Qiaoqiao Ruan

Summary

Affiliation: Abbott Laboratories
Country: USA

Publications

  1. pmc Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS)
    Qiaoqiao Ruan
    Department of Biophysics, University of Illinois in Urbana Champaign, Urbana, IL, USA
    Biophys J 87:1260-7. 2004
  2. pmc Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopy
    Qiaoqiao Ruan
    Laboratory for Fluorescence Dynamics, Department, University of Illinois in Urbana Champaign, 1110 W Green Street, Urbana, IL 61801, USA
    Biophys J 83:3177-87. 2002
  3. doi request reprint Microheterogeneous monoclonal antibody subspecies with differential hepatitis C virus core antigen binding properties identified by SEC-HPLC
    A Scott Muerhoff
    Infectious Diseases Research and Development, Abbott Diagnostics, Abbott Laboratories, Abbott Park, IL 60064 6015, USA
    J Immunol Methods 345:60-9. 2009
  4. ncbi request reprint Interactions of two monoclonal antibodies with BNP: high resolution epitope mapping using fluorescence correlation spectroscopy
    Sergey Y Tetin
    Biotechnology, Core Research and Development, Diagnostics Division, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064, USA
    Biochemistry 45:14155-65. 2006
  5. pmc Chromatin dynamics in interphase cells revealed by tracking in a two-photon excitation microscope
    Valeria Levi
    Laboratory for Fluorescence Dynamics, and Department of Cell and Structural Biology, Chemical and Life Science Laboratory, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3080, USA
    Biophys J 89:4275-85. 2005
  6. ncbi request reprint Application of fluorescence correlation spectroscopy to hapten-antibody binding
    Theodore L Hazlett
    Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana Champaign, Urbana, IL, USA
    Methods Mol Biol 305:415-38. 2005
  7. pmc 3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells
    Valeria Levi
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3080, USA
    Biophys J 88:2919-28. 2005
  8. pmc Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy
    Yan Chen
    Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 82:133-44. 2002
  9. ncbi request reprint Applications of dual-color fluorescence cross-correlation spectroscopy in antibody binding studies
    Qiaoqiao Ruan
    Department of Biotechnology, Core Research and Development, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064, USA
    Anal Biochem 374:182-95. 2008
  10. ncbi request reprint Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods
    Enrico Gratton
    University of Illinois at Urbana Champaign, Laboratory for Fluorescence Dynamics, 1110 West Green Street Urbana, Illinois 61801, USA
    J Biomed Opt 8:381-90. 2003

Detail Information

Publications10

  1. pmc Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS)
    Qiaoqiao Ruan
    Department of Biophysics, University of Illinois in Urbana Champaign, Urbana, IL, USA
    Biophys J 87:1260-7. 2004
    ..Scanning FCS provided a simple, quantitative, yet highly sensitive method to study protein-membrane interactions...
  2. pmc Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopy
    Qiaoqiao Ruan
    Laboratory for Fluorescence Dynamics, Department, University of Illinois in Urbana Champaign, 1110 W Green Street, Urbana, IL 61801, USA
    Biophys J 83:3177-87. 2002
    ..This technology offers advantages in studying protein interactions and function in the cell...
  3. doi request reprint Microheterogeneous monoclonal antibody subspecies with differential hepatitis C virus core antigen binding properties identified by SEC-HPLC
    A Scott Muerhoff
    Infectious Diseases Research and Development, Abbott Diagnostics, Abbott Laboratories, Abbott Park, IL 60064 6015, USA
    J Immunol Methods 345:60-9. 2009
    ..The choice of size exclusion column matrix and buffer composition was critical to the identification of these monoclonal IgG subspecies...
  4. ncbi request reprint Interactions of two monoclonal antibodies with BNP: high resolution epitope mapping using fluorescence correlation spectroscopy
    Sergey Y Tetin
    Biotechnology, Core Research and Development, Diagnostics Division, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064, USA
    Biochemistry 45:14155-65. 2006
    ..Monoclonal antibodies 106.3 and BC203 demonstrate high affinities to BNP and bind two distant epitopes forming robust antibody sandwiches. Both antibodies are used in Abbott diagnostic assays on AxSYM, IMx, and Architect platforms...
  5. pmc Chromatin dynamics in interphase cells revealed by tracking in a two-photon excitation microscope
    Valeria Levi
    Laboratory for Fluorescence Dynamics, and Department of Cell and Structural Biology, Chemical and Life Science Laboratory, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3080, USA
    Biophys J 89:4275-85. 2005
    ..Additionally, the jumps were sensitive to the temperature and absent after ATP depletion. These experimental results point to an energy-dependent mechanism driving fast motion of chromatin in interphase cells...
  6. ncbi request reprint Application of fluorescence correlation spectroscopy to hapten-antibody binding
    Theodore L Hazlett
    Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana Champaign, Urbana, IL, USA
    Methods Mol Biol 305:415-38. 2005
    ..Clearly, concentration assays based on IgG rely on accurate knowledge of the hapten-IgG binding strengths. The protocols for measuring and determining the dissociation constants for both IgG-hapten pairs are outlined and discussed...
  7. pmc 3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells
    Valeria Levi
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3080, USA
    Biophys J 88:2919-28. 2005
    ..7 cP. The feasibility of this method for live cell measurements is demonstrated studying the phagocytosis of protein-coated fluorospheres by fibroblasts...
  8. pmc Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy
    Yan Chen
    Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 82:133-44. 2002
    ..We found the molecular brightness of EGFP to be a very robust parameter, and anticipate the use of PCH analysis for the study of oligomerization processes in vivo...
  9. ncbi request reprint Applications of dual-color fluorescence cross-correlation spectroscopy in antibody binding studies
    Qiaoqiao Ruan
    Department of Biotechnology, Core Research and Development, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064, USA
    Anal Biochem 374:182-95. 2008
    ..For systems containing proteins of a similar size that interact without substantial changes in the fluorescence intensity, DC-FCCS serves as a preferred means of measuring solution phase binding constants...
  10. ncbi request reprint Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods
    Enrico Gratton
    University of Illinois at Urbana Champaign, Laboratory for Fluorescence Dynamics, 1110 West Green Street Urbana, Illinois 61801, USA
    J Biomed Opt 8:381-90. 2003
    ..While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples...