Affiliation: Abbott Laboratories
- Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS)Qiaoqiao Ruan
Department of Biophysics, University of Illinois in Urbana Champaign, Urbana, IL, USA
Biophys J 87:1260-7. 2004..Scanning FCS provided a simple, quantitative, yet highly sensitive method to study protein-membrane interactions...
- Cellular characterization of adenylate kinase and its isoform: two-photon excitation fluorescence imaging and fluorescence correlation spectroscopyQiaoqiao Ruan
Laboratory for Fluorescence Dynamics, Department, University of Illinois in Urbana Champaign, 1110 W Green Street, Urbana, IL 61801, USA
Biophys J 83:3177-87. 2002..This technology offers advantages in studying protein interactions and function in the cell...
- Microheterogeneous monoclonal antibody subspecies with differential hepatitis C virus core antigen binding properties identified by SEC-HPLCA Scott Muerhoff
Infectious Diseases Research and Development, Abbott Diagnostics, Abbott Laboratories, Abbott Park, IL 60064 6015, USA
J Immunol Methods 345:60-9. 2009..The choice of size exclusion column matrix and buffer composition was critical to the identification of these monoclonal IgG subspecies...
- Interactions of two monoclonal antibodies with BNP: high resolution epitope mapping using fluorescence correlation spectroscopySergey Y Tetin
Biotechnology, Core Research and Development, Diagnostics Division, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064, USA
Biochemistry 45:14155-65. 2006..Monoclonal antibodies 106.3 and BC203 demonstrate high affinities to BNP and bind two distant epitopes forming robust antibody sandwiches. Both antibodies are used in Abbott diagnostic assays on AxSYM, IMx, and Architect platforms...
- Chromatin dynamics in interphase cells revealed by tracking in a two-photon excitation microscopeValeria Levi
Laboratory for Fluorescence Dynamics, and Department of Cell and Structural Biology, Chemical and Life Science Laboratory, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3080, USA
Biophys J 89:4275-85. 2005..Additionally, the jumps were sensitive to the temperature and absent after ATP depletion. These experimental results point to an energy-dependent mechanism driving fast motion of chromatin in interphase cells...
- Application of fluorescence correlation spectroscopy to hapten-antibody bindingTheodore L Hazlett
Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana Champaign, Urbana, IL, USA
Methods Mol Biol 305:415-38. 2005..Clearly, concentration assays based on IgG rely on accurate knowledge of the hapten-IgG binding strengths. The protocols for measuring and determining the dissociation constants for both IgG-hapten pairs are outlined and discussed...
- 3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cellsValeria Levi
Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3080, USA
Biophys J 88:2919-28. 2005..7 cP. The feasibility of this method for live cell measurements is demonstrated studying the phagocytosis of protein-coated fluorospheres by fibroblasts...
- Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopyYan Chen
Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
Biophys J 82:133-44. 2002..We found the molecular brightness of EGFP to be a very robust parameter, and anticipate the use of PCH analysis for the study of oligomerization processes in vivo...
- Applications of dual-color fluorescence cross-correlation spectroscopy in antibody binding studiesQiaoqiao Ruan
Department of Biotechnology, Core Research and Development, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064, USA
Anal Biochem 374:182-95. 2008..For systems containing proteins of a similar size that interact without substantial changes in the fluorescence intensity, DC-FCCS serves as a preferred means of measuring solution phase binding constants...
- Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methodsEnrico Gratton
University of Illinois at Urbana Champaign, Laboratory for Fluorescence Dynamics, 1110 West Green Street Urbana, Illinois 61801, USA
J Biomed Opt 8:381-90. 2003..While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples...