S E Dowd

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. pmc Characterization of a newly developed chicken 44K Agilent microarray
    Xianyao Li
    Texas Agricultural Experiment Station, Texas A and M University, College Station, TX, USA
    BMC Genomics 9:60. 2008
  2. pmc The rehydration transcriptome of the desiccation-tolerant bryophyte Tortula ruralis: transcript classification and analysis
    Melvin J Oliver
    Cropping Systems Research Laboratory, USDA ARS, 3810 4th St, Lubbock, TX, USA
    BMC Genomics 5:89. 2004
  3. doi request reprint Evaluation of gene expression in a single antibiotic exposure-derived isolate of Salmonella enterica typhimurium 14028 possessing resistance to multiple antibiotics
    Scot E Dowd
    Livestock Issues Research Unit, U S Department of Agriculture, Agricultural Research Service, Lubbock, Texas 79403, USA
    Foodborne Pathog Dis 5:205-21. 2008
  4. ncbi request reprint Escherichia coli O157:H7 gene expression in the presence of catecholamine norepinephrine
    Scot E Dowd
    United States Department of Agriculture, Agriculture Research Service, Livestock Issues Research Unit, Lubbock, TX 79403, USA
    FEMS Microbiol Lett 273:214-23. 2007
  5. ncbi request reprint Handling may cause increased shedding of Escherichia coli and total coliforms in pigs
    Scot E Dowd
    Livestock Issues Research Unit, USDA Agricultural Research Service, Lubbock, TX 79403, USA
    Foodborne Pathog Dis 4:99-102. 2007
  6. pmc Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing
    Scot E Dowd
    United States Department of Agriculture ARS Livestock Issues Research Unit, Lubbock, TX, USA
    BMC Microbiol 8:43. 2008
  7. ncbi request reprint Comparison of Shiga-like toxin II expression between two genetically diverse lineages of Escherichia coli O157:H7
    Scot E Dowd
    U S Department of Agriculture, Agricultural Research Service Livestock Issues Research Unit, 1604 West FM 1294, Lubbock, Texas 79403, USA
    J Food Prot 71:1673-8. 2008
  8. pmc Microarray based comparison of two Escherichia coli O157:H7 lineages
    Scot E Dowd
    Livestock Issues Research Unit, USDA ARS, Lubbock, TX, USA
    BMC Microbiol 6:30. 2006
  9. pmc Windows .NET Network Distributed Basic Local Alignment Search Toolkit (W.ND-BLAST)
    Scot E Dowd
    Livestock Issues Research Unit, Agriculture Research Service, USDA, Lubbock, TX, USA
    BMC Bioinformatics 6:93. 2005
  10. ncbi request reprint Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model
    S E Dowd
    US Department of Agriculture ARS Livestock Issues Research Unit, Lubbock, Texas, USA
    J Wound Care 18:508, 510-12. 2009

Detail Information

Publications49

  1. pmc Characterization of a newly developed chicken 44K Agilent microarray
    Xianyao Li
    Texas Agricultural Experiment Station, Texas A and M University, College Station, TX, USA
    BMC Genomics 9:60. 2008
    ..This provides a flexible and powerful platform with high sensitivity and specificity for gene expression studies...
  2. pmc The rehydration transcriptome of the desiccation-tolerant bryophyte Tortula ruralis: transcript classification and analysis
    Melvin J Oliver
    Cropping Systems Research Laboratory, USDA ARS, 3810 4th St, Lubbock, TX, USA
    BMC Genomics 5:89. 2004
    ....
  3. doi request reprint Evaluation of gene expression in a single antibiotic exposure-derived isolate of Salmonella enterica typhimurium 14028 possessing resistance to multiple antibiotics
    Scot E Dowd
    Livestock Issues Research Unit, U S Department of Agriculture, Agricultural Research Service, Lubbock, Texas 79403, USA
    Foodborne Pathog Dis 5:205-21. 2008
    ..Future work in sequencing these genomes and evaluating pathogenicity are suggested...
  4. ncbi request reprint Escherichia coli O157:H7 gene expression in the presence of catecholamine norepinephrine
    Scot E Dowd
    United States Department of Agriculture, Agriculture Research Service, Livestock Issues Research Unit, Lubbock, TX 79403, USA
    FEMS Microbiol Lett 273:214-23. 2007
    ..coli O157:H7. The results corroborate many of the previous findings detailed in the literature and provide new observations that could expand the scope of microbial endocrinology...
  5. ncbi request reprint Handling may cause increased shedding of Escherichia coli and total coliforms in pigs
    Scot E Dowd
    Livestock Issues Research Unit, USDA Agricultural Research Service, Lubbock, TX 79403, USA
    Foodborne Pathog Dis 4:99-102. 2007
    ..coli and total coliforms, possibly due to stress, and these populations of bacteria may have potential utility as inexpensive, noninvasive indicators of handling-related stress in pigs...
  6. pmc Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing
    Scot E Dowd
    United States Department of Agriculture ARS Livestock Issues Research Unit, Lubbock, TX, USA
    BMC Microbiol 8:43. 2008
    ....
  7. ncbi request reprint Comparison of Shiga-like toxin II expression between two genetically diverse lineages of Escherichia coli O157:H7
    Scot E Dowd
    U S Department of Agriculture, Agricultural Research Service Livestock Issues Research Unit, 1604 West FM 1294, Lubbock, Texas 79403, USA
    J Food Prot 71:1673-8. 2008
    ..coli O157 populations to differentiate these lineages. This study provides useful information on the ecology of E. coli O157, with broad implications within the clinical, scientific, and livestock industries...
  8. pmc Microarray based comparison of two Escherichia coli O157:H7 lineages
    Scot E Dowd
    Livestock Issues Research Unit, USDA ARS, Lubbock, TX, USA
    BMC Microbiol 6:30. 2006
    ..To determine if there are virulence related genes differentially expressed between the two lineages we chose to utilize microarray technology to perform an initial screening...
  9. pmc Windows .NET Network Distributed Basic Local Alignment Search Toolkit (W.ND-BLAST)
    Scot E Dowd
    Livestock Issues Research Unit, Agriculture Research Service, USDA, Lubbock, TX, USA
    BMC Bioinformatics 6:93. 2005
    ..Our goal was to develop an easy to use, fault tolerant, high-throughput BLAST solution that incorporates a comprehensive BLAST result viewer with curation and annotation functionality...
  10. ncbi request reprint Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model
    S E Dowd
    US Department of Agriculture ARS Livestock Issues Research Unit, Lubbock, Texas, USA
    J Wound Care 18:508, 510-12. 2009
    ..To evaluate the efficacy of several biofilm effectors in inhibiting biofilm formation in an in vitro multi-species chronic wound biofilm model...
  11. ncbi request reprint Survey of fungi and yeast in polymicrobial infections in chronic wounds
    S E Dowd
    Research and Testing Laboratory, Lubbock, TX, USA
    J Wound Care 20:40-7. 2011
    ..To assess the incidence, abundance and species diversity of fungi in chronic wounds, as well as to describe the associations of major fungi populations...
  12. ncbi request reprint Biofilm maturity studies indicate sharp debridement opens a time- dependent therapeutic window
    R D Wolcott
    Southwest Regional Wound Care Center, Lubbock, TX, USA
    J Wound Care 19:320-8. 2010
    ..To investigate the hypothesis that newly formed wound biofilms (or bioburdens) are more susceptible to antimicrobial treatment...
  13. ncbi request reprint Propagation of anaerobic bacteria within an aerobic multi-species chronic wound biofilm model
    Y Sun
    Research and Testing Laboratory, Lubbock, TX, USA
    J Wound Care 18:426-31. 2009
    ..In order to better screen antimicrobial and antibiofilm therapeutics, we evaluated the ability of key anaerobes to incorporate and propagate within our aerobic chronic wound biofilm...
  14. doi request reprint Microarray analysis and draft genomes of two Escherichia coli O157:H7 lineage II cattle isolates FRIK966 and FRIK2000 investigating lack of Shiga toxin expression
    Scot E Dowd
    Research and Testing Laboratory, Lubbock, Texas 79407, USA
    Foodborne Pathog Dis 7:763-73. 2010
    ..The microarray and genome data have been made available to the scientific community to allow continuing analysis of these cattle-isolated lineage II genomes and their gene expression...
  15. ncbi request reprint A rapid molecular method for characterising bacterial bioburden in chronic wounds
    R D Wolcott
    Southwest Regional Wound Care Center, Lubbock, TX, USA
    J Wound Care 17:513-6. 2008
    ..This case study describes how, unlike traditional culture reports, a modification of this technique rapidly determined that Pseudomonas spp. was present in a chronic wound...
  16. ncbi request reprint Bacterial diversity in surgical site infections: not just aerobic cocci any more
    R D Wolcott
    Southwest Regional Wound Care Centre, Lubbock, Texas, USA
    J Wound Care 18:317-23. 2009
    ..To evaluate the microbial diversity in chronic surgical site infections (SSIs)...
  17. ncbi request reprint Molecular diagnostics and personalised medicine in wound care: assessment of outcomes
    S E Dowd
    Research and Testing Laboratory and Pathogenius Diagnostics, Lubbock, TX, USA
    J Wound Care 20:232, 234-9. 2011
    ....
  18. doi request reprint Evaluation of bacterial diversity in the rumen and feces of cattle fed different levels of dried distillers grains plus solubles using bacterial tag-encoded FLX amplicon pyrosequencing
    T R Callaway
    Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, USDA, College Station, TX 77845, USA
    J Anim Sci 88:3977-83. 2010
    ..Using bTEFAP, the normal microbiota of cattle were examined using modern molecular methods to understand how diets affect gastrointestinal ecology and the gastrointestinal contribution of the microbiome to animal health and production...
  19. doi request reprint Development of colonic microflora as assessed by pyrosequencing in dairy calves fed waste milk
    T S Edrington
    Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, USDA ARS, College Station, TX 77845, USA
    J Dairy Sci 95:4519-25. 2012
    ..6 and 1.3%). The consistent detection of Salmonella in the younger animals fed nonpasteurized milk and its absence in all other groups is an important finding related to this feeding practice...
  20. ncbi request reprint Identification of yeast in chronic wounds using new pathogen-detection technologies
    J L Leake
    Texas Tech University Howard Hughes Medical Institute Program, Lubbock, Texas, USA
    J Wound Care 18:103-4, 106, 108. 2009
    ..To evaluate the ability of two new diagnostic methods to detect and accurately identify yeast associated with chronic wound infections...
  21. ncbi request reprint Healing and healing rates of chronic wounds in the age of molecular pathogen diagnostics
    R D Wolcott
    Southwest Regional Wound Care Center, Lubbock, Texas, USA
    J Wound Care 19:272-8, 280-1. 2010
    ..To compare healing outcomes at a wound healing centre both before and after the introduction of molecular pathogen diagnostics...
  22. ncbi request reprint Chronic wounds and the medical biofilm paradigm
    R D Wolcott
    Southwest Regional Wound Care Center, Lubbock, Texas, USA
    J Wound Care 19:45-6, 48-50, 52-3. 2010
    ..There is a growing recognition that biofilms are the principal cause of wound chronicity. The development of treatments for wound biofilms raises the prospect that chronic wounds can be treated, potentially saving many patients' lives...
  23. pmc Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)
    Scot E Dowd
    USDA ARS Livestock Issues Research Unit, Lubbock, TX 79403, USA
    BMC Microbiol 8:125. 2008
    ..We have evaluated the microbiome from the feces of 20 commercial, lactating dairy cows...
  24. ncbi request reprint Regular debridement is the main tool for maintaining a healthy wound bed in most chronic wounds
    R D Wolcott
    Southwest Regional Wound Care Center, Lubbock, Texas, USA
    J Wound Care 18:54-6. 2009
    ..Sharp debridement is the most clinically and cost-effective way of physically removing and suppressing a biofilm. Continued debridement, as part of a multifaceted treatment strategy, will keep the biofilm in a weakened state...
  25. pmc Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP)
    Scot E Dowd
    Research and Testing Laboratory, Lubbock, Texas, USA
    PLoS ONE 3:e3326. 2008
    ..Diabetic foot ulcers are the most common, disabling, and costly complications of diabetes. Here we seek to derive a better understanding of the polymicrobial nature of chronic diabetic extremity ulcer infections...
  26. pmc The effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16S rRNA gene sequencing
    Jan S Suchodolski
    Gastrointestinal Laboratory, Texas A and M University, College Station, TX, USA
    BMC Microbiol 9:210. 2009
    ..Jejunal brush samples were collected through the fistula on days 0, 14, and 28 (14 days after withdrawal of tylosin). Bacterial diversity was characterized using massive parallel 16S rRNA gene pyrosequencing...
  27. ncbi request reprint EST-based gene indices for the cattle fever tick, the horn fly, and the screwworm fly
    F D Guerrero
    USDA ARS Knipling Bushland US Livestock Insects Research Lab, Kerrville, Texas 78028, USA
    Dev Biol (Basel) 132:115-20. 2008
    ..Several putative tick G protein-coupled receptors (GPCRs) were identified that mightserve as starting points to develop novel control technologies...
  28. ncbi request reprint Expressed cDNAS from embryonic and larval stages of the horn fly (Diptera: Muscidae)
    F D Guerrero
    USDA ARS, Knipling Bushland U S Livestock Insects Research Laboratory 2700 Fredericksburg Rd, Kerrville, TX 78028, USA
    J Med Entomol 45:686-92. 2008
    ..This would include studies designed to investigate metabolic-based insecticide resistance, identify novel antigens for vaccine-based control approaches, and discover new proteins to serve as targets for new pesticide development...
  29. doi request reprint Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination
    Pia Untalan Olafson
    USDA ARS, Knipling Bushland U S Livestock Insects Research Laboratory, Kerrville, Texas 78028, USA
    Arch Insect Biochem Physiol 74:179-204. 2010
    ..The current database will be a valuable tool for target identification and for comparative studies with other Diptera...
  30. pmc Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and titanium amplicon pyrosequencing and metagenomic approaches
    Randall D Wolcott
    Southwest Regional Wound Care Clinic, Lubbock, TX 79410, USA
    BMC Microbiol 9:226. 2009
    ..There are known underlying pathologies which contribute to the chronic nature of VLU including biofilm phenotype infections...
  31. pmc Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing
    Renato Andreotti
    USDA ARS Knipling Bushland U S Livestock Insects Research Laboratory, 2700 Fredericksburg Rd, Kerrville, TX 78028, USA
    BMC Microbiol 11:6. 2011
    ..Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas...
  32. doi request reprint Choline and osmotic-stress tolerance induced in Arabidopsis by the soil microbe Bacillus subtilis (GB03)
    Huiming Zhang
    Department of Chemistry, Texas Tech University, Lubbock, TX, USA
    Mol Plant Microbe Interact 23:1097-104. 2010
    ..These results provide a biological strategy to enhance Cho biosynthesis in plants and, in turn, increase plant tolerance to osmotic stress by elevating osmoprotectant accumulation...
  33. doi request reprint Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) for microbiome studies: bacterial diversity in the ileum of newly weaned Salmonella-infected pigs
    Scot E Dowd
    USDA ARS Livestock Issues Research Unit, Lubbock, Texas 79403, USA
    Foodborne Pathog Dis 5:459-72. 2008
    ..Using bTEFAP, we can expect to gain a better understanding of how the microbiome of an animal contributes to its health and well-being...
  34. doi request reprint Iron regulated genes of Salmonella enterica serovar Typhimurium in response to norepinephrine and the requirement of fepDGC for norepinephrine-enhanced growth
    Bradley L Bearson
    Swine Odor and Manure Management Research Unit, USDA, ARS, National Soil Tilth Laboratory, Ames, IA 50011, USA
    Microbes Infect 10:807-16. 2008
    ..Contrary to previous reports using small animal models, the fepA iroN cirA mutant of Salmonella Typhimurium colonized the swine gastrointestinal tract, as did the fepC mutant...
  35. ncbi request reprint Positive adaptive state: microarray evaluation of gene expression in Salmonella enterica Typhimurium exposed to nalidixic acid
    Scot E Dowd
    United States Department of Agriculture, Agricultural Research Service, Livestock Issues Research Unit, Lubbock, Texas 79403, USA
    Foodborne Pathog Dis 4:187-200. 2007
    ..Evaluation of gene expression profile changes that occur during exposure to antibiotics will continue to improve our understanding of the development of antibiotic resistance...
  36. ncbi request reprint Planktonic and biofilm community characterization and Salmonella resistance of 14-day-old chicken cecal Microflora-derived continuous-flow cultures
    Tawni L Crippen
    Southern Plains Agricultural Research Center, Agricultural Research Service, U S Department of Agriculture, College Station, Texas 77845, USA
    J Food Prot 71:1981-7. 2008
    ..These results describe bacterial mixtures containing less diversity than in previously described avian protective cultures...
  37. doi request reprint A combined subtractive suppression hybridization and expression profiling strategy to identify novel desiccation response transcripts from Tortula ruralis gametophytes
    Melvin J Oliver
    USDA ARS MWA, Plant Genetics Research Unit, University of Missouri, Columbia, MO 65211, USA
    Physiol Plant 136:437-60. 2009
    ..These are novel findings and have relevance to our understanding of the evolution of desiccation tolerance in the land plants...
  38. ncbi request reprint Microarray analysis of female- and larval-specific gene expression in the horn fly (Diptera: Muscidae)
    Felix D Guerrero
    USDA ARS Knipling Bushland U S Livestock Insects Research Laboratory, 2700 Fredericksburg Rd, Kerrville, TX 78028, USA
    J Med Entomol 46:257-70. 2009
    ....
  39. ncbi request reprint Detection of protozoan parasites and microsporidia in irrigation waters used for crop production
    Jeanette A Thurston-Enriquez
    US Department of Agriculture, Agricultural Research Service, University of Nebraska, Lincoln 68583 0934, USA
    J Food Prot 65:378-82. 2002
    ..The presence of human pathogenic parasites in irrigation waters used in the production of crops traditionally consumed raw suggests that there may be a risk of infection to consumers who come in contact with or eat these products...
  40. doi request reprint Evaluation of the bacterial diversity in cecal contents of laying hens fed various molting diets by using bacterial tag-encoded FLX amplicon pyrosequencing
    T R Callaway
    Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service, USDA, College Station, TX 77845, USA
    Poult Sci 88:298-302. 2009
    ..54 genera, respectively). Overall, the microbial diversity was least and Lactobacillius populations were not found in the hens undergoing feed withdrawal, which could explain much of these hens' sensitivity to colonization by Salmonella...
  41. doi request reprint A soil bacterium regulates plant acquisition of iron via deficiency-inducible mechanisms
    Huiming Zhang
    Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, 79409, USA
    Plant J 58:568-77. 2009
    ..These results demonstrate the potential of microbes to control iron acquisition in plants and emphasize the sophisticated integration of microbial signaling in photosynthetic regulation...
  42. doi request reprint Intestinal aganglionosis is associated with early and sustained disruption of the colonic microbiome
    N L Ward
    Department of Molecular Biology, University of Wyoming, Laramie, WY, USA
    Neurogastroenterol Motil 24:874-e400. 2012
    ..We hypothesized that aganglionosis leads to microbiome changes that may contribute to HAEC risk...
  43. ncbi request reprint A database of expressed genes from Cochliomyia hominivorax (Diptera: Calliphoridae)
    F D Guerrero
    USDA ARS, Knipling Bushland U S Livestock Insects Research Laboratory 2700 Fredericksburg Rd, Kerrville, TX 78028, USA
    J Med Entomol 46:1109-16. 2009
    ..This database will facilitate the design of microarray and other experiments to study screwworm gene expression on a larger scale than previously possible...
  44. ncbi request reprint Biofilms and chronic wound inflammation
    R D Wolcott
    Southwest Regional Wound Care Center, Lubbock, Texas, USA
    J Wound Care 17:333-41. 2008
    ..In contrast to the commonly accepted hypothesis of host-centred pathology, it is possible that surface bacteria, not host dysfunction, cause the chronicity and perpetual inflammation associated with chronic non-healing wounds...
  45. doi request reprint In vitro multispecies Lubbock chronic wound biofilm model
    Yan Sun
    Medical Biofilm Research Institute, Lubbock, Texas 79410, USA
    Wound Repair Regen 16:805-13. 2008
    ..We have defined a realistic in vitro multispecies biofilm model simulating the functional characteristics of chronic pathogenic biofilms and developed effective tools for its characterization and analyses...
  46. ncbi request reprint Confirmed detection of Cyclospora cayetanesis, Encephalitozoon intestinalis and Cryptosporidium parvum in water used for drinking
    Scot E Dowd
    United States Department of Agriculture, Agricultural Research Service, Livestock Issues Unit, Texas Technological University, Lubbock, TX 79409, USA
    J Water Health 1:117-23. 2003
    ..The fact that the waters tested may be used for human consumption indicates that these emerging protozoa may be transmitted by ingestion of contaminated water...
  47. doi request reprint Soil bacteria confer plant salt tolerance by tissue-specific regulation of the sodium transporter HKT1
    Huiming Zhang
    Texas Tech University, Department of Chemistry, Biochemistry and Biology Lubbock 79409, U S A
    Mol Plant Microbe Interact 21:737-44. 2008
    ..These results demonstrate that tissue-specific regulation of HKT1 is critical for managing Na(+) homeostasis in salt-stressed plants, as well as underscore the breadth and sophistication of plant-microbe interactions...
  48. ncbi request reprint Differential expression of virulence-related genes in A Salmonella enterica serotype typhimurium luxS mutant in response to autoinducer AI-2 and poultry meat-derived AI-2 inhibitor
    Kenneth W Widmer
    Food Safety and Environmental Microbiology Program, Departments of Poultry Science and Nutrition and Food Science, Texas A and M University, College Station, TX 77843, USA
    Foodborne Pathog Dis 4:5-15. 2007
    ..Understanding the interaction of AI-2 and inhibitors of AI-2 activity found in poultry meat can help explain Salmonella survival and virulence on poultry products...
  49. ncbi request reprint Frequency-dependent response of the vascular endothelium to pulsatile shear stress
    Heather A Himburg
    Department of Biomedical Engineering, Duke University Durham, NC 27708, USA
    Am J Physiol Heart Circ Physiol 293:H645-53. 2007
    ..This study suggests that arterial regions subject to both shear reversal and dominant frequencies that exceed the normal heart rate are at greater risk for atherosclerotic lesion development...