Allan Bradley

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. pmc The mammalian gene function resource: the International Knockout Mouse Consortium
    Allan Bradley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
    Mamm Genome 23:580-6. 2012
  2. pmc A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells
    Wei Wang
    Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, PR China
    Genome Biol 8:R48. 2007
  3. pmc A mouse chromosome 4 balancer ENU-mutagenesis screen isolates eleven lethal lines
    Melissa K Boles
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    BMC Genet 10:12. 2009
  4. pmc Mouse chromosome engineering for modeling human disease
    Louise van der Weyden
    Mouse Genomics Lab, Wellcome Trust Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Annu Rev Genomics Hum Genet 7:247-76. 2006
  5. pmc A resource of vectors and ES cells for targeted deletion of microRNAs in mice
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Biotechnol 29:840-5. 2011
  6. pmc Mosaic complementation demonstrates a regulatory role for myosin VIIa in actin dynamics of stereocilia
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Mol Cell Biol 28:1702-12. 2008
  7. pmc An expanded Oct4 interaction network: implications for stem cell biology, development, and disease
    Mercedes Pardo
    Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK
    Cell Stem Cell 6:382-95. 2010
  8. pmc Mutant nucleophosmin and cooperating pathways drive leukemia initiation and progression in mice
    George S Vassiliou
    Mouse Genomics Team, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Genet 43:470-5. 2011
  9. pmc A conditional knockout resource for the genome-wide study of mouse gene function
    William C Skarnes
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 474:337-42. 2011
  10. pmc Molecular mechanisms governing Pcdh-gamma gene expression: evidence for a multiple promoter and cis-alternative splicing model
    Xiaozhong Wang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 16:1890-905. 2002

Research Grants

Collaborators

Detail Information

Publications42

  1. pmc The mammalian gene function resource: the International Knockout Mouse Consortium
    Allan Bradley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
    Mamm Genome 23:580-6. 2012
    ..The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research...
  2. pmc A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells
    Wei Wang
    Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, PR China
    Genome Biol 8:R48. 2007
    ..In order to identify the host factors involved in retroviral infection, we designed and implemented a scheme for identifying ES cells that are resistant to retroviral infection and subsequent cloning of the mutated gene...
  3. pmc A mouse chromosome 4 balancer ENU-mutagenesis screen isolates eleven lethal lines
    Melissa K Boles
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    BMC Genet 10:12. 2009
    ..To functionally annotate the distal region of mouse chromosome 4, we performed an ENU-mutagenesis screen using a balancer chromosome targeted to this region of the genome...
  4. pmc Mouse chromosome engineering for modeling human disease
    Louise van der Weyden
    Mouse Genomics Lab, Wellcome Trust Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Annu Rev Genomics Hum Genet 7:247-76. 2006
    ..The resulting mouse models are leading to a better understanding of the molecular and cellular basis of dosage alterations in human disease phenotypes, in turn opening new diagnostic and therapeutic opportunities...
  5. pmc A resource of vectors and ES cells for targeted deletion of microRNAs in mice
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Biotechnol 29:840-5. 2011
    ..This miRNA knockout (mirKO) resource can be searched electronically and is available from ES cell repositories for distribution to the scientific community...
  6. pmc Mosaic complementation demonstrates a regulatory role for myosin VIIa in actin dynamics of stereocilia
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Mol Cell Biol 28:1702-12. 2008
    ..Our results strongly suggest that myosin VIIa regulates the establishment of a setpoint for stereocilium heights, and this novel role may influence their normal staircase-like arrangement within a bundle...
  7. pmc An expanded Oct4 interaction network: implications for stem cell biology, development, and disease
    Mercedes Pardo
    Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK
    Cell Stem Cell 6:382-95. 2010
    ..The Oct4 interactome provides a resource for dissecting mechanisms of Oct4 function, enlightening the basis of pluripotency and development, and identifying potential additional reprogramming factors...
  8. pmc Mutant nucleophosmin and cooperating pathways drive leukemia initiation and progression in mice
    George S Vassiliou
    Mouse Genomics Team, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Genet 43:470-5. 2011
    ..We also identified recurrent integrations in known and newly discovered leukemia genes including Nf1, Bach2, Dleu2 and Nup98. Our results provide new pathogenetic insights and identify possible therapeutic targets in NPM1c+ AML...
  9. pmc A conditional knockout resource for the genome-wide study of mouse gene function
    William C Skarnes
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 474:337-42. 2011
    ....
  10. pmc Molecular mechanisms governing Pcdh-gamma gene expression: evidence for a multiple promoter and cis-alternative splicing model
    Xiaozhong Wang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 16:1890-905. 2002
    ....
  11. pmc Induced mitotic recombination of p53 in vivo
    Wei Wang
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 104:4501-5. 2007
    ..Our results suggest that inducible mitotic recombination can be used for clonal analysis of mutants in the mouse...
  12. pmc Bigenic Cre/loxP, puDeltatk conditional genetic ablation
    You Tzung Chen
    Program in Developmental Biology, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Nucleic Acids Res 32:e161. 2004
    ..5 dpc embryos. Crosses between the puDeltatk selector transgenic line and existing cre lines will facilitate numerous temporally regulated tissue-specific ablation experiments...
  13. pmc Inducible gene trapping with drug-selectable markers and Cre/loxP to identify developmentally regulated genes
    You Tzung Chen
    Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, USA
    Mol Cell Biol 24:9930-41. 2004
    ..Furthermore, we demonstrate that the cre reporter is extremely sensitive and can be used to explore chromosomal regions that are not detectable with neo as a selection cassette...
  14. ncbi request reprint Mutagenic insertion and chromosome engineering resource (MICER)
    David J Adams
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs, CB10 1SA, UK
    Nat Genet 36:867-71. 2004
    ..These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome...
  15. ncbi request reprint Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells
    Ge Guo
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Nature 429:891-5. 2004
    ..The combination of insertional mutagenesis in Blm-deficient ES cells establishes a new approach for phenotype-based recessive genetic screens in ES cells...
  16. ncbi request reprint A targeted X-linked CMV-Cre line
    Hong Su
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Genesis 32:187-8. 2002
  17. ncbi request reprint Two new mouse chromosome 11 balancers
    Jan Klysik
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Genomics 83:303-10. 2004
    ..With the addition of these balancers to the previously reported Trp53-Wnt3 balancer, most of mouse chromosome 11 is now available in balancer stocks...
  18. ncbi request reprint Induced mitotic recombination: a switch in time
    David J Adams
    Nat Genet 30:6-7. 2002
  19. ncbi request reprint Visual genotyping of a coat color tagged p53 mutant mouse line
    Binhai Zheng
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Cancer Biol Ther 1:433-5. 2002
    ..Thus, tagging targeted mutations with such coat color markers provides a generally applicable genotyping method for embryonic stem cell-derived mice...
  20. pmc Modeling del(17)(p11.2p11.2) and dup(17)(p11.2p11.2) contiguous gene syndromes by chromosome engineering in mice: phenotypic consequences of gene dosage imbalance
    Katherina Walz
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Cell Biol 23:3646-55. 2003
    ..Our murine models represent a powerful tool to analyze the consequences of gene dosage imbalance in this genomic interval and to investigate the molecular genetic bases of both SMS and dup(17)(p11.2p11.2)...
  21. ncbi request reprint Transgenics at breaking-point
    Haydn Prosser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Cancer Cell 3:411-3. 2003
    ..2003) have generated mice that recapitulate both the mechanism (sporadic somatic translocation) and the consequences (expression of two translocation fusion genes) leading to an accurate leukemia model...
  22. pmc Urocortin-deficient mice display normal stress-induced anxiety behavior and autonomic control but an impaired acoustic startle response
    Xiaozhong Wang
    Department of Molecular and Human Genetics, Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:6605-10. 2002
    ..Ucn may modulate the acoustic startle response through the Ucn-expressing neuron projections from the region of the Edinger-Westphal nucleus...
  23. pmc Olfactory bulb hypoplasia in Prokr2 null mice stems from defective neuronal progenitor migration and differentiation
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Eur J Neurosci 26:3339-44. 2007
    ..Together, these findings suggest an important role for Prokr2 in OB neurogenesis...
  24. pmc Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster
    Ulrike C Lange
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Mol Cell Biol 28:4688-96. 2008
    ..Hence, contrary to previous reports, Ifitm genes are not essential for PGC migration. The Ifitm family is a striking example of a conserved gene cluster which appears to be functionally redundant during development...
  25. pmc Increased insulin action in SKIP heterozygous knockout mice
    Takeshi Ijuin
    Division of Lipid Biochemistry, Kobe University Graduate School of Medicine, Kobe 650 0017, Hyogo, Japan
    Mol Cell Biol 28:5184-95. 2008
    ..These results imply that SKIP regulates insulin signaling in skeletal muscle. Thus, SKIP may be a promising pharmacologic target for the treatment of insulin resistance and diabetes...
  26. pmc Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy
    Fung Chan
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:9109-14. 2004
    ....
  27. ncbi request reprint Conditional inactivation of p63 by Cre-mediated excision
    Alea A Mills
    Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Genesis 32:138-41. 2002
  28. pmc Mice deficient for the wild-type p53-induced phosphatase gene (Wip1) exhibit defects in reproductive organs, immune function, and cell cycle control
    Jene Choi
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:1094-105. 2002
    ..The data are consistent with an important role for Wip1 in spermatogenesis, lymphoid cell function, and cell cycle regulation...
  29. ncbi request reprint p53 mutant mice that display early ageing-associated phenotypes
    Stuart D Tyner
    Cell and Molecular Biology Program, Baylor College of Medicine, Houston, TX 77030, USA
    Nature 415:45-53. 2002
    ..A second line of transgenic mice containing a temperature-sensitive mutant allele of p53 also exhibits early ageing phenotypes. These data suggest that p53 has a role in regulating organismal ageing...
  30. ncbi request reprint Tools for targeted manipulation of the mouse genome
    Louise van der Weyden
    The Wellcome Trust Sanger Institute, Hinxton, Cambs CB10 1SA, United Kingdom
    Physiol Genomics 11:133-64. 2002
    ....
  31. pmc Disruption of the caveolin-1 gene impairs renal calcium reabsorption and leads to hypercalciuria and urolithiasis
    Guangwen Cao
    Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Pathol 162:1241-8. 2003
    ..These results demonstrate that disruption of the Cav1 gene promotes the progressive steps required for urinary calcium stone formation and establish a new mouse model for urinary stone disease...
  32. ncbi request reprint Two new balancer chromosomes on mouse chromosome 4 to facilitate functional annotation of human chromosome 1p
    Ichiko Nishijima
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Genesis 36:142-8. 2003
    ..These inversion strains will be extremely useful for mutagenesis screens that focus on functional annotation of human chromosome 1p...
  33. pmc The knockout mouse project
    Christopher P Austin
    National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA
    Nat Genet 36:921-4. 2004
    ..It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain...
  34. ncbi request reprint Reduced penetrance of craniofacial anomalies as a function of deletion size and genetic background in a chromosome engineered partial mouse model for Smith-Magenis syndrome
    Jiong Yan
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Hum Mol Genet 13:2613-24. 2004
    ..Our mouse models refined the genomic region important for a portion of the SMS phenotype and provided a basis for further molecular analysis of genes associated with SMS...
  35. ncbi request reprint Allelic phasing of a mouse chromosome 11 deficiency influences p53 tumorigenicity
    Patrick J Biggs
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs CB10 1SA, UK
    Oncogene 22:3288-96. 2003
    ..Even though the genotype of these mice is identical, allelic phasing affects both the tumour spectrum and progression...
  36. pmc Segmental trisomy of chromosome 17: a mouse model of human aneuploidy syndromes
    Tomas Vacik
    Institutes of Molecular Genetics and Physiology, Academy of Sciences of the Czech Republic, 14220 Prague, Czech Republic
    Proc Natl Acad Sci U S A 102:4500-5. 2005
    ..We propose that systemic comparisons of unrelated segmental trisomies, such as Ts65Dn and Ts43H, will elucidate the pathways leading from the triplicated sequences to the complex developmental traits...
  37. ncbi request reprint Null and conditional semaphorin 3B alleles using a flexible puroDeltatk loxP/FRT vector
    Louise van der Weyden
    Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom
    Genesis 41:171-8. 2005
    ..This targeting vector system has broad applicability in any knockout experiment and provides a flexible approach for the generation of modified alleles in mice...
  38. ncbi request reprint A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction
    David J Adams
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK
    Genomics 86:753-8. 2005
    ..Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines...
  39. ncbi request reprint Novel lethal mouse mutants produced in balancer chromosome screens
    Kathryn E Hentges
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Gene Expr Patterns 6:653-65. 2006
    ....
  40. pmc A deficiency in the region homologous to human 17q21.33-q23.2 causes heart defects in mice
    Y Eugene Yu
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 173:297-307. 2006
    ..Therefore, we have identified a new dosage-sensitive genomic region that may be critical for normal heart development in both mice and humans...
  41. ncbi request reprint Generation and maintenance of Dmbx1 gene-targeted mutant alleles
    Akihira Ohtoshi
    Center of Molecular and Human Genetics, Children s Research Institute, 700 Children s Drive, Columbus, Ohio 43205, USA
    Mamm Genome 17:744-50. 2006
    ..To our knowledge, this is the first demonstration of the proof-of-principle of the maintenance of viable gene-targeted alleles using coat-color-tagged nonlethal balancer chromosomes...
  42. pmc Requirement of bic/microRNA-155 for normal immune function
    Antony Rodriguez
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 316:608-11. 2007
    ..Our work suggests that bic/microRNA-155 plays a key role in the homeostasis and function of the immune system...

Research Grants5

  1. SCREEN FOR TUMOR SUPPRESSORS ON MOUSE CHROMOSOME 4
    Allan Bradley; Fiscal Year: 2001
    ..The pro-viral tag will facilitate the molecular identification of insertionally inactivated tumor suppressor genes in these cell lines. ..