Paul Robert Wheeler

Summary

Affiliation: Veterinary Laboratories Agency
Country: UK

Publications

  1. doi request reprint Analyzing lipid metabolism: activation and beta-oxidation of fatty acids
    Paul Robert Wheeler
    Veterinary Laboratories Agency Weybridge, Tuberculosis Research Group, New Haw, Addlestone, Surrey KT15 3NB, UK
    Methods Mol Biol 465:47-59. 2009
  2. ncbi request reprint Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo
    Esen Wooff
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Surrey, UK
    Mol Microbiol 43:653-63. 2002
  3. pmc GSMN-TB: a web-based genome-scale network model of Mycobacterium tuberculosis metabolism
    Dany J V Beste
    School of Biomedical and Molecular Sciences, University of Surrey, Stag Hill, Guildford, Surrey, GU2 7XH, UK
    Genome Biol 8:R89. 2007
  4. pmc Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis
    Farahnaz Movahedzadeh
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    BMC Microbiol 10:50. 2010
  5. doi request reprint Analysis of lipid biosynthesis and location
    Paul Robert Wheeler
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
    Methods Mol Biol 465:61-81. 2009
  6. doi request reprint Functional analysis of a clonal deletion in an epidemic strain of Mycobacterium bovis reveals a role in lipid metabolism
    Paul R Wheeler
    VLA Weybridge, New Haw, Addlestone KT15 3NB, Surrey, UK
    Microbiology 154:3731-42. 2008
  7. ncbi request reprint Functional demonstration of reverse transsulfuration in the Mycobacterium tuberculosis complex reveals that methionine is the preferred sulfur source for pathogenic Mycobacteria
    Paul R Wheeler
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom
    J Biol Chem 280:8069-78. 2005
  8. pmc Global effects of inactivation of the pyruvate kinase gene in the Mycobacterium tuberculosis complex
    Sivagamisundaram Chavadi
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Surrey, KT15 3NB, United Kingdom
    J Bacteriol 191:7545-53. 2009
  9. ncbi request reprint The pyruvate requirement of some members of the Mycobacterium tuberculosis complex is due to an inactive pyruvate kinase: implications for in vivo growth
    Lisa A Keating
    TB Research Group, Veterinary Laboratories Agency Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
    Mol Microbiol 56:163-74. 2005
  10. ncbi request reprint The MPB83 antigen from Mycobacterium bovis contains O-linked mannose and (1-->3)-mannobiose moieties
    Stephen L Michell
    TB Research Group, Department of Bacterial Diseases, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
    J Biol Chem 278:16423-32. 2003

Collaborators

  • S T Cole
  • D B Young
  • R Glyn Hewinson
  • Stephen V Gordon
  • Tanya Parish
  • Mark Andrew Chambers
  • Adam F Cunningham
  • D M Collins
  • Claire L Spreadbury
  • Adam O Whelan
  • Esen Wooff
  • Farahnaz Movahedzadeh
  • Sivagamisundaram Chavadi
  • Dany J V Beste
  • Lisa A Keating
  • Grant S Hotter
  • Huma Mansoor
  • Diana L Williams
  • Thierry Garnier
  • Stephen L Michell
  • Premkumar Dinadayala
  • Yossef Av-Gay
  • Mamadou Daffe
  • Neil G Stoker
  • Manjula Sritharan
  • Nicholas G Coldham
  • Bhushan Bonde
  • Steffen Klamt
  • Claudio Avignone-Rossa
  • Tracy Hooper
  • Andrzej M Kierzek
  • Johnjoe McFadden
  • Michael E Bushell
  • Graham Stewart
  • Jessica Gomes
  • Pamela Kawakami
  • James Dale
  • Jacqueline K Inwald
  • Barry J Wards
  • Pania Mouat
  • Gurdyal S Besra
  • Shalome Bassett
  • Monica Singh
  • Geoffrey W de Lisle
  • Mark Yoder
  • Marina Torrero
  • William R Bishai
  • Thomas P Gillis
  • Richard W Truman
  • Norman Morrison
  • Howard R Morris
  • Sylvie Simon
  • Jon Doggett
  • A Tony Etienne
  • Andrew J Reason
  • Sophie Grondin
  • Stephanie Duthoy
  • Anne Dell
  • Bart G Barrell
  • Karin Eiglmeier
  • Julian Parkhill
  • Rebecca Mayes
  • Richard L Easton
  • Celine Lacroix
  • Melinda Pryor
  • Rebecca Atkin
  • Lisa Keating
  • Maria Panico
  • Jean Christophe Camus
  • Christel Monsempe
  • Nadine Medina
  • Jean Louis Herrmann
  • Stuart M Haslam
  • Barbara Harris
  • William R Jacobs
  • Stoyan Bardarov
  • Stephen L I Michell

Detail Information

Publications14

  1. doi request reprint Analyzing lipid metabolism: activation and beta-oxidation of fatty acids
    Paul Robert Wheeler
    Veterinary Laboratories Agency Weybridge, Tuberculosis Research Group, New Haw, Addlestone, Surrey KT15 3NB, UK
    Methods Mol Biol 465:47-59. 2009
    ..Here we present methods for the cell-free assay of types of enzymes in live bacteria, as well as for fatty acid oxidation overall...
  2. ncbi request reprint Functional genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo
    Esen Wooff
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Surrey, UK
    Mol Microbiol 43:653-63. 2002
    ..Hence, it appears that this is the sole locus encoding inorganic sulphur transport in the M. tuberculosis complex...
  3. pmc GSMN-TB: a web-based genome-scale network model of Mycobacterium tuberculosis metabolism
    Dany J V Beste
    School of Biomedical and Molecular Sciences, University of Surrey, Stag Hill, Guildford, Surrey, GU2 7XH, UK
    Genome Biol 8:R89. 2007
    ..Constraint-based modeling provides a novel approach to investigating microbial metabolism but has not yet been applied to genome-scale modeling of M. tuberculosis...
  4. pmc Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis
    Farahnaz Movahedzadeh
    Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, London NW1 0TU, UK
    BMC Microbiol 10:50. 2010
    ..To gain insight into how Mycobacterium tuberculosis synthesises inositol we carried out genetic analysis of the four IMPase homologues that are present in the Mycobacterium tuberculosis genome...
  5. doi request reprint Analysis of lipid biosynthesis and location
    Paul Robert Wheeler
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
    Methods Mol Biol 465:61-81. 2009
    ..A standardized, TLC-based method for general lipid analysis that can be used to quantify the labeling of all the mycobacterial lipids is given as well as a protocol for analyzing the fatty acyl moieties of the lipids...
  6. doi request reprint Functional analysis of a clonal deletion in an epidemic strain of Mycobacterium bovis reveals a role in lipid metabolism
    Paul R Wheeler
    VLA Weybridge, New Haw, Addlestone KT15 3NB, Surrey, UK
    Microbiology 154:3731-42. 2008
    ..bovis (but not in M. tuberculosis). This shift in carbon metabolism may be a factor in the extraordinary clonal expansion reported for M. bovis type 17...
  7. ncbi request reprint Functional demonstration of reverse transsulfuration in the Mycobacterium tuberculosis complex reveals that methionine is the preferred sulfur source for pathogenic Mycobacteria
    Paul R Wheeler
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom
    J Biol Chem 280:8069-78. 2005
    ..Cysteine desulfhydrase, an activity we showed to be separable from CGL/CGS, may have a role in removing excess cysteine and could explain the ability of M. tuberculosis to recycle sulfur from cysteine, but not methionine...
  8. pmc Global effects of inactivation of the pyruvate kinase gene in the Mycobacterium tuberculosis complex
    Sivagamisundaram Chavadi
    Tuberculosis Research Group, Veterinary Laboratories Agency Weybridge, New Haw, Surrey, KT15 3NB, United Kingdom
    J Bacteriol 191:7545-53. 2009
    ..tuberculosis DeltapykA Deltaald double mutant showed little additional phenotypic effect. Overall, these data show that the loss of the pykA gene has powerful, global effects on proteins associated with central metabolism...
  9. ncbi request reprint The pyruvate requirement of some members of the Mycobacterium tuberculosis complex is due to an inactive pyruvate kinase: implications for in vivo growth
    Lisa A Keating
    TB Research Group, Veterinary Laboratories Agency Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
    Mol Microbiol 56:163-74. 2005
    ..In summary, the disruption of a key step in glycolysis divides the M. tuberculosis complex into two groups with distinct carbon source utilization...
  10. ncbi request reprint The MPB83 antigen from Mycobacterium bovis contains O-linked mannose and (1-->3)-mannobiose moieties
    Stephen L Michell
    TB Research Group, Department of Bacterial Diseases, Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
    J Biol Chem 278:16423-32. 2003
    ..Given such a possibility characterization of mycobacterial glycoproteins is a step toward understanding their functional role and elucidating the mechanisms of mycobacterial glycosylation...
  11. pmc The complete genome sequence of Mycobacterium bovis
    Thierry Garnier
    Unité de Génétique Moléculaire Bactérienne and PT4 Annotation, Genopole, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 100:7877-82. 2003
    ..bovis, implying that differential gene expression may be the key to the host tropisms of human and bovine bacilli. The genome sequence therefore offers major insight on the evolution, host preference, and pathobiology of M. bovis...
  12. ncbi request reprint Biological implications of Mycobacterium leprae gene expression during infection
    Diana L Williams
    Laboratory Research Branch, Division of the National Hansen s Disease Programs at LSU SVM, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA 70803, USA
    J Mol Microbiol Biotechnol 8:58-72. 2004
    ..leprae gene expression profiling. Identifying genes associated with growth and survival during infection should lead to a more comprehensive understanding of the ability of M. leprae to cause disease...
  13. ncbi request reprint Tubercle bacilli generate a novel cell wall-associated pigment after long-term anaerobic culture
    Adam F Cunningham
    Birmingham Centre for Immune Regulation, University of Birmingham, Birmingham B15 2TT, UK
    FEMS Microbiol Lett 235:191-8. 2004
    ..We termed this pigment APP1, anaerobically produced pigment 1, the first pigment identified in M. tuberculosis...
  14. pmc Transposon mutagenesis of Mb0100 at the ppe1-nrp locus in Mycobacterium bovis disrupts phthiocerol dimycocerosate (PDIM) and glycosylphenol-PDIM biosynthesis, producing an avirulent strain with vaccine properties at least equal to those of M. bovis BCG
    Grant S Hotter
    AgResearch, Wallaceville Animal Research Centre, P O Box 40063, Upper Hutt, New Zealand
    J Bacteriol 187:2267-77. 2005
    ..Both mutants are avirulent in a guinea pig model of tuberculosis, and when tested as a vaccine, WAg533 conferred protective immunity against M. bovis infection at least equal to that afforded by M. bovis bacillus Calmette-Guerin...