Anthony D Whetton

Summary

Affiliation: University of Manchester
Country: UK

Publications

  1. ncbi request reprint The time is right: proteome biology of stem cells
    Anthony D Whetton
    Stem Cell and Leukemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Manchester M20 9BX, UK
    Cell Stem Cell 2:215-7. 2008
  2. doi request reprint THOC5 couples M-CSF receptor signaling to transcription factor expression
    Louise Carney
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Wilmslow Road, Manchester M20 9BX, UK
    Cell Signal 21:309-16. 2009
  3. ncbi request reprint A hierarchical statistical modeling approach to analyze proteomic isobaric tag for relative and absolute quantitation data
    Cong Zhou
    Clinical and Experimental Pharmacology Group, CRUK Manchester Institute, University of Manchester, Manchester M20 4BX, UK, Stem Cell and Leukaemia Proteomics Laboratory, Institute of Cancer Sciences, Manchester Academic Health Science Centre, Wolfson Molecular Imaging Centre, University of Manchester, Manchester M20 3LJ, UK and Centre for Biostatistics, Institute of Population Health, University of Manchester, Oxford Road, Manchester M13 9PL, UK
    Bioinformatics 30:549-58. 2014
  4. ncbi request reprint Developmental fate determination and marker discovery in hematopoietic stem cell biology using proteomic fingerprinting
    Elaine Spooncer
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Kinnaird Road, Manchester M20 4QL, United Kingdom
    Mol Cell Proteomics 7:573-81. 2008
  5. ncbi request reprint Quantitative proteomics analysis demonstrates post-transcriptional regulation of embryonic stem cell differentiation to hematopoiesis
    Andrew J K Williamson
    Stem Cell and Leukemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Kinnaird Road, Manchester M20 4QL, United Kingdom
    Mol Cell Proteomics 7:459-72. 2008
  6. ncbi request reprint Eight-channel iTRAQ enables comparison of the activity of six leukemogenic tyrosine kinases
    Andrew Pierce
    Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Christie Hospital, Kinnaird House, Kinnaird Road, Manchester M204QL, United Kingdom
    Mol Cell Proteomics 7:853-63. 2008
  7. doi request reprint THOC5 spliceosome protein: a target for leukaemogenic tyrosine kinases that affects inositol lipid turnover
    Andrew Pierce
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK
    Br J Haematol 141:641-50. 2008
  8. doi request reprint A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS)
    Richard D Unwin
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medicine and Human Sciences, University of Manchester, Manchester, UK
    Nat Protoc 4:870-7. 2009
  9. doi request reprint An assessment of peptide enrichment methods employing mTRAQ quantification approaches
    David N Potier
    School of Cancer and enabling Sciences, Wolfson Molecular Imaging Centre, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK
    Anal Chem 84:5604-10. 2012
  10. ncbi request reprint The use of isobaric tag peptide labeling (iTRAQ) and mass spectrometry to examine rare, primitive hematopoietic cells from patients with chronic myeloid leukemia
    Stephen D Griffiths
    Division of Cancer Studies, Faculty of Medical and Human Sciences, Christie Hospital, University of Manchester, Wilmslow Road, Manchester M20 9BX, UK
    Mol Biotechnol 36:81-9. 2007

Collaborators

Detail Information

Publications38

  1. ncbi request reprint The time is right: proteome biology of stem cells
    Anthony D Whetton
    Stem Cell and Leukemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Manchester M20 9BX, UK
    Cell Stem Cell 2:215-7. 2008
    ..Here we present the Proteome Biology of Stem Cells Initiative (PBSCI) and report on its goals and future activities...
  2. doi request reprint THOC5 couples M-CSF receptor signaling to transcription factor expression
    Louise Carney
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Wilmslow Road, Manchester M20 9BX, UK
    Cell Signal 21:309-16. 2009
    ..Inhibition of THOC5-induced increases in PtdInsP(3) levels abrogated the elevated levels of C/EBPbeta. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation...
  3. ncbi request reprint A hierarchical statistical modeling approach to analyze proteomic isobaric tag for relative and absolute quantitation data
    Cong Zhou
    Clinical and Experimental Pharmacology Group, CRUK Manchester Institute, University of Manchester, Manchester M20 4BX, UK, Stem Cell and Leukaemia Proteomics Laboratory, Institute of Cancer Sciences, Manchester Academic Health Science Centre, Wolfson Molecular Imaging Centre, University of Manchester, Manchester M20 3LJ, UK and Centre for Biostatistics, Institute of Population Health, University of Manchester, Oxford Road, Manchester M13 9PL, UK
    Bioinformatics 30:549-58. 2014
    ..A robust data analysis strategy is required to determine protein quantification reliability, i.e. changes due to biological regulation rather than technical variation, so that proteins that are differentially expressed can be identified...
  4. ncbi request reprint Developmental fate determination and marker discovery in hematopoietic stem cell biology using proteomic fingerprinting
    Elaine Spooncer
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Kinnaird Road, Manchester M20 4QL, United Kingdom
    Mol Cell Proteomics 7:573-81. 2008
    ....
  5. ncbi request reprint Quantitative proteomics analysis demonstrates post-transcriptional regulation of embryonic stem cell differentiation to hematopoiesis
    Andrew J K Williamson
    Stem Cell and Leukemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Kinnaird House, Kinnaird Road, Manchester M20 4QL, United Kingdom
    Mol Cell Proteomics 7:459-72. 2008
    ..ES cell development is regulated at the mRNA and protein level...
  6. ncbi request reprint Eight-channel iTRAQ enables comparison of the activity of six leukemogenic tyrosine kinases
    Andrew Pierce
    Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Christie Hospital, Kinnaird House, Kinnaird Road, Manchester M204QL, United Kingdom
    Mol Cell Proteomics 7:853-63. 2008
    ....
  7. doi request reprint THOC5 spliceosome protein: a target for leukaemogenic tyrosine kinases that affects inositol lipid turnover
    Andrew Pierce
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK
    Br J Haematol 141:641-50. 2008
    ..Thus, THOC5 is a novel target for modulation of signal transduction with a potential role in leukaemogenesis...
  8. doi request reprint A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS)
    Richard D Unwin
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medicine and Human Sciences, University of Manchester, Manchester, UK
    Nat Protoc 4:870-7. 2009
    ..With minor modifications, the MIDAS method can be applied to other protein modifications or unmodified peptides can be used as a MIDAS target...
  9. doi request reprint An assessment of peptide enrichment methods employing mTRAQ quantification approaches
    David N Potier
    School of Cancer and enabling Sciences, Wolfson Molecular Imaging Centre, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK
    Anal Chem 84:5604-10. 2012
    ..We also assessed a range of methods for retaining nontryptic peptides in a plasma peptidomics workflow. From these data, we determined an optimal workflow for removing intact protein, while retaining peptides for MS-based analyses...
  10. ncbi request reprint The use of isobaric tag peptide labeling (iTRAQ) and mass spectrometry to examine rare, primitive hematopoietic cells from patients with chronic myeloid leukemia
    Stephen D Griffiths
    Division of Cancer Studies, Faculty of Medical and Human Sciences, Christie Hospital, University of Manchester, Wilmslow Road, Manchester M20 9BX, UK
    Mol Biotechnol 36:81-9. 2007
    ..DEAD-box protein 3, heat shock protein 105 kDa, and peroxiredoxin-3 were identified as potential protein markers for response to imatinib...
  11. ncbi request reprint The use of proteomics for systematic analysis of normal and transformed hematopoietic stem cells
    Sara Cadeco
    Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Manchester Academic Health Science Centre, Wolfson Molecular Imaging Centre, Manchester, UK
    Curr Pharm Des 18:1730-50. 2012
    ....
  12. ncbi request reprint Systematic proteome and transcriptome analysis of stem cell populations
    Richard D Unwin
    Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Kinnaird House, Manchester, UK
    Cell Cycle 5:1587-91. 2006
    ..The factors which must be considered when comparing two systematically acquired 'omics' datasets are reviewed and the relative merits of transcriptome and proteome approaches are discussed...
  13. doi request reprint Quantitative mass spectrometry-based techniques for clinical use: biomarker identification and quantification
    Kathryn L Simpson
    Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, United Kingdom
    J Chromatogr B Analyt Technol Biomed Life Sci 877:1240-9. 2009
    ..In this review, we discuss the MS-based methods that are available for clinical proteomic analysis and highlight the progress made and future challenges faced in this cutting edge area of research...
  14. pmc PEDRo: a database for storing, searching and disseminating experimental proteomics data
    Kevin Garwood
    Department of Computer Science, University of Manchester, Oxford Road, Manchester M13 9PL, UK
    BMC Genomics 5:68. 2004
    ..However, there is, as yet, no formally accepted standard representation to support the sharing of proteomics data, and little systematic dissemination of comprehensive proteomic data sets...
  15. doi request reprint The requirement for proteomics to unravel stem cell regulatory mechanisms
    Andrew J K Williamson
    Stem Cell and Leukaemia Proteomics Laboratory, School of Cancer and enabling Sciences, Manchester Academic Health Science Centre, The University of Manchester, Christie s NHS Foundation Trust, Wolfson Molecular Imaging Centre, Withington, Manchester, UK
    J Cell Physiol 226:2478-83. 2011
    ....
  16. pmc Protein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cells
    Arkadiusz Welman
    Cancer Research UK, Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom
    Protein Sci 16:2711-5. 2007
    ..Identification of potential novel phosphorylation sites will affect further functional studies of this protein, and may introduce additional complexity to PKCdelta signaling...
  17. pmc Statistical considerations of optimal study design for human plasma proteomics and biomarker discovery
    Cong Zhou
    Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research, Manchester Academic Health Science Centre, Christie Hospital, University of Manchester, Wilmslow Road, Manchester M20 4BX, United Kingdom
    J Proteome Res 11:2103-13. 2012
    ..The power analysis provides information required prior to study initiation...
  18. doi request reprint Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC-MS/MS
    Richard D Unwin
    Stem Cell and Leukaemia Proteomics Laboratory, School of Cancer and enabling Sciences, Faculty of Medical and Human Sciences, University of Manchester, Manchester Academic Health Science Centre, Wolfson Molecular Imaging Centre, Manchester, UK
    Nat Protoc 5:1574-82. 2010
    ..This protocol discusses the rationale behind design, optimization and performance of experiments, comparing protein samples using iTRAQ chemistries combined with strong cation exchange chromatographic fractionation and MS...
  19. ncbi request reprint Relative quantification in proteomics: new approaches for biochemistry
    Richard D Unwin
    Stem Cell and Leukaemia Proteomics Laboratory, University of Manchester, Kinnaird House, Kinnaird Road, Withington, Manchester, M20 4QL, UK
    Trends Biochem Sci 31:473-84. 2006
    ..In addition, novel flow-cytometry-based approaches are considered. These technologies can all be used to determine accurately the levels of proteins or biomarkers in a wide range of samples...
  20. doi request reprint SRC-induced disassembly of adherens junctions requires localized phosphorylation and degradation of the rac activator tiam1
    Simon A Woodcock
    Cell Signalling Group, Cancer Research UK Paterson Institute for Cancer Research, University of Manchester, Manchester, UK
    Mol Cell 33:639-53. 2009
    ..Thus, our data implicate Tiam1 phosphorylation and consequent degradation in Src-mediated EMT and resultant cell motility and establish a paradigm for regulating local concentrations of Rho-GEFs...
  21. ncbi request reprint How will haematologists use proteomics?
    Richard D Unwin
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Kinnaird House, Kinnaird Road, Withington, Manchester, UK M20 4QL
    Blood Rev 21:315-26. 2007
    ..In this review, we discuss the technologies available for the study of the proteome that offer realistic opportunities in haematology...
  22. ncbi request reprint Guanidination chemistry for qualitative and quantitative proteomics
    Stacey Warwood
    Michael Barber Centre for Mass Spectrometry and Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7ND, UK
    Rapid Commun Mass Spectrom 20:3245-56. 2006
    ..In such circumstances, the additional selectivity of detection provided by MS/MS can substantiate identification and allow relative quantification of these species via product ion signal ratios...
  23. doi request reprint Ribosome-associated nucleophosmin 1: increased expression and shuttling activity distinguishes prognostic subtypes in chronic lymphocytic leukaemia
    Karen S Rees-Unwin
    School of Cancer and Imaging Sciences, Faculty of Medical and Human Sciences, The University of Manchester, Manchester Academic Health Science Centre, Oxford Road, Manchester, UK
    Br J Haematol 148:534-43. 2010
    ..We propose therefore, that nucleophosmin 1, in association with ribosomal proteins, undergoes nucleo-cytoplasmic shuttling in CLL. This process is most prominent in un-mutated CLL and may signify altered protein biosynthesis...
  24. ncbi request reprint Quantitative proteomics reveals posttranslational control as a regulatory factor in primary hematopoietic stem cells
    Richard D Unwin
    Stem Cell and Leukaemia Proteomics Laboratory, Faculty of Medical and Human Sciences, University of Manchester, Manchester M20 4QL, UK
    Blood 107:4687-94. 2006
    ..This approach can define proteomic changes in primary samples, thereby characterizing the molecular signature of stem cells and their progeny...
  25. ncbi request reprint Multiple reaction monitoring to identify sites of protein phosphorylation with high sensitivity
    Richard D Unwin
    Faculty of Medical and Human Sciences, University of Manchester, United Kingdom
    Mol Cell Proteomics 4:1134-44. 2005
    ..The technique, which we have called multiple reaction monitoring-initiated detection and sequencing (MIDAS), is shown to be a highly sensitive approach to the determination of protein phosphorylation...
  26. ncbi request reprint Quantitative proteomic analysis using isobaric protein tags enables rapid comparison of changes in transcript and protein levels in transformed cells
    Richard D Unwin
    Department of Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Withington, Manchester, M20 9BX, United Kingdom
    Mol Cell Proteomics 4:924-35. 2005
    ..Changes at the protein level in most cases reflected those seen at the transcriptome level. Nonetheless, novel differences in protein expression were found that indicate potential mechanisms for effects of this oncogene...
  27. ncbi request reprint Global effects of BCR/ABL and TEL/PDGFRbeta expression on the proteome and phosphoproteome: identification of the Rho pathway as a target of BCR/ABL
    Richard D Unwin
    Faculty of Medical and Human Sciences, University of Manchester
    J Biol Chem 280:6316-26. 2005
    ..Expression of a dominant-negative RhoA inhibited both DNA synthesis and transwell migration, demonstrating the significance of this pathway in BCR/ABL-mediated transformation...
  28. doi request reprint Proteome biology of stem cells: a new joint HUPO and ISSCR initiative
    Jeroen Krijgsveld
    Department of Biomolecular Mass Spectrometry, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Mol Cell Proteomics 7:204-5. 2008
  29. ncbi request reprint The potential for proteomic definition of stem cell populations
    Richard D Unwin
    Leukaemia Research Fund Proteomics Facility, Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, United Kingdom
    Exp Hematol 31:1147-59. 2003
    ..Beyond the proteome per se, advances in post-translational modification profiling mean that comparative analysis of phosphorylation patterns between stem cells and other populations can be approached...
  30. ncbi request reprint Comparative proteomics of primitive hematopoietic cell populations reveals differences in expression of proteins regulating motility
    Caroline A Evans
    Department of Biomolecular Sciences, UMIST, Manchester, United Kingdom
    Blood 103:3751-9. 2004
    ..Adseverin was shown to be acetylated, a novel modification for this protein. Differences in major regulators of cell shape and motility between the 2 populations can explain the differential response to SDF-1...
  31. ncbi request reprint Changes in the proteome associated with the action of Bcr-Abl tyrosine kinase are not related to transcriptional regulation
    Duncan L Smith
    Leukaemia Research Fund Cellular Development Unit, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester, United Kingdom
    Mol Cell Proteomics 1:876-84. 2002
    ..Leukotriene B4 levels (product of leukotriene A4 hydrolase) were, however, reduced in cells exposed long term to Bcr-Abl activity. This study demonstrates the potential of proteomic analysis to define novel effects of oncogenes...
  32. ncbi request reprint Proteomic analysis of chronic lymphocytic leukemia subtypes with mutated or unmutated Ig V(H) genes
    Duncan A E Cochran
    Leukaemia Research Fund Proteomics Facility, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, United Kingdom
    Mol Cell Proteomics 2:1331-41. 2003
    ..The results presented show that proteomic analysis can complement other approaches in identifying proteins that may have potential value in the biological and diagnostic distinction between important clinical subtypes of CLL...
  33. doi request reprint Proteomic analyses of intermediate filaments reveals cytokeratin8 is highly acetylated--implications for colorectal epithelial homeostasis
    Siân H Leech
    Human Nutrition Unit, Division of Clinical Sciences North, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK
    Proteomics 8:279-88. 2008
    ..Acetylation of CK8 may be associated with IFs stabilisation and thereby provide a candidate mechanism for the appropriate retention or loss of epithelial cells from the flat mucosa...
  34. ncbi request reprint Bcr-Abl-mediated molecular mechanism for apoptotic suppression in multipotent haemopoietic cells: a role for PKCbetaII
    Dia Xenaki
    Biological Sciences, IENS, Lancaster University, Lancaster LA1 4YQ, UK
    Cell Signal 16:145-56. 2004
    ..However, expression of this constitutively activated PKC isoform does not suppress cytotoxic drug-induced apoptosis. Thus Bcr-Abl PTK has pleiotropic effects which can suppress cell death induced by a number of stimuli...
  35. ncbi request reprint Proteomics techniques and their application to hematology
    Ileana M Cristea
    Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, Manchester, United Kingdom
    Blood 103:3624-34. 2004
    ..This review presents an analysis of the present and future impact of these proteomic technologies with emphasis on relative quantification for hematologic research giving an appraisal of their potential benefits...
  36. ncbi request reprint Chronic myeloid leukaemia: an investigation into the role of Bcr-Abl-induced abnormalities in glucose transport regulation
    Kay Barnes
    School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, UK
    Oncogene 24:3257-67. 2005
    ....
  37. ncbi request reprint The M-CSF receptor substrate and interacting protein FMIP is governed in its subcellular localization by protein kinase C-mediated phosphorylation, and thereby potentiates M-CSF-mediated differentiation
    Annalisa Mancini
    Institut fur Biochemie, OE 4310, Medizinische Hochschule Hannover, Carl Neuberg Str 1, D 30623 Hannover, Germany
    Oncogene 23:6581-9. 2004
    ..We conclude M-CSF-mediated activation of PKCalpha can potentiate FMIP action to initiate survival/differentiation signaling...
  38. ncbi request reprint Identification of proteins from two-dimensional polyacrylamide gels using a novel acid-labile surfactant
    Andrew R S Ross
    National Research Council of Canada, Plant Biotechnology Institute, Saskatoon SK, Canada
    Proteomics 2:928-36. 2002
    ..Automated excision and digestion procedures reduce contamination by keratin and other impurities, further enhancing MS identification of gel separated proteins...