Alan D Winter
- Prolyl 4-hydroxlase activity is essential for development and cuticle formation in the human infective parasitic nematode Brugia malayiAlan D Winter
Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Garscube Estate, Bearsden Road, Glasgow G61 1QH, Scotland, United Kingdom
J Biol Chem 288:1750-61. 2013..We therefore demonstrate that C-P4H activity is essential for development of B. malayi and uncover a novel parasite-specific feature of these collagen biosynthetic enzymes that may be exploited in future parasite control...
- Diversity in parasitic nematode genomes: the microRNAs of Brugia pahangi and Haemonchus contortus are largely novelAlan D Winter
Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences University of Glasgow, Garscube Estate, Bearsden Road, Glasgow, G61 1QH, UK
BMC Genomics 13:4. 2012..Here we describe the the miRNA repertoire of two important parasitic nematodes as an essential first step in addressing this question...
- Combined extracellular matrix cross-linking activity of the peroxidase MLT-7 and the dual oxidase BLI-3 is critical for post-embryonic viability in Caenorhabditis elegansMelanie C Thein
Institute of Comparative Medicine, Veterinary Faculty, University of Glasgow, Glasgow, Scotland, UK
J Biol Chem 284:17549-63. 2009..This points toward a functionally cooperative relationship for these two hypodermally expressed proteins that is essential for collagen cross-linking and proper extracellular matrix formation...
- Protein disulfide isomerase activity is essential for viability and extracellular matrix formation in the nematode Caenorhabditis elegansAlan D Winter
Institute of Comparative Medicine, Veterinary Faculty, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, Scotland, UK
Dev Biol 308:449-61. 2007..PDI-2 therefore performs two critical roles during morphogenesis. The role of PDI-2 in collagen biogenesis can be restored following complementation of the mutant with human PDI...
- Differences in collagen prolyl 4-hydroxylase assembly between two Caenorhabditis nematode species despite high amino acid sequence identity of the enzyme subunitsAlan D Winter
Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road Glasgow, G61 1QH, Scotland, United Kingdom
Matrix Biol 26:382-95. 2007..Genetic complementation of the C. briggsae and C. elegans phy-1 mutants was achieved by injection of a wild type phy-1 gene from either species...
- Loss of secretory pathway FK506-binding proteins results in cold-sensitive lethality and associate extracellular matrix defects in the nematode Caenorhabditis elegansAlan D Winter
Institute of Comparative Medicine, Faculty of Veterinary Medicine, The University of Glasgow, Bearsden Road, Glasgow G61 1QH, Scotland, United Kingdom
J Biol Chem 282:12813-21. 2007..This study establishes that the secretory pathway FK506-binding PPIase enzymes are essential for normal nematode development, collagen biogenesis, and the formation of an intact exoskeleton under adverse physiological conditions...
- Caenorhabditis elegans exoskeleton collagen COL-19: an adult-specific marker for collagen modification and assembly, and the analysis of organismal morphologyMelanie C Thein
Wellcome Centre for Molecular Parasitology, Anderson College, The University of Glasgow, Glasgow, United Kingdom
Dev Dyn 226:523-39. 2003....
- A highly conserved, inhibitable astacin metalloprotease from Teladorsagia circumcincta is required for cuticle formation and nematode developmentGillian Stepek
Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK
Int J Parasitol 45:345-55. 2015..These compounds correspondingly inhibited the activity of recombinant T. circumcincta DPY-31, supporting the hypothesis that this enzyme may represent a potentially novel anthelmintic drug target. ..
- A hypodermally expressed prolyl 4-hydroxylase from the filarial nematode Brugia malayi is soluble and active in the absence of protein disulfide isomeraseAlan D Winter
Wellcome Centre for Molecular Parasitology, Anderson College, University of Glasgow, Scotland, United Kingdom
J Biol Chem 278:2554-62. 2003..elegans. Although active by itself, the B. malayi PHY-1 was not able to replace enzyme function in a C. elegans P4H mutant...
- microRNAs: a role in drug resistance in parasitic nematodes?Eileen Devaney
Parasitology Group, Division of Veterinary Infection and Immunity, Institute for Comparative Medicine, School of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK
Trends Parasitol 26:428-33. 2010..microRNAs play an important role in resistance to chemotherapeutic agents in many tumour cells and here we discuss whether they might also be involved in anthelmintic resistance in parasitic nematodes...
- Application of small RNA technology for improved control of parasitic helminthsCollette Britton
Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK Electronic address
Vet Parasitol 212:47-53. 2015..In this review we focus on the important roles of miRNAs and siRNAs in post-transcriptional gene regulation in veterinary parasitic helminths and the potential value of these in parasite diagnosis and control. ..