Andriy A Sibirny

Summary

Affiliation: Institute of Cell Biology
Country: Ukraine

Publications

  1. pmc Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1
    Vira M Ubiyvovk
    Institute of Cell Biology NAS of Ukraine, Drahomanov Street, 14 16, Lviv, 79005 Ukraine
    BMC Biotechnol 11:8. 2011
  2. doi request reprint Mechanisms of autophagy and pexophagy in yeasts
    A A Sibirny
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv 79005, Ukraine
    Biochemistry (Mosc) 76:1279-90. 2011
  3. doi request reprint Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose
    Olena P Ishchuk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    FEMS Yeast Res 8:1164-74. 2008
  4. ncbi request reprint Differences in glucose sensing and signaling for pexophagy between the baker's yeast Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris
    Volodymyr Y Nazarko
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    Autophagy 4:381-4. 2008
  5. pmc Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris
    Volodymyr Y Nazarko
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    Autophagy 7:375-85. 2011
  6. doi request reprint Gss1 protein of the methylotrophic yeast Pichia pastoris is involved in glucose sensing, pexophagy and catabolite repression
    Andriy S Polupanov
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine
    Int J Biochem Cell Biol 44:1906-18. 2012
  7. pmc Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis
    Yuriy R Boretsky
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
    FEMS Yeast Res 11:307-14. 2011
  8. doi request reprint Construction of Hansenula polymorpha strains with improved thermotolerance
    Olena P Ishchuk
    Institute of Cell Biology, NAS of Ukraine, Lviv 79005, Ukraine
    Biotechnol Bioeng 104:911-9. 2009
  9. pmc Deficiency in frataxin homologue YFH1 in the yeast Pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation
    Yuriy V Pynyaha
    Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, 79005 Lviv, Ukraine
    Biometals 22:1051-61. 2009
  10. doi request reprint Heterologous expression of Saccharomyces cerevisiae MPR1 gene confers tolerance to ethanol and L: -azetidine-2-carboxylic acid in Hansenula polymorpha
    Olena P Ishchuk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    J Ind Microbiol Biotechnol 37:213-8. 2010

Collaborators

  • Martin Zimmermann
  • Kostyantyn V Dmytruk
  • Caroline C Philpott
  • Suresh Subramani
  • Wolfgang Schuhmann
  • Patrick Van Dijck
  • Oleh V Stasyk
  • Volodymyr Y Nazarko
  • Olena P Ishchuk
  • Andriy Y Voronovsky
  • Charles A Abbas
  • Yuriy R Boretsky
  • Vira M Ubiyvovk
  • Olena G Stasyk
  • Andriy S Polupanov
  • Yuriy V Pynyaha
  • Olena V Dmytruk
  • Daria V Fedorovych
  • Johan M Thevelein
  • Olena S Krasovska
  • Oleksandra V Blazhenko
  • Yaroslav P Bobak
  • Taras Y Nazarko
  • Volodymyr Y Boretsky
  • Valentyna Y Yatsyshyn
  • Jean Claude Farre
  • James M Cregg
  • Hyun Ah Kang
  • Olga V Protchenko
  • Dariya V Fedorovych
  • Dirk Warnecke
  • Olha Protchenko
  • Lyubov R Fayura
  • Alexander Y Malyshev
  • Stanislaw Ulaszewski
  • Vladimir M Ananin
  • Yuliya V Kurlishchuk
  • Bozhena O Vynnytska
  • Olha V Rohulya
  • Olha V Protchenko
  • Lubov R Fayura
  • Andriy I Levkiv
  • Galina Z Gayda
  • Mykhailo V Gonchar
  • Kateryna O Futej
  • Mykola M Maidan
  • Igor O Mukalov
  • Oleksandr F Vozianov
  • Nikolai Granovski
  • Marten Veenhuis
  • Huyn Ah Kang
  • Tetiana M Prokopiv
  • Viktor O Nahorny
  • Vasyl I Kutsyaba
  • Tim van Zutphen
  • Vitaliy A Kordium
  • Michel J Penninckx
  • Richard D Mathewson
  • Daniel Gigot
  • Grzegorz Bartosz
  • Michel Penninckx
  • Olena B Ryabova
  • Olena V Verba

Detail Information

Publications33

  1. pmc Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1
    Vira M Ubiyvovk
    Institute of Cell Biology NAS of Ukraine, Drahomanov Street, 14 16, Lviv, 79005 Ukraine
    BMC Biotechnol 11:8. 2011
    ..Cellular and extracellular glutathione production of H. polymorpha DL-1 in the wild type and recombinant strains which overexpress genes of glutathione biosynthesis (GSH2) and its precursor cysteine (MET4) was studied...
  2. doi request reprint Mechanisms of autophagy and pexophagy in yeasts
    A A Sibirny
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv 79005, Ukraine
    Biochemistry (Mosc) 76:1279-90. 2011
    ....
  3. doi request reprint Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose
    Olena P Ishchuk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    FEMS Yeast Res 8:1164-74. 2008
    ..The introduction of multiple copies of the H. polymorpha PDC1 gene driven by the strong constitutive promoter led to a 20-fold increase in pyruvate decarboxylase activity and up to a threefold elevation of ethanol production...
  4. ncbi request reprint Differences in glucose sensing and signaling for pexophagy between the baker's yeast Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris
    Volodymyr Y Nazarko
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    Autophagy 4:381-4. 2008
    ..This implies that glucose sensing for pexophagy is different in baker's and methylotrophic yeasts...
  5. pmc Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris
    Volodymyr Y Nazarko
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    Autophagy 7:375-85. 2011
    ..In summary, Atg35 is a new Atg protein that relocates to the PNS and specifically regulates MIPA formation during micropexophagy...
  6. doi request reprint Gss1 protein of the methylotrophic yeast Pichia pastoris is involved in glucose sensing, pexophagy and catabolite repression
    Andriy S Polupanov
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine
    Int J Biochem Cell Biol 44:1906-18. 2012
    ..GSS1 knock-out strain has been constructed. The experiments support the hypothesis that Gss1 plays an important role in autophagic degradation of peroxisomes and glucose catabolite repression in P. pastoris...
  7. pmc Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis
    Yuriy R Boretsky
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
    FEMS Yeast Res 11:307-14. 2011
    ..Complementation analysis revealed that Δvma1-17 and Δfra1-45 mutants are distinct from previously reported riboflavin-producing mutants hit1-1, rib80-22 and rib81-31 of this yeast...
  8. doi request reprint Construction of Hansenula polymorpha strains with improved thermotolerance
    Olena P Ishchuk
    Institute of Cell Biology, NAS of Ukraine, Lviv 79005, Ukraine
    Biotechnol Bioeng 104:911-9. 2009
    ..9 g L(-1), our model H. polymorpha strains with elevated thermotolerance can be further modified by metabolic engineering to construct improved high-temperature ethanol producers from this pentose...
  9. pmc Deficiency in frataxin homologue YFH1 in the yeast Pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation
    Yuriy V Pynyaha
    Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, 79005 Lviv, Ukraine
    Biometals 22:1051-61. 2009
    ..Thus, deletion of the gene coding for yeast frataxin homologue has pleiotropic effect on metabolism in P. guilliermondii...
  10. doi request reprint Heterologous expression of Saccharomyces cerevisiae MPR1 gene confers tolerance to ethanol and L: -azetidine-2-carboxylic acid in Hansenula polymorpha
    Olena P Ishchuk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    J Ind Microbiol Biotechnol 37:213-8. 2010
    ..The obtained results suggest that the expression of the S. cerevisiae MPR1 gene in H. polymorpha can be a useful approach in the construction of H. polymorpha strains with improved ethanol resistance...
  11. ncbi request reprint The role of Hansenula polymorpha MIG1 homologues in catabolite repression and pexophagy
    Olena G Stasyk
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
    FEMS Yeast Res 7:1103-13. 2007
    ..Our findings suggest that homologues of the elements of the Saccharomyces cerevisiae main repression pathway have pleiotropic functions in H. polymorpha...
  12. doi request reprint CCZ1, MON1 and YPT7 genes are involved in pexophagy, the Cvt pathway and non-specific macroautophagy in the methylotrophic yeast Pichia pastoris
    Andriy S Polupanov
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine
    Cell Biol Int 35:311-9. 2011
    ..The data suggest that CCZ1, MON1 and YPT7 genes are involved in degradation of both small oleate-induced and large methanol-induced peroxisomes. The data suggest conservative functions of CCZ1, MON1 and YPT7 genes among yeast species...
  13. pmc Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha
    Olena G Stasyk
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St 14 16, Lviv 79005, Ukraine
    Eukaryot Cell 7:735-46. 2008
    ..polymorpha, the single transporter-like sensor Hxs1 mediates signaling in the hexose induction pathway, whereas the rate of hexose uptake affects the strength of catabolite repression...
  14. doi request reprint Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan
    Andriy Y Voronovsky
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv 79005, Ukraine
    Metab Eng 11:234-42. 2009
    ..Resulting transformants were capable of growth and alcoholic fermentation on a minimal medium supplemented with birchwood xylan as a sole carbon source at 48 degrees C...
  15. doi request reprint Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production
    Kostyantyn V Dmytruk
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, Lviv 79005, Ukraine
    Metab Eng 13:82-8. 2011
    ..D. hansenii IMH3 and modified ARO4 genes conferring resistance to mycophenolic acid and fluorophenylalanine, respectively, were successfully used as new dominant selection markers for C. famata...
  16. pmc Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor
    Kostyantyn V Dmytruk
    Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, Lviv 79005, Ukraine
    BMC Biotechnol 11:58. 2011
    ..The fabrication of cheap and reliable urate-selective amperometric biosensors is a challenging task...
  17. ncbi request reprint Mutations and environmental factors affecting regulation of riboflavin synthesis and iron assimilation also cause oxidative stress in the yeast Pichia guilliermondii
    Yuriy R Boretsky
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
    J Basic Microbiol 47:371-7. 2007
    ..The data suggest that the regulation of riboflavin biosynthesis and iron assimilation in P. guilliermondii are linked with cellular oxidative state...
  18. doi request reprint Methods of plate pexophagy monitoring and positive selection for ATG gene cloning in yeasts
    Oleh V Stasyk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    Methods Enzymol 451:229-39. 2008
    ..These methods were successfully utilized in several methylotrophic yeasts and the alkane-utilizing yeast Yarrowia lipolytica...
  19. doi request reprint Candida famata (Candida flareri)
    Kostyantyn V Dmytruk
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences, Lviv, Ukraine
    Yeast 29:453-8. 2012
    ..The construction of improved yeast strains producing riboflavin, FMN and FAD, based on the industrial riboflavin-producing strain dep8 and its non-reverting analogue AF4, is also described...
  20. ncbi request reprint Overexpression of bacterial xylose isomerase and yeast host xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha
    Olena V Dmytruk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    FEMS Yeast Res 8:165-73. 2008
    ..Xylulokinase activities of the transformants increased twofold when compared with the parental strain. The recombinant strains displayed improved ethanol production during the fermentation of xylose...
  21. pmc Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers
    Charles A Abbas
    Archer Daniels Midland Company, Decatur, Illinois 625261, USA
    Microbiol Mol Biol Rev 75:321-60. 2011
    ....
  22. doi request reprint Production of flavin mononucleotide by metabolically engineered yeast Candida famata
    Valentyna Y Yatsyshyn
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street, 14 16, Lviv 79005, Ukraine
    Metab Eng 11:163-7. 2009
    ..The obtained C. famata recombinant strains can be used for the further construction of improved FMN overproducers...
  23. ncbi request reprint Glucose-induced production of recombinant proteins in Hansenula polymorpha mutants deficient in catabolite repression
    Olena S Krasovska
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street 14 16, Lviv 79005, Ukraine
    Biotechnol Bioeng 97:858-70. 2007
    ..The modified expression platform preserves the favorable regulatable nature of P(MOX) without methanol, making a convenient alternative to the traditional system...
  24. pmc Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha
    Olena V Dmytruk
    Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, Lviv 79005, Ukraine
    Microb Cell Fact 7:21. 2008
    ..abstract:..
  25. ncbi request reprint Role of gamma-glutamyltranspeptidase in detoxification of xenobiotics in the yeasts Hansenula polymorpha and Saccharomyces cerevisiae
    Vira M Ubiyvovk
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street, 14 16, Lviv 79005 Ukraine
    Cell Biol Int 30:665-71. 2006
    ..cerevisiae occurs through a gammaGT-dependent mercapturic acid pathway of GSH-xenobiotic detoxification, similar to that known for mammalian cells, with cysteine-xenobiotics and/or N-acetylcysteine-xenobiotics as the end products...
  26. ncbi request reprint G-protein-coupled receptor Gpr1 and G-protein Gpa2 of cAMP-dependent signaling pathway are involved in glucose-induced pexophagy in the yeast Saccharomyces cerevisiae
    Volodymyr Y Nazarko
    Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14 16, Lviv 79005, Ukraine
    Cell Biol Int 32:502-4. 2008
    ..We conclude that proteins Gpr1 and Gpa2 are involved in glucose sensing and signal transduction during pexophagy process in yeast...
  27. pmc Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor
    Kostyantyn V Dmytruk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    BMC Biotechnol 7:33. 2007
    ..Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming procedure of sample dilution...
  28. ncbi request reprint Development of a transformation system for gene knock-out in the flavinogenic yeast Pichia guilliermondii
    Yuriy R Boretsky
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology NAS of Ukraine, Dragomanov str 14 16, Lviv 79005, Ukraine
    J Microbiol Methods 70:13-9. 2007
    ..Efficient gene deletion in P. guilliermondii can be achieved using the modified URA3 gene of S. cerevisiae flanked by 0.8-0.9 kb sequences homologous to the target gene...
  29. ncbi request reprint Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments
    Kostyantyn V Dmytruk
    Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine
    Curr Genet 50:183-91. 2006
    ..famata. The results obtained indicate that insertional mutagenesis is a powerful tool for tagging genes in C. famata...
  30. ncbi request reprint Accumulation of cadmium ions in the methylotrophic yeast Hansenula polymorpha
    Oleksandra V Blazhenko
    Institute of Cell Biology, Nat Acad Sci of Ukraine, Drahomanov Str 14 16, Lviv, 79005, Ukraine
    Biometals 19:593-9. 2006
    ..We suggest that MgATP dependent transporter involved in Cd-GSH uptake in H. polymorpha, is similar to S. cerevisiae Ycf1-mediated vacuolar transporter responsible for accumulation of organic GS-conjugates and Cd-GSH complex...
  31. ncbi request reprint Atg28, a novel coiled-coil protein involved in autophagic degradation of peroxisomes in the methylotrophic yeast Pichia pastoris
    Oleh V Stasyk
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
    Autophagy 2:30-8. 2006
    ..The identification of ATG28 suggests that pexophagy may involve species-specific components, since this gene appears to have only weak homologues in other yeasts...
  32. doi request reprint Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes
    Yaroslav P Bobak
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
    Cell Biol Int 34:1085-9. 2010
    ..We also observed that OTC expression was below the level of detection in all the types of tumour cells analysed, suggesting that in vitro, at least for them, Arg is an essential amino acid...
  33. ncbi request reprint Expression of xylA genes encoding xylose isomerases from Escherichia coli and Streptomyces coelicolor in the methylotrophic yeast Hansenula polymorpha
    Andriy Y Voronovsky
    Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, Lviv 79005, Ukraine
    FEMS Yeast Res 5:1055-62. 2005
    ..The transformants did not differ from the yeast wild-type strain with respect to ethanol production in xylose medium...