Andrew G Fraser

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. ncbi request reprint Uncover genetic interactions in Caenorhabditis elegans by RNA interference
    Angelo Fortunato
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Cambridge, Hinxton, UK
    Biosci Rep 25:299-307. 2005
  2. pmc Systems biology: where it's at in 2005
    Ben Lehner
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 6:338. 2005
  3. ncbi request reprint Development through the eyes of functional genomics
    Andrew G Fraser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Curr Opin Genet Dev 14:336-42. 2004
  4. ncbi request reprint A probabilistic view of gene function
    Andrew G Fraser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Genet 36:559-64. 2004
  5. ncbi request reprint Towards full employment: using RNAi to find roles for the redundant
    Andrew Fraser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Oncogene 23:8346-52. 2004
  6. ncbi request reprint Worms in L.A
    Andrew Fraser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Genet 35:3-5. 2003
  7. pmc Loss of LIN-35, the Caenorhabditis elegans ortholog of the tumor suppressor p105Rb, results in enhanced RNA interference
    Ben Lehner
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 7:R4. 2006
  8. doi request reprint Evolutionary plasticity of genetic interaction networks
    Julia Tischler
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nat Genet 40:390-1. 2008
  9. pmc Control of feeding behavior in C. elegans by human G protein-coupled receptors permits screening for agonist-expressing bacteria
    Michelle S Teng
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1HH, United Kingdom
    Proc Natl Acad Sci U S A 105:14826-31. 2008
  10. ncbi request reprint Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways
    Ben Lehner
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nat Genet 38:896-903. 2006

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Uncover genetic interactions in Caenorhabditis elegans by RNA interference
    Angelo Fortunato
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, CB10 1SA, Cambridge, Hinxton, UK
    Biosci Rep 25:299-307. 2005
    ..In this review, we illustrate how RNAi has been used to analyse gene function in C. elegans as well as pointing to some future directions for using RNAi to examine genetic interactions in a systematic manner...
  2. pmc Systems biology: where it's at in 2005
    Ben Lehner
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 6:338. 2005
    ..A report on the joint Keystone Symposia on Systems and Biology and Proteomics and Bioinformatics, Keystone, USA, 8-13 April 2005...
  3. ncbi request reprint Development through the eyes of functional genomics
    Andrew G Fraser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Curr Opin Genet Dev 14:336-42. 2004
    ....
  4. ncbi request reprint A probabilistic view of gene function
    Andrew G Fraser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nat Genet 36:559-64. 2004
    ..Even this comprehensive view fails to capture key aspects of gene function, not least their dynamics in time and space, showing that there are limitations to the model that must ultimately be addressed...
  5. ncbi request reprint Towards full employment: using RNAi to find roles for the redundant
    Andrew Fraser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Oncogene 23:8346-52. 2004
    ..elegans. In particular, I discuss the use of genome-wide RNAi screens in C. elegans to identify synthetic lethal interactions and compare this with synthetic lethal interaction analysis in Saccharomyces cerevisiae...
  6. ncbi request reprint Worms in L.A
    Andrew Fraser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Genet 35:3-5. 2003
  7. pmc Loss of LIN-35, the Caenorhabditis elegans ortholog of the tumor suppressor p105Rb, results in enhanced RNA interference
    Ben Lehner
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 7:R4. 2006
    ..The effectiveness of RNAi varies from gene to gene, however, and neuronally expressed genes are largely refractive to RNAi in wild-type worms...
  8. doi request reprint Evolutionary plasticity of genetic interaction networks
    Julia Tischler
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nat Genet 40:390-1. 2008
    ..Genetic interactions are therefore unlikely to represent simple redundancy between genes or pathways, and genetic interactions from yeast do not directly predict genetic interactions in higher eukaryotes, including humans...
  9. pmc Control of feeding behavior in C. elegans by human G protein-coupled receptors permits screening for agonist-expressing bacteria
    Michelle S Teng
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1HH, United Kingdom
    Proc Natl Acad Sci U S A 105:14826-31. 2008
    ..This approach serves not only for structure/function studies as demonstrated, but may be used to facilitate the discovery of agonists within bacterial libraries...
  10. ncbi request reprint Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways
    Ben Lehner
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nat Genet 38:896-903. 2006
    ..We propose that these genes function as general buffers of genetic variation and that these hub genes may act as modifier genes in multiple, mechanistically unrelated genetic diseases in humans...
  11. pmc Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution
    Julia Tischler
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK
    Genome Biol 7:R69. 2006
    ..Developing tools for the systematic mapping of genetic interactions is thus a key step in exploring the relationship between genotype and phenotype...
  12. ncbi request reprint Protein domains enriched in mammalian tissue-specific or widely expressed genes
    Ben Lehner
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Trends Genet 20:468-72. 2004
    ..These findings identify families of proteins that are probably used in the development or terminal differentiation of many different tissue types...
  13. ncbi request reprint RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions
    Ben Lehner
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK
    Nat Protoc 1:1617-20. 2006
    ..We also describe how the protocol can be adapted to target two genes simultaneously by combinatorial RNAi...
  14. pmc Expression of mammalian GPCRs in C. elegans generates novel behavioural responses to human ligands
    Michelle S Teng
    Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK
    BMC Biol 4:22. 2006
    ..We sought to test this by functionally expressing two medically important mammalian GPCRs, somatostatin receptor 2 (Sstr2) and chemokine receptor 5 (CCR5) in the gustatory neurons of C. elegans...
  15. pmc Nuclear receptor binding protein 1 regulates intestinal progenitor cell homeostasis and tumour formation
    Catherine H Wilson
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, UK
    EMBO J 31:2486-97. 2012
    ..Thus NRBP1 is a conserved regulator of cell fate, that plays an important role in tumour suppression...
  16. pmc A first-draft human protein-interaction map
    Ben Lehner
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 5:R63. 2004
    ..Because many physical interactions are conserved between species, it should be possible to infer information about human protein interactions (and hence protein function) using model organism protein-interaction datasets...
  17. pmc Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans
    Gino Poulin
    Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
    EMBO J 24:2613-23. 2005
    ..As most of the genes identified in this screen are conserved in humans, we suggest that similar interactions may be relevant in mammals for control of Ras and Notch signalling, crosstalk between these pathways, and cell proliferation...
  18. ncbi request reprint Technique review: how to use RNA interference
    Ben Lehner
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK
    Brief Funct Genomic Proteomic 3:68-83. 2004
    ..Guidelines for designing effective small interfering RNAs and appropriate controls for mammalian RNAi experiments will also be discussed...
  19. pmc Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis
    Ian Sudbery
    Work performed at Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    BMC Genomics 11:175. 2010
    ..We have conducted a careful examination of off-target effects during an siRNA screen for novel regulators of the TRAIL apoptosis induction pathway(s)...
  20. ncbi request reprint Treating genetic disease through RNA interference
    Catriona Crombie
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Lancet 365:1288-90. 2005
  21. pmc The not-so-humble worm
    Catriona Crombie
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Genome Biol 5:301. 2003
  22. doi request reprint A single gene network accurately predicts phenotypic effects of gene perturbation in Caenorhabditis elegans
    Insuk Lee
    Center for Systems and Synthetic Biology, Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas, 2500 Speedway, MBB 3 210, Austin, Texas 78712, USA
    Nat Genet 40:181-8. 2008
    ..We conclude that an analogous network for human genes might be similarly predictive and thus facilitate identification of disease genes and rational therapeutic targets...
  23. ncbi request reprint A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity
    Siu Sylvia Lee
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nat Genet 33:40-8. 2003
    ..These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity...
  24. ncbi request reprint Rates of behavior and aging specified by mitochondrial function during development
    Andrew Dillin
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 0448, USA
    Science 298:2398-401. 2002
    ..Thus, the developing animal appears to contain a regulatory system that monitors mitochondrial activity early in life and, in response, establishes rates of respiration, behavior, and aging that persist during adulthood...
  25. ncbi request reprint Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
    Ravi S Kamath
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Nature 421:231-7. 2003
    ..Our resulting data set and reusable RNAi library of 16,757 bacterial clones will facilitate systematic analyses of the connections among gene sequence, chromosomal location and gene function in C. elegans...
  26. ncbi request reprint Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes
    Kaveh Ashrafi
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 421:268-72. 2003
    ....
  27. ncbi request reprint A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans
    Nadine L Vastenhouw
    Hubrecht Laboratory and Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Curr Biol 13:1311-6. 2003
    ..Since most of the newly identified genes have clear homologs in other species, and since transposons are found from protozoa to human, it seems likely that they also protect other genomes against transposon activity in the germline...
  28. pmc Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions
    Femke Simmer
    Hubrecht Laboratory, Centre for Biomedical Genetics, Utrecht, The Netherlands
    PLoS Biol 1:E12. 2003
    ..elegans genome. The resulting dataset will be valuable in conjunction with other functional genomics approaches, as well as in other model organisms...
  29. pmc Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi
    Joris Pothof
    Hubrecht Laboratory, Center for Biomedical Genetics, 3584 CT, Utrecht, The Netherlands
    Genes Dev 17:443-8. 2003
    ..Because known mutator genes are causally involved in many hereditary and sporadic human cancers, it is likely that some of these new mutators are equally relevant in cancer etiology...
  30. ncbi request reprint Minimizing the risk of reporting false positives in large-scale RNAi screens
    Christophe J Echeverri
    Cenix BioScience GmbH, Tatzberg 47, Dresden, 10307, Germany
    Nat Methods 3:777-9. 2006
    ....
  31. ncbi request reprint 5,000 RNAi experiments on a chip
    Ben Lehner
    Nat Methods 1:103-4. 2004
  32. pmc Genetic analysis of tissue aging in Caenorhabditis elegans: a role for heat-shock factor and bacterial proliferation
    Delia Garigan
    Department of Biochemistry and Biophysics and Program in Neuroscience, University of California San Francisco, San Francisco, CA 94143 0448, USA
    Genetics 161:1101-12. 2002
    ..Finally, we have identified a cause of death of C. elegans: namely, proliferating bacteria. This suggests that increased susceptibility to bacterial infections contributes to mortality in these animals, just as it does in humans...