Allan Bradley

Summary

Affiliation: Wellcome Trust Genome Campus
Country: UK

Publications

  1. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
  2. pmc Generation of an inducible and optimized piggyBac transposon system
    Juan Cadinanos
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    Nucleic Acids Res 35:e87. 2007
  3. pmc Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 478:391-4. 2011
  4. pmc Mobilization of giant piggyBac transposons in the mouse genome
    Meng Amy Li
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, CB10 1SA
    Nucleic Acids Res 39:e148. 2011
  5. pmc The mammalian gene function resource: the International Knockout Mouse Consortium
    Allan Bradley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
    Mamm Genome 23:580-6. 2012
  6. pmc A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells
    Wei Wang
    Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, PR China
    Genome Biol 8:R48. 2007
  7. pmc A mouse chromosome 4 balancer ENU-mutagenesis screen isolates eleven lethal lines
    Melissa K Boles
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    BMC Genet 10:12. 2009
  8. pmc PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice
    Roland Rad
    Wellcome Trust Sanger Institute, Genome Campus, Hinxton Cambridge CB10 1SA, UK
    Science 330:1104-7. 2010
  9. pmc Olfactory bulb hypoplasia in Prokr2 null mice stems from defective neuronal progenitor migration and differentiation
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Eur J Neurosci 26:3339-44. 2007
  10. pmc A DNA transposon-based approach to validate oncogenic mutations in the mouse
    Qin Su
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, England
    Proc Natl Acad Sci U S A 105:19904-9. 2008

Research Grants

Collaborators

Detail Information

Publications73

  1. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
    ..iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon-based reprogramming may be used to generate therapeutically applicable iPSCs...
  2. pmc Generation of an inducible and optimized piggyBac transposon system
    Juan Cadinanos
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
    Nucleic Acids Res 35:e87. 2007
    ..We expect that these tools, adaptable to perform mouse-germline mutagenesis, will facilitate the identification of genes involved in pathological and physiological processes, such as cancer or ES cell differentiation...
  3. pmc Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 478:391-4. 2011
    ..Our results provide the first proof of principle, to our knowledge, for the potential of combining human iPSCs with genetic correction to generate clinically relevant cells for autologous cell-based therapies...
  4. pmc Mobilization of giant piggyBac transposons in the mouse genome
    Meng Amy Li
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, CB10 1SA
    Nucleic Acids Res 39:e148. 2011
    ..Furthermore, these 100-kb transposons can also be excised from the genome without leaving a footprint. The development of piggyBac as a large cargo vector will facilitate a wider range of genetic and genomic applications...
  5. pmc The mammalian gene function resource: the International Knockout Mouse Consortium
    Allan Bradley
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
    Mamm Genome 23:580-6. 2012
    ..The IKMC materials considerably enhance functional gene annotation of the mammalian genome and will have a major impact on future biomedical research...
  6. pmc A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells
    Wei Wang
    Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing, PR China
    Genome Biol 8:R48. 2007
    ..In order to identify the host factors involved in retroviral infection, we designed and implemented a scheme for identifying ES cells that are resistant to retroviral infection and subsequent cloning of the mutated gene...
  7. pmc A mouse chromosome 4 balancer ENU-mutagenesis screen isolates eleven lethal lines
    Melissa K Boles
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    BMC Genet 10:12. 2009
    ..To functionally annotate the distal region of mouse chromosome 4, we performed an ENU-mutagenesis screen using a balancer chromosome targeted to this region of the genome...
  8. pmc PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice
    Roland Rad
    Wellcome Trust Sanger Institute, Genome Campus, Hinxton Cambridge CB10 1SA, UK
    Science 330:1104-7. 2010
    ..Mice with different transposon types, copy numbers, and chromosomal locations support wide applicability...
  9. pmc Olfactory bulb hypoplasia in Prokr2 null mice stems from defective neuronal progenitor migration and differentiation
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Eur J Neurosci 26:3339-44. 2007
    ..Together, these findings suggest an important role for Prokr2 in OB neurogenesis...
  10. pmc A DNA transposon-based approach to validate oncogenic mutations in the mouse
    Qin Su
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, England
    Proc Natl Acad Sci U S A 105:19904-9. 2008
    ..Consequently, these mice developed a broad spectrum of tumors at very early postnatal stages. This technology thus complements the large-scale cancer genome projects...
  11. pmc A conditional knockout resource for the genome-wide study of mouse gene function
    William C Skarnes
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 474:337-42. 2011
    ....
  12. pmc The piggyBac transposon displays local and distant reintegration preferences and can cause mutations at noncanonical integration sites
    Meng Amy Li
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Mol Cell Biol 33:1317-30. 2013
    ..Transposons could be mobilized from plasmids with the observed noncanonical flanking regions, indicating that piggyBac could generate point mutations in the genome...
  13. ncbi request reprint Mutagenic insertion and chromosome engineering resource (MICER)
    David J Adams
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs, CB10 1SA, UK
    Nat Genet 36:867-71. 2004
    ..These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome...
  14. pmc Agouti C57BL/6N embryonic stem cells for mouse genetic resources
    Stephen J Pettitt
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK
    Nat Methods 6:493-5. 2009
    ..These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background...
  15. ncbi request reprint microRNA-155 regulates the generation of immunoglobulin class-switched plasma cells
    Elena Vigorito
    Laboratory of Lymphocyte Signalling and Development, The Babraham Institute, Cambridge, CB22 3AT, UK
    Immunity 27:847-59. 2007
    ..1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells...
  16. pmc Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system
    Yue Huang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nucleic Acids Res 40:e21. 2012
    ..We obtained homozygous cells from 40% of the heterozygous mutants tested. This method can provide homozygous mammalian loss-of-function mutants for forward genetic applications...
  17. pmc Loss of TSLC1 causes male infertility due to a defect at the spermatid stage of spermatogenesis
    Louise van der Weyden
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Mol Cell Biol 26:3595-609. 2006
    ..Taken together, these data show that Tslc1 is essential for normal spermatogenesis in mice...
  18. pmc Mosaic complementation demonstrates a regulatory role for myosin VIIa in actin dynamics of stereocilia
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Mol Cell Biol 28:1702-12. 2008
    ..Our results strongly suggest that myosin VIIa regulates the establishment of a setpoint for stereocilium heights, and this novel role may influence their normal staircase-like arrangement within a bundle...
  19. pmc A hyperactive piggyBac transposase for mammalian applications
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 108:1531-6. 2011
    ..This hyperactive piggyBac transposase expands the utility of the piggyBac transposon for applications in mammalian genetics and gene therapy...
  20. pmc Genetic screens using the piggyBac transposon
    Su Kit Chew
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK
    Methods 53:366-71. 2011
    ..We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments...
  21. pmc Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  22. ncbi request reprint A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction
    David J Adams
    The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK
    Genomics 86:753-8. 2005
    ..Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines...
  23. pmc Induced mitotic recombination of p53 in vivo
    Wei Wang
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 104:4501-5. 2007
    ..Our results suggest that inducible mitotic recombination can be used for clonal analysis of mutants in the mouse...
  24. pmc Mutant nucleophosmin and cooperating pathways drive leukemia initiation and progression in mice
    George S Vassiliou
    Mouse Genomics Team, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Genet 43:470-5. 2011
    ..We also identified recurrent integrations in known and newly discovered leukemia genes including Nf1, Bach2, Dleu2 and Nup98. Our results provide new pathogenetic insights and identify possible therapeutic targets in NPM1c+ AML...
  25. ncbi request reprint Complex haplotypes, copy number polymorphisms and coding variation in two recently divergent mouse strains
    David J Adams
    The Wellcome Trust Sanger Institute, Hinxton, Cambs, CB10 1SA, UK
    Nat Genet 37:532-6. 2005
    ..Collectively, these genomic changes denote the level and direction of allele fixation that has occurred during inbreeding and provide a basis for defining what makes these mouse strains unique...
  26. pmc Functional knockout of the matrilin-3 gene causes premature chondrocyte maturation to hypertrophy and increases bone mineral density and osteoarthritis
    Louise van der Weyden
    Mouse Genomics Lab, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Am J Pathol 169:515-27. 2006
    ..The lack of Matn3 does not lead to postnatal chondrodysplasia but accounts for higher incidence of osteoarthritis...
  27. ncbi request reprint Mining the mouse genome
    Allan Bradley
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Nature 420:512-4. 2002
  28. pmc A resource of vectors and ES cells for targeted deletion of microRNAs in mice
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Biotechnol 29:840-5. 2011
    ..This miRNA knockout (mirKO) resource can be searched electronically and is available from ES cell repositories for distribution to the scientific community...
  29. pmc An expanded Oct4 interaction network: implications for stem cell biology, development, and disease
    Mercedes Pardo
    Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK
    Cell Stem Cell 6:382-95. 2010
    ..The Oct4 interactome provides a resource for dissecting mechanisms of Oct4 function, enlightening the basis of pluripotency and development, and identifying potential additional reprogramming factors...
  30. ncbi request reprint Null and conditional semaphorin 3B alleles using a flexible puroDeltatk loxP/FRT vector
    Louise van der Weyden
    Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom
    Genesis 41:171-8. 2005
    ..This targeting vector system has broad applicability in any knockout experiment and provides a flexible approach for the generation of modified alleles in mice...
  31. doi request reprint The use of DNA transposons for cancer gene discovery in mice
    George Vassiliou
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Methods Enzymol 477:91-106. 2010
    ..This chapter gives an overview of the design and application of transposons to cancer gene discovery in mice...
  32. doi request reprint Genome-wide forward genetic screens in mouse ES cells
    Meng Amy Li
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Methods Enzymol 477:217-42. 2010
    ....
  33. pmc Irradiated Blm-deficient mice are a highly tumor prone model for analysis of a broad spectrum of hematologic malignancies
    Madhuri Warren
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Leuk Res 34:210-20. 2010
    ..Irradiated Blm-deficient mice thus provide a novel model for understanding accelerated malignancies in BS and a new platform for investigating the molecular basis for a wide range of hematopoietic neoplasms...
  34. pmc Requirement of bic/microRNA-155 for normal immune function
    Antony Rodriguez
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Science 316:608-11. 2007
    ..Our work suggests that bic/microRNA-155 plays a key role in the homeostasis and function of the immune system...
  35. pmc A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 19:667-73. 2009
    ..The genomic coverage in this library confirms its utility as a new genetic resource for conducting recessive genetic screens in mammalian cells...
  36. pmc Prokineticin receptor 2 (Prokr2) is essential for the regulation of circadian behavior by the suprachiasmatic nuclei
    Haydn M Prosser
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 104:648-53. 2007
    ....
  37. ncbi request reprint Transgenics at breaking-point
    Haydn Prosser
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Cancer Cell 3:411-3. 2003
    ..2003) have generated mice that recapitulate both the mechanism (sporadic somatic translocation) and the consequences (expression of two translocation fusion genes) leading to an accurate leukemia model...
  38. pmc Extensive genomic copy number variation in embryonic stem cells
    Qi Liang
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:17453-6. 2008
    ..Human ES (hES) cells and derived somatic lineages may be similarly affected, challenging the concept of a stable somatic genome...
  39. pmc Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes
    Jacqueline K White
    Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Cell 154:452-64. 2013
    ..Phenotypic data and more than 900 mutants are openly available for further analysis. PAPERCLIP: ..
  40. pmc A practical and efficient cellular substrate for the generation of induced pluripotent stem cells from adults: blood-derived endothelial progenitor cells
    Imbisaat Geti
    University of Cambridge, Cambridge, UK
    Stem Cells Transl Med 1:855-65. 2012
    ..This work identifies L-EPCs as a practical and efficient cellular substrate for iPSC generation, with the potential to address many of the factors currently limiting the translation of this technology...
  41. pmc A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization
    Yeun Jun Chung
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Genome Res 14:188-96. 2004
    ..The resulting 3K mouse BAC microarrays reproducibly identified DNA copy number alterations in cell lines and primary tumors, such as single-copy deletions, regional amplifications, and aneuploidy...
  42. ncbi request reprint Chromosome engineering in ES cells
    Louise van der Weyden
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
    Methods Mol Biol 530:49-77. 2009
    ..The resulting mouse models are leading to a better understanding of the molecular and cellular basis of dosage alterations in human disease phenotypes, in turn opening new diagnostic and therapeutic opportunities...
  43. ncbi request reprint Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells
    Ge Guo
    The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
    Nature 429:891-5. 2004
    ..The combination of insertional mutagenesis in Blm-deficient ES cells establishes a new approach for phenotype-based recessive genetic screens in ES cells...
  44. pmc Mannosidase 2, alpha 1 deficiency is associated with ricin resistance in embryonic stem (ES) cells
    Wei Wang
    The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom
    PLoS ONE 6:e22993. 2011
    ..Therefore, we conclusively identified mannosidase 2, alpha 1 deficiency to be associated with ricin resistance...
  45. pmc Mouse chromosome engineering for modeling human disease
    Louise van der Weyden
    Mouse Genomics Lab, Wellcome Trust Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Annu Rev Genomics Hum Genet 7:247-76. 2006
    ..The resulting mouse models are leading to a better understanding of the molecular and cellular basis of dosage alterations in human disease phenotypes, in turn opening new diagnostic and therapeutic opportunities...
  46. ncbi request reprint Allelic phasing of a mouse chromosome 11 deficiency influences p53 tumorigenicity
    Patrick J Biggs
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambs CB10 1SA, UK
    Oncogene 22:3288-96. 2003
    ..Even though the genotype of these mice is identical, allelic phasing affects both the tumour spectrum and progression...
  47. ncbi request reprint Chromosomal mobilization and reintegration of Sleeping Beauty and PiggyBac transposons
    Qi Liang
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
    Genesis 47:404-8. 2009
    ..genesis 47:404-408, 2009. (c) 2009 Wiley-Liss, Inc...
  48. ncbi request reprint Tools for targeted manipulation of the mouse genome
    Louise van der Weyden
    The Wellcome Trust Sanger Institute, Hinxton, Cambs CB10 1SA, United Kingdom
    Physiol Genomics 11:133-64. 2002
    ....
  49. pmc Identification of mammalian microRNA host genes and transcription units
    Antony Rodriguez
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom
    Genome Res 14:1902-10. 2004
    ....
  50. pmc Genetic background drives transcriptional variation in human induced pluripotent stem cells
    Foad Rouhani
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom
    PLoS Genet 10:e1004432. 2014
    ..Our data also suggest that future studies using hIPSCs as a model system should focus most effort on collection of large numbers of donors, rather than generating large numbers of lines from the same donor. ..
  51. pmc Modeling del(17)(p11.2p11.2) and dup(17)(p11.2p11.2) contiguous gene syndromes by chromosome engineering in mice: phenotypic consequences of gene dosage imbalance
    Katherina Walz
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Cell Biol 23:3646-55. 2003
    ..Our murine models represent a powerful tool to analyze the consequences of gene dosage imbalance in this genomic interval and to investigate the molecular genetic bases of both SMS and dup(17)(p11.2p11.2)...
  52. pmc Urocortin-deficient mice display normal stress-induced anxiety behavior and autonomic control but an impaired acoustic startle response
    Xiaozhong Wang
    Department of Molecular and Human Genetics, Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:6605-10. 2002
    ..Ucn may modulate the acoustic startle response through the Ucn-expressing neuron projections from the region of the Edinger-Westphal nucleus...
  53. pmc Molecular mechanisms governing Pcdh-gamma gene expression: evidence for a multiple promoter and cis-alternative splicing model
    Xiaozhong Wang
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 16:1890-905. 2002
    ....
  54. ncbi request reprint A targeted X-linked CMV-Cre line
    Hong Su
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Genesis 32:187-8. 2002
  55. ncbi request reprint Visual genotyping of a coat color tagged p53 mutant mouse line
    Binhai Zheng
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Cancer Biol Ther 1:433-5. 2002
    ..Thus, tagging targeted mutations with such coat color markers provides a generally applicable genotyping method for embryonic stem cell-derived mice...
  56. pmc Inducible gene trapping with drug-selectable markers and Cre/loxP to identify developmentally regulated genes
    You Tzung Chen
    Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, USA
    Mol Cell Biol 24:9930-41. 2004
    ..Furthermore, we demonstrate that the cre reporter is extremely sensitive and can be used to explore chromosomal regions that are not detectable with neo as a selection cassette...
  57. pmc Bigenic Cre/loxP, puDeltatk conditional genetic ablation
    You Tzung Chen
    Program in Developmental Biology, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Nucleic Acids Res 32:e161. 2004
    ..5 dpc embryos. Crosses between the puDeltatk selector transgenic line and existing cre lines will facilitate numerous temporally regulated tissue-specific ablation experiments...
  58. ncbi request reprint Induced mitotic recombination: a switch in time
    David J Adams
    Nat Genet 30:6-7. 2002
  59. ncbi request reprint Two new mouse chromosome 11 balancers
    Jan Klysik
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Genomics 83:303-10. 2004
    ..With the addition of these balancers to the previously reported Trp53-Wnt3 balancer, most of mouse chromosome 11 is now available in balancer stocks...
  60. pmc A deficiency in the region homologous to human 17q21.33-q23.2 causes heart defects in mice
    Y Eugene Yu
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Genetics 173:297-307. 2006
    ..Therefore, we have identified a new dosage-sensitive genomic region that may be critical for normal heart development in both mice and humans...
  61. ncbi request reprint p53 mutant mice that display early ageing-associated phenotypes
    Stuart D Tyner
    Cell and Molecular Biology Program, Baylor College of Medicine, Houston, TX 77030, USA
    Nature 415:45-53. 2002
    ..A second line of transgenic mice containing a temperature-sensitive mutant allele of p53 also exhibits early ageing phenotypes. These data suggest that p53 has a role in regulating organismal ageing...
  62. pmc Mice deficient for the wild-type p53-induced phosphatase gene (Wip1) exhibit defects in reproductive organs, immune function, and cell cycle control
    Jene Choi
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:1094-105. 2002
    ..The data are consistent with an important role for Wip1 in spermatogenesis, lymphoid cell function, and cell cycle regulation...
  63. ncbi request reprint Conditional inactivation of p63 by Cre-mediated excision
    Alea A Mills
    Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Genesis 32:138-41. 2002
  64. pmc Segmental trisomy of chromosome 17: a mouse model of human aneuploidy syndromes
    Tomas Vacik
    Institutes of Molecular Genetics and Physiology, Academy of Sciences of the Czech Republic, 14220 Prague, Czech Republic
    Proc Natl Acad Sci U S A 102:4500-5. 2005
    ..We propose that systemic comparisons of unrelated segmental trisomies, such as Ts65Dn and Ts43H, will elucidate the pathways leading from the triplicated sequences to the complex developmental traits...
  65. ncbi request reprint Novel lethal mouse mutants produced in balancer chromosome screens
    Kathryn E Hentges
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Gene Expr Patterns 6:653-65. 2006
    ....
  66. ncbi request reprint Two new balancer chromosomes on mouse chromosome 4 to facilitate functional annotation of human chromosome 1p
    Ichiko Nishijima
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Genesis 36:142-8. 2003
    ..These inversion strains will be extremely useful for mutagenesis screens that focus on functional annotation of human chromosome 1p...
  67. pmc Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster
    Ulrike C Lange
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Mol Cell Biol 28:4688-96. 2008
    ..Hence, contrary to previous reports, Ifitm genes are not essential for PGC migration. The Ifitm family is a striking example of a conserved gene cluster which appears to be functionally redundant during development...
  68. pmc Increased insulin action in SKIP heterozygous knockout mice
    Takeshi Ijuin
    Division of Lipid Biochemistry, Kobe University Graduate School of Medicine, Kobe 650 0017, Hyogo, Japan
    Mol Cell Biol 28:5184-95. 2008
    ..These results imply that SKIP regulates insulin signaling in skeletal muscle. Thus, SKIP may be a promising pharmacologic target for the treatment of insulin resistance and diabetes...
  69. ncbi request reprint Reduced penetrance of craniofacial anomalies as a function of deletion size and genetic background in a chromosome engineered partial mouse model for Smith-Magenis syndrome
    Jiong Yan
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Hum Mol Genet 13:2613-24. 2004
    ..Our mouse models refined the genomic region important for a portion of the SMS phenotype and provided a basis for further molecular analysis of genes associated with SMS...
  70. pmc The knockout mouse project
    Christopher P Austin
    National Human Genome Research Institute, National Institutes of Health, Building 31, Room 4B09, 31 Center Drive, Bethesda, Maryland 20892, USA
    Nat Genet 36:921-4. 2004
    ..It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain...
  71. pmc Disruption of the caveolin-1 gene impairs renal calcium reabsorption and leads to hypercalciuria and urolithiasis
    Guangwen Cao
    Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Pathol 162:1241-8. 2003
    ..These results demonstrate that disruption of the Cav1 gene promotes the progressive steps required for urinary calcium stone formation and establish a new mouse model for urinary stone disease...
  72. pmc Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy
    Fung Chan
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:9109-14. 2004
    ....
  73. ncbi request reprint Generation and maintenance of Dmbx1 gene-targeted mutant alleles
    Akihira Ohtoshi
    Center of Molecular and Human Genetics, Children s Research Institute, 700 Children s Drive, Columbus, Ohio 43205, USA
    Mamm Genome 17:744-50. 2006
    ..To our knowledge, this is the first demonstration of the proof-of-principle of the maintenance of viable gene-targeted alleles using coat-color-tagged nonlethal balancer chromosomes...

Research Grants5

  1. SCREEN FOR TUMOR SUPPRESSORS ON MOUSE CHROMOSOME 4
    Allan Bradley; Fiscal Year: 1999
    ..The pro-viral tag will facilitate the molecular identification of insertionally inactivated tumor suppressor genes in these cell lines. ..
  2. SCREEN FOR TUMOR SUPPRESSORS ON MOUSE CHROMOSOME 4
    Allan Bradley; Fiscal Year: 2000
    ..The pro-viral tag will facilitate the molecular identification of insertionally inactivated tumor suppressor genes in these cell lines. ..
  3. SCREEN FOR TUMOR SUPPRESSORS ON MOUSE CHROMOSOME 4
    Allan Bradley; Fiscal Year: 2001
    ..The pro-viral tag will facilitate the molecular identification of insertionally inactivated tumor suppressor genes in these cell lines. ..