Kira J Weissman

Summary

Affiliation: University of Cambridge
Country: UK

Publications

  1. ncbi The structure of docking domains in modular polyketide synthases
    R William Broadhurst
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA, Cambridge, United Kingdom
    Chem Biol 10:723-31. 2003
  2. ncbi Single amino acid substitutions alter the efficiency of docking in modular polyketide biosynthesis
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 7:1334-42. 2006
  3. doi Biochemistry. Anatomy of a fungal polyketide synthase
    Kira J Weissman
    Department of Pharmaceutical Biotechnology, Saarland University, 60041 Saarbrücken, Germany
    Science 320:186-7. 2008
  4. ncbi Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 5:116-25. 2004
  5. ncbi Polyketide biosynthesis: understanding and exploiting modularity
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Philos Trans A Math Phys Eng Sci 362:2671-90. 2004
  6. ncbi Combinatorial biosynthesis of reduced polyketides
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK
    Nat Rev Microbiol 3:925-36. 2005
  7. ncbi Evidence for a protein-protein interaction motif on an acyl carrier protein domain from a modular polyketide synthase
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Chem Biol 13:625-36. 2006
  8. ncbi The structural basis for docking in modular polyketide biosynthesis
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 7:485-94. 2006
  9. doi Insights into protein-protein and enzyme-substrate interactions in modular polyketide synthases
    Lucky Tran
    Department of Biochemistry, 80 Tennis Court Road, University of Cambridge, Cambridge CB21GA, UK
    Chem Biol 17:705-16. 2010
  10. ncbi Multienzyme docking in hybrid megasynthetases
    Carsten D Richter
    Department of Biochemistry, 80 Tennis Court Road, University of Cambridge, Cambridge CB2 1GA, UK
    Nat Chem Biol 4:75-81. 2008

Collaborators

Detail Information

Publications22

  1. ncbi The structure of docking domains in modular polyketide synthases
    R William Broadhurst
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA, Cambridge, United Kingdom
    Chem Biol 10:723-31. 2003
    ....
  2. ncbi Single amino acid substitutions alter the efficiency of docking in modular polyketide biosynthesis
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 7:1334-42. 2006
  3. doi Biochemistry. Anatomy of a fungal polyketide synthase
    Kira J Weissman
    Department of Pharmaceutical Biotechnology, Saarland University, 60041 Saarbrücken, Germany
    Science 320:186-7. 2008
  4. ncbi Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 5:116-25. 2004
    ..coli. The efficiency of the SePptII in phosphopantetheinyl transfer in E. coli makes it an attractive alternative to other Sfp-type PPTases for co-expression experiments with PKS proteins...
  5. ncbi Polyketide biosynthesis: understanding and exploiting modularity
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Philos Trans A Math Phys Eng Sci 362:2671-90. 2004
    ....
  6. ncbi Combinatorial biosynthesis of reduced polyketides
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK
    Nat Rev Microbiol 3:925-36. 2005
    ..So far, directed engineering of modular PKSs has resulted in the production of more than 200 new polyketides, but key challenges remain before the potential of combinatorial biosynthesis can be fully realized...
  7. ncbi Evidence for a protein-protein interaction motif on an acyl carrier protein domain from a modular polyketide synthase
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Chem Biol 13:625-36. 2006
    ..Our results accord with previous studies of discrete ACP proteins from fatty acid and aromatic polyketide biosynthesis, suggesting that helix II may also serve as a universal interaction motif in modular PKSs...
  8. ncbi The structural basis for docking in modular polyketide biosynthesis
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 7:485-94. 2006
    ..The results of these helix swaps are fully consistent with the model and highlight residues in the docking domains that may be targeted to alter the efficiency or specificity of subunit-subunit docking in hybrid PKSs...
  9. doi Insights into protein-protein and enzyme-substrate interactions in modular polyketide synthases
    Lucky Tran
    Department of Biochemistry, 80 Tennis Court Road, University of Cambridge, Cambridge CB21GA, UK
    Chem Biol 17:705-16. 2010
    ....
  10. ncbi Multienzyme docking in hybrid megasynthetases
    Carsten D Richter
    Department of Biochemistry, 80 Tennis Court Road, University of Cambridge, Cambridge CB2 1GA, UK
    Nat Chem Biol 4:75-81. 2008
    ..The pattern of charged residues on the contact surface of the beta-hairpin is a key determinant of the interaction and seems to constitute a 'docking code' that can be used to alter binding affinity...
  11. ncbi Ketoreduction in mycolactone biosynthesis: insight into substrate specificity and stereocontrol from studies of discrete ketoreductase domains in vitro
    Shilpa Bali
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
    Chembiochem 7:1935-42. 2006
    ..In addition, our findings reinforce the role of substrate tethering for achieving stereochemical control in modular PKSs by affecting the delicate energetics of ketoreduction...
  12. doi Covalent linkage mediates communication between ACP and TE domains in modular polyketide synthases
    Lucky Tran
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 9:905-15. 2008
    ....
  13. ncbi Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137
    Barbara M Harvey
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Chem Biol 14:703-14. 2007
    ..Consistent with this, deletion of either monACPX or monKSX from the monensin gene cluster effectively abolished monensin A biosynthesis...
  14. ncbi Autonomous folding of interdomain regions of a modular polyketide synthase
    Carsten D Richter
    Department of Biochemistry, University of Cambridge, UK
    FEBS J 274:2196-209. 2007
    ....
  15. ncbi Broad substrate specificity of ketoreductases derived from modular polyketide synthases
    Shilpa Bali
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Chembiochem 7:478-84. 2006
    ..These results suggest that PKS KRs could potentially be used as biotransformation catalysts for the production of chiral alcohols...
  16. ncbi Molecular basis of Celmer's rules: stereochemistry of catalysis by isolated ketoreductase domains from modular polyketide synthases
    Alexandros P Siskos
    Department of Biochemistry, University of Cambridge, UK
    Chem Biol 12:1145-53. 2005
    ..These data, together with modeling of diketide binding to KR(1) and KR(2), demonstrate the fine energetic balance between alternative modes of presentation of ketoacylthioester substrates to KR active sites...
  17. ncbi Mutasynthesis - uniting chemistry and genetics for drug discovery
    Kira J Weissman
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
    Trends Biotechnol 25:139-42. 2007
    ..Recently, this technique has been exploited by Cambridge-based biotech company Biotica Technology Ltd, and their collaborators, to generate promising new variants of the polyketide anti-cancer compounds rapamycin and borrelidin...
  18. doi Protein-protein interactions in multienzyme megasynthetases
    Kira J Weissman
    Pharmaceutical Biotechnology, Saarland University, PO Box 151150, 66041 Saarbrucken, Germany
    Chembiochem 9:826-48. 2008
    ....
  19. ncbi Evidence for the mode of action of the highly cytotoxic Streptomyces polyketide kendomycin
    Yasser A Elnakady
    Department of Pharmaceutical Biotechnology, Saarland University, P O Box 151150, 66041 Saarbrucken, Germany
    Chembiochem 8:1261-72. 2007
    ..This study therefore provides evidence that kendomycin mediates its cytotoxic effects, at least in part, through proteasome inhibition...
  20. doi Myxochelin biosynthesis: direct evidence for two- and four-electron reduction of a carrier protein-bound thioester
    Yanyan Li
    Pharmaceutical Biotechnology, Saarland University, P O Box 151150, 66041 Saarbrucken, Germany
    J Am Chem Soc 130:7554-5. 2008
    ..Finally, we show that the relative levels of myxochelin A and B are likely to be controlled by the direct competition of MxcL and the MxcG Red domain for a free aldehyde intermediate...
  21. doi DKxanthene biosynthesis--understanding the basis for diversity-oriented synthesis in myxobacterial secondary metabolism
    Peter Meiser
    Institut für Pharmazeutische Biotechnologie, Saarland University, Saarbrucken, Germany
    Chem Biol 15:771-81. 2008
    ....
  22. ncbi Biosynthesis of (R)-beta-tyrosine and its incorporation into the highly cytotoxic chondramides produced by Chondromyces crocatus
    Shwan Rachid
    Institute for Pharmaceutical Biotechnology, Saarland University, Saarbrucken, Germany
    J Biol Chem 282:21810-7. 2007
    ..Comparison to the (S)-beta-tyrosine specific A domain SgcC1 should enhance our understanding of the structural and stereochemical determinants guiding amino acid selection by non-ribosomal peptide synthetase multienzymes...