Research Topics
| Anthony D WhettonSummaryAffiliation: University of Manchester Country: UK Publications
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Publications
The time is right: proteome biology of stem cellsAnthony D Whetton
Stem Cell and Leukemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Manchester M20 9BX, UK
Cell Stem Cell 2:215-7. 2008..Here we present the Proteome Biology of Stem Cells Initiative (PBSCI) and report on its goals and future activities...
THOC5 couples M-CSF receptor signaling to transcription factor expressionLouise Carney
Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Wilmslow Road, Manchester M20 9BX, UK
Cell Signal 21:309-16. 2009..Inhibition of THOC5-induced increases in PtdInsP(3) levels abrogated the elevated levels of C/EBPbeta. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation...- Developmental fate determination and marker discovery in hematopoietic stem cell biology using proteomic fingerprintingElaine Spooncer
Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Kinnaird Road, Manchester M20 4QL, United Kingdom
Mol Cell Proteomics 7:573-81. 2008.... - Quantitative proteomics analysis demonstrates post-transcriptional regulation of embryonic stem cell differentiation to hematopoiesisAndrew J K Williamson
Stem Cell and Leukemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Kinnaird Road, Manchester M20 4QL, United Kingdom
Mol Cell Proteomics 7:459-72. 2008..ES cell development is regulated at the mRNA and protein level... - A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS)Richard D Unwin
Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medicine and Human Sciences, University of Manchester, Manchester, UK
Nat Protoc 4:870-7. 2009..With minor modifications, the MIDAS method can be applied to other protein modifications or unmodified peptides can be used as a MIDAS target... - THOC5 spliceosome protein: a target for leukaemogenic tyrosine kinases that affects inositol lipid turnoverAndrew Pierce
Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK
Br J Haematol 141:641-50. 2008..Thus, THOC5 is a novel target for modulation of signal transduction with a potential role in leukaemogenesis... - Eight-channel iTRAQ enables comparison of the activity of six leukemogenic tyrosine kinasesAndrew Pierce
Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Christie Hospital, Kinnaird House, Kinnaird Road, Manchester M204QL, United Kingdom
Mol Cell Proteomics 7:853-63. 2008.... - An assessment of peptide enrichment methods employing mTRAQ quantification approachesDavid N Potier
School of Cancer and enabling Sciences, Wolfson Molecular Imaging Centre, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK
Anal Chem 84:5604-10. 2012..We also assessed a range of methods for retaining nontryptic peptides in a plasma peptidomics workflow. From these data, we determined an optimal workflow for removing intact protein, while retaining peptides for MS-based analyses... - The use of isobaric tag peptide labeling (iTRAQ) and mass spectrometry to examine rare, primitive hematopoietic cells from patients with chronic myeloid leukemiaStephen D Griffiths
Division of Cancer Studies, Faculty of Medical and Human Sciences, Christie Hospital, University of Manchester, Wilmslow Road, Manchester M20 9BX, UK
Mol Biotechnol 36:81-9. 2007..DEAD-box protein 3, heat shock protein 105 kDa, and peroxiredoxin-3 were identified as potential protein markers for response to imatinib... - The use of proteomics for systematic analysis of normal and transformed hematopoietic stem cellsSara Cadeco
Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Manchester Academic Health Science Centre, Wolfson Molecular Imaging Centre, Manchester, UK
Curr Pharm Des 18:1730-50. 2012.... - Systematic proteome and transcriptome analysis of stem cell populationsRichard D Unwin
Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Kinnaird House, Manchester, UK
Cell Cycle 5:1587-91. 2006..The factors which must be considered when comparing two systematically acquired 'omics' datasets are reviewed and the relative merits of transcriptome and proteome approaches are discussed... - Quantitative mass spectrometry-based techniques for clinical use: biomarker identification and quantificationKathryn L Simpson
Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, United Kingdom
J Chromatogr B Analyt Technol Biomed Life Sci 877:1240-9. 2009..In this review, we discuss the MS-based methods that are available for clinical proteomic analysis and highlight the progress made and future challenges faced in this cutting edge area of research... 
Protein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cellsArkadiusz Welman
Cancer Research UK, Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom
Protein Sci 16:2711-5. 2007..Identification of potential novel phosphorylation sites will affect further functional studies of this protein, and may introduce additional complexity to PKCdelta signaling...- PEDRo: a database for storing, searching and disseminating experimental proteomics dataKevin Garwood
Department of Computer Science, University of Manchester, Oxford Road, Manchester M13 9PL, UK
BMC Genomics 5:68. 2004..However, there is, as yet, no formally accepted standard representation to support the sharing of proteomics data, and little systematic dissemination of comprehensive proteomic data sets... - The requirement for proteomics to unravel stem cell regulatory mechanismsAndrew J K Williamson
Stem Cell and Leukaemia Proteomics Laboratory, School of Cancer and enabling Sciences, Manchester Academic Health Science Centre, The University of Manchester, Christie s NHS Foundation Trust, Wolfson Molecular Imaging Centre, Withington, Manchester, UK
J Cell Physiol 226:2478-83. 2011.... - Statistical considerations of optimal study design for human plasma proteomics and biomarker discoveryCong Zhou
Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research, Manchester Academic Health Science Centre, Christie Hospital, University of Manchester, Wilmslow Road, Manchester M20 4BX, United Kingdom
J Proteome Res 11:2103-13. 2012..The power analysis provides information required prior to study initiation... - Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC-MS/MSRichard D Unwin
Stem Cell and Leukaemia Proteomics Laboratory, School of Cancer and enabling Sciences, Faculty of Medical and Human Sciences, University of Manchester, Manchester Academic Health Science Centre, Wolfson Molecular Imaging Centre, Manchester, UK
Nat Protoc 5:1574-82. 2010..This protocol discusses the rationale behind design, optimization and performance of experiments, comparing protein samples using iTRAQ chemistries combined with strong cation exchange chromatographic fractionation and MS... - Relative quantification in proteomics: new approaches for biochemistryRichard D Unwin
Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Kinnaird House, Kinnaird Road, Withington, Manchester, M20 4QL, UK
Trends Biochem Sci 31:473-84. 2006..In addition, novel flow-cytometry-based approaches are considered. These technologies can all be used to determine accurately the levels of proteins or biomarkers in a wide range of samples... - SRC-induced disassembly of adherens junctions requires localized phosphorylation and degradation of the rac activator tiam1Simon A Woodcock
Cell Signalling Group, Cancer Research UK Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
Mol Cell 33:639-53. 2009..Thus, our data implicate Tiam1 phosphorylation and consequent degradation in Src-mediated EMT and resultant cell motility and establish a paradigm for regulating local concentrations of Rho-GEFs... - How will haematologists use proteomics?Richard D Unwin
Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Kinnaird House, Kinnaird Road, Withington, Manchester, UK M20 4QL
Blood Rev 21:315-26. 2007..In this review, we discuss the technologies available for the study of the proteome that offer realistic opportunities in haematology... - Guanidination chemistry for qualitative and quantitative proteomicsStacey Warwood
Michael Barber Centre for Mass Spectrometry and Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7ND, UK
Rapid Commun Mass Spectrom 20:3245-56. 2006..In such circumstances, the additional selectivity of detection provided by MS/MS can substantiate identification and allow relative quantification of these species via product ion signal ratios... - Quantitative proteomics reveals posttranslational control as a regulatory factor in primary hematopoietic stem cellsRichard D Unwin
Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Manchester M20 4QL, UK
Blood 107:4687-94. 2006..This approach can define proteomic changes in primary samples, thereby characterizing the molecular signature of stem cells and their progeny... - Ribosome-associated nucleophosmin 1: increased expression and shuttling activity distinguishes prognostic subtypes in chronic lymphocytic leukaemiaKaren S Rees-Unwin
School of Cancer and Imaging Sciences, Faculty of Medical and Human Sciences, The University of Manchester, Manchester Academic Health Science Centre, Oxford Road, Manchester, UK
Br J Haematol 148:534-43. 2010..We propose therefore, that nucleophosmin 1, in association with ribosomal proteins, undergoes nucleo-cytoplasmic shuttling in CLL. This process is most prominent in un-mutated CLL and may signify altered protein biosynthesis... - Multiple reaction monitoring to identify sites of protein phosphorylation with high sensitivityRichard D Unwin
Faculty of Medical and Human Sciences, University of Manchester, United Kingdom
Mol Cell Proteomics 4:1134-44. 2005..The technique, which we have called multiple reaction monitoring-initiated detection and sequencing (MIDAS), is shown to be a highly sensitive approach to the determination of protein phosphorylation... - Quantitative proteomic analysis using isobaric protein tags enables rapid comparison of changes in transcript and protein levels in transformed cellsRichard D Unwin
Department of Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Withington, Manchester, M20 9BX, United Kingdom
Mol Cell Proteomics 4:924-35. 2005..Changes at the protein level in most cases reflected those seen at the transcriptome level. Nonetheless, novel differences in protein expression were found that indicate potential mechanisms for effects of this oncogene... - Global effects of BCR/ABL and TEL/PDGFRbeta expression on the proteome and phosphoproteome: identification of the Rho pathway as a target of BCR/ABLRichard D Unwin
Faculty of Medical and Human Sciences, University of Manchester
J Biol Chem 280:6316-26. 2005..Expression of a dominant-negative RhoA inhibited both DNA synthesis and transwell migration, demonstrating the significance of this pathway in BCR/ABL-mediated transformation... - Proteome biology of stem cells: a new joint HUPO and ISSCR initiativeJeroen Krijgsveld
Department of Biomolecular Mass Spectrometry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
Mol Cell Proteomics 7:204-5. 2008 - The potential for proteomic definition of stem cell populationsRichard D Unwin
Leukaemia Research Fund Proteomics Facility, Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, United Kingdom
Exp Hematol 31:1147-59. 2003..Beyond the proteome per se, advances in post-translational modification profiling mean that comparative analysis of phosphorylation patterns between stem cells and other populations can be approached... - Chronic myeloid leukaemia: an investigation into the role of Bcr-Abl-induced abnormalities in glucose transport regulationKay Barnes
School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, UK
Oncogene 24:3257-67. 2005.... - The M-CSF receptor substrate and interacting protein FMIP is governed in its subcellular localization by protein kinase C-mediated phosphorylation, and thereby potentiates M-CSF-mediated differentiationAnnalisa Mancini
Institut fur Biochemie, OE 4310, Medizinische Hochschule Hannover, Carl Neuberg Str 1, D 30623 Hannover, Germany
Oncogene 23:6581-9. 2004..We conclude M-CSF-mediated activation of PKCalpha can potentiate FMIP action to initiate survival/differentiation signaling... - Comparative proteomics of primitive hematopoietic cell populations reveals differences in expression of proteins regulating motilityCaroline A Evans
Department of Biomolecular Sciences, UMIST, Manchester, United Kingdom
Blood 103:3751-9. 2004..Adseverin was shown to be acetylated, a novel modification for this protein. Differences in major regulators of cell shape and motility between the 2 populations can explain the differential response to SDF-1... - Proteomics techniques and their application to hematologyIleana M Cristea
Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester, United Kingdom
Blood 103:3624-34. 2004..This review presents an analysis of the present and future impact of these proteomic technologies with emphasis on relative quantification for hematologic research giving an appraisal of their potential benefits... - Bcr-Abl-mediated molecular mechanism for apoptotic suppression in multipotent haemopoietic cells: a role for PKCbetaIIDia Xenaki
Biological Sciences, IENS, Lancaster University, Lancaster LA1 4YQ, UK
Cell Signal 16:145-56. 2004..However, expression of this constitutively activated PKC isoform does not suppress cytotoxic drug-induced apoptosis. Thus Bcr-Abl PTK has pleiotropic effects which can suppress cell death induced by a number of stimuli... - Changes in the proteome associated with the action of Bcr-Abl tyrosine kinase are not related to transcriptional regulationDuncan L Smith
Leukaemia Research Fund Cellular Development Unit, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester, United Kingdom
Mol Cell Proteomics 1:876-84. 2002..Leukotriene B4 levels (product of leukotriene A4 hydrolase) were, however, reduced in cells exposed long term to Bcr-Abl activity. This study demonstrates the potential of proteomic analysis to define novel effects of oncogenes... - Proteomic analyses of intermediate filaments reveals cytokeratin8 is highly acetylated--implications for colorectal epithelial homeostasisSiân H Leech
Human Nutrition Unit, Division of Clinical Sciences North, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK
Proteomics 8:279-88. 2008..Acetylation of CK8 may be associated with IFs stabilisation and thereby provide a candidate mechanism for the appropriate retention or loss of epithelial cells from the flat mucosa... - Proteomic analysis of chronic lymphocytic leukemia subtypes with mutated or unmutated Ig V(H) genesDuncan A E Cochran
Leukaemia Research Fund Proteomics Facility, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, United Kingdom
Mol Cell Proteomics 2:1331-41. 2003..The results presented show that proteomic analysis can complement other approaches in identifying proteins that may have potential value in the biological and diagnostic distinction between important clinical subtypes of CLL... - Identification of proteins from two-dimensional polyacrylamide gels using a novel acid-labile surfactantAndrew R S Ross
National Research Council of Canada, Plant Biotechnology Institute, Saskatoon SK, Canada
Proteomics 2:928-36. 2002..Automated excision and digestion procedures reduce contamination by keratin and other impurities, further enhancing MS identification of gel separated proteins...