Affiliation: University of the West of England
- Optimized real-time quantitative PCR measurement of male fetal DNA in maternal plasmaBernhard Zimmermann
University Women s Hospital Department of Research, University Hospital Basel, Basel, Switzerland
Clin Chem 51:1598-604. 2005..The circulating concentrations of single-copy fetal genes, however, are close to the detection limits of PCR methods...
- Real-time quantitative polymerase chain reaction measurement of male fetal DNA in maternal plasmaBernhard Zimmermann
University Women s Hospital Department of Research, University Hospital Basel, Switzerland
Methods Mol Med 132:43-9. 2007..This chapter presents the basic methodology used in this noninvasive analysis...
- Detection of paternally inherited fetal point mutations for beta-thalassemia using size-fractionated cell-free DNA in maternal plasmaYing Li
Laboratory for Prenatal Medicine, University Women's Hospital/Department of Research, Basel, Switzerland
JAMA 293:843-9. 2005..8%, with classified cases removed. CONCLUSION: Our recently described technique of the size-fractionation of circulatory DNA in maternal plasma may be potentially useful for the noninvasive prenatal determination of fetal point mutations...
- Size separation of circulatory DNA in maternal plasma permits ready detection of fetal DNA polymorphismsYing Li
University Women's Hospital/Department of Research, University of Basel, Basel, Switzerland
Clin Chem 50:1002-11. 2004..CONCLUSIONS: Circulatory fetal DNA can be enriched by size selection of fragment sizes less than approximately 0.3 kb. Such selection permits easier analysis of both paternally and maternally inherited DNA polymorphisms...
- Treatment of maternal blood samples with formaldehyde does not alter the proportion of circulatory fetal nucleic acids (DNA and mRNA) in maternal plasmaSatheesh Kumar Reddy Chinnapapagari
Department of Research, University Women's Hospital, Basel, Switzerland
Clin Chem 51:652-5. 2005
- Nucleic acid based biosensors: the desires of the userSinuhe Hahn
Laboratory for Prenatal Medicine, University Women s Hospital Department of Research, University of Basel, Spitalstrasse 21, CH 4031 Basel, Switzerland
Bioelectrochemistry 67:151-4. 2005..In this review, we provide an overview of the current state of the art concerning the high-throughput analysis of nucleic acids, and address future requirements, which will hopefully be met by new biosensor-based developments...
- Novel real-time quantitative PCR test for trisomy 21Bernhard Zimmermann
Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, CH 4031 Basel, Switzerland
Clin Chem 48:362-3. 2002
- Staphylococcus aureus infection caused by a Panton Valentine leukocidin-producing strainChristian Aepinus
Pediatr Infect Dis J 24:284-5. 2005
- Inability to clonally expand fetal progenitors from maternal bloodBernhard Zimmermann
Department of Obstetrics and Gynaecology, University of Basel, Switzerland
Fetal Diagn Ther 17:97-100. 2002..No colonies were detected of fetal origin. CONCLUSIONS: The clonal expansion of single fetal colonies from the maternal circulation is not feasible by current methods...
- Use of real-time polymerase chain reaction for the detection of fetal aneuploidiesBernhard Zimmermann
Centre for Research in Biomedicine, University of the West of England, Bristol, UK
Methods Mol Biol 336:83-100. 2006..5-fold differences in gene copy number...
- Reliable detection of trisomy 21 using MALDI-TOF mass spectrometryDorothy J Huang
Laboratory for Prenatal Medicine University Women's Hospital, Department of Research Spitalstrasse 21, CH4031 Basel, Switzerland
Genet Med 8:728-34. 2006..Its amenability to high-throughput analysis and high degree of multiplexing make it a potential future diagnostic tool for the detection of other aneuploidies as well...
- Molecular methods for rapid detection of aneuploidyLech Dudarewicz
Department of Medical Genetics, Polish Mother s Memorial Hospital Research Institute, Rzgowska 281 289, 93 338 Łódź, Poland
J Appl Genet 46:207-15. 2005....