Perng Kuang Chang


Affiliation: Agricultural Research Service
Country: USA


  1. Hua S, Sarreal S, Chang P, Yu J. Transcriptional Regulation of Aflatoxin Biosynthesis and Conidiation in Aspergillus flavus by Wickerhamomyces anomalus WRL-076 for Reduction of Aflatoxin Contamination. Toxins (Basel). 2019;11: pubmed publisher
    ..Furthermore, fungal biomass and conidial number were significantly reduced by 60% and more than 95%, respectively. The results validate the biocontrol efficacy of W. anomalus WRL-076 observed in the field experiments. ..
  2. Chang P, Zhang Q, Scharfenstein L, Mack B, Yoshimi A, Miyazawa K, et al. Aspergillus flavus GPI-anchored protein-encoding ecm33 has a role in growth, development, aflatoxin biosynthesis, and maize infection. Appl Microbiol Biotechnol. 2018;102:5209-5220 pubmed publisher
    ..A. flavus Ecm33 is required for proper cell wall composition and plays an important role in normal fungal growth and development, aflatoxin biosynthesis, and seed colonization. ..
  3. Chang P, Hua S, Sarreal S, Li R. Suppression of Aflatoxin Biosynthesis in Aspergillus flavus by 2-Phenylethanol Is Associated with Stimulated Growth and Decreased Degradation of Branched-Chain Amino Acids. Toxins (Basel). 2015;7:3887-902 pubmed publisher
    ..Metabolic flux in primary metabolism apparently has an important role in the expression of genes of secondary metabolism. ..
  4. Chang P, Scharfenstein L, Solorzano C, Abbas H, Hua S, Jones W, et al. High sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of Aspergillus oryzae. Int J Food Microbiol. 2015;200:66-71 pubmed publisher
    ..The results suggested that A. oryzae strains in the current culture collections were mostly derived from the samA-rosA/RIB40 lineage of the nonaflatoxigenic A. flavus with the S genotype. ..
  5. Chang P, Scharfenstein L, Li P, Ehrlich K. Aspergillus flavus VelB acts distinctly from VeA in conidiation and may coordinate with FluG to modulate sclerotial production. Fungal Genet Biol. 2013;58-59:71-9 pubmed publisher
    ..Concerted interactions of A. flavus VeA and VelB with LaeA are critical for conidiation and aflatoxin biosynthesis. VelB may have a dual role and likely coordinates with FluG to modulate sclerotial production. ..
  6. Li P, Zhou Q, Wang T, Zhou H, Zhang W, Ding X, et al. Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins. Toxins (Basel). 2015;8: pubmed publisher
    ..Our results showed that the monoclonal antibody 2 G6-based CI-ELISA was suitable for the detection of G-group aflatoxins present in peanuts and also those produced by fungi. ..
  7. Chang P, Ehrlich K. Genome-wide analysis of the Zn(II)?Cys? zinc cluster-encoding gene family in Aspergillus flavus. Appl Microbiol Biotechnol. 2013;97:4289-300 pubmed publisher
    ..The features revealed by the A. flavus C6 proteins likely are common for other ascomycete fungi. ..
  8. Chang P, Scharfenstein L, Mack B, Ehrlich K. Deletion of the Aspergillus flavus orthologue of A. nidulans fluG reduces conidiation and promotes production of sclerotia but does not abolish aflatoxin biosynthesis. Appl Environ Microbiol. 2012;78:7557-63 pubmed publisher
    ..flavus. These results suggest that the function of fluG and the signaling pathways related to conidiation are different in the two related aspergilli. ..
  9. Chang P, Abbas H, Weaver M, Ehrlich K, Scharfenstein L, Cotty P. Identification of genetic defects in the atoxigenic biocontrol strain Aspergillus flavus K49 reveals the presence of a competitive recombinant group in field populations. Int J Food Microbiol. 2012;154:192-6 pubmed publisher
    ..This finding provides an easy way to select future biocontrol strains from the reservoir of non-aflatoxigenic populations in agricultural fields. ..

More Information


  1. Chang P, Scharfenstein L, Ehrlich K, Wei Q, Bhatnagar D, Ingber B. Effects of laeA deletion on Aspergillus flavus conidial development and hydrophobicity may contribute to loss of aflatoxin production. Fungal Biol. 2012;116:298-307 pubmed publisher
    ..The loss of hydrophobicity and the other developmental changes in the laeA deletion mutants could affect the ability of the fungus to produce aflatoxins...
  2. Chang P, Scharfenstein L, Wei Q, Bhatnagar D. Development and refinement of a high-efficiency gene-targeting system for Aspergillus flavus. J Microbiol Methods. 2010;81:240-6 pubmed publisher
    ..The improvement allowed gene manipulation using the reusable pyrG marker as shown by the restoration of laeA-mediated aflatoxin production in an A. flavus laeA-deleted mutant. ..
  3. Chang P, Horn B, Dorner J. Clustered genes involved in cyclopiazonic acid production are next to the aflatoxin biosynthesis gene cluster in Aspergillus flavus. Fungal Genet Biol. 2009;46:176-82 pubmed publisher
    ..flavus strain. Therefore, some of the CPA biosynthesis genes are organized in a mini-gene cluster that is next to the aflatoxin gene cluster in A. flavus...
  4. Chang P, Scharfenstein L, Luo M, Mahoney N, Molyneux R, Yu J, et al. Loss of msnA, a putative stress regulatory gene, in Aspergillus parasiticus and Aspergillus flavus increased production of conidia, aflatoxins and kojic acid. Toxins (Basel). 2011;3:82-104 pubmed publisher
    ..flavus. The msnA gene appears to be required for the maintenance of the normal oxidative state. The impairment of msnA resulted in the aforementioned changes, which might be used to combat the increased oxidative stress in the cells. ..
  5. Chang P, Scharfenstein L, Ehrlich K, Diana Di Mavungu J. The Aspergillus flavus fluP-associated metabolite promotes sclerotial production. Fungal Biol. 2016;120:1258-68 pubmed publisher
    ..These results suggest that FluP polyketide synthase is involved in the synthesis of a diffusible metabolite that could serve as a signal molecule to regulate sclerotiogenesis. ..
  6. Chang P, Scharfenstein L, Li R, Arroyo Manzanares N, De Saeger S, Diana Di Mavungu J. Aspergillus flavus aswA, a gene homolog of Aspergillus nidulans oefC, regulates sclerotial development and biosynthesis of sclerotium-associated secondary metabolites. Fungal Genet Biol. 2017;104:29-37 pubmed publisher
    ..Impairment in aswA gene function stalls normal sclerotial development, which in turn prevents biosynthesis and accumulation of sclerotium-specific metabolites. ..
  7. Cary J, Harris Coward P, Scharfenstein L, Mack B, Chang P, Wei Q, et al. The Aspergillus flavus Homeobox Gene, hbx1, is Required for Development and Aflatoxin Production. Toxins (Basel). 2017;9: pubmed publisher
    ..Our results demonstrate that hbx1 not only plays a significant role in controlling A. flavus development but is also critical for the production of secondary metabolites, such as aflatoxins. ..
  8. Chang P, Ehrlich K. What does genetic diversity of Aspergillus flavus tell us about Aspergillus oryzae?. Int J Food Microbiol. 2010;138:189-99 pubmed publisher
    ..flavus and A. oryzae genomes, the genetic diversity in A. flavus and A. oryzae populations, the causes of this diversity, and the relatedness of nonaflatoxigenic A. flavus strains to A. oryzae...