K W Young

Summary

Affiliation: University of Leicester
Country: UK

Publications

  1. ncbi request reprint Determination of the inositol (1,4,5) trisphosphate requirement for histamine- and substance P-induced Ca2+ mobilisation in human U373 MG astrocytoma cells
    K W Young
    Department of Cell Physiology and Pharmacology, University of Leicester, UK
    Cell Calcium 24:59-70. 1998
  2. ncbi request reprint Role of Ca2+ feedback on single cell inositol 1,4,5-trisphosphate oscillations mediated by G-protein-coupled receptors
    Kenneth W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester LE1 9HN, United Kingdom
    J Biol Chem 278:20753-60. 2003
  3. doi request reprint Mitochondrial fragmentation and neuronal cell death in response to the Bcl-2/Bcl-x(L)/Bcl-w antagonist ABT-737
    Kenneth W Young
    MRC Toxicology Unit, University of Leicester, Hodgkin Building, Lancaster Road, Leicester LE1 9HN, UK
    Neuropharmacology 58:1258-67. 2010
  4. doi request reprint Different pathways lead to mitochondrial fragmentation during apoptotic and excitotoxic cell death in primary neurons
    Kenneth W Young
    MRC Toxicology Unit, University of Leicester, Leicester, LE1 9HN, UK
    J Biochem Mol Toxicol 24:335-41. 2010
  5. ncbi request reprint NMDA-receptor regulation of muscarinic-receptor stimulated inositol 1,4,5-trisphosphate production and protein kinase C activation in single cerebellar granule neurons
    Kenneth W Young
    Department of Cell Physiology and Pharmacology, University of Leicester, Medical Sciences Building, University Road, Leicester LE1 9HN, UK
    J Neurochem 89:1537-46. 2004
  6. ncbi request reprint Intracellular sphingosine 1-phosphate production: a novel pathway for Ca2+ release
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester, LE1 9HN, UK
    Semin Cell Dev Biol 12:19-25. 2001
  7. ncbi request reprint Effect of dimethylsphingosine on muscarinic M(3) receptor signalling in SH-SY5Y cells
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester LE1 9HN, UK
    Eur J Pharmacol 402:55-9. 2000
  8. pmc Lysophosphatidic acid-mediated Ca2+ mobilization in human SH-SY5Y neuroblastoma cells is independent of phosphoinositide signalling, but dependent on sphingosine kinase activation
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, P O Box 138, University Road, Leicester LE1 9HN, U K
    Biochem J 343:45-52. 1999
  9. ncbi request reprint Reassessment of the Ca2+ sensing property of a type I metabotropic glutamate receptor by simultaneous measurement of inositol 1,4,5-trisphosphate and Ca2+ in single cells
    M S Nash
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, P. O. Box 138, University Road, Leicester, LE1 9HN, United Kingdom
    J Biol Chem 276:19286-93. 2001
  10. pmc Single-cell imaging of graded Ins(1,4,5)P3 production following G-protein-coupled-receptor activation
    M S Nash
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, P O Box 138, University Road, Leicester LE1 9HN, UK
    Biochem J 356:137-42. 2001

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Determination of the inositol (1,4,5) trisphosphate requirement for histamine- and substance P-induced Ca2+ mobilisation in human U373 MG astrocytoma cells
    K W Young
    Department of Cell Physiology and Pharmacology, University of Leicester, UK
    Cell Calcium 24:59-70. 1998
    ..These results demonstrate that extremely small increases in Ins(1,4,5)P3 can stimulate large increases in [Ca2+]i in U373 MG cells, and suggest a marked redundancy for Ins(1,4,5)P3 production in the Ca2+ signalling pathway...
  2. ncbi request reprint Role of Ca2+ feedback on single cell inositol 1,4,5-trisphosphate oscillations mediated by G-protein-coupled receptors
    Kenneth W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester LE1 9HN, United Kingdom
    J Biol Chem 278:20753-60. 2003
    ....
  3. doi request reprint Mitochondrial fragmentation and neuronal cell death in response to the Bcl-2/Bcl-x(L)/Bcl-w antagonist ABT-737
    Kenneth W Young
    MRC Toxicology Unit, University of Leicester, Hodgkin Building, Lancaster Road, Leicester LE1 9HN, UK
    Neuropharmacology 58:1258-67. 2010
    ..Our data suggests that caspase-dependent cleavage of Bid may be a downstream amplification event which enhances the rate of mitochondrial fragmentation...
  4. doi request reprint Different pathways lead to mitochondrial fragmentation during apoptotic and excitotoxic cell death in primary neurons
    Kenneth W Young
    MRC Toxicology Unit, University of Leicester, Leicester, LE1 9HN, UK
    J Biochem Mol Toxicol 24:335-41. 2010
    ..In contrast, during glutamate excitotoxicity, Bax remained in the cytosolic compartment. We conclude that different pathways lead to the appearance of fragmented mitochondria during necrotic and apoptotic neuronal cell death...
  5. ncbi request reprint NMDA-receptor regulation of muscarinic-receptor stimulated inositol 1,4,5-trisphosphate production and protein kinase C activation in single cerebellar granule neurons
    Kenneth W Young
    Department of Cell Physiology and Pharmacology, University of Leicester, Medical Sciences Building, University Road, Leicester LE1 9HN, UK
    J Neurochem 89:1537-46. 2004
    ..Thus, NMDA-mediated Ca2+ influx and PLC activation may represent a coincident-detection system whereby ionotropic and metabotropic signals combine to stimulate InsP(3) production and PKC-mediated phosphorylation events in CGNs...
  6. ncbi request reprint Intracellular sphingosine 1-phosphate production: a novel pathway for Ca2+ release
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester, LE1 9HN, UK
    Semin Cell Dev Biol 12:19-25. 2001
    ..The functional significance of this novel Ca2+ release pathway is also discussed...
  7. ncbi request reprint Effect of dimethylsphingosine on muscarinic M(3) receptor signalling in SH-SY5Y cells
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester LE1 9HN, UK
    Eur J Pharmacol 402:55-9. 2000
    ..Thus, this compound must be used with caution when investigating the role of sphingosine kinase in G-protein coupled receptor-mediated Ca(2+) mobilisation responses...
  8. pmc Lysophosphatidic acid-mediated Ca2+ mobilization in human SH-SY5Y neuroblastoma cells is independent of phosphoinositide signalling, but dependent on sphingosine kinase activation
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, P O Box 138, University Road, Leicester LE1 9HN, U K
    Biochem J 343:45-52. 1999
    ..Furthermore, the Ca(2+)-response to LPA appears to be dependent on sphingosine kinase activation and the potential generation of the putative second messenger sphingosine 1-phosphate...
  9. ncbi request reprint Reassessment of the Ca2+ sensing property of a type I metabotropic glutamate receptor by simultaneous measurement of inositol 1,4,5-trisphosphate and Ca2+ in single cells
    M S Nash
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, P. O. Box 138, University Road, Leicester, LE1 9HN, United Kingdom
    J Biol Chem 276:19286-93. 2001
    ..However, prolonged activation of phospholipase C requires extracellular Ca(2+), while the initial burst of activity is highly dependent on Ca(2+) mobilization from intracellular stores...
  10. pmc Single-cell imaging of graded Ins(1,4,5)P3 production following G-protein-coupled-receptor activation
    M S Nash
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, P O Box 138, University Road, Leicester LE1 9HN, UK
    Biochem J 356:137-42. 2001
    ..Moreover, Ins(1,4,5)P(3) accumulation and intracellular Ca(2+) mobilization within single cells is graded in nature and dependent on both agonist concentration and receptor density...
  11. ncbi request reprint Lysophosphatidic acid-induced Ca2+ mobilization requires intracellular sphingosine 1-phosphate production. Potential involvement of endogenous EDG-4 receptors
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester, LE1 9HN United Kingdom
    J Biol Chem 275:38532-9. 2000
    ..Unlike traditional Ca(2+) release processes, this novel pathway does not require the progressive recruitment of elementary Ca(2+) events...
  12. doi request reprint A novel paradigm for rapid ABT-737-induced apoptosis involving outer mitochondrial membrane rupture in primary leukemia and lymphoma cells
    M Vogler
    MRC Toxicology Unit, University of Leicester, Leicester, UK
    Cell Death Differ 15:820-30. 2008
    ..This mechanism may be far more prevalent than hitherto recognized due to the failure of most methods, used to measure apoptosis, to recognize such a mechanism...
  13. pmc N-methyl-D-aspartate receptors mediate the phosphorylation and desensitization of muscarinic receptors in cerebellar granule neurons
    Adrian J Butcher
    Department of Cell Physiology and Pharmacology, University of Leicesterm, Hodgkin Building, Lancaster Road, Leicester LE1 9HN, United Kingdom
    J Biol Chem 284:17147-56. 2009
    ....
  14. ncbi request reprint Sphingosine 1-phosphate: a Ca2+ release mediator in the balance
    K W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, LE1 9HN, Leicester, UK
    Cell Calcium 32:335-41. 2002
    ..However, neither the mechanism underlying S1P generation, nor its actions at the endoplasmic reticulum are clear. Thus, the role of S1P as an intracellular mediator of Ca(2+) release remains in the balance...
  15. ncbi request reprint Single cell analysis and temporal profiling of agonist-mediated inositol 1,4,5-trisphosphate, Ca2+, diacylglycerol, and protein kinase C signaling using fluorescent biosensors
    Paula J Bartlett
    Department of Cell Physiology and Pharmacology, University of Leicester, Maurice Shock Medical Sciences Building, University Road, Leicester LE1 9HN, United Kingdom
    J Biol Chem 280:21837-46. 2005
    ..By acting on various components of the PLC signaling pathway the frequency-encoded Ca(2+) response is able to maintain signal specificity at a level downstream of PKC activation...
  16. ncbi request reprint Muscarinic acetylcholine receptor activation enhances hippocampal neuron excitability and potentiates synaptically evoked Ca(2+) signals via phosphatidylinositol 4,5-bisphosphate depletion
    Kenneth W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester, LE1 9HN, UK
    Mol Cell Neurosci 30:48-57. 2005
    ..These studies suggest that, in addition to producing the second messengers IP(3) and diacylglycerol, mACh receptor activation may directly utilize PIP(2) hydrolysis to regulate neuronal excitability...
  17. ncbi request reprint Ca2+/calmodulin-dependent translocation of sphingosine kinase: role in plasma membrane relocation but not activation
    Kenneth W Young
    Department of Cell Physiology and Pharmacology, Medical Sciences Building, University of Leicester, University Road, Leicester LE1 9HN, UK
    Cell Calcium 33:119-28. 2003
    ..These data suggest a role for Ca(2+)/calmodulin in controlling the subcellular distribution but not the activity of SPHK1a...
  18. ncbi request reprint Mitochondrial Ca2+ signalling in hippocampal neurons
    Kenneth W Young
    MRC Toxicology Unit, University of Leicester, Hodgkin Building, Lancaster Road, Leicester LE1 9HN, UK
    Cell Calcium 43:296-306. 2008
    ..Spatial regulation of mitochondrial Ca2+ uptake was determined by the magnitude of the cytosolic Ca2+ rise in each cellular compartment...
  19. ncbi request reprint Visualizing phosphoinositide signalling in single neurons gets a green light
    Stefan R Nahorski
    Department of Cell Physiology and Pharmacology, University of Leicester, Maurice Shock Medical Sciences Building, University Road, LE1 9HN, Leicester, UK
    Trends Neurosci 26:444-52. 2003
    ..This article reviews these new approaches and how they have provided novel insights into mechanisms underlying spatio-temporal Ca(2+) signals and phospholipase C activation in neurons...
  20. ncbi request reprint Altered expression of G(q/11alpha) protein shapes mGlu1 and mGlu5 receptor-mediated single cell inositol 1,4,5-trisphosphate and Ca(2+) signaling
    Peter J Atkinson
    Department of Cell Physiology and Pharmacology, University of Leicester, Maurice Shock Medical Sciences Building, University Road, Leicester, LE1 9HN, UK
    Mol Pharmacol 69:174-84. 2006
    ..These experiments indicate that altering G(q/11)alpha expression levels differentially affects spatiotemporal aspects of IP(3) and Ca(2+) signaling mediated by the mGlu1 and mGlu5 receptors...
  21. ncbi request reprint Cleavage of the plasma membrane Na+/Ca2+ exchanger in excitotoxicity
    Daniele Bano
    MRC Toxicology Unit, University of Leicester, Hodgkin Building, Lancaster Road LE1 9HN, Leicester, United Kingdom
    Cell 120:275-85. 2005
    ..Thus, proteolytic inactivation of NCX-driven neuronal Ca2+ extrusion is responsible for the delayed excitotoxic Ca2+ deregulation and neuronal death...
  22. ncbi request reprint The plasma membrane Na+/Ca2+ exchanger is cleaved by distinct protease families in neuronal cell death
    D Bano
    MRC Toxicology Unit, University of Leicester, Hodgkin Building, Lancaster Road LE1 9HN, Leicester, UK
    Ann N Y Acad Sci 1099:451-5. 2007
    ..Here we show that the NCX can also be cleaved by caspases in neurons undergoing apoptosis, which suggests that cleavage of the main Ca2+ extrusion pathway is a lethal event in multiple forms of cell death...
  23. pmc Dendritic spine loss and neurodegeneration is rescued by Rab11 in models of Huntington's disease
    P Richards
    MRC Toxicology Unit, University of Leicester, Hodgkin Building, Lancaster Road, Leicester, UK
    Cell Death Differ 18:191-200. 2011
    ..This mechanism may contribute to the toxicity caused by protein misfolding found in a number of neurodegenerative diseases...
  24. pmc Targeting autophagy potentiates tyrosine kinase inhibitor-induced cell death in Philadelphia chromosome-positive cells, including primary CML stem cells
    Cristian Bellodi
    University of Leicester, United Kingdom
    J Clin Invest 119:1109-23. 2009
    ..Together, these findings suggest that autophagy inhibitors may enhance the therapeutic effects of TKIs in the treatment of CML...
  25. ncbi request reprint A major functional role for phosphodiesterase 4D5 in human airway smooth muscle cells
    Charlotte K Billington
    Division of Therapeutics and Molecular Medicine, University of Nottingham, Queens Medical Centre, Nottingham, UK
    Am J Respir Cell Mol Biol 38:1-7. 2008
    ..These data suggest that PDE4D5, despite being a minor component of the tissue PDE pool, is the key physiological regulator of beta(2)AR-induced cAMP turnover within human airway smooth muscle...