum from naturally infected white-tailed deer (Odocoileus virginianus)M C B Vianna
United States Department of Agriculture, Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, Beltsville Agricultural Research Center, Building 1001, Beltsville, MD 20705, USA
Vet Parasitol 129:253-7. 2005..This is the first record of in vitro isolation of N. caninum from white-tailed deer and lends credence to the white-tailed deer as an intermediate host for this parasite...
Cryptosporidium, Giardia and Enterocytozoon bieneusi in cats from Bogota (Colombia) and genotyping of isolatesMonica Santin
Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Building 173, BARC East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
Vet Parasitol 141:334-9. 2006..bieneusi: genotype D-like (9%), K (4%), Peru 10 (2%), and Peru 5 (2%). This is the first report on the presence of zoonotic species/genotypes of Cryptosporidium and E. bieneusi in cats in Colombia...
Modest genetic differentiation among North American populations of Sarcocystis neurona may reflect expansion in its geographic rangeN Sundar
United States Department of Agriculture, Agricultural Research Service, Animal Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705 2350, USA
Vet Parasitol 152:8-15. 2008..Moreover, our study confirms that diverse intermediate hosts share a common infection source, the opossum (Didelphis virginiana)...
Experimental infection of sheep with Neospora caninum oocystsSusan Liddell
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
J Parasitol 88:1120-3. 2002..Antibodies were detected by enzyme-linked immunosorbent assay, Neospora agglutination test, or immunoblotting to either native or recombinant N. caninum antigens in sheep inoculated with oocysts...
Genotyping studies of Toxoplasma gondii isolates from Africa revealed that the archetypal clonal lineages predominate as in North America and EuropeG V Velmurugan
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 155:314-8. 2008..Taken together with recent studies of T. gondii isolates from Africa, it is clear that the three clonal lineages (Types I, II and III) predominate not only in North America and Europe, but also in Africa...
Seroepidemiology of Toxoplasma gondii infection in human adults from three rural communities in Durango State, MexicoC Alvarado-Esquivel
Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, Durango, Dgo, Mexico
J Parasitol 94:811-6. 2008..22; 95% confidence interval [CI] = 1.11-16.05) and turkey meat (adjusted OR = 4.58; 95% CI = 1.14-18.44). This is the first epidemiological study of T. gondii prevalence in rural Mexico...
A review of Neospora caninum and neosporosisJ P Dubey
Parasite Biology and Epidemiology Laboratory, US Department of Agriculture, BARC East, Beltsville, MD 20705 2350, USA
Vet Parasitol 67:1-59. 1996..It is also an important cause of neuromuscular paralysis in dogs. This paper reviews information on parasite structure, life cycle, biology, clinical signs, diagnosis, treatment and control...
Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pigH R Gamble
United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Building 1040, BARC East, Beltsville, MD 20705, USA
Vet Parasitol 128:177-81. 2005..0% specificity). Good correlation was seen between MAT and ELISA results. The results suggest that the ELISA may be a good tool for epidemiological studies of Toxoplasma infection on pig farms...
Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in water buffaloes (Bubalus bubalis) from EgyptJ P Dubey
U S Department of Agriculture, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Laboratory, Beltsville, MD 20705 2350, USA
Int J Parasitol 28:527-9. 1998..gondii as antigen, indicating specificity in the detection of antibodies to N. caninum. This is the first report of N. caninum prevalence in water buffaloes, which are economically very important domestic animals in developing countries...
Genotyping and detection of multiple infections of Toxoplasma gondii using PyrosequencingC Sreekumar
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705-2350, USA
Int J Parasitol 35:991-9. 2005..We conclude that Pyrosequencing offers a simple, rapid and efficient means for diagnosis and genotyping of T. gondii, as well as detection and quantification of multiple genotype infections of T. gondii...
Differential detection of Hammondia hammondi from Toxoplasma gondii using polymerase chain reactionC Sreekumar
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA
Parasitol Int 54:267-9. 2005..hammondi from T. gondii even in samples containing both parasites, thus obviating the need for other labourious techniques like mouse bioassay and in vitro cultivation...
Evaluation of vaccination with Neospora caninum protein for prevention of fetal loss associated with experimentally induced neosporosis in sheepMark C Jenkins
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
Am J Vet Res 65:1404-8. 2004..CONCLUSIONS AND CLINICAL RELEVANCE: The N caninum tachyzoite vaccine used in this study appeared to provide protection against fetal loss associated with experimentally induced neosporosis in a high proportion of pregnant ewes...
Vaccination of ewes for prevention of vertical transmission of Neospora caninumS A Morgan
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
Am J Vet Res 64:449-52. 2003..CONCLUSIONS AND CLINICAL RELEVANCE: The killed tachyzoite vaccine against N caninum stimulated a humoral immune response in sheep and provided partial protection against vertical transmission...
Effect of polyclonal antisera developed against dense granule-associated Neospora caninum proteins on cell invasion and development in vitro by N. caninum tachyzoitesP C Augustine
Parasite Biology and Epidemiology Laboratory, U S Department of Agriculture, Beltsville, Maryland 20705, USA
Parasitology 119:441-5. 1999..Addition of anti-DG sera at the time of inoculation of tachyzoites, or to the cell cultures after the parasites had entered cells, had little effect on either the percentage or rate of their subsequent development...
Pathogenesis of bovine neosporosisJ P Dubey
Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, BARC-East, Building 1001, 10300 Baltimore Avenue, Beltsville, MD 20705-2350, USA
J Comp Pathol 134:267-89. 2006..Defining these immunological mechanisms will shed light on potential methods of control of bovine neosporosis and enrich our understanding of the continuity of mammalian and protozoal survival...
A genetically diverse but distinct North American population of Sarcocystis neurona includes an overrepresented clone described by 12 microsatellite allelesIngrid M Asmundsson
Animal Parasitic Disease Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
Infect Genet Evol 6:352-60. 2006..Isolates associated with clinical neurological disease bear no obvious distinction as measured by these presumably neutral genetic markers...
Genetic diversity among sea otter isolates of Toxoplasma gondiiN Sundar
United States Department of Agriculture, Agricultural Research Service, Animal Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, MD 20705 2350, USA
Vet Parasitol 151:125-32. 2008..The results from this study suggest that the sea otter isolates are genetically diverse...
Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissuesL H Jauregui
Immunology and Disease Resistance Laboratory, Epidemiology and Systematics Laboratory, ANRI, ARS, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA
J Clin Microbiol 39:2065-71. 2001..gondii in animal tissues...
Immunization of mice with plasmid DNA coding for NcGRA7 or NcsHSP33 confers partial protection against vertical transmission of Neospora caninumSusan Liddell
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA
J Parasitol 89:496-500. 2003..caninum positive, and none of the mice immunized with tachyzoite extract contained N. caninum DNA. Thus, immunization of mice with plasmid DNA expressing N. caninum antigens conferred partial protection against congenital neosporosis...
Specific detection of Neospora caninum oocysts in fecal samples from experimentally-infected dogs using the polymerase chain reactionD E Hill
USDA, ARS, LPSI, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705, USA
J Parasitol 87:395-8. 2001..The assay should be useful in epidemiological surveys of both domestic and wild canine hosts and in investigations of oocyst biology in experimental infections...
Seroepidemiology of Toxoplasma gondii infection in a Mennonite community in Durango State, MexicoC Alvarado-Esquivel
Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Dgo, Mexico
J Parasitol 96:941-5. 2010..gondii infection in Mennonites and of an association of the consumption of pigeon meat with T. gondii infection. Results of this study should be useful in the design of optimal preventive measures against T. gondii infection...
Toxoplasma gondii recombinant antigens H4 and H11: use in ELISAs for detection of toxoplasmosis in swineC D Andrews
USDA, Food Safety and Inspection Service, Athens, GA 30605, USA
Vet Parasitol 70:1-11. 1997..gondii antigen extract. Although recombinant antigen ELISAs showed a sharp rise in response with some sera very early after infection, they were relatively non-reactive with late (chronic) infection sera...
Toxoplasma gondii infection and schizophrenia: a case control study in a low Toxoplasma seroprevalence Mexican populationCosme Alvarado-Esquivel
Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Dgo, Mexico
Parasitol Int 60:151-5. 2011..6) than in those with paranoid schizophrenia (F20.0) (P=0.02). This study provides elements to clarify the controversial information on the association of T. gondii infection and schizophrenia...
Seroepidemiology of Toxoplasma gondii infection in pregnant women in rural Durango, MexicoC Alvarado-Esquivel
Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Dgo, Mexico
J Parasitol 95:271-4. 2009..6%). Multivariate analysis showed that T. gondii infection was associated with soil floors at home (adjusted OR = 2.89; 95% CI: 1.12-7.49). This is the first epidemiological study of T. gondii infection in pregnant women in rural Mexico...
Localized multigene expression patterns support an evolving Th1/Th2-like paradigm in response to infections with Toxoplasma gondii and Ascaris suumHarry D Dawson
Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Dept. of Agriculture, Bldg. 307C, Room 214, BARC-East, Beltsville, MD 20705, USA
Infect Immun 73:1116-28. 2005....
Effect of gamma irradiation on unsporulated and sporulated Toxoplasma gondii oocystsJ P Dubey
U S Department of Agriculture, Beltsville Agriculture Research Center, MD 20705 2350, USA
Int J Parasitol 28:369-75. 1998..Raspberries inoculated with sporulated T. gondii oocysts were rendered innocuous after irradiation at 0.4 kGy. Results indicate that irradiation at 0.5 kGy is effective in "killing" coccidian oocysts on fruits and vegetables...
Validation of the specificity of the modified agglutination test for toxoplasmosis in pigsJ P Dubey
Parasite Biology and Epidemiology Laboratory, US Department of Agriculture, Beltsville, MD 20705 2350, USA
Vet Parasitol 71:307-10. 1997..Antibodies were also not found in 1:25 dilution of sera from 200 naturally aborted fetal pigs. Thus, evidence for cross reactivity to T. gondii with unrelated or related organisms using the MAT was not found...
Placentitis associated with leishmaniasis in a dogJ P Dubey
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, USDA, Bldg 1001, Beltsville, MD 20705, USA
J Am Vet Med Assoc 227:1266-9, 1250. 2005..Protozoa were not found in the fetal tissues. An indirect fluorescent antibody test yielded a serum titer of 1:100, and a recombinant K39 immunoassay of serum yielded positive results for the K39 Leishmania antigen...
Disseminated toxoplasmosis in magpie geese (Anseranas semipalmata) with large numbers of tissue cysts in liversJ P Dubey
Livestock and Poultry Sciences Institute, Agricultural Research Service, Beltsville Agricultural Research Center, Maryland 20705-2350, USA
J Parasitol 87:219-23. 2001..Antibodies to T. gondii were found in 2 of 11 other geese from the zoo examined using the modified agglutination test. This is the first report of T. gondii infection in magpie geese (Anseranas semipalmata)...
Re-examination of resistance of Toxoplasma gondii tachyzoites and bradyzoites to pepsin and trypsin digestionJ P Dubey
United States Department of Agriculture, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705 2350, USA
Parasitology 116:43-50. 1998..5% trypsin solution was better than in acid pepsin solution. The resistance to digestion in acid pepsin solution is not a reliable method to distinguish tachyzoites from bradyzoites...
Acute Sarcocystis falcatula-like infection in a carmine bee-eater (Merops nubicus) and immunohistochemical cross reactivity between Sarcocystis falcatula and Sarcocystis neuronaJ P Dubey
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705-2350, USA
J Parasitol 87:824-32. 2001..falcatula and Sarcocystis neurona. Results indicated that sarcocysts in the bee-eater were morphologically different from the reported structure for sarcocysts of other S. falcatula infections...
Cryptosporidium canis n. sp. from domestic dogsR Fayer
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville, Maryland 20705-2350, USA
J Parasitol 87:1415-22. 2001..The differences in genetics and host specificity clearly differentiate C. canis as a new species...
Zoonotic protozoa: from land to seaRonald Fayer
Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
Trends Parasitol 20:531-6. 2004..This review addresses the extent of contamination and the animals affected by three genera of important zoonotic protozoa: Giardia, Cryptosporidium and Toxoplasma...
High prevalence of toxoplasmosis in cats from Egypt: isolation of viable Toxoplasma gondii, tissue distribution, and isolate designationY M Al-Kappany
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 96:1115-8. 2010..The high prevalence of T. gondii in feral cats in Egypt reported here indicates a high environmental contamination with oocysts...
Genetic diversity of Toxoplasma gondii isolates in Egyptian feral cats reveals new genotypesY M Al-Kappany
U S Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705 2350, USA
J Parasitol 96:1112-4. 2010..Four isolates had mixed infections. These results revealed a strong clonal population structure with the dominance of clonal Type II and III lineages of T. gondii in feral cats from Egypt...
Seroepidemiology of Toxoplasma gondii infection in general population in a northern Mexican cityC Alvarado-Esquivel
Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Durango, Mexico
J Parasitol 97:40-3. 2011..gondii infection. This study provides a basis for the design of successful preventive measures against T. gondii infection...
Prevalence of Toxoplasma gondii infection in wild birds in Durango, MexicoC Alvarado-Esquivel
Faculty of Medicine, Juárez University of Durango State, Avenida Universidad S N esquina Fanny Anitua, 34000 Durango, Dgo, Mexico
J Parasitol 97:809-12. 2011..This is the first report of T. gondii infection in great-tailed grackles, the Mexican duck, and curved-billed thrashers and the first survey of wild birds in Mexico...
Clinical muscular sarcocystosis in a dogJ Chapman
Department of Veterinary Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA
J Parasitol 91:187-90. 2005..The sarcocyst wall was approximately 1 microm thick and contained short, stubby, villar protrusions that lacked microtubules. This is the first report on clinical muscular sarcocystosis in a dog...
Seroprevalence of Toxoplasma gondii antibodies in the rodent capybara (Hidrochoeris hidrochoeris) from BrazilW A Cañón-Franco
Departamento de Medicina Veterinaria Preventiva e Saude Animal, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo, Avenida Prof Orlando Marques de Paiva, 87, Cidade Universitaria, Sao Paulo, São Paulo CEP 05508 000, Brazil
J Parasitol 89:850. 2003..Using IFAT, antibodies (>1:16) were found in 104 (69.8%) and with the MAT, antibodies (>1:25) were found in 63 (42.3%) capybaras. This is the first report of prevalence of T. gondii antibodies in this host...
Prevalence of Toxoplasma gondii in chickens from an area in southern Brazil highly endemic to humansDaniele Seipel da Silva
, , Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes 28013-600, Brazil
J Parasitol 89:394-6. 2003..Thus, the high recovery rate of T. gondii observed in mice is indicative of high levels of environmental contamination of free-range chickens by T. gondii oocysts in this area that is endemic to humans...
Transmission dynamics of Toxoplasma gondii on a pig farmTovi Lehmann
Division of Parasitic Diseases MS F22, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30041, USA
Infect Genet Evol 3:135-41. 2003....
