Kathryn E Holt

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. pmc High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi
    Kathryn E Holt
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Genet 40:987-93. 2008
  2. pmc Detecting SNPs and estimating allele frequencies in clonal bacterial populations by sequencing pooled DNA
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Bioinformatics 25:2074-5. 2009
  3. pmc The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens
    Tran Vu Thieu Nga
    Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
    BMC Infect Dis 10:125. 2010
  4. pmc Pseudogene accumulation in the evolutionary histories of Salmonella enterica serovars Paratyphi A and Typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    BMC Genomics 10:36. 2009
  5. pmc Multidrug-resistant Salmonella enterica serovar paratyphi A harbors IncHI1 plasmids similar to those found in serovar typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    J Bacteriol 189:4257-64. 2007
  6. pmc Evolutionary dynamics of Clostridium difficile over short and long time scales
    Miao He
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 107:7527-32. 2010
  7. pmc Emergence of a globally dominant IncHI1 plasmid type associated with multiple drug resistant typhoid
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    PLoS Negl Trop Dis 5:e1245. 2011
  8. pmc High-throughput bacterial SNP typing identifies distinct clusters of Salmonella Typhi causing typhoid in Nepalese children
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    BMC Infect Dis 10:144. 2010
  9. pmc Variation in Salmonella enterica serovar typhi IncHI1 plasmids during the global spread of resistant typhoid fever
    Minh Duy Phan
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    Antimicrob Agents Chemother 53:716-27. 2009
  10. pmc A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo
    Angela M Jansen
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002131. 2011

Collaborators

Detail Information

Publications14

  1. pmc High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi
    Kathryn E Holt
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Genet 40:987-93. 2008
    ..The observed patterns of genetic isolation and drift are consistent with the proposed key role of asymptomatic carriers of Typhi as the main reservoir of this pathogen, highlighting the need for identification and treatment of carriers...
  2. pmc Detecting SNPs and estimating allele frequencies in clonal bacterial populations by sequencing pooled DNA
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Bioinformatics 25:2074-5. 2009
    ....
  3. pmc The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens
    Tran Vu Thieu Nga
    Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam
    BMC Infect Dis 10:125. 2010
    ..Invasive Salmonella serovars, which cause enteric fever, can be commonly cultured from the blood of infected patients. Yet, the isolation of invasive Salmonella serovars from blood is protracted and potentially insensitive...
  4. pmc Pseudogene accumulation in the evolutionary histories of Salmonella enterica serovars Paratyphi A and Typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    BMC Genomics 10:36. 2009
    ..The accumulation of pseudogenes is a key feature of these and other host-adapted pathogens, and overlapping pseudogene complements are evident in Paratyphi A and Typhi...
  5. pmc Multidrug-resistant Salmonella enterica serovar paratyphi A harbors IncHI1 plasmids similar to those found in serovar typhi
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    J Bacteriol 189:4257-64. 2007
    ..Our data show that these serovar Typhi and Paratyphi A IncHI1 plasmids share highly conserved core DNA and have acquired similar mobile elements encoding antibiotic resistance genes in past decades...
  6. pmc Evolutionary dynamics of Clostridium difficile over short and long time scales
    Miao He
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 107:7527-32. 2010
    ..1-85 million years. By contrast, the disease-causing isolates have arisen from multiple lineages, suggesting that virulence evolved independently in the highly epidemic lineages...
  7. pmc Emergence of a globally dominant IncHI1 plasmid type associated with multiple drug resistant typhoid
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    PLoS Negl Trop Dis 5:e1245. 2011
    ..Typhi host. The PST6 plasmid conferred the ability to grow in high salt medium (4.7% NaCl), which we demonstrate is due to the presence in PST6 of the Tn6062 transposon encoding BetU...
  8. pmc High-throughput bacterial SNP typing identifies distinct clusters of Salmonella Typhi causing typhoid in Nepalese children
    Kathryn E Holt
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    BMC Infect Dis 10:144. 2010
    ..Kathmandu, the capital of Nepal, is an area of high incidence and the pediatric population appears to be at high risk of exposure and infection...
  9. pmc Variation in Salmonella enterica serovar typhi IncHI1 plasmids during the global spread of resistant typhoid fever
    Minh Duy Phan
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    Antimicrob Agents Chemother 53:716-27. 2009
    ..We propose that antibiotic selection acts to maintain resistance genes on the plasmid, but there is also competition between plasmids encoding the same resistance phenotype...
  10. pmc A Salmonella Typhimurium-Typhi genomic chimera: a model to study Vi polysaccharide capsule function in vivo
    Angela M Jansen
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Pathog 7:e1002131. 2011
    ..The presence of the Vi capsule also correlated with induction of the anti-inflammatory cytokine IL-10 in vivo, a factor that impacted on chemotaxis and the activation of immune cells in vitro...
  11. pmc Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of Vietnam
    Kathryn E Holt
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    PLoS Negl Trop Dis 5:e929. 2011
    ..Typhi isolated from 267 typhoid fever patients in the Mekong delta region participating in a randomized trial conducted between 2004 and 2005...
  12. pmc Genome and transcriptome adaptation accompanying emergence of the definitive type 2 host-restricted Salmonella enterica serovar Typhimurium pathovar
    Robert A Kingsley
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    MBio 4:e00565-13. 2013
    ..Typhimurium isolates alongside phenotypic and transcriptome analyses, to identify genetic signatures linked to host adaptation within the DT2 lineage...
  13. pmc Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype
    Robert A Kingsley
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2279-87. 2009
    ..Typhi are either pseudogenes or are absent. Genome analysis of other epidemic ST313 isolates from Malawi and Kenya provided evidence for microevolution and clonal replacement in the field...
  14. pmc Characterization of the yehUT Two-Component Regulatory System of Salmonella enterica Serovar Typhi and Typhimurium
    Vanessa K Wong
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom University of Cambridge Department of Pathology, Addenbrooke s Hospital, Cambridge, United Kingdom
    PLoS ONE 8:e84567. 2013
    ..Typhi and S. Typhimurium) or induce colitis in a murine model (S. Typhimurium only). Growth, metabolic and antimicrobial susceptibility tests identified no obvious influences of yehUT on these phenotypes. ..