Research Topics
| Arvind A BhagwatSummaryAffiliation: Agricultural Research Service Country: USA Publications
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Detail Information
Publications
Regulation of the glutamate-dependent acid-resistance system of diarrheagenic Escherichia coli strainsArvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
FEMS Microbiol Lett 227:39-45. 2003..minimal growth media...
Functional heterogeneity of RpoS in stress tolerance of enterohemorrhagic Escherichia coli strainsArvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
Appl Environ Microbiol 72:4978-86. 2006..Such adaptation processes may have significant impact on a pathogen's survival in food processing environments, as well in the host's stomach and intestine...
Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in miceArvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 002, BARC W, Beltsville, MD 20705 235, USA
Microbiology 155:229-37. 2009..0) and had lower ATP levels compared to the wild-type strain. These data indicate that OPGs of S. Typhimurium contribute towards mouse virulence as well as growth and motility under low osmolarity growth conditions...
Motility revertants of opgGH mutants of Salmonella enterica serovar Typhimurium remain defective in mice virulencePorteen Kannan
Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
Curr Microbiol 59:641-5. 2009..The data suggest that Salmonella may have distinctive regulatory elements in addition to Rcs phosphorelay genes to rescue motility of opg mutants and affecting also mouse virulence...
Characterization of Shiga toxin-producing Escherichia coli strains isolated from swine fecesPina M Fratamico
Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
Foodborne Pathog Dis 5:827-38. 2008..This study demonstrates that swine STEC comprise a heterogeneous group of organisms, and the possession of different combinations of E. coli virulence genes indicate that some swine STEC can potentially cause human illness...
Role of anionic charges of periplasmic glucans of Shigella flexneri in overcoming detergent stressArvind A Bhagwat
Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland 20705 2350, USA
Foodborne Pathog Dis 9:632-7. 2012..The data indicate that anionic charges on the OPGs contribute towards overcoming the stress caused by anionic detergents such as sodium dodecyl sulfate and sodium deoxycholate...
Characterization of enterohemorrhagic Escherichia coli strains based on acid resistance phenotypesArvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
Infect Immun 73:4993-5003. 2005..coli. Such mutations most likely alter the infectivity of individual isolates and may play a significant role in determining the infective dose of enterohemorrhagic E. coli...
Bacteriophage-based rapid and sensitive detection of Escherichia coli O157:H7 isolates from ground beefPorteen Kannan
Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705 2350, USA
Foodborne Pathog Dis 7:1551-8. 2010..The method is high-throughput compatible, is rapid, and can provide live culture the following day by streaking an aliquot before phage lysis on conventional selective agar media...
Role of RNA polymerase sigma-factor (RpoS) in induction of glutamate-dependent acid-resistance of Escherichia albertii under anaerobic conditionsJason Ling
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, USA
FEMS Microbiol Lett 283:75-82. 2008..The data indicate that certain E. albertii strains may have an alternate RpoS-dependent pathway for acid-resistance under anaerobic growth conditions...
Comparative analysis of transcriptional regulatory elements of glutamate-dependent acid-resistance systems of Shigella flexneri and Escherichia coli O157:H7Arvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
FEMS Microbiol Lett 234:139-47. 2004..flexneri is anticipated, which also may explain the observed differences in the GDAR of two enteric pathogens...
Characterization of Salmonella isolates from retail foods based on serotyping, pulse field gel electrophoresis, antibiotic resistance and other phenotypic propertiesXiaodong Xia
Produce Quality and Safety Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
Int J Food Microbiol 129:93-8. 2009..This study indicated that different serotypes of Salmonella were present in a variety of retail foods and exhibited diverse phenotypic characteristics...
Role of anionic charges of osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium SL1344 in mice virulenceArvind A Bhagwat
Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 173, BARC E, Beltsville, MD 20705 2350, USA
Arch Microbiol 194:541-8. 2012..Anionic substituents of OPGs from Salmonella although appear to be needed to overcome resistance of deoxycholate in hypoosmotic growth media, no evidence was found for their role in mice virulence...
Application of a molecular beacon-real-time PCR technology to detect Salmonella species contaminating fruits and vegetablesSamantha Hu Liming
Produce Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Building 002, Room 117, BARC-W, Beltsville, MD 20705-2350, USA
Int J Food Microbiol 95:177-87. 2004..This is the first report of the application of MB probe being used for real-time detection of Salmonella species in whole and fresh-cut fruits and vegetables...
Methods and tools for comparative genomics of foodborne pathogensArvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705 2350, USA
Foodborne Pathog Dis 5:487-97. 2008..These genome sequence analysis tools will strengthen foodborne pathogen surveillance and subsequent risk assessment to enhance the safety of the food supply...
Occurrence of inhibitory compounds in spent growth media that interfere with acid-tolerance mechanisms of enteric pathogensYingru Chen
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
Int J Food Microbiol 91:175-83. 2004..The low molecular weight (<300 Da) and heat resistant nature (121 degrees C, 15 min, at 15 psi) of the compounds make them excellent candidates to explore their suitability as food additives that would increase microbial food safety...
Detection of Salmonella species in foodstuffsArvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, USDA, Beltsville, MD, USA
Methods Mol Biol 429:33-43. 2008..The procedure uses the buffered peptone water for the enrichment of Salmonella spp. and successfully detects the pathogen at low level of contamination (2-4 cells/25 g) in <24 h...
Arginine-dependent acid-resistance pathway in Shigella boydiiKelvin Goh
Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 002, Room 117, BARC W, Beltsville, MD 20705 2350, USA
Arch Microbiol 193:179-85. 2011..5. ADAR pathway also appears to be present in all four Shigella sp. Shigella ADAR pathway was induced when cells were grown under partial oxygen pressure while its expression in E. coli required mere fermentative growth on glucose...
Sensitivity of Escherichia albertii, a potential food-borne pathogen, to food preservation treatmentsManan Sharma
Food Technology and Safety Laboratory, USDA ARS, ANRI, BARC East, Beltsville, MD 20705, USA
Appl Environ Microbiol 73:4351-3. 2007..Its tolerances to heat (56 degrees C), acid (pH 3.0), and pressure (500 MPa [5 min]) were evaluated and found to be significantly less than those of wild-type E. coli O157:H7...
Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCRArvind A Bhagwat
Produce Quality and Safety Laboratory, Building 002, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
Int J Food Microbiol 84:217-24. 2003..coli O157:H7 and S. typhimurium down to the predicted level of 1 to 10 cells/ml and L. monocytogenes at 1000 cells/ml. The ability to monitor several pathogens simultaneously will save time and increase our ability to assure food safety...
Rapid real-time PCR assay for detecting Salmonella in raw and ready-to-eat meatsJitu R Patel
Food Safety Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705 2350, USA
Acta Vet Hung 56:451-8. 2008....
Osmoregulated periplasmic glucans are needed for competitive growth and biofilm formation by Salmonella enterica serovar Typhimurium in leafy-green vegetable wash waters and colonization in miceLiu Liu
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
FEMS Microbiol Lett 292:13-20. 2009..This is the first report demonstrating the role of OPGs of Salmonella in competitive colonization of biofilms, planktonic cultures and mouse organs...
Computational methods and evaluation of RNA stabilization reagents for genome-wide expression studiesArvind A Bhagwat
Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 2350, USA
J Microbiol Methods 55:399-409. 2003..Scripts were also written to examine the occurrence of artifacts, based on GC content, length of the individual open reading frame (ORF), its distribution on plus and minus DNA strands, and the distance from the replication origin...
Inoculation onto solid surfaces protects Salmonella spp. during acid challenge: a model study using polyethersulfone membranesPurushottam V Gawande
Produce Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705-2350, USA
Appl Environ Microbiol 68:86-92. 2002..The surface-associated acid protection, which was observed in several Salmonella strains, required de novo protein synthesis and was independent of stationary-phase sigma transcription factor...
Characterization of ndvD, the third gene involved in the synthesis of cyclic beta-(1 --> 3),(1 --> 6)-D-glucans in Bradyrhizobium japonicumRongji Chen
Soybean Genomics and Improvement Laboratory, USDA-Agricultural Research Service, B-002, BARC-W, Beltsville, Maryland 20705, USA
Can J Microbiol 48:1008-16. 2002..Therefore, ndvD does not appear to be a structural gene for beta-glucan synthesis. Our hypothesis for the mechanism of beta-(1 --> 3),(1 --> 6)-D-glucan synthesis is presented...
A motility revertant of the ndvB mutant of Bradyrhizobium japonicumRongji Chen
Soybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Building 002, BARC-West, Beltsville, MD 20705, USA
Curr Microbiol 47:431-3. 2003..These results support our hypothesis that cyclic beta-glucans have an important role in symbiosis--the suppression of a plant defense response--in addition to their role in periplasmic osmoprotection...
Characterization of Listeria monocytogenes isolated from retail foodsYifan Zhang
Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA
Int J Food Microbiol 113:47-53. 2007..In addition, the presence of potential serotype 4b in all food categories is of public health concern, as serotype 4b has been the serotype most frequently associated with human listeriosis...
Isolation and characterization of Listeria monocytogenes isolates from ready-to-eat foods in FloridaYuelian Shen
Florida State Department of Agriculture and Consumer Services, Tallahassee, Florida, USA
Appl Environ Microbiol 72:5073-6. 2006..Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods...
