Heping Cao

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. pmc Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis
    Heping Cao
    Laboratories of Neurobiology and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Biochem J 394:285-97. 2006
  2. pmc Expression, purification, and biochemical characterization of the antiinflammatory tristetraprolin: a zinc-dependent mRNA binding protein affected by posttranslational modifications
    Heping Cao
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 43:13724-38. 2004
  3. pmc Immunological characterization of tristetraprolin as a low abundance, inducible, stable cytosolic protein
    Heping Cao
    Laboratory of Signal Transduction and Office of Clinical Research, National Institute of Environmental Health Sciences, NIH DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:21489-99. 2004
  4. doi request reprint Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae
    Jay Shockey
    United States Department of Agriculture Agricultural Research Service, Southern Regional Research Center, New Orleans, LA 70124, USA
    Appl Microbiol Biotechnol 92:1207-17. 2011
  5. pmc Structure-function analysis of diacylglycerol acyltransferase sequences from 70 organisms
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, U, S, Department of Agriculture, 1100 Robert E, Lee Blvd, New Orleans, Louisiana 70124, USA
    BMC Res Notes 4:249. 2011
  6. pmc Expression of tung tree diacylglycerol acyltransferase 1 in E. coli
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 1100 Robert E, Lee Blvd, New Orleans, LA 70124, USA
    BMC Biotechnol 11:73. 2011
  7. doi request reprint Cinnamon polyphenol extract regulates tristetraprolin and related gene expression in mouse adipocytes
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 1100 Robert E Lee Boulevard, New Orleans, Louisiana 70124, United States
    J Agric Food Chem 59:2739-44. 2011
  8. doi request reprint Cinnamon extract regulates glucose transporter and insulin-signaling gene expression in mouse adipocytes
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 1100 Robert E Lee Blvd, New Orleans, LA 70124, USA
    Phytomedicine 17:1027-32. 2010
  9. ncbi request reprint Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    J Nutr 138:833-40. 2008
  10. doi request reprint Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland, USA
    Obesity (Silver Spring) 16:1208-18. 2008

Collaborators

Detail Information

Publications16

  1. pmc Identification of the anti-inflammatory protein tristetraprolin as a hyperphosphorylated protein by mass spectrometry and site-directed mutagenesis
    Heping Cao
    Laboratories of Neurobiology and Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA
    Biochem J 394:285-97. 2006
    ....
  2. pmc Expression, purification, and biochemical characterization of the antiinflammatory tristetraprolin: a zinc-dependent mRNA binding protein affected by posttranslational modifications
    Heping Cao
    Laboratory of Neurobiology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 43:13724-38. 2004
    ..The results support a model in which each subunit of the TTP tetramer binds to one of the five overlapping UUAUUUAUU sequences of the ARE, resulting in a stable TTP-ARE complex...
  3. pmc Immunological characterization of tristetraprolin as a low abundance, inducible, stable cytosolic protein
    Heping Cao
    Laboratory of Signal Transduction and Office of Clinical Research, National Institute of Environmental Health Sciences, NIH DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    J Biol Chem 279:21489-99. 2004
    ..TTP appears to be a low abundance, cytosolic protein in unstimulated cells and tissues, but once induced is relatively stable, in contrast to its very labile mRNA...
  4. doi request reprint Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae
    Jay Shockey
    United States Department of Agriculture Agricultural Research Service, Southern Regional Research Center, New Orleans, LA 70124, USA
    Appl Microbiol Biotechnol 92:1207-17. 2011
    ....
  5. pmc Structure-function analysis of diacylglycerol acyltransferase sequences from 70 organisms
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, U, S, Department of Agriculture, 1100 Robert E, Lee Blvd, New Orleans, Louisiana 70124, USA
    BMC Res Notes 4:249. 2011
    ..abstract:..
  6. pmc Expression of tung tree diacylglycerol acyltransferase 1 in E. coli
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 1100 Robert E, Lee Blvd, New Orleans, LA 70124, USA
    BMC Biotechnol 11:73. 2011
    ..coli had not been reported because DGAT1s are integral membrane proteins and difficult to express and purify. The objective of this study was to establish a procedure for expressing full-length DGAT1 in E. coli...
  7. doi request reprint Cinnamon polyphenol extract regulates tristetraprolin and related gene expression in mouse adipocytes
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, 1100 Robert E Lee Boulevard, New Orleans, Louisiana 70124, United States
    J Agric Food Chem 59:2739-44. 2011
    ..This study demonstrated that CPE rapidly induces TTP mRNA and reduces VEGF mRNA and affects the expression of a number of other genes in the cultured adipocytes...
  8. doi request reprint Cinnamon extract regulates glucose transporter and insulin-signaling gene expression in mouse adipocytes
    Heping Cao
    Commodity Utilization Research Unit, Southern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 1100 Robert E Lee Blvd, New Orleans, LA 70124, USA
    Phytomedicine 17:1027-32. 2010
    ..This study indicates that CE regulates the expression of multiple genes in adipocytes and this regulation could contribute to the potential health benefits of CE...
  9. ncbi request reprint Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    J Nutr 138:833-40. 2008
    ..These results suggest that CPE can affect immune responses by regulating anti- and proinflammatory and GLUT gene expression...
  10. doi request reprint Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes
    Heping Cao
    Diet, Genomics, and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland, USA
    Obesity (Silver Spring) 16:1208-18. 2008
    ..We analyzed the relative abundance of TTP family mRNAs in 3T3-L1 adipocytes compared to RAW264.7 macrophages and investigated insulin effects on the expression of 43 genes in 3T3-L1 adipocytes...
  11. pmc Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli
    Heping Cao
    Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, U S Department of Agriculture ARS, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Biotechnol Prog 24:326-33. 2008
    ..These results demonstrate that ZFP36L1 antiserum is useful in the detection of this protein and that TTP and ZFP36L1 are differentially expressed and regulated at the mRNA and protein levels in mouse adipocytes and macrophages...
  12. pmc Phosphorylation of recombinant tristetraprolin in vitro
    Heping Cao
    Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705, USA
    Protein J 27:163-9. 2008
    ..Autoradiography showed that TTP was phosphorylated in vitro by GSK3b, PKA, PKB, PKC, but not Cdc2, in addition to p42, p38, and JNK. These results demonstrate that TTP is a substrate for a number of protein kinases in vitro...
  13. pmc Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin
    Heping Cao
    US Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, 10300 Baltimore Avenue, Beltsville, MD 20705, USA
    Expert Rev Proteomics 4:711-26. 2007
    ..This information should provide the molecular basis for future investigation of TTP's regulatory functions in controlling proinflammatory cytokines...
  14. ncbi request reprint Green tea polyphenol extract regulates the expression of genes involved in glucose uptake and insulin signaling in rats fed a high fructose diet
    Heping Cao
    Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U S Department of Agriculture, Beltsville, Maryland 20705, USA
    J Agric Food Chem 55:6372-8. 2007
    ..This study shows that green tea extract at 1 or 2 g/kg diet regulates gene expression in the glucose uptake and insulin signaling pathway in rats fed a fructose-rich diet...
  15. ncbi request reprint Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin receptor, and glucose transporter 4 in mouse 3T3-L1 adipocytes
    Heping Cao
    Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705 2350, USA
    Arch Biochem Biophys 459:214-22. 2007
    ..These results suggest that cinnamon exhibits the potential to increase the amount of proteins involved in insulin signaling, glucose transport, and anti-inflammatory/anti-angiogenesis response...
  16. pmc Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases
    Heping Cao
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, Mail Drop F3 04, Research Triangle Park, NC 27709, USA
    Arch Biochem Biophys 412:106-20. 2003
    ....