B S Dien

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. ncbi request reprint Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol
    Bruce S Dien
    National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, United States
    Bioresour Technol 99:5216-25. 2008
  2. ncbi request reprint Fermentation of "Quick Fiber" produced from a modified corn-milling process into ethanol and recovery of corn fiber
    Bruce S Dien
    National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
    Appl Biochem Biotechnol 113:937-49. 2004
  3. ncbi request reprint Bacteria engineered for fuel ethanol production: current status
    B S Dien
    National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA
    Appl Microbiol Biotechnol 63:258-66. 2003
  4. doi request reprint Genetically engineered Escherichia coli FBR5: part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose
    N Qureshi
    United States Department of Agriculture, ARS, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA
    Biotechnol Prog 28:1167-78. 2012
  5. ncbi request reprint Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomass
    B S Dien
    Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA
    Appl Biochem Biotechnol 84:181-96. 2000
  6. ncbi request reprint Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars
    B S Dien
    Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 27:259-64. 2001
  7. doi request reprint Genetically engineered Escherichia coli FBR5: part II. Ethanol production from xylose and simultaneous product recovery
    N Qureshi
    United States Dept of Agriculture, ARS, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA
    Biotechnol Prog 28:1179-85. 2012
  8. ncbi request reprint Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPE1, and TKL1 in Saccharomyces cerevisiae
    S W Gorsich
    National Center for Agriculture Utilization Research, Agriculture Research Service, USDA, Peoria, IL, USA
    Appl Microbiol Biotechnol 71:339-49. 2006
  9. ncbi request reprint Use of catabolite repression mutants for fermentation of sugar mixtures to ethanol
    N N Nichols
    National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, Peoria, IL 61604, USA
    Appl Microbiol Biotechnol 56:120-5. 2001
  10. ncbi request reprint Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2,5-bis-hydroxymethylfuran
    Z L Liu
    Crop Bioprotection, Fermentation Biotechnology, Microbial Genomics and Bioprocessing, and New Crops and Processing Technology Research Units, National Center for Agricultural Utilization Research, USDA ARS, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 31:345-52. 2004

Detail Information

Publications27

  1. ncbi request reprint Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol
    Bruce S Dien
    National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, United States
    Bioresour Technol 99:5216-25. 2008
    ..Pretreated DDGS were readily fermented and were converted to ethanol at 89-90% efficiency based upon total glucans; S. cerevisiae does not ferment arabinose or xylose...
  2. ncbi request reprint Fermentation of "Quick Fiber" produced from a modified corn-milling process into ethanol and recovery of corn fiber
    Bruce S Dien
    National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
    Appl Biochem Biotechnol 113:937-49. 2004
    ..04 lb/bu. The residuals from the fermentations were also evaluated as a source of corn fiber oil, which has value as a nutraceutical. Corn fiber oil yields were 8.28%wt for solids recovered following pretreatment...
  3. ncbi request reprint Bacteria engineered for fuel ethanol production: current status
    B S Dien
    National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA
    Appl Microbiol Biotechnol 63:258-66. 2003
    ..Work on this organism has concentrated on introducing pathways for the fermentation of arabinose and xylose. The history of constructing these strains and current progress in refining them are detailed in this review...
  4. doi request reprint Genetically engineered Escherichia coli FBR5: part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose
    N Qureshi
    United States Department of Agriculture, ARS, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA
    Biotechnol Prog 28:1167-78. 2012
    ..04 gL(-1) h(-1) whereas adsorbed cell packed bed reactor resulted in a productivity of 4.39 gL(-1) h(-1). In the five reactor systems, ethanol concentrations ranged from 18.9 to 40.30 gL(-1) with metabolic yields from 0.44 to 0.51...
  5. ncbi request reprint Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomass
    B S Dien
    Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA
    Appl Biochem Biotechnol 84:181-96. 2000
    ..4% (w/v) of the available sugar, whereas strain FBR4 left 1.6% unconsumed. The fermentation with FBR5 was completed within 55 h and yielded 0.46 g of ethanol/g of available sugar, 90% of the maximum obtainable...
  6. ncbi request reprint Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars
    B S Dien
    Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 27:259-64. 2001
    ..FBR11 exhausted the glucose within 30 h, and the maximum lactic acid concentration (7.32% w/v) was 93% of the theoretical maximum. The other side-products detected were cell mass and succinic acid (0.5 g/l)...
  7. doi request reprint Genetically engineered Escherichia coli FBR5: part II. Ethanol production from xylose and simultaneous product recovery
    N Qureshi
    United States Dept of Agriculture, ARS, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA
    Biotechnol Prog 28:1179-85. 2012
    ..41 g L(-1). This process has potential for greatly improving the performance of E. coli FBR5 where the strain can ferment all the lignocellulosic sugars to ethanol and gas stripping can be applied to recover product...
  8. ncbi request reprint Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPE1, and TKL1 in Saccharomyces cerevisiae
    S W Gorsich
    National Center for Agriculture Utilization Research, Agriculture Research Service, USDA, Peoria, IL, USA
    Appl Microbiol Biotechnol 71:339-49. 2006
    ..These results demonstrate a strong relationship between PPP genes and furfural tolerance and provide additional putative target genes involved in furfural tolerance...
  9. ncbi request reprint Use of catabolite repression mutants for fermentation of sugar mixtures to ethanol
    N N Nichols
    National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, Peoria, IL 61604, USA
    Appl Microbiol Biotechnol 56:120-5. 2001
    ..Based upon fermentations of sugar mixtures, a catabolite-repression mutant of ethanologenic E. coli is expected to provide more efficient fermentation of hemicellulose hydrolysates by allowing direct utilization of pentoses...
  10. ncbi request reprint Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2,5-bis-hydroxymethylfuran
    Z L Liu
    Crop Bioprotection, Fermentation Biotechnology, Microbial Genomics and Bioprocessing, and New Crops and Processing Technology Research Units, National Center for Agricultural Utilization Research, USDA ARS, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 31:345-52. 2004
    ..The ability of yeasts to adapt to and transform furfural and HMF offers the potential for in situ detoxification of these inhibitors and suggests a genetic basis for further development of highly tolerant strains for biofuel production...
  11. ncbi request reprint Engineering lactic acid bacteria with pyruvate decarboxylase and alcohol dehydrogenase genes for ethanol production from Zymomonas mobilis
    Nancy N Nichols
    Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 30:315-21. 2003
    ..However, lactic acid was the primary fermentation product formed by all of the transformants, indicating that ADH and PDC activities were insufficient to divert significant carbon flow towards ethanol...
  12. ncbi request reprint Butanol production by Clostridium beijerinckii. Part I: use of acid and enzyme hydrolyzed corn fiber
    Nasib Qureshi
    United States Department of Agriculture 1, National Center for Agricultural Utilization Research, Fermentation Biotechnology, 1815 N University Street, Peoria, IL 61604, USA
    Bioresour Technol 99:5915-22. 2008
    ..beijerinckii BA101. In our fermentations, an ABE yield ranging from 0.35 to 0.39 was obtained, which is higher than reported by the other investigators...
  13. doi request reprint Assessment of bermudagrass and bunch grasses as feedstock for conversion to ethanol
    William F Anderson
    Coastal Plain Experiment Station, ARS USDA, Tifton, GA 31793, USA
    Appl Biochem Biotechnol 145:13-21. 2008
    ..Results suggest that ethanol yields vary significantly for feedstocks by species and within species and that genetic breeding for improved feedstocks should be possible...
  14. doi request reprint Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system
    Stephen R Hughes
    United States Department of Agriculture USDA, Agricultural Research Service ARS, National Center for Agricultural Utilization Research NCAUR, Peoria, IL 61604, USA
    Plasmid 61:22-38. 2009
    ..Yeast strains expressing XI and xylulokinase with or without Lyt-1 showed improved growth on xylose compared to INVSc1-XI yeast...
  15. doi request reprint Fermentation of bioenergy crops into ethanol using biological abatement for removal of inhibitors
    Nancy N Nichols
    Fermentation Biotechnology Research Unit, NCAUR, USDA ARS, Peoria, IL 61604, USA
    Bioresour Technol 101:7545-50. 2010
    ..Bioabatement was as effective as lime conditioning at reducing fermentation lag times. Prolonged incubations with the bioabatement microbe resulted in consumption of some glucose and reduced production of ethanol...
  16. doi request reprint Selective chemical oxidation and depolymerization of switchgrass [corrected] (Panicum virgatum L.) xylan with [corrected] oligosaccharide product analysis by mass spectrometry
    Michael J Bowman
    USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 North University Street, Peoria, IL 61604, USA
    Rapid Commun Mass Spectrom 25:941-50. 2011
    ....
  17. ncbi request reprint Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis
    Siqing Liu
    Bioproducts and Biocatalysis Research Unit, Peoria, IL, USA
    FEMS Microbiol Lett 274:291-7. 2007
    ..This work provides evidence that expressing Gram-positive ethanol genes in pentose utilizing L. brevis will further aid manipulation of this microbe toward biomass to ethanol production...
  18. ncbi request reprint Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina
    Xin Liang Li
    Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, United States Department of Agriculture Agricultural Research Service, 1815 N University Street, Peoria, IL, USA
    Appl Microbiol Biotechnol 74:1264-75. 2007
    ..The work demonstrated for the first time that glycoside hydrolases derived from anaerobic fungi can be produced by H. jecorina...
  19. ncbi request reprint Properties of a recombinant beta-glucosidase from polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysis
    Xin Liang Li
    Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA ARS, 1815 N University Street, Peoria, IL 61604 3902, USA
    Appl Biochem Biotechnol 113:233-50. 2004
    ..6 mM. Beta-glucosidase significantly enhanced the conversion of cellulosic materials into glucose by Trichoderma reesei cellulase preparations, demonstrating its potential for use in biofuel and feedstock chemical production...
  20. ncbi request reprint Profile of enzyme production by Trichoderma reesei grown on corn fiber fractions
    Xin Liang Li
    Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA ARS, 1815 N University Street, Peoria, Illinois 61604
    Appl Biochem Biotechnol 121:321-34. 2005
    ..T. reesei QM9414 and Rut C-30 grew on CCF, DSCF, CFC, or CFAX and secreted a number of hydrolytic enzymes. The enzymes displayed synergism with commercial cellulases for corn fiber hydrolysis...
  21. ncbi request reprint Bioabatement to remove inhibitors from biomass-derived sugar hydrolysates
    Nancy N Nichols
    Fermentation Biotechnology Research Unit, NCAUR, USDA ARS, 1815 N University Street, Peoria, IL 61604, USA
    Appl Biochem Biotechnol 121:379-90. 2005
    ..NRRL30616 was the best strain for removal of inhibitors from hydrolysate, and abatement of hydrolysate by inoculation with the strain allowed subsequent yeast fermentation of cellulose to ethanol...
  22. ncbi request reprint Functional expression of bacterial Zymobacter palmae pyruvate decarboxylase gene in Lactococcus lactis
    Siqing Liu
    Bioproducts and Biocatalysis Research Unit, National Center for Agriculture Utilization Research, USDA, ARS, 1815 N University St, Peoria, IL, 61604, USA
    Curr Microbiol 50:324-8. 2005
    ..However, no detectable differences for ethanol fermentation in these engineered strains were observed compared with that of the strain carrying lacZ reporter...
  23. ncbi request reprint Nitrogen source and mineral optimization enhance D: -xylose conversion to ethanol by the yeast Pichia stipitis NRRL Y-7124
    Patricia J Slininger
    U S Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N University Street, Peoria, IL 61604, USA
    Appl Microbiol Biotechnol 72:1285-96. 2006
    ..In nongrowing cultures supplied with nitrogen as amino acids, ethanol concentration increased from 24 to 54 g/l with the addition of an optimized mineral supplement of Fe, Mn, Mg, Ca, Zn, and others...
  24. ncbi request reprint Metabolic engineering of a Lactobacillus plantarum double ldh knockout strain for enhanced ethanol production
    Siqing Liu
    Bioproducts and Biocatalysis Research Unit, National Center for Agriculture Utilization Research, USDA, ARS, 1815 N University St, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 33:1-7. 2006
    ..Although slightly more ethanol was observed, carbon flow was not significantly improved toward ethanol, suggesting that a further understanding of this organism's metabolism is necessary...
  25. ncbi request reprint Fermentation of sugar mixtures using Escherichia coli catabolite repression mutants engineered for production of L-lactic acid
    B S Dien
    Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
    J Ind Microbiol Biotechnol 29:221-7. 2002
    ..Therefore, higher expression of xylose metabolic genes in the ptsG(-) strain may be responsible for superior conversion of xylose to product compared to the ptsG(+) fermentations...
  26. doi request reprint Enzyme production by industrially relevant fungi cultured on coproduct from corn dry grind ethanol plants
    Eduardo A Ximenes
    Microbiology Department, University of Georgia, 204 Biological Sciences, Athens, GA 30602 2605, USA
    Appl Biochem Biotechnol 137:171-83. 2007
    ..reesei) or updraft marine impellers (A. niger). Initial production titers were lower for bioreactor than for flask cultures, especially for T. reesei cultures. Improvement of enzyme titers were obtained using fed-batch feeding schemes...
  27. ncbi request reprint Industrial scale-up of pH-controlled liquid hot water pretreatment of corn fiber for fuel ethanol production
    Nathan S Mosier
    Laboratory of Renewable Resources Engineering, Department of Agricultural and Biological Engineering, Purdue University, 500 Central Drive, West Lafayette, IN 47907 2022, USA
    Appl Biochem Biotechnol 125:77-97. 2005
    ....