Finn Werner

Summary

Affiliation: University College London
Country: UK

Publications

  1. ncbi request reprint Structure and function of archaeal RNA polymerases
    Finn Werner
    University College London, Department of Biochemistry and Molecular Biology, Darwin Building, Gower Street, London WC1E 6BT, UK
    Mol Microbiol 65:1395-404. 2007
  2. pmc Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif
    Angela Hirtreiter
    Division of Biosciences, University College London, Institute for Structural and Molecular Biology, Darwin Building, Gower Street, London WC1E 6BT, UK
    Nucleic Acids Res 38:4040-51. 2010
  3. pmc The initiation factor TFE and the elongation factor Spt4/5 compete for the RNAP clamp during transcription initiation and elongation
    Dina Grohmann
    University College London, Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK
    Mol Cell 43:263-74. 2011
  4. ncbi request reprint Structural evolution of multisubunit RNA polymerases
    Finn Werner
    Research Department of Structural and Molecular Biology, University College London, Gower Street, London, UK
    Trends Microbiol 16:247-50. 2008
  5. ncbi request reprint Evolution of multisubunit RNA polymerases in the three domains of life
    Finn Werner
    RNA Polymerase Laboratory, Institute for Structural and Molecular Biology, Division of Biosciences, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    Nat Rev Microbiol 9:85-98. 2011
  6. doi request reprint RNA-binding to archaeal RNA polymerase subunits F/E: a DEER and FRET study
    Dina Grohmann
    Institute of Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom
    J Am Chem Soc 132:5954-5. 2010
  7. doi request reprint Molecular mechanisms of archaeal RNA polymerase
    Dina Grohmann
    UCL Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK
    Biochem Soc Trans 37:12-7. 2009
  8. pmc Molecular mechanisms of RNA polymerase--the F/E (RPB4/7) complex is required for high processivity in vitro
    Angela Hirtreiter
    Division of Biosciences, Institute for Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    Nucleic Acids Res 38:585-96. 2010
  9. doi request reprint FRET (fluorescence resonance energy transfer) sheds light on transcription
    Dina Grohmann
    RNAP Laboratory, UCL Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK
    Biochem Soc Trans 39:122-7. 2011
  10. doi request reprint RNAP subunits F/E (RPB4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro
    Dina Grohmann
    Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK
    Biochem J 421:339-43. 2009

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Structure and function of archaeal RNA polymerases
    Finn Werner
    University College London, Department of Biochemistry and Molecular Biology, Darwin Building, Gower Street, London WC1E 6BT, UK
    Mol Microbiol 65:1395-404. 2007
    ....
  2. pmc Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif
    Angela Hirtreiter
    Division of Biosciences, University College London, Institute for Structural and Molecular Biology, Darwin Building, Gower Street, London WC1E 6BT, UK
    Nucleic Acids Res 38:4040-51. 2010
    ..Using a mutagenesis approach, we have identified a hydrophobic pocket on the Spt5 NGN domain as binding site for RNAP, and reciprocally the RNAP clamp coiled-coil motif as binding site for Spt4/5...
  3. pmc The initiation factor TFE and the elongation factor Spt4/5 compete for the RNAP clamp during transcription initiation and elongation
    Dina Grohmann
    University College London, Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK
    Mol Cell 43:263-74. 2011
    ..During elongation, Spt4/5 can displace TFE from the RNAP elongation complex and stimulate processivity. Our results identify the RNAP "clamp" region as a regulatory hot spot for both transcription initiation and transcription elongation...
  4. ncbi request reprint Structural evolution of multisubunit RNA polymerases
    Finn Werner
    Research Department of Structural and Molecular Biology, University College London, Gower Street, London, UK
    Trends Microbiol 16:247-50. 2008
    ..Two reports from the Murakami and Cramer laboratories have now filled this gap in our knowledge and enable us to hypothesize about the evolution of the structure and function of RNAPs...
  5. ncbi request reprint Evolution of multisubunit RNA polymerases in the three domains of life
    Finn Werner
    RNA Polymerase Laboratory, Institute for Structural and Molecular Biology, Division of Biosciences, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    Nat Rev Microbiol 9:85-98. 2011
    ....
  6. doi request reprint RNA-binding to archaeal RNA polymerase subunits F/E: a DEER and FRET study
    Dina Grohmann
    Institute of Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom
    J Am Chem Soc 132:5954-5. 2010
    ..Our results demonstrate that, upon binding of RNA, F/E remains in a stable conformation, which suggests that it serves as a structurally rigid guiding rail for the growing RNA chain during transcription...
  7. doi request reprint Molecular mechanisms of archaeal RNA polymerase
    Dina Grohmann
    UCL Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK
    Biochem Soc Trans 37:12-7. 2009
    ....
  8. pmc Molecular mechanisms of RNA polymerase--the F/E (RPB4/7) complex is required for high processivity in vitro
    Angela Hirtreiter
    Division of Biosciences, Institute for Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK
    Nucleic Acids Res 38:585-96. 2010
    ..In summary, our results suggest that interactions between RNAP subunits F/E and the RNA transcript are pivotal to the molecular mechanisms of RNAP during transcription elongation and termination...
  9. doi request reprint FRET (fluorescence resonance energy transfer) sheds light on transcription
    Dina Grohmann
    RNAP Laboratory, UCL Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, Gower Street, London WC1E 6BT, UK
    Biochem Soc Trans 39:122-7. 2011
    ....
  10. doi request reprint RNAP subunits F/E (RPB4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro
    Dina Grohmann
    Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, University College London, Gower Street, London WC1E 6BT, UK
    Biochem J 421:339-43. 2009
    ..We have characterized the molecular interactions between the F/E complex and the RNAP core. F/E binds to RNAP with submicromolar affinity and is not in a dynamic exchange with unbound F/E...
  11. doi request reprint Cycling through transcription with the RNA polymerase F/E (RPB4/7) complex: structure, function and evolution of archaeal RNA polymerase
    Dina Grohmann
    RNAP Laboratory, Institute for Structural and Molecular Biology, University College London, Division of Biosciences, Darwin Building, Gower Street, London WC1E6BT, UK
    Res Microbiol 162:10-8. 2011
    ..F/E is highly versatile, it facilitates DNA strand-separation during transcription initiation, increases processivity during the elongation phase of transcription and ensures efficient transcription termination...
  12. pmc Hold on!: RNA polymerase interactions with the nascent RNA modulate transcription elongation and termination
    Dina Grohmann
    UCL Institute for Structural and Molecular Biology, Division of Biosciences, London, UK
    RNA Biol 7:310-5. 2010
    ..Interestingly, bacteriophage-encoded antiterminator proteins could, in theory, fulfil an analogous function in the bacterial RNAP...
  13. doi request reprint Archaeology of RNA polymerase: factor swapping during the transcription cycle
    Fabian Blombach
    RNAP Laboratory, University College London, Institute of Structural and Molecular Biology, Division of Biosciences, Gower Street, London WC1E 6BT, UK
    Biochem Soc Trans 41:362-7. 2013
    ..Therefore we propose that this factor-swapping mechanism, operating in all three domains of life, is the outcome of convergent evolution...
  14. ncbi request reprint Promoter independent abortive transcription assays unravel functional interactions between TFIIB and RNA polymerase
    Simone C Wiesler
    Department of Life Sciences, Imperial College London, London, UK
    Methods Mol Biol 977:217-27. 2013
    ..These assays can be used to measure the effect of transcription factors such as TFIIB and RNAP mutations on abortive transcription...
  15. pmc A nexus for gene expression-molecular mechanisms of Spt5 and NusG in the three domains of life
    Finn Werner
    RNAP Laboratory, Institute of Structural and Molecular Biology, Division of Biosciences, University College London, Gower Street, London WC1E 6BT, UK
    J Mol Biol 417:13-27. 2012
    ..This review discusses recent discoveries that identify Spt5-like factors as evolutionary conserved nexus for the regulation and coordination of the machineries responsible for information processing in the cell...
  16. doi request reprint Recent advances in the understanding of archaeal transcription
    Dina Grohmann
    RNAP Laboratory, Institute for Structural and Molecular Biology, Division of Biosciences, University College London, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom
    Curr Opin Microbiol 14:328-34. 2011
    ..This review covers recent discoveries about the mechanisms of archaeal transcription obtained by a combination of in vivo and in vitro approaches, from the molecular to the global level...
  17. pmc Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis
    Chris P Ryan
    Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK
    Chem Commun (Camb) 47:5452-4. 2011
    ..The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described...
  18. pmc Direct modulation of RNA polymerase core functions by basal transcription factors
    Finn Werner
    Department of Biological Sciences, Division of Cell and Molecular Biology, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom
    Mol Cell Biol 25:8344-55. 2005
    ..iv) An additional function of TFE is to dynamically alter the nucleic acid-binding properties of RNAP by stabilizing the initiation complex and destabilizing elongation complexes...
  19. ncbi request reprint A fully recombinant system for activator-dependent archaeal transcription
    Mohamed Ouhammouch
    Division of Biological Sciences and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093 0634, USA
    J Biol Chem 279:51719-21. 2004
    ....
  20. ncbi request reprint A recombinant RNA polymerase II-like enzyme capable of promoter-specific transcription
    Finn Werner
    Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London, United Kingdom
    Mol Cell 10:635-46. 2002
    ..The assembly of mutant enzymes yielded several unexpected insights into the structural and functional contributions of various subunits toward overall RNAP activity...
  21. pmc Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II
    Hedije Meka
    Department of Biological Sciences, Imperial College London, SW7 2BW, UK
    Nucleic Acids Res 33:6435-44. 2005
    ..The area spans the nucleic acid binding face of the OB fold, including the B4-B5 loop, but also extends towards the N-terminal domain...
  22. pmc Structural and functional homology between the RNAP(I) subunits A14/A43 and the archaeal RNAP subunits E/F
    Hedije Meka
    Department of Biological Sciences, Imperial College, Exhibition Road, London SW7 2AZ, UK
    Nucleic Acids Res 31:4391-400. 2003
    ..To shed light on the function of the heterodimer, we performed gel mobility shift assays and showed that the A14/A43 heterodimer binds single-stranded RNA in a similar way to the archaeal E/F complex...