Research Topics
Genomes and GenesSpecies | Wei WangSummaryAffiliation: Wellcome Trust Genome Campus Country: UK Publications
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Detail Information
Publications
A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cellsWei Wang
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, PR China
Genome Biol 8:R48. 2007..In order to identify the host factors involved in retroviral infection, we designed and implemented a scheme for identifying ES cells that are resistant to retroviral infection and subsequent cloning of the mutated gene...
Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expressionYuchun Nie
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China
Biochem Biophys Res Commun 321:994-1000. 2004..In addition, we also demonstrated angiotensin 1 converting enzyme (ACE)-the homologue of ACE2 could not function as a receptor for SARS-CoV...- Neutralizing antibodies in patients with severe acute respiratory syndrome-associated coronavirus infectionYuchun Nie
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China
J Infect Dis 190:1119-26. 2004..These results suggest that it is possible to develop effective vaccines against SARS and that NAbs provide a potential strategy for treating patients with SARS... - A novel variant lattice corneal dystrophy caused by association of mutation (V625D) in TGFBI geneXin Tian
Department of Ophthalmology, Third Hospital, Peking University, Beijing, China
Am J Ophthalmol 144:473-5. 2007..To characterize the molecular defect in the TGFBI gene in a Chinese family affected with an atypical lattice corneal dystrophy... - New triterpenoids from Kadsura heteroclita and their cytotoxic activityWei Wang
School of Pharmaceutical Sciences, Peking University, Beijing, People s Republic of China
Planta Med 72:450-7. 2006..Among the compounds tested, heteroclitalactone D (4) showed the strongest cytotoxic activity against the HL-60 cells with an IC50 of 6.76 microM... - Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cellsLing Yi
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, Peoples Republic of China
J Virol 78:11334-9. 2004.... 
Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobaccoFeng Li
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai an, Shandong 271018, PR China
J Plant Physiol 168:960-6. 2011....- Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoproteinKehu Yuan
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China
Biochem Biophys Res Commun 319:746-52. 2004..HR1-1 and HR2-18 can serve as functional probes for dissecting the fusion mechanism of SARS-CoV and also provide the potential of further identifying potent inhibitors for SARS-CoV entry... - Two prenylated and C-methylated flavonoids from Tripterygium wilfordiiFeng Zeng
School of Pharmaceutical Sciences, Peking University, Beijing, People s Republic of China
Planta Med 76:1596-9. 2010..0 ± 0.7 µM. Both compounds showed moderate antiproliferative activity against the tumor cell lines HT-29 and ZR-75-1... - Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodiesHong Zhang
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China
J Virol 78:6938-45. 2004..Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV... - Construction of armored RNA containing long-size chimeric RNA by increasing the number and affinity of the pac site in exogenous rna and sequence coding coat protein of the MS2 bacteriophageBaojun Wei
Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, National Center for Clinical Laboratory, Beijing Hospital, Beijing, PR China
Intervirology 51:144-50. 2008..To construct a one-plasmid expression system of the armored RNA containing long chimeric RNA by increasing the number and affinity of the pac site... - Mechanosensitivity of STREX-lacking BKCa channels in the colonic smooth muscle of the mouseWei Wang
Dept of Physiology, Capital Medical Univ, Beijing, PR China
Am J Physiol Gastrointest Liver Physiol 299:G1231-40. 2010..We suggest that in addition to the STREX domain, there are other additional structures in the channel responsible for mechanically coupling with the cell membrane... - Amperometric hydrogen peroxide biosensor based on the immobilization of heme proteins on gold nanoparticles-bacteria cellulose nanofibers nanocompositeWei Wang
Beijing National Laboratory for Molecular Sciences BNLMS, Key Laboratory of Biochemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, PR China
Talanta 84:71-7. 2011..3 μM to 1.00 mM, and a low detection limit of 0.1 μM based on S/N=3. The high performance of the biosensor made Au-BC nanocomposite superior to other materials as immobilization matrix... - A laminar flow electroporation system for efficient DNA and siRNA deliveryZewen Wei
National Key Laboratory of Science and Technology on Micro Nano Fabrication, Institute of Microelectronics, Peking University, Beijing, People s Republic of China
Anal Chem 83:5881-7. 2011..Furthermore, successful electrotransfection of plasmid DNA and, more importantly, synthetic siRNA, was demonstrated in several hard-to-transfect cell types using this system... - Low molecular weight heparin ablates lung cancer cisplatin-resistance by inducing proteasome-mediated ABCG2 protein degradationQi Niu
Department of Medical Oncology, No 309 PLA Hospital, Beijing, PR China
PLoS ONE 7:e41035. 2012..This study provides an experimental basis for using a combination of LMWH, which targets lung SP cells, with chemotherapy to improve lung cancer survival... - The effect of Acorus gramineus on the bioavailabilities and brain concentrations of ginsenosides Rg1, Re and Rb1 after oral administration of Kai-Xin-San preparations in ratsWei Wang
Institute of Medicinal Plant Development IMPLAD, Chinese Academy of Medical Sciences and Peking Union Medical College, Haidian District, Beijing, PR China
J Ethnopharmacol 131:313-20. 2010.... - Studies on Parylene C-caulked PDMS (pcPDMS) for low permeability required microfluidics applicationsYinhua Lei
National Key Laboratory of Science and Technology on Micro Nano Fabrication, Institute of Microelectronics, Peking University, Beijing, PR China
Lab Chip 11:1385-8. 2011..The bonding issue enrolled in the pcPDMS fabrication was solved based on careful surface analyses of the Parylene C caulked status in the PDMS matrix... - A new chiratane type triterpenoid from the rhizomes of Drynaria fortuneiYong Hong Liang
School of Pharmaceutical Sciences, Peking University, Beijing, PR China
Fitoterapia 81:988-91. 2010..Primary bioassays showed that compound 1 had significant cytotoxic activity against Hela, PC3 and HepG2 cells, with IC(50) of 2.92 μM, 1.08 μM and 2.45 μM, respectively... - Pseudo-homogeneous immunoextraction of epitestosterone from human urine samples based on gold-coated magnetic nanoparticlesShuang Qiu
Beijing National Laboratory for Molecular Sciences BNLMS, Key Laboratory of Biochemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry, Peking University, Beijing, China
Talanta 81:819-23. 2010..Compared with the conventional technique using immunoaffinity column, the method described here for sample pretreatment was fast, highly specific, and easy to operate... - N-[2-chloro-9H-purin-6-yl]-N-cyclopropylglycylamino acids and derivatives: synthesis, evaluation as a class of novel analgesics, and 3D QSAR analysisJinghua Zhao
College of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China
Bioorg Med Chem 17:6305-10. 2009..923, and 0.852, respectively. Using this equation pain threshold variations of 9 compounds were predicted and the errors ranged from 1.71 to 8.92... - Generation of monoclonal antibodies against highly conserved antigensHongzhe Zhou
Center of Infection and Immunity, National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, PR China
PLoS ONE 4:e6087. 2009..However, due to immune tolerance, some proteins that are highly conserved between mice and humans are not very immunogenic in mice, making it difficult to generate antibodies using a conventional approach... - Inhibition of HCV RNA-dependent RNA polymerase activity by aqueous extract from Fructus Ligustri LucidiLingbao Kong
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, PR China
Virus Res 128:9-17. 2007..Taken together, these results indicated that Fructus Ligustri Lucidi might offer a promising source of antiviral drugs against HCV NS5B. Purification of the active compound(s) and antiviral effect are clearly required in the future... - Characterization of phenolic compounds in the crude extract of Hedysarum multijugum by high-performance liquid chromatography with electrospray ionization tandem mass spectrometryMin Yang
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China
Rapid Commun Mass Spectrom 21:3833-41. 2007..The method established in this study could be applied to the comprehensive quality control of the herb and its formulations. It could also be applied to the biological and pharmacological research of traditional Chinese medicines... - Prevalence and factors associated with hepatitis B immunization and infection among men who have sex with men in Beijing, ChinaChao Wang
School of Public Health and Family Medicine, Capital Medical University, Beijing, China
PLoS ONE 7:e48219. 2012..Men who have sex with men (MSM) are at increased risk for HBV infection; however, the prevalence of HBV immunization and infection among Chinese MSM remains undetermined... - DEDD interacts with PI3KC3 to activate autophagy and attenuate epithelial-mesenchymal transition in human breast cancerQi Lv
Molecular Immunology and Cancer Pharmacology Groups, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China
Cancer Res 72:3238-50. 2012..DEDD expression therefore may represent a prognostic marker and potential therapeutic target for the prevention and treatment of cancer metastasis... - [Significance of phosphoinositide 3 kinase/AKT pathway alterations in endometrial carcinoma]Xi Yang
Department of Gynecology and Obstetrics, First Hospital, Peking University, Beijing, China
Zhonghua Bing Li Xue Za Zhi 40:799-804. 2011..To investigate the clinicopathologic and prognostic implications of phosphoinositide 3 kinase (PI3K)/AKT pathway alterations in endometrial cancers of Chinese women... - Synthesis and cytotoxic activities of beta-carboline amino acid ester conjugatesMing Zhao
College of Pharmaceutical Sciences, Capital University of Medical Sciences, Beijing, 100054, PR China
Bioorg Med Chem 14:6998-7010. 2006.... - Novel mutation (V505D) of the TGFBI gene found in a Chinese family with lattice corneal dystrophy, type IXin Tian
Department of Ophthalmology, Third Hospital, Peking University, Beijing, China
Jpn J Ophthalmol 49:84-8. 2005..To report a novel V505D mutation of the human transforming growth factor beta-induced (TGFBI) gene found in a Chinese family with lattice corneal dystrophy, type I (LCDI)... - Inhibitory effect of aqueous Dandelion extract on HIV-1 replication and reverse transcriptase activityHuamin Han
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China
BMC Complement Altern Med 11:112. 2011..In this study, we aimed to investigate the anti-HIV-1 activity of aqueous dandelion extract... - Association between dopaminergic genes (SLC6A3 and DRD2) and stuttering among Han ChineseJie Lan
Department of Genetics, College of Life Sciences, Graduate University of Chinese Academy of Sciences, Beijing, PR China
J Hum Genet 54:457-60. 2009..The results show that the presence of C allele at rs6277 in DRD2 gene is associated with increased susceptibility to the disorder, whereas T allele is protective. Haplotype 939T/957T is also a protective factor... - Bacterial expression and purification of biologically active human TFF3Min Fang
Department of Biochemistry and Molecular Biology, National Laboratory of Protein Engineering, College of Life Science, Peking University, Beijing, PR China
Peptides 25:785-92. 2004..Oral administration of recombinant hTFF3 has a dose dependent protective effect against ethanol-induced or pylorus ligation-induced gastric mucosa injury in rat, which indicates that our recombinant hTFF3 is biologically active... - A microfluidics cytometer for mice anemia detectionYanrui Ju
National Key Laboratory of Science and Technology on Nano Micro Fabrication Technology, Institute of Microelectronics, Peking University, Beijing, 100871, China
Lab Chip 12:4355-62. 2012.... - Kinetic difference of baicalin in rat blood and cerebral nuclei after intravenous administration of Scutellariae Radix extractLujun Zhang
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, PR China
J Ethnopharmacol 103:120-5. 2006.... - Four new lignans from the stems of Kadsura heteroclitaWei Wang
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, PR China
Planta Med 72:284-8. 2006..The structures of these compounds were characterized by extensive 1D and 2D NMR spectral data analysis... - Expression cloning of functional receptor used by SARS coronavirusPeigang Wang
Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China
Biochem Biophys Res Commun 315:439-44. 2004..When human ACE2 cDNA was transduced into NIH3T3 cells, the ACE2 expressing NIH3T3 cells could be infected with HIV/SARS pseudovirus. These data clearly demonstrated that ACE2 was the functional receptor for SARS-CoV... - Statistical optimization of sulfite pretreatment of corncob residues for high concentration ethanol productionKe Ke Cheng
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, PR China
Bioresour Technol 102:3014-9. 2011..In the subsequent simultaneous saccharification and fermentation (SSF) experiments using 15% glucan substrates pretreated under this kind of conditions, 60.8 g ethanol l(-1) with 72.2% theoretical yield was obtained... - Suspended nanoparticle crystal (S-NPC): A nanofluidics-based, electrical read-out biosensorYinhua Lei
Institute of Microelectronics, Peking University, Beijing, PR China
Lab Chip 10:2338-40. 2010..Being easy to get established, low-cost, and having large electrical read-out signal, the present S-NPC is believed to be a promising biosensing scheme in the micro total analysis system... - Drought tolerance through overexpression of monoubiquitin in transgenic tobaccoQifang Guo
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Shandong, PR China
J Plant Physiol 165:1745-55. 2008..Our results indicate that Ub may be involved in the response of plants to drought stress and that overexpression of monoubiquitin might be an effective strategy for enhancing drought tolerance... - Stretch-activated potassium channels in hypotonically induced blebs of atrial myocytesXuxia Liu
Department of Physiology, Capital Medical University, Beijing, PR China
J Membr Biol 226:17-25. 2008..The mechanosensitivity of SAKCs appears to be the result of interaction with membrane lipids and not of direct involvement of the cytoskeleton... - The impact of demographic and risk factor changes on coronary heart disease deaths in Beijing, 1999-2010Jun Cheng
Department of Epidemiology, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing Anzhen Hospital affiliated to the Capital Medical University, Beijing, PR China
BMC Public Health 9:30. 2009..Our study aimed to assess the potential impact of subsequent changes in risk factors and population ageing on CHD deaths in Beijing between 1999 and 2010... - Cord blood-derived cytokine-induced killer cells biotherapy combined with second-line chemotherapy in the treatment of advanced solid malignanciesQi Niu
Beijing GreatWall International Cancer Center, No 309 PLA Hospital, Beijing, PR China
Int Immunopharmacol 11:449-56. 2011....