Arvind A Bhagwat

Summary

Affiliation: Agricultural Research Service
Country: USA

Publications

  1. ncbi request reprint Regulation of the glutamate-dependent acid-resistance system of diarrheagenic Escherichia coli strains
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    FEMS Microbiol Lett 227:39-45. 2003
  2. pmc Functional heterogeneity of RpoS in stress tolerance of enterohemorrhagic Escherichia coli strains
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    Appl Environ Microbiol 72:4978-86. 2006
  3. doi request reprint Motility revertants of opgGH mutants of Salmonella enterica serovar Typhimurium remain defective in mice virulence
    Porteen Kannan
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    Curr Microbiol 59:641-5. 2009
  4. doi request reprint Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 002, BARC W, Beltsville, MD 20705 235, USA
    Microbiology 155:229-37. 2009
  5. ncbi request reprint Characterization of Shiga toxin-producing Escherichia coli strains isolated from swine feces
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    Foodborne Pathog Dis 5:827-38. 2008
  6. doi request reprint Role of anionic charges of periplasmic glucans of Shigella flexneri in overcoming detergent stress
    Arvind A Bhagwat
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland 20705 2350, USA
    Foodborne Pathog Dis 9:632-7. 2012
  7. pmc Characterization of enterohemorrhagic Escherichia coli strains based on acid resistance phenotypes
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    Infect Immun 73:4993-5003. 2005
  8. doi request reprint Characterization of Salmonella isolates from retail foods based on serotyping, pulse field gel electrophoresis, antibiotic resistance and other phenotypic properties
    Xiaodong Xia
    Produce Quality and Safety Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    Int J Food Microbiol 129:93-8. 2009
  9. doi request reprint Bacteriophage-based rapid and sensitive detection of Escherichia coli O157:H7 isolates from ground beef
    Porteen Kannan
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705 2350, USA
    Foodborne Pathog Dis 7:1551-8. 2010
  10. doi request reprint Role of RNA polymerase sigma-factor (RpoS) in induction of glutamate-dependent acid-resistance of Escherichia albertii under anaerobic conditions
    Jason Ling
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, USA
    FEMS Microbiol Lett 283:75-82. 2008

Collaborators

  • Jianghong Meng
  • Manan Sharma
  • Pina M Fratamico
  • Kalmia E Kniel
  • Jitu R Patel
  • Mahendra H Kothary
  • Yan Liu
  • Allen Smith
  • Porteen Kannan
  • Rongji Chen
  • Yifan Zhang
  • Samantha Hu Liming
  • Kelvin Goh
  • Xiaodong Xia
  • Liu Liu
  • Jason Ling
  • Jennifer Cripe
  • Grace Hall
  • Yuelian Shen
  • Yingru Chen
  • Donald L Keister
  • Purushottam V Gawande
  • Brian Beck
  • Marian L McKee
  • Darren Chua
  • Claire Cleaver
  • Lucy Reiman
  • Pradip Patel
  • Ho Yao Yong
  • Jeffrey Karns
  • Mahesh Dharne
  • Won Jun
  • James McEvoy
  • Shawn Tan
  • Shaohua Zhao
  • Emily Yeh
  • William Conway
  • Robert Yaklich

Detail Information

Publications28

  1. ncbi request reprint Regulation of the glutamate-dependent acid-resistance system of diarrheagenic Escherichia coli strains
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    FEMS Microbiol Lett 227:39-45. 2003
    ..minimal growth media...
  2. pmc Functional heterogeneity of RpoS in stress tolerance of enterohemorrhagic Escherichia coli strains
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    Appl Environ Microbiol 72:4978-86. 2006
    ..Such adaptation processes may have significant impact on a pathogen's survival in food processing environments, as well in the host's stomach and intestine...
  3. doi request reprint Motility revertants of opgGH mutants of Salmonella enterica serovar Typhimurium remain defective in mice virulence
    Porteen Kannan
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    Curr Microbiol 59:641-5. 2009
    ..The data suggest that Salmonella may have distinctive regulatory elements in addition to Rcs phosphorelay genes to rescue motility of opg mutants and affecting also mouse virulence...
  4. doi request reprint Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 002, BARC W, Beltsville, MD 20705 235, USA
    Microbiology 155:229-37. 2009
    ..0) and had lower ATP levels compared to the wild-type strain. These data indicate that OPGs of S. Typhimurium contribute towards mouse virulence as well as growth and motility under low osmolarity growth conditions...
  5. ncbi request reprint Characterization of Shiga toxin-producing Escherichia coli strains isolated from swine feces
    Pina M Fratamico
    Eastern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, Wyndmoor, Pennsylvania 19038, USA
    Foodborne Pathog Dis 5:827-38. 2008
    ..This study demonstrates that swine STEC comprise a heterogeneous group of organisms, and the possession of different combinations of E. coli virulence genes indicate that some swine STEC can potentially cause human illness...
  6. doi request reprint Role of anionic charges of periplasmic glucans of Shigella flexneri in overcoming detergent stress
    Arvind A Bhagwat
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland 20705 2350, USA
    Foodborne Pathog Dis 9:632-7. 2012
    ..The data indicate that anionic charges on the OPGs contribute towards overcoming the stress caused by anionic detergents such as sodium dodecyl sulfate and sodium deoxycholate...
  7. pmc Characterization of enterohemorrhagic Escherichia coli strains based on acid resistance phenotypes
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    Infect Immun 73:4993-5003. 2005
    ..coli. Such mutations most likely alter the infectivity of individual isolates and may play a significant role in determining the infective dose of enterohemorrhagic E. coli...
  8. doi request reprint Characterization of Salmonella isolates from retail foods based on serotyping, pulse field gel electrophoresis, antibiotic resistance and other phenotypic properties
    Xiaodong Xia
    Produce Quality and Safety Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    Int J Food Microbiol 129:93-8. 2009
    ..This study indicated that different serotypes of Salmonella were present in a variety of retail foods and exhibited diverse phenotypic characteristics...
  9. doi request reprint Bacteriophage-based rapid and sensitive detection of Escherichia coli O157:H7 isolates from ground beef
    Porteen Kannan
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705 2350, USA
    Foodborne Pathog Dis 7:1551-8. 2010
    ..The method is high-throughput compatible, is rapid, and can provide live culture the following day by streaking an aliquot before phage lysis on conventional selective agar media...
  10. doi request reprint Role of RNA polymerase sigma-factor (RpoS) in induction of glutamate-dependent acid-resistance of Escherichia albertii under anaerobic conditions
    Jason Ling
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD, USA
    FEMS Microbiol Lett 283:75-82. 2008
    ..The data indicate that certain E. albertii strains may have an alternate RpoS-dependent pathway for acid-resistance under anaerobic growth conditions...
  11. ncbi request reprint Comparative analysis of transcriptional regulatory elements of glutamate-dependent acid-resistance systems of Shigella flexneri and Escherichia coli O157:H7
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Bldg 002, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    FEMS Microbiol Lett 234:139-47. 2004
    ..flexneri is anticipated, which also may explain the observed differences in the GDAR of two enteric pathogens...
  12. doi request reprint Role of anionic charges of osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium SL1344 in mice virulence
    Arvind A Bhagwat
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 173, BARC E, Beltsville, MD 20705 2350, USA
    Arch Microbiol 194:541-8. 2012
    ..Anionic substituents of OPGs from Salmonella although appear to be needed to overcome resistance of deoxycholate in hypoosmotic growth media, no evidence was found for their role in mice virulence...
  13. ncbi request reprint Application of a molecular beacon-real-time PCR technology to detect Salmonella species contaminating fruits and vegetables
    Samantha Hu Liming
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Building 002, Room 117, BARC W, Beltsville, MD 20705 2350, USA
    Int J Food Microbiol 95:177-87. 2004
    ..This is the first report of the application of MB probe being used for real-time detection of Salmonella species in whole and fresh-cut fruits and vegetables...
  14. pmc Methods and tools for comparative genomics of foodborne pathogens
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705 2350, USA
    Foodborne Pathog Dis 5:487-97. 2008
    ..These genome sequence analysis tools will strengthen foodborne pathogen surveillance and subsequent risk assessment to enhance the safety of the food supply...
  15. ncbi request reprint Detection of Salmonella species in foodstuffs
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, USDA, Beltsville, MD, USA
    Methods Mol Biol 429:33-43. 2008
    ..The procedure uses the buffered peptone water for the enrichment of Salmonella spp. and successfully detects the pathogen at low level of contamination (2-4 cells/25 g) in <24 h...
  16. ncbi request reprint Occurrence of inhibitory compounds in spent growth media that interfere with acid-tolerance mechanisms of enteric pathogens
    Yingru Chen
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    Int J Food Microbiol 91:175-83. 2004
    ..The low molecular weight (<300 Da) and heat resistant nature (121 degrees C, 15 min, at 15 psi) of the compounds make them excellent candidates to explore their suitability as food additives that would increase microbial food safety...
  17. pmc Sensitivity of Escherichia albertii, a potential food-borne pathogen, to food preservation treatments
    Manan Sharma
    Food Technology and Safety Laboratory, USDA ARS, ANRI, BARC East, Beltsville, MD 20705, USA
    Appl Environ Microbiol 73:4351-3. 2007
    ..Its tolerances to heat (56 degrees C), acid (pH 3.0), and pressure (500 MPa [5 min]) were evaluated and found to be significantly less than those of wild-type E. coli O157:H7...
  18. doi request reprint Arginine-dependent acid-resistance pathway in Shigella boydii
    Kelvin Goh
    Environmental Microbial and Food Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Bldg 002, Room 117, BARC W, Beltsville, MD 20705 2350, USA
    Arch Microbiol 193:179-85. 2011
    ..5. ADAR pathway also appears to be present in all four Shigella sp. Shigella ADAR pathway was induced when cells were grown under partial oxygen pressure while its expression in E. coli required mere fermentative growth on glucose...
  19. doi request reprint Osmoregulated periplasmic glucans are needed for competitive growth and biofilm formation by Salmonella enterica serovar Typhimurium in leafy-green vegetable wash waters and colonization in mice
    Liu Liu
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, MD 20705 2350, USA
    FEMS Microbiol Lett 292:13-20. 2009
    ..This is the first report demonstrating the role of OPGs of Salmonella in competitive colonization of biofilms, planktonic cultures and mouse organs...
  20. ncbi request reprint Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCR
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Building 002, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, MD 20705 2350, USA
    Int J Food Microbiol 84:217-24. 2003
    ..coli O157:H7 and S. typhimurium down to the predicted level of 1 to 10 cells/ml and L. monocytogenes at 1000 cells/ml. The ability to monitor several pathogens simultaneously will save time and increase our ability to assure food safety...
  21. doi request reprint Rapid real-time PCR assay for detecting Salmonella in raw and ready-to-eat meats
    Jitu R Patel
    Food Safety Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705 2350, USA
    Acta Vet Hung 56:451-8. 2008
    ....
  22. doi request reprint Hypervirulent-host-associated Citrobacter rodentium cells have poor acid tolerance
    Allen Smith
    Diet Genomics and Immunology and Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, BARC E, Beltsville, MD 20705 2350, USA
    Curr Microbiol 66:522-6. 2013
    ..Thus, contrary to conventional anticipation, hypervirulent C. rodentium cells were found to be acid sensitive thereby revealing limitations of the role of mouse gastric acidity by itself in elucidating the hypervirulent phenotype...
  23. ncbi request reprint Computational methods and evaluation of RNA stabilization reagents for genome-wide expression studies
    Arvind A Bhagwat
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 2350, USA
    J Microbiol Methods 55:399-409. 2003
    ..Scripts were also written to examine the occurrence of artifacts, based on GC content, length of the individual open reading frame (ORF), its distribution on plus and minus DNA strands, and the distance from the replication origin...
  24. pmc Inoculation onto solid surfaces protects Salmonella spp. during acid challenge: a model study using polyethersulfone membranes
    Purushottam V Gawande
    Produce Quality and Safety Laboratory, Henry A Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA, Beltsville, Maryland 20705 2350, USA
    Appl Environ Microbiol 68:86-92. 2002
    ..The surface-associated acid protection, which was observed in several Salmonella strains, required de novo protein synthesis and was independent of stationary-phase sigma transcription factor...
  25. ncbi request reprint A motility revertant of the ndvB mutant of Bradyrhizobium japonicum
    Rongji Chen
    Soybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Building 002, BARC West, Beltsville, MD 20705, USA
    Curr Microbiol 47:431-3. 2003
    ..These results support our hypothesis that cyclic beta-glucans have an important role in symbiosis--the suppression of a plant defense response--in addition to their role in periplasmic osmoprotection...
  26. ncbi request reprint Characterization of ndvD, the third gene involved in the synthesis of cyclic beta-(1 --> 3),(1 --> 6)-D-glucans in Bradyrhizobium japonicum
    Rongji Chen
    Soybean Genomics and Improvement Laboratory, USDA Agricultural Research Service, B 002, BARC W, Beltsville, Maryland 20705, USA
    Can J Microbiol 48:1008-16. 2002
    ..Therefore, ndvD does not appear to be a structural gene for beta-glucan synthesis. Our hypothesis for the mechanism of beta-(1 --> 3),(1 --> 6)-D-glucan synthesis is presented...
  27. ncbi request reprint Characterization of Listeria monocytogenes isolated from retail foods
    Yifan Zhang
    Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA
    Int J Food Microbiol 113:47-53. 2007
    ..In addition, the presence of potential serotype 4b in all food categories is of public health concern, as serotype 4b has been the serotype most frequently associated with human listeriosis...
  28. pmc Isolation and characterization of Listeria monocytogenes isolates from ready-to-eat foods in Florida
    Yuelian Shen
    Florida State Department of Agriculture and Consumer Services, Tallahassee, Florida, USA
    Appl Environ Microbiol 72:5073-6. 2006
    ..Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods...