Research Topics
Genomes and Genes | Kira J WeissmanSummaryAffiliation: University of Cambridge Country: UK Publications
| Collaborators
|
Detail Information
Publications
The structure of docking domains in modular polyketide synthasesR William Broadhurst
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA, Cambridge, United Kingdom
Chem Biol 10:723-31. 2003....- Single amino acid substitutions alter the efficiency of docking in modular polyketide biosynthesisKira J Weissman
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Chembiochem 7:1334-42. 2006 - Biochemistry. Anatomy of a fungal polyketide synthaseKira J Weissman
Department of Pharmaceutical Biotechnology, Saarland University, , Germany
Science 320:186-7. 2008 - Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraeaKira J Weissman
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Chembiochem 5:116-25. 2004..coli. The efficiency of the SePptII in phosphopantetheinyl transfer in E. coli makes it an attractive alternative to other Sfp-type PPTases for co-expression experiments with PKS proteins... - Polyketide biosynthesis: understanding and exploiting modularityKira J Weissman
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Philos Transact A Math Phys Eng Sci 362:2671-90. 2004.... - Combinatorial biosynthesis of reduced polyketidesKira J Weissman
Department of Biochemistry, University of Cambridge, Cambridge, CB2 1GA, UK
Nat Rev Microbiol 3:925-36. 2005..So far, directed engineering of modular PKSs has resulted in the production of more than 200 new polyketides, but key challenges remain before the potential of combinatorial biosynthesis can be fully realized... - Evidence for a protein-protein interaction motif on an acyl carrier protein domain from a modular polyketide synthaseKira J Weissman
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
Chem Biol 13:625-36. 2006..Our results accord with previous studies of discrete ACP proteins from fatty acid and aromatic polyketide biosynthesis, suggesting that helix II may also serve as a universal interaction motif in modular PKSs... - The structural basis for docking in modular polyketide biosynthesisKira J Weissman
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Chembiochem 7:485-94. 2006..The results of these helix swaps are fully consistent with the model and highlight residues in the docking domains that may be targeted to alter the efficiency or specificity of subunit-subunit docking in hybrid PKSs... 
Insights into protein-protein and enzyme-substrate interactions in modular polyketide synthasesLucky Tran
Department of Biochemistry, 80 Tennis Court Road, University of Cambridge, Cambridge CB21GA, UK
Chem Biol 17:705-16. 2010....- Multienzyme docking in hybrid megasynthetasesCarsten D Richter
Department of Biochemistry, 80 Tennis Court Road, University of Cambridge, Cambridge CB2 1GA, UK
Nat Chem Biol 4:75-81. 2008..The pattern of charged residues on the contact surface of the beta-hairpin is a key determinant of the interaction and seems to constitute a 'docking code' that can be used to alter binding affinity... - Covalent linkage mediates communication between ACP and TE domains in modular polyketide synthasesLucky Tran
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Chembiochem 9:905-15. 2008.... - Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137Barbara M Harvey
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
Chem Biol 14:703-14. 2007..Consistent with this, deletion of either monACPX or monKSX from the monensin gene cluster effectively abolished monensin A biosynthesis... - Autonomous folding of interdomain regions of a modular polyketide synthaseCarsten D Richter
Department of Biochemistry, University of Cambridge, UK
FEBS J 274:2196-209. 2007.... - Ketoreduction in mycolactone biosynthesis: insight into substrate specificity and stereocontrol from studies of discrete ketoreductase domains in vitroShilpa Bali
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
Chembiochem 7:1935-42. 2006..In addition, our findings reinforce the role of substrate tethering for achieving stereochemical control in modular PKSs by affecting the delicate energetics of ketoreduction... - Broad substrate specificity of ketoreductases derived from modular polyketide synthasesShilpa Bali
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Chembiochem 7:478-84. 2006..These results suggest that PKS KRs could potentially be used as biotransformation catalysts for the production of chiral alcohols... - Molecular basis of Celmer's rules: stereochemistry of catalysis by isolated ketoreductase domains from modular polyketide synthasesAlexandros P Siskos
Department of Biochemistry, University of Cambridge, UK
Chem Biol 12:1145-53. 2005..These data, together with modeling of diketide binding to KR(1) and KR(2), demonstrate the fine energetic balance between alternative modes of presentation of ketoacylthioester substrates to KR active sites... - Mutasynthesis - uniting chemistry and genetics for drug discoveryKira J Weissman
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK
Trends Biotechnol 25:139-42. 2007..Recently, this technique has been exploited by Cambridge-based biotech company Biotica Technology Ltd, and their collaborators, to generate promising new variants of the polyketide anti-cancer compounds rapamycin and borrelidin... - Protein-protein interactions in multienzyme megasynthetasesKira J Weissman
Pharmaceutical Biotechnology, Saarland University, PO Box 151150, 66041 Saarbrucken, Germany
Chembiochem 9:826-48. 2008.... - Evidence for the mode of action of the highly cytotoxic Streptomyces polyketide kendomycinYasser A Elnakady
Department of Pharmaceutical Biotechnology, Saarland University, P O Box 151150, 66041 Saarbrucken, Germany
Chembiochem 8:1261-72. 2007..This study therefore provides evidence that kendomycin mediates its cytotoxic effects, at least in part, through proteasome inhibition... - Biosynthesis of (R)-beta-tyrosine and its incorporation into the highly cytotoxic chondramides produced by Chondromyces crocatusShwan Rachid
Institute for Pharmaceutical Biotechnology, Saarland University, Saarbrucken, Germany
J Biol Chem 282:21810-7. 2007..Comparison to the (S)-beta-tyrosine specific A domain SgcC1 should enhance our understanding of the structural and stereochemical determinants guiding amino acid selection by non-ribosomal peptide synthetase multienzymes... - Myxochelin biosynthesis: direct evidence for two- and four-electron reduction of a carrier protein-bound thioesterYanyan Li
Pharmaceutical Biotechnology, Saarland University, P O Box 151150, 66041 Saarbrucken, Germany
J Am Chem Soc 130:7554-5. 2008..Finally, we show that the relative levels of myxochelin A and B are likely to be controlled by the direct competition of MxcL and the MxcG Red domain for a free aldehyde intermediate... - DKxanthene biosynthesis--understanding the basis for diversity-oriented synthesis in myxobacterial secondary metabolismPeter Meiser
Institut für Pharmazeutische Biotechnologie, Saarland University, Saarbrucken, Germany
Chem Biol 15:771-81. 2008....