Paul Robert Wheeler
Affiliation: Veterinary Laboratories Agency
- Analyzing lipid metabolism: activation and beta-oxidation of fatty acidsPaul Robert Wheeler
Veterinary Laboratories Agency Weybridge, Tuberculosis Research Group, New Haw, Addlestone, Surrey KT15 3NB, UK
Methods Mol Biol 465:47-59. 2009..Here we present methods for the cell-free assay of types of enzymes in live bacteria, as well as for fatty acid oxidation overall...
- Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivoEsen Wooff
Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Surrey, UK
Mol Microbiol 43:653-63. 2002..Hence, it appears that this is the sole locus encoding inorganic sulphur transport in the M. tuberculosis complex...
- GSMN-TB: a web-based genome-scale network model of Mycobacterium tuberculosis metabolismDany J V Beste
School of Biomedical and Molecular Sciences, University of Surrey, Stag Hill, Guildford, Surrey, GU2 7XH, UK
Genome Biol 8:R89. 2007..Constraint-based modeling provides a novel approach to investigating microbial metabolism but has not yet been applied to genome-scale modeling of M. tuberculosis...
- Inositol monophosphate phosphatase genes of Mycobacterium tuberculosisFarahnaz Movahedzadeh
Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
BMC Microbiol 10:50. 2010..To gain insight into how Mycobacterium tuberculosis synthesises inositol we carried out genetic analysis of the four IMPase homologues that are present in the Mycobacterium tuberculosis genome...
- Analysis of lipid biosynthesis and locationPaul Robert Wheeler
Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
Methods Mol Biol 465:61-81. 2009..A standardized, TLC-based method for general lipid analysis that can be used to quantify the labeling of all the mycobacterial lipids is given as well as a protocol for analyzing the fatty acyl moieties of the lipids...
- Functional analysis of a clonal deletion in an epidemic strain of Mycobacterium bovis reveals a role in lipid metabolismPaul R Wheeler
VLA Weybridge, New Haw, Addlestone KT15 3NB, Surrey, UK
Microbiology 154:3731-42. 2008..bovis (but not in M. tuberculosis). This shift in carbon metabolism may be a factor in the extraordinary clonal expansion reported for M. bovis type 17...
- Functional demonstration of reverse transsulfuration in the Mycobacterium tuberculosis complex reveals that methionine is the preferred sulfur source for pathogenic MycobacteriaPaul R Wheeler
Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom
J Biol Chem 280:8069-78. 2005..Cysteine desulfhydrase, an activity we showed to be separable from CGL/CGS, may have a role in removing excess cysteine and could explain the ability of M. tuberculosis to recycle sulfur from cysteine, but not methionine...
- Global effects of inactivation of the pyruvate kinase gene in the Mycobacterium tuberculosis complexSivagamisundaram Chavadi
Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Surrey, KT15 3NB, United Kingdom
J Bacteriol 191:7545-53. 2009..tuberculosis DeltapykA Deltaald double mutant showed little additional phenotypic effect. Overall, these data show that the loss of the pykA gene has powerful, global effects on proteins associated with central metabolism...
- The pyruvate requirement of some members of the Mycobacterium tuberculosis complex is due to an inactive pyruvate kinase: implications for in vivo growthLisa A Keating
TB Research Group, Veterinary Laboratories Agency Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
Mol Microbiol 56:163-74. 2005..In summary, the disruption of a key step in glycolysis divides the M. tuberculosis complex into two groups with distinct carbon source utilization...
- The MPB83 antigen from Mycobacterium bovis contains O-linked mannose and (1-->3)-mannobiose moietiesStephen L Michell
TB Research Group, Department of Bacterial Diseases, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
J Biol Chem 278:16423-32. 2003..Given such a possibility characterization of mycobacterial glycoproteins is a step toward understanding their functional role and elucidating the mechanisms of mycobacterial glycosylation...
- The complete genome sequence of Mycobacterium bovisThierry Garnier
, , Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
Proc Natl Acad Sci U S A 100:7877-82. 2003..bovis, implying that differential gene expression may be the key to the host tropisms of human and bovine bacilli. The genome sequence therefore offers major insight on the evolution, host preference, and pathobiology of M. bovis...
- Biological implications of Mycobacterium leprae gene expression during infectionDiana L Williams
Laboratory Research Branch, Division of the National Hansen s Disease Programs at LSU SVM, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USA
J Mol Microbiol Biotechnol 8:58-72. 2004..leprae gene expression profiling. Identifying genes associated with growth and survival during infection should lead to a more comprehensive understanding of the ability of M. leprae to cause disease...
- Tubercle bacilli generate a novel cell wall-associated pigment after long-term anaerobic cultureAdam F Cunningham
Birmingham Centre for Immune Regulation, University of Birmingham, Birmingham B15 2TT, UK
FEMS Microbiol Lett 235:191-8. 2004..We termed this pigment APP1, anaerobically produced pigment 1, the first pigment identified in M. tuberculosis...
- Transposon mutagenesis of Mb0100 at the ppe1-nrp locus in Mycobacterium bovis disrupts phthiocerol dimycocerosate (PDIM) and glycosylphenol-PDIM biosynthesis, producing an avirulent strain with vaccine properties at least equal to those of M. bovis BCGGrant S Hotter
AgResearch, Wallaceville Animal Research Centre, P O Box 40063, Upper Hutt, New Zealand
J Bacteriol 187:2267-77. 2005..Both mutants are avirulent in a guinea pig model of tuberculosis, and when tested as a vaccine, WAg533 conferred protective immunity against M. bovis infection at least equal to that afforded by M. bovis bacillus Calmette-Guerin...