M A Quail

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. ncbi request reprint M13 cloning of mung bean nuclease digested PCR fragments as a means of gap closure within A/T-rich, genome sequencing projects
    M A Quail
    The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    DNA Seq 12:355-9. 2001
  2. doi request reprint SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing
    Michael A Quail
    Wellcome Trust Sanger Institute, Hinxton CB10 1SA, Cambs, UK
    BMC Genomics 15:110. 2014
  3. doi request reprint Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation
    Michael A Quail
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK
    Electrophoresis 33:3521-8. 2012
  4. pmc A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers
    Michael A Quail
    Wellcome Trust Sanger Institute, Hinxton, UK
    BMC Genomics 13:341. 2012
  5. pmc Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes
    Samuel O Oyola
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
    BMC Genomics 13:1. 2012
  6. pmc The genome of Rhizobium leguminosarum has recognizable core and accessory components
    J Peter W Young
    Department of Biology, University of York, York, UK
    Genome Biol 7:R34. 2006
  7. pmc The complete genome, comparative and functional analysis of Stenotrophomonas maltophilia reveals an organism heavily shielded by drug resistance determinants
    Lisa C Crossman
    Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK
    Genome Biol 9:R74. 2008
  8. pmc Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens
    Mark W Silby
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Centre for Adaptation Genetics and Drug Resistance, Boston, MA 02111, USA
    Genome Biol 10:R51. 2009
  9. pmc Independent evolution of the core and accessory gene sets in the genus Neisseria: insights gained from the genome of Neisseria lactamica isolate 020-06
    Julia S Bennett
    Department of Zoology, University of Oxford, UK
    BMC Genomics 11:652. 2010
  10. pmc Insights into the genome sequence of a free-living Kinetoplastid: Bodo saltans (Kinetoplastida: Euglenozoa)
    Andrew P Jackson
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    BMC Genomics 9:594. 2008

Detail Information

Publications34

  1. ncbi request reprint M13 cloning of mung bean nuclease digested PCR fragments as a means of gap closure within A/T-rich, genome sequencing projects
    M A Quail
    The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    DNA Seq 12:355-9. 2001
    ....
  2. doi request reprint SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing
    Michael A Quail
    Wellcome Trust Sanger Institute, Hinxton CB10 1SA, Cambs, UK
    BMC Genomics 15:110. 2014
    ..From the final sequencing data, one can verify that all the reads derive from the original sample(s) and not from contaminants or other samples...
  3. doi request reprint Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation
    Michael A Quail
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK
    Electrophoresis 33:3521-8. 2012
    ..We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries...
  4. pmc A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers
    Michael A Quail
    Wellcome Trust Sanger Institute, Hinxton, UK
    BMC Genomics 13:341. 2012
    ..3 to 67.7%. Together, these represent a comprehensive range of genome content. Here we report our analysis of that sequence data in terms of coverage distribution, bias, GC distribution, variant detection and accuracy...
  5. pmc Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes
    Samuel O Oyola
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
    BMC Genomics 13:1. 2012
    ..We have developed and optimized library-preparation procedures suitable for low quantity starting material and tolerant to extremely high AT content sequences...
  6. pmc The genome of Rhizobium leguminosarum has recognizable core and accessory components
    J Peter W Young
    Department of Biology, University of York, York, UK
    Genome Biol 7:R34. 2006
    ..We have sequenced the genome of R. leguminosarum biovar viciae strain 3841...
  7. pmc The complete genome, comparative and functional analysis of Stenotrophomonas maltophilia reveals an organism heavily shielded by drug resistance determinants
    Lisa C Crossman
    Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK
    Genome Biol 9:R74. 2008
    ..Whilst relatively distant phylogenetically, the closest sequenced relatives of S. maltophilia are the plant pathogenic xanthomonads...
  8. pmc Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens
    Mark W Silby
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Centre for Adaptation Genetics and Drug Resistance, Boston, MA 02111, USA
    Genome Biol 10:R51. 2009
    ..A functional genomic in vivo expression technology (IVET) screen provided insight into genes used by P. fluorescens in its natural environment and an improved understanding of the ecological significance of diversity within this species...
  9. pmc Independent evolution of the core and accessory gene sets in the genus Neisseria: insights gained from the genome of Neisseria lactamica isolate 020-06
    Julia S Bennett
    Department of Zoology, University of Oxford, UK
    BMC Genomics 11:652. 2010
    ..Genomic comparisons of these three bacteria will provide insights into the mechanisms and evolution of pathogenesis in this group of organisms, which are applicable to understanding these processes more generally...
  10. pmc Insights into the genome sequence of a free-living Kinetoplastid: Bodo saltans (Kinetoplastida: Euglenozoa)
    Andrew P Jackson
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    BMC Genomics 9:594. 2008
    ..Various coding regions were sequenced to provide a preliminary insight into the bodonid genome sequence, relative to trypanosomatid sequences...
  11. pmc Pseudogene accumulation in the evolutionary histories of Salmonella enterica serovars Paratyphi A and Typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    BMC Genomics 10:36. 2009
    ..The accumulation of pseudogenes is a key feature of these and other host-adapted pathogens, and overlapping pseudogene complements are evident in Paratyphi A and Typhi...
  12. pmc The genome sequence of the fish pathogen Aliivibrio salmonicida strain LFI1238 shows extensive evidence of gene decay
    Erik Hjerde
    Department of Molecular Biotechnology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, N 9037 Tromsø, Norway
    BMC Genomics 9:616. 2008
    ..The fish pathogen Aliivibrio salmonicida is the causative agent of cold-water vibriosis in marine aquaculture. The Gram-negative bacterium causes tissue degradation, hemolysis and sepsis in vivo...
  13. pmc Comparative genomics of the emerging human pathogen Photorhabdus asymbiotica with the insect pathogen Photorhabdus luminescens
    Paul Wilkinson
    School of Biosciences, University of Exeter in Cornwall, Penryn TR10 9EZ, UK
    BMC Genomics 10:302. 2009
    ..Here we compare the complete genome of Pa ATCC43949 with that of the previously sequenced insect pathogen P. luminescens strain TT01 which was isolated from its entomopathogenic nematode vector collected from soil in Trinidad and Tobago...
  14. pmc Evidence for a lineage of virulent bacteriophages that target Campylobacter
    Andrew R Timms
    Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK
    BMC Genomics 11:214. 2010
    ..Campylobacter, a food-borne pathogen, is one of the organisms for which the use of bacteriophage is being considered to reduce human exposure to this organism...
  15. pmc Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain
    Helena M B Seth-Smith
    Molecular Microbiology Group, University Medical School, Southampton General Hospital, Southampton, SO16 6YD, UK
    BMC Genomics 10:239. 2009
    ..This is essential knowledge if the plasmid is to be continued to be relied on as a key diagnostic marker, and for an understanding of the evolution of Chlamydia trachomatis...
  16. pmc The Mycoplasma conjunctivae genome sequencing, annotation and analysis
    Sandra P Calderon-Copete
    Swiss Institute of Bioinformatics, Génopode UNIL, 1015 Lausanne, Switzerland
    BMC Bioinformatics 10:S7. 2009
    ..This is a major cause of death in the protected species in the Alps (e.g., Capra ibex, Rupicapra rupicapra)...
  17. ncbi request reprint The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum
    S Bowman
    Pathogen Sequencing Unit, Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK
    Nature 400:532-8. 1999
    ..The predicted protein-coding genes can be divided into three main classes: 52.6% are not spliced, 45.1% have a large exon with short additional 5' or 3' exons, and 2.3% have a multiple exon structure more typical of higher eukaryotes...
  18. pmc The genome of the simian and human malaria parasite Plasmodium knowlesi
    A Pain
    Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    Nature 455:799-803. 2008
    ..One of these families, the KIRs, contains sequences that collectively match over one-half of the host CD99 extracellular domain, which may represent an unusual form of molecular mimicry...
  19. ncbi request reprint Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491
    J Parkhill
    The Sanger Centre, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nature 404:502-6. 2000
    ..Many of these repeats appear to be involved in genome fluidity and antigenic variation in this important human pathogen...
  20. pmc Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors
    K S Bell
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 101:11105-10. 2004
    ....
  21. ncbi request reprint Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
    S T Cole
    Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK
    Nature 393:537-44. 1998
    ....
  22. ncbi request reprint SCP1, a 356,023 bp linear plasmid adapted to the ecology and developmental biology of its host, Streptomyces coelicolor A3(2)
    S D Bentley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Mol Microbiol 51:1615-28. 2004
    ..This may be related to atypical features of its end sequences...
  23. ncbi request reprint Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)
    S D Bentley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 417:141-7. 2002
    ..The genome sequence will greatly increase our understanding of microbial life in the soil as well as aiding the generation of new drug candidates by genetic engineering...
  24. ncbi request reprint The genome sequence of Schizosaccharomyces pombe
    V Wood
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nature 415:871-80. 2002
    ....
  25. pmc The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129
    A M Cerdeño-Tárraga
    The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nucleic Acids Res 31:6516-23. 2003
    ..The genome itself shows an unusually extreme large-scale compositional bias, being noticeably higher in G+C near the origin than at the terminus...
  26. ncbi request reprint Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13
    N Hall
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 419:527-31. 2002
    ..We identify a highly conserved sequence element found in the intergenic region of internal var genes that is not associated with their telomeric counterparts...
  27. pmc The genome of the social amoeba Dictyostelium discoideum
    L Eichinger
    Center for Biochemistry and Center for Molecular Medicine Cologne, University of Cologne, Joseph Stelzmann Str 52, 50931 Cologne, Germany
    Nature 435:43-57. 2005
    ....
  28. ncbi request reprint A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system
    S C Andrews
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Microbiology 143:3633-47. 1997
    ..It is likely that HyfR acts as a formate-dependent regulator of the hyf operon and that FocB provides the Hyf complex with external formate as substrate...
  29. ncbi request reprint How the presence of three iron binding sites affects the iron storage function of the ferritin (EcFtnA) of Escherichia coli
    A Treffry
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 432:213-8. 1998
    ..However a compensatory physiological advantage is conferred because this iron is more readily available to meet the cell's needs...
  30. ncbi request reprint Spectroscopic and voltammetric characterisation of the bacterioferritin-associated ferredoxin of Escherichia coli
    M A Quail
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, United Kingdom
    Biochem Biophys Res Commun 229:635-42. 1996
    ..Bfd may thus be a 2Fe ferredoxin participating either in release/delivery of iron from/to bacterioferritin (or other iron complexes), or in iron-dependent regulation of bfr expression...
  31. ncbi request reprint A mutation causing constitutive synthesis of the pyruvate dehydrogenase complex in Escherichia coli is located within the pdhR gene
    D J Haydon
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, UK
    FEBS Lett 336:43-7. 1993
    ..The results support the view that synthesis of the PDH complex is regulated from the pdhR promoter of a pdhR-aceEF-lpd operon...
  32. ncbi request reprint The pdhR-aceEF-lpd operon of Escherichia coli expresses the pyruvate dehydrogenase complex
    M A Quail
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 12:95-104. 1994
    ..The partially purified PdhR protein was also found to specifically retard and protect DNA fragments containing the pdh promoter region. The pdh promoter was not strongly controlled by ArcA, FNR or CRP...
  33. ncbi request reprint Identification of a fatty acyl responsive regulator (FarR) in Escherichia coli
    M A Quail
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 356:183-7. 1994
    ..It is suggested that FarR autoregulates farR expression and may modulate citric acid cycle expression in response to long-chain fatty acids...
  34. ncbi request reprint Purification, characterization and mode of action of PdhR, the transcriptional repressor of the pdhR-aceEF-lpd operon of Escherichia coli
    M A Quail
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 15:519-29. 1995
    ..It is concluded that PdhR represses transcription by binding to an operator site centred at +19 such that effective binding of RNA polymerase is prevented...