Malcolm F White
Affiliation: University of St Andrews
Rouillon C, Athukoralage J, Graham S, Gruschow S, White M. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems. Methods Enzymol. 2019;616:191-218 pubmed publisher
..The methods described are based on our recent studies of the type III CRISPR system in Sulfolobus solfataricus, but are widely applicable to other type III systems. ..
Athukoralage J, Rouillon C, Graham S, Gruschow S, White M. Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate. Nature. 2018;562:277-280 pubmed publisher
..The identification of ring nucleases adds an important insight to the CRISPR system. ..
Constantinescu Aruxandei D, Petrovic Stojanovska B, Penedo J, White M, Naismith J. Mechanism of DNA loading by the DNA repair helicase XPD. Nucleic Acids Res. 2016;44:2806-15 pubmed publisher
..A crystal structure of XPD from Sulfolobus acidocaldiarius that lacks helicase domain 2 has an otherwise unperturbed structure, emphasizing the stability of the interface between the Arch and 4FeS domains in XPD. ..
Sokolowski R, Graham S, White M. Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system. Nucleic Acids Res. 2014;42:6532-41 pubmed publisher
..Deep sequencing of crRNA in surveillance complexes reveals a biased distribution of spacers derived from AB and CD loci, suggesting functional coupling between Cas6 paralogues and their downstream effector complexes. ..
Peeters E, Boon M, Rollie C, Willaert R, Voet M, White M, et al
. DNA-Interacting Characteristics of the Archaeal Rudiviral Protein SIRV2_Gp1. Viruses. 2017;9: pubmed publisher
..Our findings may lead to biotechnological applications, such as the development of a toxic peptide for the containment of pathogenic bacteria, and add to our understanding of the Rudiviral infection cycle. ..