R L Williams

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi request reprint Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus
    Bojana Panic
    MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 12:863-74. 2003
  2. ncbi request reprint The emerging shape of the ESCRT machinery
    Roger L Williams
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Rev Mol Cell Biol 8:355-68. 2007
  3. pmc Structure of lipid kinase p110β/p85β elucidates an unusual SH2-domain-mediated inhibitory mechanism
    Xuxiao Zhang
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Mol Cell 41:567-78. 2011
  4. pmc Dynamic steps in receptor tyrosine kinase mediated activation of class IA phosphoinositide 3-kinases (PI3K) captured by H/D exchange (HDX-MS)
    John E Burke
    Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Adv Biol Regul 53:97-110. 2013
  5. pmc The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain
    Monica Agromayor
    Department of Infectious Diseases, King s College London School of Medicine, London SE1 9RT, UK
    Structure 20:414-28. 2012
  6. doi request reprint Form and flexibility in phosphoinositide 3-kinases
    Roger Williams
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Biochem Soc Trans 37:615-26. 2009
  7. ncbi request reprint Mammalian phosphoinositide-specific phospholipase C
    R L Williams
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    Biochim Biophys Acta 1441:255-67. 1999
  8. ncbi request reprint The structure of a tunicate C-type lectin from Polyandrocarpa misakiensis complexed with D -galactose
    S F Poget
    Cambridge Centre for Protein Engineering, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    J Mol Biol 290:867-79. 1999
  9. ncbi request reprint Structural insights into phosphoinositide 3-kinase catalysis and signalling
    E H Walker
    MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK
    Nature 402:313-20. 1999
  10. ncbi request reprint The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate
    J Bravo
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 8:829-39. 2001

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus
    Bojana Panic
    MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 12:863-74. 2003
    ..Despite no conservation of sequence, topology, or even helical direction, several other effectors form similar interactions with small GTPases via a pair of alpha helices, suggesting a common structural basis for effector recognition...
  2. ncbi request reprint The emerging shape of the ESCRT machinery
    Roger L Williams
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Rev Mol Cell Biol 8:355-68. 2007
    ....
  3. pmc Structure of lipid kinase p110β/p85β elucidates an unusual SH2-domain-mediated inhibitory mechanism
    Xuxiao Zhang
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Mol Cell 41:567-78. 2011
    ..This contrasts with the nSH2 where the pY-binding site itself forms the inhibitory contact. This establishes an unusual mechanism by which p85 SH2 domains contribute to RTK signaling specificities...
  4. pmc Dynamic steps in receptor tyrosine kinase mediated activation of class IA phosphoinositide 3-kinases (PI3K) captured by H/D exchange (HDX-MS)
    John E Burke
    Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Adv Biol Regul 53:97-110. 2013
    ..The unique dynamic relationships of the different p110 isozymes to the p85 subunit may facilitate new strategies for specific inhibitors of the PI3Ks...
  5. pmc The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain
    Monica Agromayor
    Department of Infectious Diseases, King s College London School of Medicine, London SE1 9RT, UK
    Structure 20:414-28. 2012
    ....
  6. doi request reprint Form and flexibility in phosphoinositide 3-kinases
    Roger Williams
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Biochem Soc Trans 37:615-26. 2009
    ..Structural studies of the class I PI3Ks suggest that flexibility may be a component of the catalytic cycle of the enzymes...
  7. ncbi request reprint Mammalian phosphoinositide-specific phospholipase C
    R L Williams
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    Biochim Biophys Acta 1441:255-67. 1999
    ..The roles of the PH, EF hand, C2, SH2 and SH3 modules in regulation of these enzymes and in interactions with membranes and other proteins is becoming apparent from recent structural and functional studies...
  8. ncbi request reprint The structure of a tunicate C-type lectin from Polyandrocarpa misakiensis complexed with D -galactose
    S F Poget
    Cambridge Centre for Protein Engineering, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK
    J Mol Biol 290:867-79. 1999
    ..Possible biological functions can more easily be understood from the fact that TC14 is a dimer under physiological conditions...
  9. ncbi request reprint Structural insights into phosphoinositide 3-kinase catalysis and signalling
    E H Walker
    MRC Laboratory of Molecular Biology, MRC Centre, Cambridge, UK
    Nature 402:313-20. 1999
    ....
  10. ncbi request reprint The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate
    J Bravo
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 8:829-39. 2001
    ..The SH3 domain present in the full-length protein does not affect soluble PtdIns(3)P binding to the p40(phox) PX domain...
  11. ncbi request reprint Structural views of phosphoinositide-specific phospholipase C: signalling the way ahead
    R L Williams
    Centre for Protein Engineering, MRC Centre, Cambridge, UK
    Structure 4:1387-94. 1996
    ....
  12. ncbi request reprint Crystal structure of a calcium-phospholipid binding domain from cytosolic phospholipase A2
    O Perisic
    Medical Research Council Laboratory of Molecular Biology, Medical Research Council Centre, Cambridge, United Kingdom
    J Biol Chem 273:1596-604. 1998
    ..Although hydrophobic interactions predominate for cPLA2, for other C2 domains such as in "conventional" protein kinase C and synaptotagmins, electrostatic forces prevail...
  13. ncbi request reprint Crystal structure of the two N-terminal domains of g3p from filamentous phage fd at 1.9 A: evidence for conformational lability
    P Holliger
    MRC Centre for Protein Engineering, Cambridge CB2 2QH, UK
    J Mol Biol 288:649-57. 1999
    ....
  14. ncbi request reprint Calcium-dependent membrane penetration is a hallmark of the C2 domain of cytosolic phospholipase A2 whereas the C2A domain of synaptotagmin binds membranes electrostatically
    B Davletov
    Medical Research Council, Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge CB22QH, United Kingdom
    J Biol Chem 273:19093-6. 1998
    ..Because soluble phospholipid head group analogues do not compete with binding of either SytIC2A or cPLA2C2 to vesicles, it is likely that membrane binding by these domains involves multiple interactions...
  15. pmc Structural basis for the heterodimeric interaction between the acute leukaemia-associated transcription factors AML1 and CBFbeta
    A J Warren
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 19:3004-15. 2000
    ....
  16. ncbi request reprint Crystal structure of a mammalian phosphoinositide-specific phospholipase C delta
    L O Essen
    Center for Protein Engineering, MRC Centre, Cambridge, UK
    Nature 380:595-602. 1996
    ..The regulation and reversible membrane association of PI-PLC may serve as a model for understanding other multidomain enzymes involved in phospholipid signalling...
  17. pmc Regulation of lipid binding underlies the activation mechanism of class IA PI3-kinases
    W C Hon
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Oncogene 31:3655-66. 2012
    ....
  18. ncbi request reprint The crystal structure of a human nucleoside diphosphate kinase, NM23-H2
    P A Webb
    Centre for Protein Engineering, MRC Centre, Cambridge, UK
    J Mol Biol 251:574-87. 1995
    ..In contrast, Ser120 is buried, and is most likely phosphorylated by a direct transfer from the phosphohistidine intermediate of the reaction mechanism...
  19. ncbi request reprint ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes
    Hsiangling Teo
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Dev Cell 7:559-69. 2004
    ..Vps25 is the chief subunit responsible for Vps20 recruitment. This interaction dramatically increases binding of both components to lipid vesicles in vitro...
  20. pmc Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction
    Dimitrios Karathanassis
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 21:5057-68. 2002
    ....
  21. ncbi request reprint PB1 domain-mediated heterodimerization in NADPH oxidase and signaling complexes of atypical protein kinase C with Par6 and p62
    Michael I Wilson
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 12:39-50. 2003
    ..Both Par6 and p62 use their basic "back" to interact with the OPCA motif on the "front" of the PKC zeta. Besides heterodimeric interactions, some PB1 domains, like the p62 PB1, can make homotypic front-to-back arrays...
  22. ncbi request reprint Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4
    Takayuki Obita
    MRC Laboratory of Molecular Biology, Medical Research Council Centre, Cambridge CB2 0QH, UK
    Nature 449:735-9. 2007
    ....
  23. pmc The structural basis of novel endosome anchoring activity of KIF16B kinesin
    Nichole R Blatner
    Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA
    EMBO J 26:3709-19. 2007
    ....
  24. ncbi request reprint Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit
    Nabil Miled
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 317:239-42. 2007
    ..These studies extend our understanding of the architecture of PI3Ks and provide insight into how two classes of mutations that cause a gain in function can lead to cancer...
  25. ncbi request reprint Structural and membrane binding analysis of the Phox homology domain of Bem1p: basis of phosphatidylinositol 4-phosphate specificity
    Robert V Stahelin
    Department of Chemistry, University of Illinois, Chicago, Illinois 60607 7061, USA
    J Biol Chem 282:25737-47. 2007
    ..This structural and functional study of the PtdIns(4)P-binding Bem1p PX domain provides new insight into the diverse PI specificities and membrane-binding mechanisms of PX domains...
  26. pmc Tumour prevention by a single antibody domain targeting the interaction of signal transduction proteins with RAS
    Tomoyuki Tanaka
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK
    EMBO J 26:3250-9. 2007
    ..In addition, this method illustrates a general means for interfering with protein interactions that are commonly considered intractable as conventional drug targets...
  27. ncbi request reprint Structural insights into endosomal sorting complex required for transport (ESCRT-I) recognition of ubiquitinated proteins
    Hsiangling Teo
    Medical Research Council Laboratory of Molecular Biology and Centre for Protein Engineering, Medical Research Council Centre, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 279:28689-96. 2004
    ..The structure provides a detailed framework for design of mutants that can specifically affect ESCRT-I-dependent sorting of ubiquitinated cargo without affecting Vps27-mediated delivery of cargo to endosomes...
  28. ncbi request reprint Structure of a human inositol 1,4,5-trisphosphate 3-kinase: substrate binding reveals why it is not a phosphoinositide 3-kinase
    Beatriz Gonzalez
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 15:689-701. 2004
    ..This lobe embraces all of the phosphates of Ins(1,4,5)P3 in a positively charged pocket, explaining the enzyme's substrate specificity and its inability to phosphorylate PtdIns(4,5)P2, the membrane-resident analog of Ins(1,4,5)P3...
  29. pmc A segment of cold shock protein directs the folding of a combinatorial protein
    Stephanie de Bono
    Centre for Protein Engineering and Laboratory of Molecular Biology, Medical Research Council Centre, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 102:1396-401. 2005
    ....
  30. ncbi request reprint ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes
    Hsiangling Teo
    MRC Laboratory of Molecular Biology, Medical Research Council Centre, Cambridge, CB2 2QH, United Kingdom
    Cell 125:99-111. 2006
    ..The simultaneous and reinforcing interactions of ESCRT-II GLUE domain with membranes, ESCRT-I, and ubiquitin are critical for ubiquitinated cargo progression from early to late endosomes...
  31. pmc Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication
    Sam Li
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England, UK
    J Cell Biol 173:867-77. 2006
    ..This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly...
  32. ncbi request reprint Structural and membrane binding analysis of the Phox homology domain of phosphoinositide 3-kinase-C2alpha
    Robert V Stahelin
    Departments of Chemistry and Medicinal Chemistry and Pharmacognosy, University of Illinois, Chicago, Illinois 60607, USA
    J Biol Chem 281:39396-406. 2006
    ....
  33. pmc Structural insight into the ESCRT-I/-II link and its role in MVB trafficking
    David J Gill
    MRC Laboratory of Molecular Biology, Medical Research Council Centre, Cambridge, UK
    EMBO J 26:600-12. 2007
    ..Mvb12 does not affect the affinity of ESCRT-I for ESCRT-II in vitro. Our data suggest a complex regulatory mechanism for the ESCRT-I/-II link in yeast...
  34. pmc A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling
    Zachary A Knight
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Cell 125:733-47. 2006
    ..These results illustrate systematic target validation using a matrix of inhibitors that span a protein family...
  35. ncbi request reprint Structural basis for the interaction of [E160A-E189A]-trichosanthin with adenine
    Pang Chui Shaw
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, People s Republic of China
    Toxicon 41:575-81. 2003
    ....
  36. ncbi request reprint The role of phosphoinositides and phosphorylation in regulation of NADPH oxidase
    Olga Perisic
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Adv Enzyme Regul 44:279-98. 2004