Research Topics
| B S DienSummaryAffiliation: Agricultural Research Service Country: USA Publications
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Detail Information
Publications
Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanolBruce S Dien
National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, United States
Bioresour Technol 99:5216-25. 2008..Pretreated DDGS were readily fermented and were converted to ethanol at 89-90% efficiency based upon total glucans; S. cerevisiae does not ferment arabinose or xylose...- Fermentation of "Quick Fiber" produced from a modified corn-milling process into ethanol and recovery of corn fiberBruce S Dien
National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
Appl Biochem Biotechnol 113:937-49. 2004..04 lb/bu. The residuals from the fermentations were also evaluated as a source of corn fiber oil, which has value as a nutraceutical. Corn fiber oil yields were 8.28%wt for solids recovered following pretreatment... - Bacteria engineered for fuel ethanol production: current statusB S Dien
National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA
Appl Microbiol Biotechnol 63:258-66. 2003..Work on this organism has concentrated on introducing pathways for the fermentation of arabinose and xylose. The history of constructing these strains and current progress in refining them are detailed in this review... 
Genetically engineered Escherichia coli FBR5: part I. Comparison of high cell density bioreactors for enhanced ethanol production from xyloseN Qureshi
United States Department of Agriculture, ARS, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA
Biotechnol Prog 28:1167-78. 2012..04 gL(-1) h(-1) whereas adsorbed cell packed bed reactor resulted in a productivity of 4.39 gL(-1) h(-1). In the five reactor systems, ethanol concentrations ranged from 18.9 to 40.30 gL(-1) with metabolic yields from 0.44 to 0.51...- Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomassB S Dien
Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA
Appl Biochem Biotechnol 84:181-96. 2000..4% (w/v) of the available sugar, whereas strain FBR4 left 1.6% unconsumed. The fermentation with FBR5 was completed within 55 h and yielded 0.46 g of ethanol/g of available sugar, 90% of the maximum obtainable... - Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugarsB S Dien
Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
J Ind Microbiol Biotechnol 27:259-64. 2001..FBR11 exhausted the glucose within 30 h, and the maximum lactic acid concentration (7.32% w/v) was 93% of the theoretical maximum. The other side-products detected were cell mass and succinic acid (0.5 g/l)... - Genetically engineered Escherichia coli FBR5: part II. Ethanol production from xylose and simultaneous product recoveryN Qureshi
United States Dept of Agriculture, ARS, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 N University Street, Peoria, IL 61604, USA
Biotechnol Prog 28:1179-85. 2012..41 g L(-1). This process has potential for greatly improving the performance of E. coli FBR5 where the strain can ferment all the lignocellulosic sugars to ethanol and gas stripping can be applied to recover product... - Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPE1, and TKL1 in Saccharomyces cerevisiaeS W Gorsich
National Center for Agriculture Utilization Research, Agriculture Research Service, USDA, Peoria, IL, USA
Appl Microbiol Biotechnol 71:339-49. 2006..These results demonstrate a strong relationship between PPP genes and furfural tolerance and provide additional putative target genes involved in furfural tolerance... - Use of catabolite repression mutants for fermentation of sugar mixtures to ethanolN N Nichols
National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, Peoria, IL 61604, USA
Appl Microbiol Biotechnol 56:120-5. 2001..Based upon fermentations of sugar mixtures, a catabolite-repression mutant of ethanologenic E. coli is expected to provide more efficient fermentation of hemicellulose hydrolysates by allowing direct utilization of pentoses... - Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2,5-bis-hydroxymethylfuranZ L Liu
Crop Bioprotection, Fermentation Biotechnology, Microbial Genomics and Bioprocessing, and New Crops and Processing Technology Research Units, National Center for Agricultural Utilization Research, USDA ARS, Peoria, IL 61604, USA
J Ind Microbiol Biotechnol 31:345-52. 2004..The ability of yeasts to adapt to and transform furfural and HMF offers the potential for in situ detoxification of these inhibitors and suggests a genetic basis for further development of highly tolerant strains for biofuel production... - Engineering lactic acid bacteria with pyruvate decarboxylase and alcohol dehydrogenase genes for ethanol production from Zymomonas mobilisNancy N Nichols
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA
J Ind Microbiol Biotechnol 30:315-21. 2003..However, lactic acid was the primary fermentation product formed by all of the transformants, indicating that ADH and PDC activities were insufficient to divert significant carbon flow towards ethanol... - Butanol production by Clostridium beijerinckii. Part I: use of acid and enzyme hydrolyzed corn fiberNasib Qureshi
United States Department of Agriculture 1, National Center for Agricultural Utilization Research, Fermentation Biotechnology, 1815 N University Street, Peoria, IL 61604, USA
Bioresour Technol 99:5915-22. 2008..beijerinckii BA101. In our fermentations, an ABE yield ranging from 0.35 to 0.39 was obtained, which is higher than reported by the other investigators... - Assessment of bermudagrass and bunch grasses as feedstock for conversion to ethanolWilliam F Anderson
Coastal Plain Experiment Station, ARS USDA, Tifton, GA 31793, USA
Appl Biochem Biotechnol 145:13-21. 2008..Results suggest that ethanol yields vary significantly for feedstocks by species and within species and that genetic breeding for improved feedstocks should be possible... - Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression systemStephen R Hughes
United States Department of Agriculture USDA, Agricultural Research Service ARS, National Center for Agricultural Utilization Research NCAUR, Peoria, IL 61604, USA
Plasmid 61:22-38. 2009..Yeast strains expressing XI and xylulokinase with or without Lyt-1 showed improved growth on xylose compared to INVSc1-XI yeast... - Fermentation of bioenergy crops into ethanol using biological abatement for removal of inhibitorsNancy N Nichols
Fermentation Biotechnology Research Unit, NCAUR, USDA ARS, Peoria, IL 61604, USA
Bioresour Technol 101:7545-50. 2010..Bioabatement was as effective as lime conditioning at reducing fermentation lag times. Prolonged incubations with the bioabatement microbe resulted in consumption of some glucose and reduced production of ethanol... - Selective chemical oxidation and depolymerization of switchgrass [corrected] (Panicum virgatum L.) xylan with [corrected] oligosaccharide product analysis by mass spectrometryMichael J Bowman
USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, Bioenergy Research Unit, 1815 North University Street, Peoria, IL 61604, USA
Rapid Commun Mass Spectrom 25:941-50. 2011.... - Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevisSiqing Liu
Bioproducts and Biocatalysis Research Unit, Peoria, IL, USA
FEMS Microbiol Lett 274:291-7. 2007..This work provides evidence that expressing Gram-positive ethanol genes in pentose utilizing L. brevis will further aid manipulation of this microbe toward biomass to ethanol production... - Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorinaXin Liang Li
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, United States Department of Agriculture Agricultural Research Service, 1815 N University Street, Peoria, IL, USA
Appl Microbiol Biotechnol 74:1264-75. 2007..The work demonstrated for the first time that glycoside hydrolases derived from anaerobic fungi can be produced by H. jecorina... - Properties of a recombinant beta-glucosidase from polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysisXin Liang Li
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA ARS, 1815 N University Street, Peoria, IL 61604 3902, USA
Appl Biochem Biotechnol 113:233-50. 2004..6 mM. Beta-glucosidase significantly enhanced the conversion of cellulosic materials into glucose by Trichoderma reesei cellulase preparations, demonstrating its potential for use in biofuel and feedstock chemical production... - Profile of enzyme production by Trichoderma reesei grown on corn fiber fractionsXin Liang Li
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA ARS, 1815 N University Street, Peoria, Illinois 61604
Appl Biochem Biotechnol 121:321-34. 2005..T. reesei QM9414 and Rut C-30 grew on CCF, DSCF, CFC, or CFAX and secreted a number of hydrolytic enzymes. The enzymes displayed synergism with commercial cellulases for corn fiber hydrolysis... - Bioabatement to remove inhibitors from biomass-derived sugar hydrolysatesNancy N Nichols
Fermentation Biotechnology Research Unit, NCAUR, USDA ARS, 1815 N University Street, Peoria, IL 61604, USA
Appl Biochem Biotechnol 121:379-90. 2005..NRRL30616 was the best strain for removal of inhibitors from hydrolysate, and abatement of hydrolysate by inoculation with the strain allowed subsequent yeast fermentation of cellulose to ethanol... - Functional expression of bacterial Zymobacter palmae pyruvate decarboxylase gene in Lactococcus lactisSiqing Liu
Bioproducts and Biocatalysis Research Unit, National Center for Agriculture Utilization Research, USDA, ARS, 1815 N University St, Peoria, IL, 61604, USA
Curr Microbiol 50:324-8. 2005..However, no detectable differences for ethanol fermentation in these engineered strains were observed compared with that of the strain carrying lacZ reporter... - Nitrogen source and mineral optimization enhance D: -xylose conversion to ethanol by the yeast Pichia stipitis NRRL Y-7124Patricia J Slininger
U S Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N University Street, Peoria, IL 61604, USA
Appl Microbiol Biotechnol 72:1285-96. 2006..In nongrowing cultures supplied with nitrogen as amino acids, ethanol concentration increased from 24 to 54 g/l with the addition of an optimized mineral supplement of Fe, Mn, Mg, Ca, Zn, and others... - Metabolic engineering of a Lactobacillus plantarum double ldh knockout strain for enhanced ethanol productionSiqing Liu
Bioproducts and Biocatalysis Research Unit, National Center for Agriculture Utilization Research, USDA, ARS, 1815 N University St, Peoria, IL 61604, USA
J Ind Microbiol Biotechnol 33:1-7. 2006..Although slightly more ethanol was observed, carbon flow was not significantly improved toward ethanol, suggesting that a further understanding of this organism's metabolism is necessary... - Fermentation of sugar mixtures using Escherichia coli catabolite repression mutants engineered for production of L-lactic acidB S Dien
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA
J Ind Microbiol Biotechnol 29:221-7. 2002..Therefore, higher expression of xylose metabolic genes in the ptsG(-) strain may be responsible for superior conversion of xylose to product compared to the ptsG(+) fermentations... - Enzyme production by industrially relevant fungi cultured on coproduct from corn dry grind ethanol plantsEduardo A Ximenes
Microbiology Department, University of Georgia, 204 Biological Sciences, Athens, GA 30602 2605, USA
Appl Biochem Biotechnol 137:171-83. 2007..reesei) or updraft marine impellers (A. niger). Initial production titers were lower for bioreactor than for flask cultures, especially for T. reesei cultures. Improvement of enzyme titers were obtained using fed-batch feeding schemes... - Industrial scale-up of pH-controlled liquid hot water pretreatment of corn fiber for fuel ethanol productionNathan S Mosier
Laboratory of Renewable Resources Engineering, Department of Agricultural and Biological Engineering, Purdue University, 500 Central Drive, West Lafayette, IN 47907-2022, USA
Appl Biochem Biotechnol 125:77-97. 2005....