Research Topics
| Kathryn E HoltSummaryAffiliation: Wellcome Trust Genome Campus Country: UK Publications
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Detail Information
Publications
High-throughput sequencing provides insights into genome variation and evolution in Salmonella TyphiKathryn E Holt
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Nat Genet 40:987-93. 2008..The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers...- Detecting SNPs and estimating allele frequencies in clonal bacterial populations by sequencing pooled DNAKathryn E Holt
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Bioinformatics 25:2074-5. 2009..AVAILABILITY: The method was implemented in Perl and relies on the opensource software Maq for read mapping and SNP calling. The Perl script is freely available from ftp://ftp.sanger.ac.uk/pub/pathogens/pools/... - The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimensTran Vu Thieu Nga
Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
BMC Infect Dis 10:125. 2010..Invasive Salmonella serovars, which cause enteric fever, can be commonly cultured from the blood of infected patients. Yet, the isolation of invasive Salmonella serovars from blood is protracted and potentially insensitive... - Pseudogene accumulation in the evolutionary histories of Salmonella enterica serovars Paratyphi A and TyphiKathryn E Holt
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
BMC Genomics 10:36. 2009..The accumulation of pseudogenes is a key feature of these and other host-adapted pathogens, and overlapping pseudogene complements are evident in Paratyphi A and Typhi... - Multidrug-resistant Salmonella enterica serovar paratyphi A harbors IncHI1 plasmids similar to those found in serovar typhiKathryn E Holt
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
J Bacteriol 189:4257-64. 2007..Our data show that these serovar Typhi and Paratyphi A IncHI1 plasmids share highly conserved core DNA and have acquired similar mobile elements encoding antibiotic resistance genes in past decades... - Evolutionary dynamics of Clostridium difficile over short and long time scalesMiao He
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
Proc Natl Acad Sci U S A 107:7527-32. 2010..1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages... - Emergence of a globally dominant IncHI1 plasmid type associated with multiple drug resistant typhoidKathryn E Holt
Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
PLoS Negl Trop Dis 5:e1245. 2011..Typhi host. The PST6 plasmid conferred the ability to grow in high salt medium (4.7% NaCl), which we demonstrate is due to the presence in PST6 of the Tn6062 transposon encoding BetU... - High-throughput bacterial SNP typing identifies distinct clusters of Salmonella Typhi causing typhoid in Nepalese childrenKathryn E Holt
Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
BMC Infect Dis 10:144. 2010..Kathmandu, the capital of Nepal, is an area of high incidence and the pediatric population appears to be at high risk of exposure and infection... - Variation in Salmonella enterica serovar typhi IncHI1 plasmids during the global spread of resistant typhoid feverMinh Duy Phan
The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
Antimicrob Agents Chemother 53:716-27. 2009..We propose that antibiotic selection acts to maintain resistance genes on the plasmid, but there is also competition between plasmids encoding the same resistance phenotype... - A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivoAngela M Jansen
The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
PLoS Pathog 7:e1002131. 2011..The presence of the Vi capsule also correlated with induction of the anti-inflammatory cytokine IL-10 in vivo, a factor that impacted on chemotaxis and the activation of immune cells in vitro... - Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of VietnamKathryn E Holt
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
PLoS Negl Trop Dis 5:e929. 2011..Typhi isolated from 267 typhoid fever patients in the Mekong delta region participating in a randomized trial conducted between 2004 and 2005... - Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotypeRobert A Kingsley
The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Cambridge, United Kingdom
Genome Res 19:2279-87. 2009..Typhi are either pseudogenes or are absent. Genome analysis of other epidemic ST313 isolates from Malawi and Kenya provided evidence for microevolution and clonal replacement in the field...