Robert J White

Summary

Affiliation: University of Glasgow
Country: UK

Publications

  1. ncbi request reprint RNA polymerases I and III, growth control and cancer
    Robert J White
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, G12 8QQ, UK
    Nat Rev Mol Cell Biol 6:69-78. 2005
  2. pmc mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, and targets their repressor Maf1
    Theodoros Kantidakis
    Beatson Institute for Cancer Research, Glasgow G61 1BD, United Kingdom
    Proc Natl Acad Sci U S A 107:11823-8. 2010
  3. pmc Dr1 (NC2) is present at tRNA genes and represses their transcription in human cells
    Theodoros Kantidakis
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK
    Nucleic Acids Res 38:1228-39. 2010
  4. pmc A test of the model that RNA polymerase III transcription is regulated by selective induction of the 110 kDa subunit of TFIIIC
    Fiona Innes
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, UK
    Nucleic Acids Res 34:3399-407. 2006
  5. pmc RNA polymerase III transcription is repressed in response to the tumour suppressor ARF
    Jennifer P Morton
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, UK
    Nucleic Acids Res 35:3046-52. 2007
  6. doi request reprint Transcription by RNA polymerase III: more complex than we thought
    Robert J White
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Nat Rev Genet 12:459-63. 2011
  7. pmc The mitogen-activated protein (MAP) kinase ERK induces tRNA synthesis by phosphorylating TFIIIB
    Zoe A Felton-Edkins
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, UK
    EMBO J 22:2422-32. 2003
  8. pmc Regulation of RNA polymerase III transcription during hypertrophic growth
    Sarah J Goodfellow
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    EMBO J 25:1522-33. 2006
  9. pmc p53 represses RNA polymerase III transcription by targeting TBP and inhibiting promoter occupancy by TFIIIB
    Diane Crighton
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK
    EMBO J 22:2810-20. 2003
  10. doi request reprint Elevated tRNA(iMet) synthesis can drive cell proliferation and oncogenic transformation
    Lynne Marshall
    Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Cell 133:78-89. 2008

Collaborators

Detail Information

Publications32

  1. ncbi request reprint RNA polymerases I and III, growth control and cancer
    Robert J White
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, G12 8QQ, UK
    Nat Rev Mol Cell Biol 6:69-78. 2005
    ..Such events might have a significant impact on the growth potential of tumours...
  2. pmc mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, and targets their repressor Maf1
    Theodoros Kantidakis
    Beatson Institute for Cancer Research, Glasgow G61 1BD, United Kingdom
    Proc Natl Acad Sci U S A 107:11823-8. 2010
    ..We conclude that mTOR associates with TFIIIC, is recruited to pol III-transcribed genes, and relieves their repression by Maf1...
  3. pmc Dr1 (NC2) is present at tRNA genes and represses their transcription in human cells
    Theodoros Kantidakis
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK
    Nucleic Acids Res 38:1228-39. 2010
    ..On the basis of these data, we conclude that the physiological functions of human Dr1 include regulation of pol III transcription...
  4. pmc A test of the model that RNA polymerase III transcription is regulated by selective induction of the 110 kDa subunit of TFIIIC
    Fiona Innes
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, UK
    Nucleic Acids Res 34:3399-407. 2006
    ..However, little or no effect is seen on the expression of pol III transcripts. The data argue against the model that pol III transcription can be effectively modulated through the specific induction of TFIIIC110...
  5. pmc RNA polymerase III transcription is repressed in response to the tumour suppressor ARF
    Jennifer P Morton
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, UK
    Nucleic Acids Res 35:3046-52. 2007
    ..However, in contrast to its effects on rRNA synthesis, ARF is unable to inhibit tRNA gene transcription when p53 is ablated. These results add to the growing list of cellular changes that can be triggered by ARF induction...
  6. doi request reprint Transcription by RNA polymerase III: more complex than we thought
    Robert J White
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Nat Rev Genet 12:459-63. 2011
    ..Here I describe these novel insights and consider their implications and the questions that remain...
  7. pmc The mitogen-activated protein (MAP) kinase ERK induces tRNA synthesis by phosphorylating TFIIIB
    Zoe A Felton-Edkins
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, UK
    EMBO J 22:2422-32. 2003
    ..These data identify a novel target for ERK, and suggest that its ability to stimulate biosynthetic capacity and growth involves direct transcriptional activation of tRNA and 5S rRNA genes...
  8. pmc Regulation of RNA polymerase III transcription during hypertrophic growth
    Sarah J Goodfellow
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    EMBO J 25:1522-33. 2006
    ..Increased production of tRNA and 5S rRNA will contribute to the enhanced translational capacity required to sustain hypertrophic growth...
  9. pmc p53 represses RNA polymerase III transcription by targeting TBP and inhibiting promoter occupancy by TFIIIB
    Diane Crighton
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK
    EMBO J 22:2810-20. 2003
    ..Together our results support the idea that p53 represses RNA pol III transcription through direct interactions with TBP, preventing promoter occupancy by TFIIIB...
  10. doi request reprint Elevated tRNA(iMet) synthesis can drive cell proliferation and oncogenic transformation
    Lynne Marshall
    Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Cell 133:78-89. 2008
    ..Overexpression of tRNA(iMet) is sufficient to stimulate cell proliferation and allow immortalized fibroblasts to form foci in culture and tumors in mice. The data indicate that elevated tRNA synthesis can promote cellular transformation...
  11. doi request reprint Regulation of RNA polymerase III transcription by Maf1 in mammalian cells
    Sarah J Goodfellow
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    J Mol Biol 378:481-91. 2008
    ..These aspects of Maf1 function have been conserved between yeast and mammals and are therefore likely to be of fundamental importance in controlling pol III transcriptional activity...
  12. pmc TRRAP and GCN5 are used by c-Myc to activate RNA polymerase III transcription
    Niall S Kenneth
    Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Proc Natl Acad Sci U S A 104:14917-22. 2007
    ..The selective acetylation of histone H3 distinguishes pol III activation by c-Myc from mechanisms observed in other systems...
  13. pmc Hypoxic stress suppresses RNA polymerase III recruitment and tRNA gene transcription in cardiomyocytes
    Isabelle Ernens
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Nucleic Acids Res 34:286-94. 2006
    ..These effects are independent of cell cycle changes...
  14. pmc TFIIIB is phosphorylated, disrupted and selectively released from tRNA promoters during mitosis in vivo
    Jennifer A Fairley
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK
    EMBO J 22:5841-50. 2003
    ..Repression can be reversed by raising the concentration of Brf1 or Bdp1. The data support a model in which hyperphosphorylation disrupts TFIIIB during mitosis, compromising its ability to support transcription...
  15. pmc Regulation of TFIIIB during F9 cell differentiation
    Dimitris Athineos
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    BMC Mol Biol 11:21. 2010
    ..The goal of this study was to identify molecular changes that can account for the low activity of TFIIIB following F9 cell differentiation...
  16. ncbi request reprint Regulation of RNA polymerase III transcription during mammalian cell growth
    Sarah J Goodfellow
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Cell Cycle 6:2323-6. 2007
    ..The complex network of strategies that couple pol III transcription with cell growth suggest that stringent control of this system is of fundamental importance...
  17. doi request reprint Direct regulation of tRNA and 5S rRNA gene transcription by Polo-like kinase 1
    Jennifer A Fairley
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Mol Cell 45:541-52. 2012
    ..Genomic instability is apparent in cells with Brf1 T270 mutated to alanine to resist Plk1-directed inactivation, suggesting that chromosome segregation is vulnerable to inappropriate pol III activity...
  18. ncbi request reprint Epstein-Barr virus induces cellular transcription factors to allow active expression of EBER genes by RNA polymerase III
    Zoe A Felton-Edkins
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    J Biol Chem 281:33871-80. 2006
    ..The elevated levels of BDP1 and TFIIIC in EBV-positive cells stimulate production of tRNA, 7SL, and 5S rRNA. Abnormally high expression of these cellular pol III products may contribute to the ability of EBV to enhance growth potential...
  19. ncbi request reprint Direct activation of RNA polymerase III transcription by c-Myc
    Natividad Gomez-Roman
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, G12 8QQ, UK
    Nature 421:290-4. 2003
    ..These results suggest that activation of pol III may have a role in the ability of c-Myc to stimulate cell growth...
  20. pmc Human La is found at RNA polymerase III-transcribed genes in vivo
    Jennifer A Fairley
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Proc Natl Acad Sci U S A 102:18350-5. 2005
    ..However, gene occupancy depends on the phosphorylation status of La, with the less prevalent, unphosphorylated form being found selectively on pol III templates...
  21. ncbi request reprint Deregulation of RNA polymerase III transcription in cervical epithelium in response to high-risk human papillomavirus
    Nicole L Daly
    Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK
    Oncogene 24:880-8. 2005
    ..The resultant increase in biosynthetic capacity may contribute to deregulated cell growth...
  22. ncbi request reprint Several regions of p53 are involved in repression of RNA polymerase III transcription
    Torsten Stein
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK
    Oncogene 21:5540-7. 2002
    ..These observations reveal that repression of pol III transcription is a complex function involving multiple regions of p53 extending throughout much of the protein...
  23. doi request reprint Non-coding RNA production by RNA polymerase III is implicated in cancer
    Lynne Marshall
    Beatson Institute for Cancer Research, Bearsden, Glasgow, UK
    Nat Rev Cancer 8:911-4. 2008
    ..New evidence suggests that Pol III output can substantially affect transformation...
  24. pmc Recruitment of RNA polymerase III in vivo
    Niall S Kenneth
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, G12 8QQ, UK
    Nucleic Acids Res 36:3757-64. 2008
    ..Promoter occupancy by pol II is unaffected, despite sharing five subunits with the pol III core. These observations provide evidence for the validity in vivo of the model for pol III recruitment that was built on biochemical data...
  25. ncbi request reprint Direct regulation of RNA polymerase III transcription by RB, p53 and c-Myc
    Zoe A Felton-Edkins
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Cell Cycle 2:181-4. 2003
    ..Availability of pol III products may be an important determinant of a cell's capacity to grow. The ability to regulate pol III output may therefore be integral to the growth control functions of RB, p53 and c-Myc...
  26. pmc CK2 forms a stable complex with TFIIIB and activates RNA polymerase III transcription in human cells
    Imogen M Johnston
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Mol Cell Biol 22:3757-68. 2002
    ..CK2 provides a rare example of an endogenous activity that operates on the PolIII system in both mammals and yeasts. Such evolutionary conservation suggests that this control may be of fundamental importance...
  27. doi request reprint RNA polymerases I and III, non-coding RNAs and cancer
    Robert J White
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK
    Trends Genet 24:622-9. 2008
    ..The resultant increases in rRNA and tRNA expression might promote the generation of cancers...
  28. ncbi request reprint RNA polymerase III transcription and cancer
    Robert J White
    Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Davidson Building, Glasgow G12 8QQ, UK
    Oncogene 23:3208-16. 2004
    ..In addition to effects on protein synthesis and growth, there is a precedent for a pol III product having oncogenic activity...
  29. doi request reprint Coordinated control of the gene expression machinery
    Robert J White
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK
    Trends Genet 26:214-20. 2010
    ..This occurs at both the transcriptional level, through the coordinate regulation of RNA polymerases I-III and downstream in the coordinate regulation of transcription with RNA processing and translation...
  30. ncbi request reprint Regulation by c-Myc of ncRNA expression
    Niall S Kenneth
    Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Curr Opin Genet Dev 19:38-43. 2009
    ..This involves it regulating the transcriptional activity of three different RNA polymerases. These ncRNAs are likely to contribute substantially to the complex biology and pathology that is associated with c-Myc...
  31. ncbi request reprint RNA polymerase III transcription can be derepressed by oncogenes or mutations that compromise p53 function in tumours and Li-Fraumeni syndrome
    Torsten Stein
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    Oncogene 21:2961-70. 2002
    ..Our data suggest that p53 status can have a profound effect upon pol III transcription and hence on the biosynthetic capacity of cells...
  32. ncbi request reprint Multiple mechanisms contribute to the activation of RNA polymerase III transcription in cells transformed by papovaviruses
    Zoe A Felton-Edkins
    Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, United Kingdom
    J Biol Chem 277:48182-91. 2002
    ..Middle T can serve as a potent activator of a pol III reporter in transfected cells. Several distinct mechanisms therefore contribute to the high levels of pol III transcription that accompany transformation by Py and SV40...