Genomes and Genes
William C Skarnes
Affiliation: Wellcome Trust Genome Campus
- BayGenomics: a resource of insertional mutations in mouse embryonic stem cellsDoug Stryke
Department of Pharmaceutical Chemistry, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
Nucleic Acids Res 31:278-81. 2003..They can then obtain the mutant ES cell line for the purpose of generating knockout mice...
- A conditional knockout resource for the genome-wide study of mouse gene functionWilliam C Skarnes
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nature 474:337-42. 2011....
- Floxin, a resource for genetically engineering mouse ESCsVeena Singla
Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA
Nat Methods 7:50-2. 2010..Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs...
- Two ways to trap a gene in miceWilliam C Skarnes
Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
Proc Natl Acad Sci U S A 102:13001-2. 2005
- The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouseAlex S Nord
University of California San Francisco, 600 16th Street, San Francisco, CA 94143 2240, USA
Nucleic Acids Res 34:D642-8. 2006....
- Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell linesMatthew P A Davis
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom
PLoS ONE 7:e41762. 2012..Additionally, our experiments have revealed a novel candidate for Dgcr8-independent microRNA genesis and highlighted the challenges currently facing miRNA annotation...
- Agouti C57BL/6N embryonic stem cells for mouse genetic resourcesStephen J Pettitt
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK
Nat Methods 6:493-5. 2009..These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background...
- Bi-allelic gene targeting in mouse embryonic stem cellsPeri H Tate
Wellcome Trust Sanger Institute, Morgan Building, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
Methods 53:331-8. 2011..Inducible homozygous cells described here provide a precisely controlled experimental system to study gene function in a model cell...
- Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cellsAlex S Nord
Department of Medicine, MacDonald Medical Research Laboratories, University of California at Los Angeles, California, USA
PLoS ONE 2:e617. 2007..However, the rules governing its efficiency are not fully understood, and the effects of vector design on the likelihood of gene-trapping events have not been tested on a genome-wide scale...
- BioMart as an integration solution for the International Knockout Mouse ConsortiumDarren J Oakley
The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH
Database (Oxford) 2011:bar028. 2011..Here, we describe these datasets in more detail and illustrate the functionality of the portal using several examples...
- The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping dataGautier Koscielny
European Molecular Biology Laboratory, European Bioinformatics Institute EMBL EBI, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK, Medical Research Council Harwell Mammalian Genetics Unit and Mary Lyon Centre, Harwell, Oxfordshire OX11 0RD, UK and Mouse Informatics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nucleic Acids Res 42:D802-9. 2014..As phenotype data become available for every gene in the mouse, the IMPC web portal will become an invaluable tool for researchers studying the genetic contributions of genes to human diseases. ..
- Extensive genomic copy number variation in embryonic stem cellsQi Liang
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
Proc Natl Acad Sci U S A 105:17453-6. 2008..Human ES (hES) cells and derived somatic lineages may be similarly affected, challenging the concept of a stable somatic genome...
- MiR-25 regulates Wwp2 and Fbxw7 and promotes reprogramming of mouse fibroblast cells to iPSCsDong Lu
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
PLoS ONE 7:e40938. 2012..Recent studies also demonstrate that some miRNAs have important roles in reprogramming somatic cells to induced pluripotent stem cells (iPSCs)...
- Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibitionJeremy F Reiter
Developmental and Stem Cell Biology Program, and Diabetes Center, University of California, San Francisco, 94143 0525, USA
Genes Dev 20:22-7. 2006..Interestingly, characterization of Tectonic Shh and Tectonic Smo double mutants indicates that Tectonic plays an additional role in repressing Hh pathway activity...
- The mammalian gene function resource: the International Knockout Mouse ConsortiumAllan Bradley
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
Mamm Genome 23:580-6. 2012..The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research...
- Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genesJacqueline K White
Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
Cell 154:452-64. 2013..Phenotypic data and more than 900 mutants are openly available for further analysis. PAPERCLIP: ..
- JAM-A expression during embryonic developmentJames J Parris
Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
Dev Dyn 233:1517-24. 2005..Thus, JAM-A is prominently expressed in embryonic vasculature and the epithelial components of several organ systems and may have an important role in their development...
- Endoplasmic reticulum stress induction of the Grp78/BiP promoter: activating mechanisms mediated by YY1 and its interactive chromatin modifiersPeter Baumeister
Department of Biochemistry and Molecular Biology, USC Norris Comprehensive Cancer Center, Keck School of Medicine, 1441 Eastlake Ave, Room 5308, MC 9176, Los Angeles, CA 90089 9176, USA
Mol Cell Biol 25:4529-40. 2005..A model for the ER stress-mediated transcription factor binding and chromatin modifications at the Grp78 promoter leading to its activation is proposed...
- A public gene trap resource for mouse functional genomicsWilliam C Skarnes
Nat Genet 36:543-4. 2004