William C Skarnes

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. pmc BayGenomics: a resource of insertional mutations in mouse embryonic stem cells
    Doug Stryke
    Department of Pharmaceutical Chemistry, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Nucleic Acids Res 31:278-81. 2003
  2. pmc A conditional knockout resource for the genome-wide study of mouse gene function
    William C Skarnes
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 474:337-42. 2011
  3. pmc Floxin, a resource for genetically engineering mouse ESCs
    Veena Singla
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA
    Nat Methods 7:50-2. 2010
  4. pmc Two ways to trap a gene in mice
    William C Skarnes
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 102:13001-2. 2005
  5. pmc The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse
    Alex S Nord
    University of California San Francisco, 600 16th Street, San Francisco, CA 94143 2240, USA
    Nucleic Acids Res 34:D642-8. 2006
  6. pmc Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines
    Matthew P A Davis
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom
    PLoS ONE 7:e41762. 2012
  7. pmc Agouti C57BL/6N embryonic stem cells for mouse genetic resources
    Stephen J Pettitt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK
    Nat Methods 6:493-5. 2009
  8. pmc Bi-allelic gene targeting in mouse embryonic stem cells
    Peri H Tate
    Wellcome Trust Sanger Institute, Morgan Building, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Methods 53:331-8. 2011
  9. pmc BioMart as an integration solution for the International Knockout Mouse Consortium
    Darren J Oakley
    The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH
    Database (Oxford) 2011:bar028. 2011
  10. pmc Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells
    Alex S Nord
    Department of Medicine, MacDonald Medical Research Laboratories, University of California at Los Angeles, California, USA
    PLoS ONE 2:e617. 2007

Collaborators

Detail Information

Publications19

  1. pmc BayGenomics: a resource of insertional mutations in mouse embryonic stem cells
    Doug Stryke
    Department of Pharmaceutical Chemistry, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Nucleic Acids Res 31:278-81. 2003
    ..They can then obtain the mutant ES cell line for the purpose of generating knockout mice...
  2. pmc A conditional knockout resource for the genome-wide study of mouse gene function
    William C Skarnes
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 474:337-42. 2011
    ....
  3. pmc Floxin, a resource for genetically engineering mouse ESCs
    Veena Singla
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA
    Nat Methods 7:50-2. 2010
    ..Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs...
  4. pmc Two ways to trap a gene in mice
    William C Skarnes
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 102:13001-2. 2005
  5. pmc The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse
    Alex S Nord
    University of California San Francisco, 600 16th Street, San Francisco, CA 94143 2240, USA
    Nucleic Acids Res 34:D642-8. 2006
    ....
  6. pmc Large-scale identification of microRNA targets in murine Dgcr8-deficient embryonic stem cell lines
    Matthew P A Davis
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom
    PLoS ONE 7:e41762. 2012
    ..Additionally, our experiments have revealed a novel candidate for Dgcr8-independent microRNA genesis and highlighted the challenges currently facing miRNA annotation...
  7. pmc Agouti C57BL/6N embryonic stem cells for mouse genetic resources
    Stephen J Pettitt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK
    Nat Methods 6:493-5. 2009
    ..These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background...
  8. pmc Bi-allelic gene targeting in mouse embryonic stem cells
    Peri H Tate
    Wellcome Trust Sanger Institute, Morgan Building, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Methods 53:331-8. 2011
    ..Inducible homozygous cells described here provide a precisely controlled experimental system to study gene function in a model cell...
  9. pmc BioMart as an integration solution for the International Knockout Mouse Consortium
    Darren J Oakley
    The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH
    Database (Oxford) 2011:bar028. 2011
    ..Here, we describe these datasets in more detail and illustrate the functionality of the portal using several examples...
  10. pmc Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells
    Alex S Nord
    Department of Medicine, MacDonald Medical Research Laboratories, University of California at Los Angeles, California, USA
    PLoS ONE 2:e617. 2007
    ..However, the rules governing its efficiency are not fully understood, and the effects of vector design on the likelihood of gene-trapping events have not been tested on a genome-wide scale...
  11. pmc The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data
    Gautier Koscielny
    European Molecular Biology Laboratory, European Bioinformatics Institute EMBL EBI, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK, Medical Research Council Harwell Mammalian Genetics Unit and Mary Lyon Centre, Harwell, Oxfordshire OX11 0RD, UK and Mouse Informatics Group, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nucleic Acids Res 42:D802-9. 2014
    ..As phenotype data become available for every gene in the mouse, the IMPC web portal will become an invaluable tool for researchers studying the genetic contributions of genes to human diseases. ..
  12. pmc Extensive genomic copy number variation in embryonic stem cells
    Qi Liang
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:17453-6. 2008
    ..Human ES (hES) cells and derived somatic lineages may be similarly affected, challenging the concept of a stable somatic genome...
  13. pmc MiR-25 regulates Wwp2 and Fbxw7 and promotes reprogramming of mouse fibroblast cells to iPSCs
    Dong Lu
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS ONE 7:e40938. 2012
    ..Recent studies also demonstrate that some miRNAs have important roles in reprogramming somatic cells to induced pluripotent stem cells (iPSCs)...
  14. pmc Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition
    Jeremy F Reiter
    Developmental and Stem Cell Biology Program, and Diabetes Center, University of California, San Francisco, 94143 0525, USA
    Genes Dev 20:22-7. 2006
    ..Interestingly, characterization of Tectonic Shh and Tectonic Smo double mutants indicates that Tectonic plays an additional role in repressing Hh pathway activity...
  15. pmc The mammalian gene function resource: the International Knockout Mouse Consortium
    Allan Bradley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
    Mamm Genome 23:580-6. 2012
    ..The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research...
  16. pmc Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes
    Jacqueline K White
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Cell 154:452-64. 2013
    ..Phenotypic data and more than 900 mutants are openly available for further analysis. PAPERCLIP: ..
  17. ncbi request reprint JAM-A expression during embryonic development
    James J Parris
    Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
    Dev Dyn 233:1517-24. 2005
    ..Thus, JAM-A is prominently expressed in embryonic vasculature and the epithelial components of several organ systems and may have an important role in their development...
  18. pmc Endoplasmic reticulum stress induction of the Grp78/BiP promoter: activating mechanisms mediated by YY1 and its interactive chromatin modifiers
    Peter Baumeister
    Department of Biochemistry and Molecular Biology, USC Norris Comprehensive Cancer Center, Keck School of Medicine, 1441 Eastlake Ave, Room 5308, MC 9176, Los Angeles, CA 90089 9176, USA
    Mol Cell Biol 25:4529-40. 2005
    ..A model for the ER stress-mediated transcription factor binding and chromatin modifications at the Grp78 promoter leading to its activation is proposed...
  19. pmc A public gene trap resource for mouse functional genomics
    William C Skarnes
    Nat Genet 36:543-4. 2004