R Radi

Summary

Affiliation: Facultad de Medicina
Country: Uruguay

Publications

  1. ncbi request reprint Unraveling peroxynitrite formation in biological systems
    R Radi
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 30:463-88. 2001
  2. pmc Nitric oxide, oxidants, and protein tyrosine nitration
    Rafael Radi
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, 11800 Montevideo, Uruguay
    Proc Natl Acad Sci U S A 101:4003-8. 2004
  3. ncbi request reprint Peroxynitrite reactions and formation in mitochondria
    Rafael Radi
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 33:1451-64. 2002
  4. ncbi request reprint Nitric oxide and peroxynitrite interactions with mitochondria
    Rafael Radi
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biol Chem 383:401-9. 2002
  5. ncbi request reprint Immuno-spin trapping: a breakthrough for the sensitive detection of protein-derived radicals, a commentary on "Protein radical formation on thyroid peroxidase during turnover"
    Rafael Radi
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo 11800, Uruguay
    Free Radic Biol Med 41:416-7. 2006
  6. ncbi request reprint Peroxynitrite decay in the presence of hydrogen peroxide, mannitol and ethanol: a reappraisal
    B Alvarez
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Res 34:467-75. 2001
  7. ncbi request reprint How I became a Biochemist
    Rafael Radi
    Department of Biochemistry and Center for Free Radical and Biomedical Research Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    IUBMB Life 58:559-61. 2006
  8. ncbi request reprint Sulfenic acid formation in human serum albumin by hydrogen peroxide and peroxynitrite
    Sebastián Carballal
    Laboratorio De Enzimologia, Facultad de Ciencias and Departamento de Bioquímica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochemistry 42:9906-14. 2003
  9. doi request reprint Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants, and conformational dynamics
    Martín Hugo
    Departamento de Bioqumica, Facultad de Medicina, Center for Free Radical and Biomedical Research, Universidad de la Republica, Montevideo, Uruguay
    Biochemistry 48:9416-26. 2009
  10. ncbi request reprint Nitric oxide diffusion in membranes determined by fluorescence quenching
    A Denicola
    Department of Biochemistry, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Arch Biochem Biophys 328:208-12. 1996

Collaborators

Detail Information

Publications89

  1. ncbi request reprint Unraveling peroxynitrite formation in biological systems
    R Radi
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 30:463-88. 2001
    ....
  2. pmc Nitric oxide, oxidants, and protein tyrosine nitration
    Rafael Radi
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, 11800 Montevideo, Uruguay
    Proc Natl Acad Sci U S A 101:4003-8. 2004
    ....
  3. ncbi request reprint Peroxynitrite reactions and formation in mitochondria
    Rafael Radi
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 33:1451-64. 2002
    ....
  4. ncbi request reprint Nitric oxide and peroxynitrite interactions with mitochondria
    Rafael Radi
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biol Chem 383:401-9. 2002
    ..In this review we will provide an overview of the biochemical mechanisms by which *NO and ONOO- regulate or alter mitochondrial functions...
  5. ncbi request reprint Immuno-spin trapping: a breakthrough for the sensitive detection of protein-derived radicals, a commentary on "Protein radical formation on thyroid peroxidase during turnover"
    Rafael Radi
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo 11800, Uruguay
    Free Radic Biol Med 41:416-7. 2006
  6. ncbi request reprint Peroxynitrite decay in the presence of hydrogen peroxide, mannitol and ethanol: a reappraisal
    B Alvarez
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Res 34:467-75. 2001
    ..In addition, by simulations using known rate constants and absorption coefficients, we show that the slowing effect can be quantitatively accounted for by the formation of peroxynitrate...
  7. ncbi request reprint How I became a Biochemist
    Rafael Radi
    Department of Biochemistry and Center for Free Radical and Biomedical Research Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    IUBMB Life 58:559-61. 2006
  8. ncbi request reprint Sulfenic acid formation in human serum albumin by hydrogen peroxide and peroxynitrite
    Sebastián Carballal
    Laboratorio De Enzimologia, Facultad de Ciencias and Departamento de Bioquímica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochemistry 42:9906-14. 2003
    ....
  9. doi request reprint Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants, and conformational dynamics
    Martín Hugo
    Departamento de Bioqumica, Facultad de Medicina, Center for Free Radical and Biomedical Research, Universidad de la Republica, Montevideo, Uruguay
    Biochemistry 48:9416-26. 2009
    ..Taken together, our results indicate that MtAhpE is a relevant component in the antioxidant repertoire of M. tuberculosis probably involved in peroxide and specially peroxynitrite detoxification...
  10. ncbi request reprint Nitric oxide diffusion in membranes determined by fluorescence quenching
    A Denicola
    Department of Biochemistry, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Arch Biochem Biophys 328:208-12. 1996
    ....
  11. ncbi request reprint Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment
    L Turell
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de Republica, Montevideo, Uruguay
    Braz J Med Biol Res 42:305-11. 2009
    ..Oxidized forms of albumin are increased in different pathophysiological conditions and sulfenic acid lies in a mechanistic junction, relating oxidizing species to final thiol oxidation products...
  12. ncbi request reprint Peroxynitrite-derived carbonate and nitrogen dioxide radicals readily react with lipoic and dihydrolipoic acid
    Madia Trujillo
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    Free Radic Biol Med 39:279-88. 2005
    ..The mechanisms of reaction defined herein allow to rationalize the biochemistry of peroxynitrite based on direct and free radical-mediated processes and contribute to the understanding of the antioxidant actions of LA and DHLA...
  13. ncbi request reprint Inactivation of human Cu,Zn superoxide dismutase by peroxynitrite and formation of histidinyl radical
    Beatriz Alvarez
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, 11400 Montevideo, Uruguay
    Free Radic Biol Med 37:813-22. 2004
    ....
  14. ncbi request reprint Tyrosine nitration by superoxide and nitric oxide fluxes in biological systems: modeling the impact of superoxide dismutase and nitric oxide diffusion
    Celia Quijano
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 39:728-41. 2005
    ..Thus, the model presented herein serves to reconcile the in vitro and in vivo evidence on the role of peroxynitrite in promoting tyrosine nitration...
  15. ncbi request reprint The trypanothione-thiol system in Trypanosoma cruzi as a key antioxidant mechanism against peroxynitrite-mediated cytotoxicity
    Leonor Thomson
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Igvá 4225, 11400 Montevideo, Uruguay
    Arch Biochem Biophys 412:55-64. 2003
    ..cruzi against peroxynitrite, which may facilitate the survival of trypanosomes within the oxidative environment of activated macrophages...
  16. ncbi request reprint Nitric oxide-derived oxidants with a focus on peroxynitrite: molecular targets, cellular responses and therapeutic implications
    P Calcerrada
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, 11800, Montevideo, Uruguay
    Curr Pharm Des 17:3905-32. 2011
    ..In addition, a selection of available pharmacological tools will be discussed as an effort to rationalize antioxidant and/or redox-based therapeutic interventions in disease models...
  17. ncbi request reprint Nitric oxide reaction with lipid peroxyl radicals spares alpha-tocopherol during lipid peroxidation. Greater oxidant protection from the pair nitric oxide/alpha-tocopherol than alpha-tocopherol/ascorbate
    H Rubbo
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    J Biol Chem 275:10812-8. 2000
    ..NO, its capacity to diffuse and concentrate in lipophilic milieu, and a potent reactivity toward lipid radical species reveal how (.)NO can play a critical role in regulating membrane and lipoprotein lipid oxidation reactions...
  18. ncbi request reprint Trypanosoma brucei and Trypanosoma cruzi tryparedoxin peroxidases catalytically detoxify peroxynitrite via oxidation of fast reacting thiols
    Madia Trujillo
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Avda Gral Flores 2125, 11800 Montevideo, Uruguay
    J Biol Chem 279:34175-82. 2004
    ....
  19. ncbi request reprint Enhanced mitochondrial superoxide in hyperglycemic endothelial cells: direct measurements and formation of hydrogen peroxide and peroxynitrite
    Celia Quijano
    Departamento de Bioquimica, Facultad de Medicina, Avenida General Flores 2125, 11800 Montevideo, Uruguay
    Am J Physiol Heart Circ Physiol 293:H3404-14. 2007
    ..Mitochondrial O(2)(.-) formation in hyperglycemic conditions was associated with increased glucose metabolization in the Krebs cycle and hyperpolarization of the mitochondrial membrane...
  20. ncbi request reprint Cytochrome c-catalyzed oxidation of organic molecules by hydrogen peroxide
    R Radi
    Department of Biochemistry, Faculty of Medicine, University of the Republic, Montevideo, Uruguay
    Arch Biochem Biophys 288:112-7. 1991
    ....
  21. doi request reprint Chemical biology of peroxynitrite: kinetics, diffusion, and radicals
    Gerardo Ferrer-Sueta
    Laboratorio de Fisicoquímica Biológica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11800, Uruguay
    ACS Chem Biol 4:161-77. 2009
    ..Finally, we have assessed the most successful attempts to intercept peroxynitrite by pharmacological intervention in their potential to increment the existing biological defenses that routinely deal with this cytotoxin...
  22. ncbi request reprint Reaction of human hemoglobin with peroxynitrite. Isomerization to nitrate and secondary formation of protein radicals
    Natalia Romero
    Departamento de Bioquimica, Facultad de Medicina, 11800 Montevideo, Uruguay
    J Biol Chem 278:44049-57. 2003
    ..The results obtained support a role of oxyhemoglobin as a relevant intravascular sink of peroxynitrite...
  23. ncbi request reprint Peroxynitrite reaction with the reduced and the oxidized forms of lipoic acid: new insights into the reaction of peroxynitrite with thiols
    Madia Trujillo
    Departamento de Bioquimica, Universidad de la Republica, 11800 Montevideo, Uruguay
    Arch Biochem Biophys 397:91-8. 2002
    ..4. The pK(SH) for DHLA is 10.7, in agreement with its modest reactivity with peroxynitrite...
  24. ncbi request reprint Peroxynitrite reactivity with amino acids and proteins
    B Alvarez
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    Amino Acids 25:295-311. 2003
    ..The preferential protein targets of peroxynitrite and the role of proteins in peroxynitrite detoxifying pathways are discussed...
  25. ncbi request reprint Glyceraldehyde-3-phosphate dehydrogenase inactivation by peroxynitrite
    J M Souza
    Facultad de Medicina, Universidad de la Republica, Avenida Gral Flores 2125, Montevideo, 11800, Uruguay
    Arch Biochem Biophys 360:187-94. 1998
    ....
  26. ncbi request reprint Formation of lipid-protein adducts in low-density lipoprotein by fluxes of peroxynitrite and its inhibition by nitric oxide
    A Trostchansky
    , Facultad de Medicina, , Montevideo, Uruguay
    Arch Biochem Biophys 395:225-32. 2001
    ..The balance between rates of PN and *NO production in the vascular wall will critically determine the final extent of LDL oxidative modifications leading or not to scavenger receptor-mediated LDL uptake and foam cell formation...
  27. pmc Enzymes of the antioxidant network as novel determiners of Trypanosoma cruzi virulence
    L Piacenza
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Int J Parasitol 39:1455-64. 2009
    ..Our data support that enzymes of the parasite antioxidant armamentarium at the onset of infection represent new virulence factors involved in the establishment of disease...
  28. ncbi request reprint Interactions between nitric oxide and peroxynitrite during prostaglandin endoperoxide H synthase-1 catalysis: a free radical mechanism of inactivation
    Andrés Trostchansky
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    Free Radic Biol Med 42:1029-38. 2007
    ..Finally, our results reconcile the proposed alternative effects of ONOO(-) on PGHS-1 (activation versus inactivation)...
  29. ncbi request reprint Time course and site(s) of cytochrome c tyrosine nitration by peroxynitrite
    Carlos Batthyany
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochemistry 44:8038-46. 2005
    ..Moreover, identification of cytochrome c nitration sites in vivo may assist in unraveling the chemical nature of proximal reactive nitrogen species...
  30. ncbi request reprint Homolytic pathways drive peroxynitrite-dependent Trolox C oxidation
    Horacio Botti
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Chem Res Toxicol 17:1377-84. 2004
    ..Taken together, our results strongly support that H(+)- or CO(2)-catalyzed homolysis of peroxynitrite is required to cause TxOH, and hence, alpha-tocopherol oxidation...
  31. ncbi request reprint Reactions of manganese porphyrins and manganese-superoxide dismutase with peroxynitrite
    Gerardo Ferrer-Sueta
    Department of Physical Chemical Biology, Universidad de la Republica, 11800 Montevideo, Uruguay
    Methods Enzymol 349:23-37. 2002
  32. ncbi request reprint Red blood cells in the metabolism of nitric oxide-derived peroxynitrite
    Natalia Romero
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    IUBMB Life 58:572-80. 2006
    ..The intravascular formation, reactions and detoxification of peroxynitrite are revealed as important factors controlling vascular dysfunction and degeneration in a variety of pathophysiologically-relevant conditions...
  33. ncbi request reprint Reduction of manganese porphyrins by flavoenzymes and submitochondrial particles: a catalytic cycle for the reduction of peroxynitrite
    Gerardo Ferrer-Sueta
    Laboratorio de Fisicoquímica Biológica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11400, Uruguay
    Free Radic Biol Med 41:503-12. 2006
    ..The data presented here serve to rationalize the pharmacological action of MnPorphyrins as peroxynitrite reduction catalysts in vivo and opens avenues for the development of MnPorphyrins to protect mitochondria from oxidative damage...
  34. ncbi request reprint Sulfenic acid in human serum albumin
    S Carballal
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    Amino Acids 32:543-51. 2007
    ..Sulfenic acid in HSA could be involved in mixed disufide formation, supporting a role of HSA-Cys34 as an important redox regulator in extracellular compartments...
  35. ncbi request reprint Protein 3-nitrotyrosine formation during Trypanosoma cruzi infection in mice
    M Naviliat
    Departamento de Anatomia Patologica, Hospital de Clinicas, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, 11800 Montevideo, Uruguay
    Braz J Med Biol Res 38:1825-34. 2005
    ..NO, but also on its metabolic fate, including the generation of nitrating species that may constitute an important element in parasite resistance and collateral myocardial damage...
  36. ncbi request reprint Protein tyrosine nitration in hydrophilic and hydrophobic environments
    S Bartesaghi
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Amino Acids 32:501-15. 2007
    ....
  37. ncbi request reprint Nitric oxide-oxygen radicals interactions in atherosclerosis
    H Rubbo
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biol Res 33:167-75. 2000
    ....
  38. ncbi request reprint Kinetics of peroxiredoxins and their role in the decomposition of peroxynitrite
    Madia Trujillo
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    Subcell Biochem 44:83-113. 2007
    ..In higher organisms, their specific role is seen in the regulation of signalling cascades that are modulated by H2O2, lipid hydroperoxides or peroxynitrite...
  39. ncbi request reprint Reaction of the carbonate radical with the spin-trap 5,5-dimethyl-1-pyrroline-N-oxide in chemical and cellular systems: pulse radiolysis, electron paramagnetic resonance, and kinetic-competition studies
    María Noel Alvarez
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, 11800 Montevideo, Uruguay
    Free Radic Biol Med 43:1523-33. 2007
    ....
  40. pmc Mitochondrial dysfunction in SOD1G93A-bearing astrocytes promotes motor neuron degeneration: prevention by mitochondrial-targeted antioxidants
    Patricia Cassina
    Departamento de Histologia, and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    J Neurosci 28:4115-22. 2008
    ..Together, our results indicate that mitochondrial dysfunction in astrocytes critically influences motor neuron survival and support the potential pharmacological utility of mitochondrial-targeted antioxidants in ALS treatment...
  41. ncbi request reprint Pre-steady state kinetic characterization of human peroxiredoxin 5: taking advantage of Trp84 fluorescence increase upon oxidation
    Madia Trujillo
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, 11800 Montevideo, Uruguay
    Arch Biochem Biophys 467:95-106. 2007
    ....
  42. ncbi request reprint Tyrosine nitration, dimerization, and hydroxylation by peroxynitrite in membranes as studied by the hydrophobic probe N-t-BOC-l-tyrosine tert-butyl ester
    Silvina Bartesaghi
    Department of Biochemistry, Facultad de Medicina Universidad de la República, Montevideo, Uruguay
    Methods Enzymol 441:217-36. 2008
    ..The methods presented herein can be extended to explore the reaction mechanisms of tyrosine oxidation by other biologically relevant oxidants and in other hydrophobic biocompartments...
  43. pmc Lipid peroxyl radicals mediate tyrosine dimerization and nitration in membranes
    Silvina Bartesaghi
    Departamento de Histologia y Embriologia, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, 11800 Montevideo, Uruguay
    Chem Res Toxicol 23:821-35. 2010
    ....
  44. pmc Superoxide-mediated inactivation of nitric oxide and peroxynitrite formation by tobacco smoke in vascular endothelium: studies in cultured cells and smokers
    Gonzalo Peluffo
    Departmento de Bioquimica, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    Am J Physiol Heart Circ Physiol 296:H1781-92. 2009
    ..Our data indicate that elements on tobacco smoke, most likely through redox cycling, divert (*)NO toward peroxynitrite by inducing O(2)(*-) production in vascular endothelial cells both in vitro and in vivo...
  45. ncbi request reprint Inactivation and nitration of human superoxide dismutase (SOD) by fluxes of nitric oxide and superoxide
    Verónica Demicheli
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Avda Gral Flores 2125, 11800 Montevideo, Uruguay
    Free Radic Biol Med 42:1359-68. 2007
    ....
  46. ncbi request reprint Mitochondrial aconitase reaction with nitric oxide, S-nitrosoglutathione, and peroxynitrite: mechanisms and relative contributions to aconitase inactivation
    Verónica Tórtora
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    Free Radic Biol Med 42:1075-88. 2007
    ....
  47. ncbi request reprint Protein and lipid nitration: role in redox signaling and injury
    Homero Rubbo
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo Uruguay
    Biochim Biophys Acta 1780:1318-24. 2008
    ..e. to nitrofatty acids) results in the formation of novel endogenous modulators of redox processes, partially counteracting pro-inflammatory effects of oxidant exposure...
  48. ncbi request reprint Cytochrome c: a catalyst and target of nitrite-hydrogen peroxide-dependent protein nitration
    Laura Castro
    Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL 35233, USA
    Arch Biochem Biophys 421:99-107. 2004
    ..The data also infer that the distribution of specific proteins capable of serving as potent catalysts of nitration can lend both spatial and molecular specificity to biomolecule nitration reactions...
  49. ncbi request reprint Macrophage-derived peroxynitrite diffusion and toxicity to Trypanosoma cruzi
    María Noel Alvarez
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Arch Biochem Biophys 432:222-32. 2004
    ..cruzi during cell-to-cell contact and/or inside the phagosome...
  50. ncbi request reprint Reactions of desferrioxamine with peroxynitrite-derived carbonate and nitrogen dioxide radicals
    Silvina Bartesaghi
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 36:471-83. 2004
    ..The results shown herein provide an alternative mechanism to account for some of the biochemical and pharmacological actions of desferrioxamine via reactions with CO3*- and *NO2 radicals...
  51. ncbi request reprint Diffusion of nitric oxide into low density lipoprotein
    Ana Denicola
    Department of Physical Biochemistry, Facultad de Ciencias, Universidad de la Republica, 11400 Montevideo, Uruguay
    J Biol Chem 277:932-6. 2002
    ..Thus, our observations support that ( small middle dot)NO readily traverses the LDL surface accessing the hydrophobic lipid core of the particle and affirm a role for ( small middle dot)NO as a major lipophilic antioxidant in LDL...
  52. ncbi request reprint Reactions of manganese porphyrins with peroxynitrite and carbonate radical anion
    Gerardo Ferrer-Sueta
    Laboratorio de Fisicoquímica Biológica, Facultad de Ciencias, Universidad de la Republica, Montevideo 11400, Uruguay
    J Biol Chem 278:27432-8. 2003
    ....
  53. ncbi request reprint The mycobacterial thioredoxin peroxidase can act as a one-cysteine peroxiredoxin
    Madia Trujillo
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Avda General Flores 2125, UY 11800 Montevideo, Uruguay
    J Biol Chem 281:20555-66. 2006
    ..The role of Cys(93) is likely to conserve the oxidation equivalents of the sulfenic acid state of C(P) as a disulfide bond to prevent overoxidation of Cys(60) under a restricted supply of reducing substrate...
  54. ncbi request reprint Reaction of peroxynitrite with Mn-superoxide dismutase. Role of the metal center in decomposition kinetics and nitration
    C Quijano
    Departamento de Bioquimica, Facultad de Medicina, , 11800 Montevideo, Uruguay
    J Biol Chem 276:11631-8. 2001
    ..Our results indicate that the manganese ion in Mn-SOD plays an important role in the decomposition kinetics of peroxynitrite and in peroxynitrite-dependent nitration of self and remote tyrosine residues...
  55. ncbi request reprint Reactivity of sulfenic acid in human serum albumin
    Lucía Turell
    Laboratorios Enzimología, Facultad de Ciencias, Universidad de la Republica, Uruguay
    Biochemistry 47:358-67. 2008
    ..6 +/- 0.2, 2.9 +/- 0.5, 9.3 +/- 0.9, and 55 +/- 3 M-1 s-1 (25 degrees C, pH 7.4), respectively, supporting a role for HSA-SOH in the formation of mixed disulfides...
  56. ncbi request reprint Incorporation of the hydrophobic probe N-t-BOC-L-tyrosine tert-butyl ester to red blood cell membranes to study peroxynitrite-dependent reactions
    Natalia Romero
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Chem Res Toxicol 20:1638-48. 2007
    ....
  57. ncbi request reprint Peroxynitrite flux-mediated LDL oxidation is inhibited by manganese porphyrins in the presence of uric acid
    Andrés Trostchansky
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 35:1293-300. 2003
    ..These antioxidant effects should predominate under in vivo conditions having plasma uric acid concentration range between 150 and 500 microM...
  58. ncbi request reprint Peroxynitrite-mediated alpha-tocopherol oxidation in low-density lipoprotein: a mechanistic approach
    Horacio Botti
    Departamento de Bioqui mica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Biol Med 36:152-62. 2004
    ..NO and ascorbate may determine low alpha-tocopheryl quinone accumulation in tissues despite increased ONOO- generation...
  59. ncbi request reprint Kinetic studies on peroxynitrite reduction by peroxiredoxins
    Madia Trujillo
    Department of Biochemistry, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Methods Enzymol 441:173-96. 2008
    ..All methods reviewed provide reliable values of rate constants and a combination of them can be used to provide further reassurance; applicability and advantages of the different methodologies are discussed...
  60. pmc Mitochondrial superoxide radicals mediate programmed cell death in Trypanosoma cruzi: cytoprotective action of mitochondrial iron superoxide dismutase overexpression
    Lucia Piacenza
    Departamento de Bioquimica, Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochem J 403:323-34. 2007
    ....
  61. ncbi request reprint Hemoglobin and red blood cells as tools for studying peroxynitrite biochemistry
    Natalia Romero
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Methods Enzymol 396:229-45. 2005
    ....
  62. ncbi request reprint Formation and reactions of sulfenic acid in human serum albumin
    Beatriz Alvarez
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    Methods Enzymol 473:117-36. 2010
    ..Our results support the concept that sulfenic acid is a central intermediate in the formation of oxidized albumin species that are present in circulating albumin and increase under pathological conditions...
  63. pmc L-arginine-dependent suppression of apoptosis in Trypanosoma cruzi: contribution of the nitric oxide and polyamine pathways
    L Piacenza
    , Facultad de Medicina, , Avenida General Flores 2125, 11800, Montevideo, Uruguay
    Proc Natl Acad Sci U S A 98:7301-6. 2001
    ....
  64. doi request reprint Protein tyrosine nitration--functional alteration or just a biomarker?
    Jose M Souza
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Av Gral Flores 2125, Montevideo, Uruguay
    Free Radic Biol Med 45:357-66. 2008
    ..In this review, we analyze the factors determining protein 3-nitrotyrosine formation, the functional and biological outcome associated with protein tyrosine nitration, and the fate of the nitrated proteins...
  65. ncbi request reprint Peroxynitrite formation from biochemical and cellular fluxes of nitric oxide and superoxide
    María Noel Alvarez
    Department of Biochemistry, Universidad de la Republica, 11800 Montevideo, Uruguay
    Methods Enzymol 359:353-66. 2002
  66. doi request reprint Peroxynitrite detoxification and its biologic implications
    Madia Trujillo
    Departamento de Bioquimica, Universidad de la Republica, Montevideo, Uruguay
    Antioxid Redox Signal 10:1607-20. 2008
    ..Flavonoids, nitroxides, and tyrosine-containing peptides decreased peroxynitrite-mediated toxicity under different conditions, but their mechanism of action is indirect...
  67. doi request reprint Sulfenic acid--a key intermediate in albumin thiol oxidation
    Lucía Turell
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3384-92. 2009
    ....
  68. ncbi request reprint Antioxidant and diffusion properties of nitric oxide in low-density lipoprotein
    Homero Rubbo
    Department of Biochemistry, Universidad de la Republica, 11800 Montevideo, Uruguay
    Methods Enzymol 359:200-9. 2002
  69. ncbi request reprint Mechanistic studies of peroxynitrite-mediated tyrosine nitration in membranes using the hydrophobic probe N-t-BOC-L-tyrosine tert-butyl ester
    Silvina Bartesaghi
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo 11800, Uruguay
    Biochemistry 45:6813-25. 2006
    ..BTBE was successfully applied as a hydrophobic probe to study nitration mechanisms and will serve to study factors controlling protein and lipid nitration in biomembranes and lipoproteins...
  70. doi request reprint Dioxygen reactivity and heme redox potential of truncated human cystathionine beta-synthase
    Sebastián Carballal
    Laboratorio De Enzimologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay
    Biochemistry 47:3194-201. 2008
    ..Our results show that CBS may represent a previously unrecognized source of cytosolic superoxide radical...
  71. ncbi request reprint Direct measurement of nitric oxide and oxygen partitioning into liposomes and low density lipoprotein
    Matías Möller
    Laboratorio de Fisicoquímica Biológica, Facultad de Ciencias, Universidad de la Republica, 11400 Montevideo, Uruguay
    J Biol Chem 280:8850-4. 2005
    ....
  72. ncbi request reprint Biochemistry of protein tyrosine nitration in cardiovascular pathology
    Gonzalo Peluffo
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Cardiovasc Res 75:291-302. 2007
    ..Pharmacological strategies directed towards inhibiting protein nitration will assist to shed light on the relevance of this post-translational modification to human cardiovascular pathology...
  73. pmc Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite
    Lucia Piacenza
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochem J 410:359-68. 2008
    ..Together with recent reports of up-regulation of antioxidant enzymes during metacyclogenesis, our results identify components of the antioxidant enzyme network of T. cruzi as virulence factors of emerging importance...
  74. ncbi request reprint Metabolic control analysis of mitochondrial aconitase: influence over respiration and mitochondrial superoxide and hydrogen peroxide production
    F Scandroglio
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Free Radic Res 48:684-93. 2014
    ....
  75. ncbi request reprint Peroxynitrite and drug-dependent toxicity
    Ana Denicola
    Lab Fisicoquímica Biológica, Facultad de Ciencias, Universidad de la Republica, Igua 4225, 11400 Montevideo, Uruguay
    Toxicology 208:273-88. 2005
    ....
  76. doi request reprint Mitochondrial calcium overload triggers complement-dependent superoxide-mediated programmed cell death in Trypanosoma cruzi
    Florencia Irigoín
    Departamento de Histologia y Embriologia, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochem J 418:595-604. 2009
    ..Thus our results suggest a model in which MAC assembly on the parasite surface allows Ca(2+) entry and its accumulation in the mitochondrion, leading to O(2)(*-) production, which in turn constitutes a PCD signal...
  77. doi request reprint Insights into the redox biology of Trypanosoma cruzi: Trypanothione metabolism and oxidant detoxification
    Florencia Irigoín
    Departmento de Histología y Embriología, Facultad de Medicina, Universidad de la Republica, Uruguay
    Free Radic Biol Med 45:733-42. 2008
    ..Together, the evidence suggests a central role of the trypanothione-dependent antioxidant systems in the infection process...
  78. ncbi request reprint Design, synthesis, and biological characterization of potential antiatherogenic nitric oxide releasing tocopherol analogs
    Gloria V Lopez
    Departamento de Quimica Organica, Facultad de Ciencias Facultad de Química, Universidad de la Republica, Montevideo, Uruguay
    Bioorg Med Chem 13:5787-96. 2005
    ..The selected NO-donor moieties were nitrooxy and furoxan. All products were tested for their in vitro NO-releasing capacities, vasodilating properties, and antiplatelet activity. They were also capable of preventing LDL oxidation...
  79. ncbi request reprint Nitrocytochrome c: synthesis, purification, and functional studies
    Jose M Souza
    Department of Biochemistry, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Methods Enzymol 441:197-215. 2008
    ....
  80. doi request reprint Fighting the oxidative assault: the Trypanosoma cruzi journey to infection
    Lucia Piacenza
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Montevideo Uruguay, Uruguay
    Curr Opin Microbiol 12:415-21. 2009
    ..The potency of the parasite antioxidant armamentarium influences the final fate of the Trypanosoma cruzi journey to macrophage invasion at the onset of infection...
  81. ncbi request reprint Prevention of peroxynitrite-induced apoptosis of motor neurons and PC12 cells by tyrosine-containing peptides
    Yaozu Ye
    Burke Medical Research Institute, White Plains, New York 10605, USA
    J Biol Chem 282:6324-37. 2007
    ....
  82. ncbi request reprint Plasmodium falciparum 2-Cys peroxiredoxin reacts with plasmoredoxin and peroxynitrite
    Christine Nickel
    Interdisciplinary Research Centre, Justus Liebig University, D 35392 Giessen, Germany
    Biol Chem 386:1129-36. 2005
    ....
  83. ncbi request reprint Cyclic guanosine 5' monophosphate (GMP) prevents expression of neuronal nitric oxide synthase and apoptosis in motor neurons deprived of trophic factors in rats
    ALVARO G ESTEVEZ
    Department of Physiology and Biophysics, University of Alabama at Birmingham, 1918 University Boulevard, MCLM 850, Birmingham, AL 35294 0005, USA
    Neurosci Lett 326:201-5. 2002
    ..These results suggest that elevation of cGMP could decrease nitric oxide production, and thereby preventing motor neuron apoptosis...
  84. ncbi request reprint Albumin and hydroxyethyl starch modulate oxidative inflammatory injury to vascular endothelium
    John D Lang
    Department of Anesthesiology and Center for Free Radical Biology, The University of Alabama at Birmingham, 35233 6810, USA
    Anesthesiology 100:51-8. 2004
    ....
  85. ncbi request reprint EPR detection of glutathiyl and hemoglobin-cysteinyl radicals during the interaction of peroxynitrite with human erythrocytes
    Ohara Augusto
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Brazil
    Biochemistry 41:14323-8. 2002
    ..Overall, the results indicate that, through the intermediacy of carbon dioxide and/or hemoproteins, oxidation of glutathione to the glutathiyl radical is likely to be an important consequence of peroxynitrite production in vivo...
  86. ncbi request reprint Mitochondrial superoxide production and nuclear factor erythroid 2-related factor 2 activation in p75 neurotrophin receptor-induced motor neuron apoptosis
    Mariana Pehar
    Departamento de Neurobiologia Celular y Molecular, Instituto de Investigaciones Biologicas Clemente Estable, Montevideo 11600, Uruguay
    J Neurosci 27:7777-85. 2007
    ..This apoptotic pathway is facilitated by the expression of ALS-linked SOD1 mutations and critically modulated by Nrf2 activity...
  87. pmc Nitro-fatty acid reaction with glutathione and cysteine. Kinetic analysis of thiol alkylation by a Michael addition reaction
    Laura M S Baker
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 282:31085-93. 2007
    ..In aggregate, these results reveal that the reversible adduction of thiols by nitro-fatty acids is a mechanism for reversible post-translational regulation of protein function by nitro-fatty acids...
  88. doi request reprint Peroxynitrite inhibits electron transport on the acceptor side of higher plant photosystem II
    Sergio González-Pérez
    Departamento de Estrés Abiótico, Instituto de Recursos Naturales y Agrobiología de Salamanca IRNASA CSIC, Apdo 257, 37071 Salamanca, Spain
    Arch Biochem Biophys 473:25-33. 2008
    ..This new EPR signal corresponds with the semireduced plastoquinone Q(A) in the absence of magnetic interaction with the non-heme Fe2+. We conclude that peroxynitrite impairs PSII electron transport in the Q(A)Fe2+ niche...
  89. ncbi request reprint Formation of protein tyrosine ortho-semiquinone radical and nitrotyrosine from cytochrome c-derived tyrosyl radical
    Yeong Renn Chen
    Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:18054-62. 2004
    ..The results of this study may provide useful information in understanding the interconnection among cyt c, H(2)O(2), NO, and apoptosis...