C Winstanley

Summary

Affiliation: University of Liverpool
Country: UK

Publications

  1. ncbi request reprint Detection of type III secretion system genes in animal isolates of Bordetella bronchiseptica
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, P O Box 147, Liverpool, UK
    Vet Microbiol 72:329-37. 2000
  2. ncbi request reprint Improved flagellin genotyping in the Burkholderia cepacia complex
    Craig Winstanley
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, UK
    FEMS Microbiol Lett 229:9-14. 2003
  3. ncbi request reprint Spot the difference: applications of subtractive hybridisation to the study of bacterial pathogens
    Craig Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, UK
    J Med Microbiol 51:459-67. 2002
  4. ncbi request reprint Flagellin gene PCR-RFLP analysis of a panel of strains from the Burkholderia cepacia complex
    C Winstanley
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool
    J Med Microbiol 50:728-31. 2001
  5. ncbi request reprint Variation in Bordetella bronchiseptica flaA does not correlate with typing by macro-restriction analysis by pulsed-field gel electrophoresis
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool
    J Med Microbiol 50:255-60. 2001
  6. ncbi request reprint Type III secretion systems and pathogenicity islands
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool
    J Med Microbiol 50:116-26. 2001
  7. pmc Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom
    J Clin Microbiol 38:883-5. 2000
  8. ncbi request reprint Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster
    C Winstanley
    Department of Biomedical Sciences, University of Bradford, West Yorkshire
    J Med Microbiol 48:649-56. 1999
  9. ncbi request reprint Analysis of fliC variation among clinical isolates of Burkholderia cepacia
    C Winstanley
    Department of Biomedical Sciences, University of Bradford, West Yorkshire
    J Med Microbiol 48:657-62. 1999
  10. pmc Variation in flagellin genes and proteins of Burkholderia cepacia
    B A Hales
    Department of Biomedical Sciences, University of Bradford, West Yorkshire, United Kingdom
    J Bacteriol 180:1110-8. 1998

Collaborators

Detail Information

Publications48

  1. ncbi request reprint Detection of type III secretion system genes in animal isolates of Bordetella bronchiseptica
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, P O Box 147, Liverpool, UK
    Vet Microbiol 72:329-37. 2000
    ..bronchiseptica, it is likely that different clinical manifestations may be due to variations in gene expression or host factors, rather than the absence or presence of TTS genes...
  2. ncbi request reprint Improved flagellin genotyping in the Burkholderia cepacia complex
    Craig Winstanley
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, UK
    FEMS Microbiol Lett 229:9-14. 2003
    ..Although B. multivorans strains with larger fliC genes clustered together, relationships between strains based on fliC sequences did not generally correlate with genomovar status...
  3. ncbi request reprint Spot the difference: applications of subtractive hybridisation to the study of bacterial pathogens
    Craig Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, UK
    J Med Microbiol 51:459-67. 2002
    ..The application of these techniques has led to the identification of PIs, mobile genetic elements and variations in virulence gene expression in a range of bacterial pathogens...
  4. ncbi request reprint Flagellin gene PCR-RFLP analysis of a panel of strains from the Burkholderia cepacia complex
    C Winstanley
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool
    J Med Microbiol 50:728-31. 2001
    ..The level of variation suggests that flagellin genotyping is a useful method for discriminating between B. cepacia strains...
  5. ncbi request reprint Variation in Bordetella bronchiseptica flaA does not correlate with typing by macro-restriction analysis by pulsed-field gel electrophoresis
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool
    J Med Microbiol 50:255-60. 2001
    ..If so, relationships inferred on the basis of PFGE or other molecular methods for whole genome comparison should be treated with caution...
  6. ncbi request reprint Type III secretion systems and pathogenicity islands
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool
    J Med Microbiol 50:116-26. 2001
    ..This review discusses the current knowledge of the role of TTS PIs in pathogenicity, the genetic organisation and evolution of such systems,and the potential for using TTS systems as targets for novel treatments...
  7. pmc Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara(-) phenotypes
    C Winstanley
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom
    J Clin Microbiol 38:883-5. 2000
    ..The link between TTS genes and an Ara(-) phenotype suggests a role for TTS in virulence...
  8. ncbi request reprint Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster
    C Winstanley
    Department of Biomedical Sciences, University of Bradford, West Yorkshire
    J Med Microbiol 48:649-56. 1999
    ..solanacearum proteins. The presence of type III secretion-associated genes in B. pseudomallei pathogens suggests a possible role for type III secretion systems in the pathogenicity of this organism...
  9. ncbi request reprint Analysis of fliC variation among clinical isolates of Burkholderia cepacia
    C Winstanley
    Department of Biomedical Sciences, University of Bradford, West Yorkshire
    J Med Microbiol 48:657-62. 1999
    ..The variation in fliC sequences of B. cepacia was far greater than that with B. pseudomallei, supporting the view that 'B. cepacia', as currently defined, may comprise several different genomic species...
  10. pmc Variation in flagellin genes and proteins of Burkholderia cepacia
    B A Hales
    Department of Biomedical Sciences, University of Bradford, West Yorkshire, United Kingdom
    J Bacteriol 180:1110-8. 1998
    ....
  11. ncbi request reprint Flagellin gene variation between clinical and environmental isolates of Burkholderia pseudomallei contrasts with the invariance among clinical isolates
    C Winstanley
    Department of Biomedical Sciences, University of Bradford, West Yorkshire
    J Med Microbiol 47:689-94. 1998
    ..pseudomallei strains...
  12. ncbi request reprint Use of temporal temperature gradient gel electrophoresis to identify flaA and fim3 sequence types in Bordetella bronchiseptica
    A Shina
    Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, UK
    Lett Appl Microbiol 32:384-7. 2001
    ..bronchiseptica. Significance and Impact of the Study: PCR amplification coupled to TTGE offers a general method for the rapid screening of large numbers of microbial strains for variations in gene sequences...
  13. ncbi request reprint A putative type III secretion gene cluster is widely distributed in the Burkholderia cepacia complex but absent from genomovar I
    Y N Parsons
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, UK
    FEMS Microbiol Lett 203:103-8. 2001
    ..cepacia complex, including genomovar III. In contrast to TTS gene clusters in other bacteria, a putative gene homologous to the virB1 gene of Brucella suis was located directly downstream of bcscQR...
  14. ncbi request reprint Molecular cloning of two Pseudomonas flagellin genes and basal body structural genes
    C Winstanley
    Department of Genetics and Microbiology, University of Liverpool, UK
    Microbiology 140:2019-31. 1994
    ..The deduced molecular mass of P. putida PaW8 flagellin (68 kDa) differed significantly from its apparent molecular mass estimated by PAGE, possibly as a consequence of post-translational modification.(ABSTRACT TRUNCATED AT 250 WORDS)..
  15. pmc Comparison of Arcobacter isolation methods, and diversity of Arcobacter spp. in Cheshire, United Kingdom
    J Y Merga
    Institute of Infection and Global Health, National Centre for Zoonosis Research, University of Liverpool, Chester High Road, Neston CH64 7TE, United Kingdom
    Appl Environ Microbiol 77:1646-50. 2011
    ..from animal feces and demonstrated that the freezing of fecal samples prior to isolation reduces arcobacter recovery. MLST analysis of the isolates revealed a high level of diversity...
  16. ncbi request reprint Environmental contamination with an epidemic strain of Pseudomonas aeruginosa in a Liverpool cystic fibrosis centre, and study of its survival on dry surfaces
    S Panagea
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, Liverpool L69 3GA, UK
    J Hosp Infect 59:102-7. 2005
    ..Our study suggests that airborne dissemination plays a significant role in patient-to-patient spread of LES, and confirms the need to segregate those patients colonized by epidemic P. aeruginosa strains from all other CF patients...
  17. ncbi request reprint Molecular characterization and distribution of virulence-associated genes amongst Aeromonas isolates from Libya
    A I Abdullah
    Department Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, UK
    J Appl Microbiol 95:1001-7. 2003
    ..The aim of the study was to type 52 Aeromonas spp. isolates from chicken carcasses, children with diarrhoea and a hospital environment in Libya, and to determine the distribution of putative virulence genes amongst them...
  18. ncbi request reprint Characterization of a spirochaete isolated from a case of bovine digital dermatitis
    I Demirkan
    Department of Medical Microbiology, University of Liverpool, Liverpool, UK
    J Appl Microbiol 101:948-55. 2006
    ..The aim of the study was to characterize a spirochaete isolated from the lesions of a cow with digital dermatitis (DD)...
  19. pmc Isolation of a novel Campylobacter jejuni clone associated with the bank vole, Myodes glareolus
    N J Williams
    National Centre for Zoonosis Research, University of Liverpool, Leahurst, Neston, Cheshire CH64 7TE, United Kingdom
    Appl Environ Microbiol 76:7318-21. 2010
    ..jejuni and specific hosts. Here, we describe the isolation of a novel clone (sequence type 3704 [ST-3704]) of C. jejuni associated with the bank vole (Myodes glareolus)...
  20. pmc Increased morbidity associated with chronic infection by an epidemic Pseudomonas aeruginosa strain in CF patients
    M Al-Aloul
    Regional Adult Cystic Fibrosis Unit, The Cardiothoracic Centre, Liverpool, UK
    Thorax 59:334-6. 2004
    ..However, whether such infection carries a worse prognosis is unknown. To address this, the clinical course of a group of CF patients chronically infected by LES was compared with that in patients harbouring unique strains...
  21. doi request reprint A subset of mucosa-associated Escherichia coli isolates from patients with colon cancer, but not Crohn's disease, share pathogenicity islands with urinary pathogenic E. coli
    Christina Bronowski
    Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Liverpool, UK
    Microbiology 154:571-83. 2008
    ..This enabled us to identify a group of isolates from colon cancer (30-40 %) carrying multiple genes previously categorized as UPEC-specific and implicated in virulence...
  22. pmc Isolation of Waddlia malaysiensis, a novel intracellular bacterium, from fruit bat (Eonycteris spelaea)
    Paul K B Chua
    University of Liverpool, Liverpool, L69 3GA, UK
    Emerg Infect Dis 11:271-7. 2005
    ..The 16S and 23S rRNA gene signatures were only 91% identical. We propose this novel bacterium be called W. malaysiensis...
  23. pmc A cystic fibrosis epidemic strain of Pseudomonas aeruginosa displays enhanced virulence and antimicrobial resistance
    Prabhakar Salunkhe
    Clinical Research Group OE 6711, Medical Research School Hannover, Germany
    J Bacteriol 187:4908-20. 2005
    ....
  24. ncbi request reprint Identical Burkholderia cepacia complex strain types isolated from multiple patients attending a hospital in Brazil
    Wail Assaad
    J Med Microbiol 55:247-9. 2006
  25. pmc Use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of Burkholderia pseudomallei
    Kwanjit Duangsonk
    Division of Medical Microbiology, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, United Kingdom
    J Clin Microbiol 44:1323-34. 2006
    ....
  26. ncbi request reprint Diagnostic multiplex PCR assay for the identification of the Liverpool, Midlands 1 and Manchester CF epidemic strains of Pseudomonas aeruginosa
    Joanne L Fothergill
    Division of Medical Microbiology, University of Liverpool, Daulby Street, Liverpool L69 3GA, United Kingdom
    J Cyst Fibros 7:258-61. 2008
    ..The test was 100% sensitive and 100% specific in the identification of these UK CF epidemic strains...
  27. pmc Identification, typing, and insecticidal activity of Xenorhabdus isolates from entomopathogenic nematodes in United Kingdom soil and characterization of the xpt toxin loci
    Martin Sergeant
    Warwick HRI, University of Warwick, Warwick CV35 9EF, United Kingdom
    Appl Environ Microbiol 72:5895-907. 2006
    ..bovienii strain I73 on a type 2 transposon structure and by the variable patterns of insecticidal activity in X. bovienii isolates, even among closely related strains...
  28. ncbi request reprint Use of suppression subtractive hybridization to examine the accessory genome of the Liverpool cystic fibrosis epidemic strain of Pseudomonas aeruginosa
    Catherine H M Smart
    Division of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool L69 3GA, UK
    J Med Microbiol 55:677-88. 2006
    ..Rare false positives were found with the PS21 test. The authors suggest that a second PCR assay designed to detect SSH sequence LESF9 can be used to confirm the identity of the most prevalent CF epidemic lineage in the UK...
  29. pmc Use of suppression subtractive hybridisation to extend our knowledge of genome diversity in Campylobacter jejuni
    Philip J Hepworth
    Division of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, UK
    BMC Genomics 8:110. 2007
    ..jejuni isolates from different sources (rabbit, cattle, wild bird) were carried out, designed to assess genomic variability within and between common multilocus sequence type (MLST) clonal complexes (ST-21, ST-42, ST-45 and ST-61)...
  30. ncbi request reprint Sequence divergence in type III secretion gene clusters of the Burkholderia cepacia complex
    Kerry J Glendinning
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Liverpool L69 3GA, UK
    FEMS Microbiol Lett 235:229-35. 2004
    ..Nucleotide sequence data obtained for the equivalent region of the B. cepacia genome indicate the absence of TTS structural genes due to a rearrangement likely to involve more than one step...
  31. ncbi request reprint Distribution of type III secretion gene clusters in Burkholderia pseudomallei, B. thailandensis and B. mallei
    Lucille Rainbow
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, UK
    J Med Microbiol 51:374-84. 2002
    ..PCR assays indicated that TTS2 was also present in B. thailandensis. TTS1 and TTS2 are similar in gene organisation, but nucleotide sequences are sufficiently divergent to suggest that the two TTS systems may have different roles...
  32. ncbi request reprint Persistent and aggressive bacteria in the lungs of cystic fibrosis children
    C Anthony Hart
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, UK
    Br Med Bull 61:81-96. 2002
    ..A greater understanding of how the bacteria evade the host defences and produce infection is needed...
  33. ncbi request reprint Distribution of fim3 and flaA TTGE sequence types amongst isolates of Bordetella bronchiseptica from different host animals
    Angela Shina
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool
    J Med Microbiol 51:557-63. 2002
    ..As both fimbriae and flagella have been implicated in adherence of bordetellae to host cells, sequence variations in fimbrial proteins and FlaA may have a role to play in host preferences...
  34. pmc Use of subtractive hybridization to identify a diagnostic probe for a cystic fibrosis epidemic strain of Pseudomonas aeruginosa
    Yasmin N Parsons
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom
    J Clin Microbiol 40:4607-11. 2002
    ..A sequence present in all isolates of the Liverpool epidemic strain was used to develop a diagnostic PCR test for identification of the strain from colonies or directly from sputum samples...
  35. ncbi request reprint Suppression-subtractive hybridisation reveals variations in gene distribution amongst the Burkholderia cepacia complex, including the presence in some strains of a genomic island containing putative polysaccharide production genes
    Yasmin N Parsons
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Daulby Street, UK
    Arch Microbiol 179:214-23. 2003
    ..mallei wcbO that is lacking in other ET12 isolates. Genes from this cluster are expressed during exponential growth in broth...
  36. ncbi request reprint Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis
    Craig Winstanley
    Division of Medical Microbiology and Genitourinary Medicine, School of Clinical Laboratory Sciences, University of Liverpool, Liverpool L69 3GA, UK
    J Med Microbiol 54:519-26. 2005
    ..There was a correlation between the presence of exoS and twitching activity (P = 0.04), suggesting that a combination of exoS genotype and good twitching activity may have a role to play in ExoU-independent corneal virulence...
  37. ncbi request reprint Type III secretion homologs are present in Brucella melitensis, B. ovis, and B. suis biovars 1, 2, and 3
    Abdullah I Abdallah
    Department of Medical Microbiology and Genito Urinary Medicine, University of Liverpool, Duncan Building, Daulby Street, P O Box 147, Liverpool L69 3GA, UK
    Curr Microbiol 46:241-5. 2003
    ....
  38. pmc Molecular typing of, and distribution of genetic markers among, Burkholderia cepacia complex isolates from Brazil
    Maria G Detsika
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom
    J Clin Microbiol 41:4148-53. 2003
    ..Nine genomovar IIIA isolates from different non-CF patients and clinical sources had identical genotypes, indicating the presence of a common clone...
  39. ncbi request reprint Identification and expression of a Burkholderia pseudomallei collagenase in Escherichia coli
    Lucille Rainbow
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, United Kingdom
    Curr Microbiol 48:300-4. 2004
    ..The putative collagenase gene was cloned and expressed as a fusion protein in Escherichia coli. Cell lysates from Escherichia coli containing the recombinant protein exhibited detectable gelatinase and collagenase activities...
  40. ncbi request reprint Conservation of the opcL gene encoding the peptidoglycan-associated outer-membrane lipoprotein among representatives of the Burkholderia cepacia complex
    Maria Plesa
    Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute of Biotechnology, Vrije Universiteit Brussel, Building E, Room 6 2, Pleinlaan 2, B 1050 Brussels, Belgium
    J Med Microbiol 53:389-98. 2004
    ..cepacia; sequence data derived from the amplicons identified the colonizing strains as B. cenocepacia (genomovar III, n = 14) and B. multivorans (n = 1)...
  41. ncbi request reprint PCR-based detection of a cystic fibrosis epidemic strain of Pseudomonas Aeruginosa
    Stavroula Panagea
    Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, UK
    Mol Diagn 7:195-200. 2003
    ..This study evaluates a new diagnostic PCR assay based on a unique DNA sequence (PS21) of LES, for its identification of colonies directly from sputum...
  42. ncbi request reprint Development of a diagnostic test for the Midlands 1 cystic fibrosis epidemic strain of Pseudomonas aeruginosa
    Catherine H M Smart
    Division of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, UK
    J Med Microbiol 55:1085-91. 2006
    ..The identity was confirmed using typing by PFGE. The PCR test should facilitate a greater understanding of the distribution of the Midlands 1 strain in the UK and elsewhere...
  43. ncbi request reprint Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa
    Magaly de Chial
    Flanders Interuniversity Institute of Biotechnology VIB6, Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6 6, Pleinlaan 2, B 1050 Brussels, Belgium
    Microbiology 149:821-31. 2003
    ..All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAI has a TAT export sequence at its N-terminal end...
  44. pmc In vivo growth of Pseudomonas aeruginosa strains PAO1 and PA14 and the hypervirulent strain LESB58 in a rat model of chronic lung infection
    Irena Kukavica-Ibrulj
    Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Pavillon Charles Eugène Marchand, Biologie Médicale, Faculte de Medecine, Universite Laval, Laval, Quebec G1K 7P4, Canada
    J Bacteriol 190:2804-13. 2008
    ..These differences were further confirmed with biofilm and motility in vitro assays, where strain LESB58 produced more biofilm but had less capacity for motility than PAO1 and PA14...
  45. ncbi request reprint Characterization of epithelial IL-8 response to inflammatory bowel disease mucosal E. coli and its inhibition by mesalamine
    Sreedhar Subramanian
    Division of Gastroenterology, University School of Clinical Science, Liverpool, UK
    Inflamm Bowel Dis 14:162-75. 2008
    ..Mucosally adherent E. coli are found in inflammatory bowel disease (IBD) and colon cancer. They promote release of the proinflammatory cytokine interleukin-8 (IL-8). We explored mechanisms for this release and its inhibition by drugs...
  46. ncbi request reprint Detection and quantification of minority HIV isolates harbouring the D30N mutation by real-time PCR amplification
    Maria G Detsika
    Department of Pharmacology and Therapeutics, University of Liverpool, 70 Pembroke Place, Liverpool L69 3GF, UK
    J Antimicrob Chemother 60:881-4. 2007
    ..Genotypic resistance is currently assessed by direct sequencing and cannot detect resistant species below 20%. Real-time PCR amplification was assessed for its ability to detect the signature mutation for nelfinavir, D30N...
  47. pmc Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain
    Joanne L Fothergill
    Division of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, UK
    BMC Microbiol 7:45. 2007
    ..Our aim was to determine the prevalence of this unusual phenotype amongst isolates of the epidemic strain, and to study other intraclonal phenotypic and genotypic variations...
  48. ncbi request reprint Caenorhabditis elegans killing assay as an infection model to study the role of type III secretion in Burkholderia cenocepacia
    Kevin M Markey
    J Med Microbiol 55:967-9. 2006